scholarly journals The Antibacterial Effect of Ethanol Extract of Polish Propolis on Mutans Streptococci and Lactobacilli Isolated from Saliva

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Arkadiusz Dziedzic ◽  
Robert Kubina ◽  
Robert D. Wojtyczka ◽  
Agata Kabała-Dzik ◽  
Marta Tanasiewicz ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Mutans Streptococci ◽  
Diffusion Method ◽  
Extracellular Polysaccharides ◽  
Cariogenic Bacteria ◽  
Slide Test ◽  
The Mean ◽  
Alamarblue Assay

Dental caries occurrence is caused by the colonization of oral microorganisms and accumulation of extracellular polysaccharides synthesized byStreptococcus mutanswith the synergistic influence ofLactobacillusspp. bacteria. The aim of this study was to determineex vivothe antibacterial properties of ethanol extract of propolis (EEP), collected in Poland, against the main cariogenic bacteria: salivary mutans streptococci and lactobacilli. The isolation of mutans streptococci group bacteria (MS) andLactobacillusspp. (LB) from stimulated saliva was performed by in-office CRT bacteria dip slide test. The broth diffusion method and AlamarBlue assay were used to evaluate the antimicrobial activity of EEP, with the estimation of its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The biochemical composition of propolis components was assessed. The mean MIC and MBC values of EEP, in concentrations ranging from 25 mg/mL to 0.025 mg/mL, for the MS and LB were found to be 1.10 mg/mLversus0.7 mg/mL and 9.01 mg/mLversus5.91 mg/mL, respectively. The exposure to an extract of Polish propolis affected mutans streptococci andLactobacillusspp. viability, exhibiting an antibacterial efficacy on mutans streptococci group bacteria and lactobacilli saliva residents, while lactobacilli were more susceptible to EEP. Antibacterial measures containing propolis could be the local agents acting against cariogenic bacteria.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN FRAKSI KULIT BATANG BELIMBING WULUH (Averrhoa bilimbi Linn.) TERHADAP BAKTERI Klebsiella pneumoniae DAN Staphylococcus epidermidis BESERTA BIOAUTOGRAFINYA

Biomedika ◽  
2012 ◽  
Vol 4 (2) ◽  
Author(s):  
Dr. Muhtadi , MSi. ◽  
Ria Ambarwati ◽  
Ratna Yuliani
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Water Fraction

Belimbing wuluh (Averrhoa bilimbi Linn.) is a tropical plant that has antibacterial properties. The purpose of this study was to test the antibacterial activity of bark Belimbing wuluh against Klebsiella pneumoniae and Staphylococcus epidermidis and their bioautography. Extraction methods used to research is method maceration with a solvent ethanol 96 %. Fractinations done by method partition liquid-liquid with a separating funnel. Test performed in this research covering identi� cation bacteria, the sensitivity bacteria, antibacterial activity, thin layer chromatography, bioautography. The result of antibacterial activity ethanol extract of disk diffusion method with concentrations 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8±0,5; 10,34±0,58; 12,17±0,76 on Klebsiella pneumoniae, 10,17±0,29; 11±0; 11.5±0 on Staphylococcus epidermidis, n-hexane fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8,34±0,29; 9,34±0,29; 10,84±0,76 on Klebsialla pneumoniae, 8,5±0,5; 9,34±0,29; 10,67±0,29 on Staphylococcus epidermidis, ethyl acetate fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 9,17±0,29; 10,34±0,29; 11,17±0,29 on Klebsiella pneumoniae and 9,5±0,5; 10,67±0,29; 12,67±1,26 on Staphylococcus epidermidis, ethanol-water fractions with concentration 400 μg/disk, 800 μg/ disk, 1600 μg/disk is 8,17±0,29; 9,17±0,29; 10±0 on Klebsiella pneumoniae, 9±0; 9,67±0,29; 10,34±0,29 on Staphylococcus epidermidis. The TLC show chemical compounds contained in the ethanol extract, n-heksan fraction, ethyl acetate fraction, and ethanol-water fraction is a compound of the saponins, alkaloids, � avonoids and phenolic. Bioautography showed that ethanol extracts, n-heksan faction, ethyl acetate fraction, and etanol-airfaction Belimbing wuluh (Averrhoa bilimbi Linn.) bark have not antibacterial activity because there is no clear area around on plate TLC.Keywords: Belimbing wuluh (Averrhoa bilimbi Linn.), ethanol extract, fractination, antibacterial, bioautogra� .

Effect of Synthesized Ag Nanoparticles by Using the Different Amounts of Polyvinylpyrrolidone for Ag-Natural Rubber Hybrid Sheets and their Antibacterial Properties

2017 ◽  
Vol 751 ◽  
pp. 270-276 ◽  
Author(s):  
Warot Prasanseang ◽  
Chaval Sriwong ◽  
Kittisak Choojun
Keyword(s):  
Natural Rubber ◽  
Low Cost ◽  
Antibacterial Properties ◽  
Rubber Matrix ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Zones Of Inhibition ◽  
Ft Ir ◽  
The Mean

Ag-natural rubber (Ag-NR) hybrid sheets were successfully prepared with a very simple and low cost method. In this method, silver nanoparticles (AgNPs) were firstly synthesized by a rapid and green microwave-assisted using polyvinylpyrroridone (PVP) media. The effect of PVP weight ratios towards the size of AgNPs was also investigated. Then, Ag-NR hybrid sheet samples were prepared by latex mixing-casting method using concentrated natural rubber (NR) latex with the synthesized AgNPs colloids. The characteristic absorption, particles sizes and shapes of the obtained AgNPs were examined through UV-vis, TEM and SAED. Also, the prepared Ag-NR sheet samples were characterized using XRD, FT-IR, SEM and EDS techniques. It was found that the particles sizes of all the synthesized AgNPs had spherical-like shape, and the mean sizes were increased from 29.7 to 90.4 nm upon increasing PVP contents. EDS results showed the AgNPs were well-dispersed and impregnated into the rubber matrix. Moreover, the antibacterial properties of the prepared Ag-NR sheets were tested by agar disk-diffusion method with Gram-positive and Gram-negative bacteria as Staphylococcus aureus(S. aureus) and Escherichia coli(E. coli), respectively. The results showed that the hybrid sheets exhibited excellent antibacterial properties against these bacteria, in which the zones of inhibition were also dependent on the synthesized AgNPs by utilizing the different amounts of PVP.

scholarly journals Gut Extracts of Rhynchophorus ferrugineus Larvae Olivier Affecting Bacterial Dental Caries

2021 ◽  
Author(s):  
G.H. Sewify ◽  
H.M. Hamada ◽  
Z.A. Mustafa ◽  
Hani A. Alhadrami
Keyword(s):  
Dental Caries ◽  
Morphological Changes ◽  
Bacterial Species ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Rhynchophorus Ferrugineus ◽  
Electron Microscopic ◽  
Inhibition Zones ◽  
The Mean ◽  
Klebsiella Spp

In vitro study was conducted to explore antibacterial properties of the larval gut extracts of Rhynchophorus ferrugineus (Red Palm Weevil) Oliver. Larval gut extracts were tested against salivary bacteria causing dental carries using the agar well diffusion method. The gut extracts significantly affected the growth of both Klebsiella spp. and Streptococcus viridans. The two bacterial species revealed significant differences in their sensitivity to the extract. The extract efficacy depended upon the concentration and time of exposure. When using 100%concentration of the extract, the mean of inhibition zones for S. viridans and Klebsiella spp. at 24 h after treatment were 1.61 mm and 2.50 mm, respectively. At 48 h post-treatment, the mean of inhibition zones for S. viridans and Klebsiella spp. were 1.96 mm and 2.66 mm. After 72 hours, the means zones were 2.28 mm and 2.91 mm, respectively. Electron microscopic examinations showed morphological changes of the outer membrane of bacteria with a noticeable damage as a result of exposure to the gut extract. The results suggest potential use of these extracts against dental caries bacteria.

Antibacterial Effect of New Bioceramic Pulp Capping Material on the Main Cariogenic Bacteria

2016 ◽  
Vol 17 (5) ◽  
pp. 349-353 ◽  
Author(s):  
Fahiem MM Elshamy ◽  
Husham Elraih ◽  
Iti Gupta ◽  
Faisal AI Idris
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Effect ◽  
Antibacterial Properties ◽  
Mineral Trioxide Aggregate ◽  
Mutans Streptococci ◽  
Repair Material ◽  
Cariogenic Bacteria ◽  
Pulp Capping ◽  
Root Repair ◽  
Significant Difference

ABSTRACT Background The purpose of this research was to assess the antibacterial activity of a new bioceramic pulp capping material (endosequence root repair material [ERRM]) against the main cariogenic bacteria: Salivary mutans streptococci (MS) and lactobacilli and compare the results with mineral trioxide aggregate (MTA) and calcium hydroxide (Dycal). Materials and methods The isolation of MS group bacteria and Lactobacillus (LB) spp. from stimulated saliva was performed with in-office caries risk test bacteria dip slide test. Endosequence root repair material, MTA (ProRoot MTA), and Dycal were used as pulp capping materials. Mutans Streptococci and LB were scattered on the agar dishes with a swab. The pulp capping materials under study were placed in the wells and prepared in the agar, immediately after mixing. The dishes were incubated for 24 hours at 37°C. The growth inhibition zones were recorded and compared for every material and bacterial strain. One-way analysis of variance test was done to compare the development of growth inhibition of selected bacteria against testing materials. Post hoc Tukey honest significant difference was conducted to compare each material group. Results All the three selected pulp capping materials were found to inhibit the bacteria LB and MS. The antibacterial activity of ERRM and ProRoot MTA was significantly better than the Dycal. Against MS, ERRM and MTA showed no statistically significant difference. Mineral trioxide aggregate showed significantly better inhibitory activity against LB. Conclusion Endosequence root repair material and MTA had superior antibacterial properties against the main cariogenic bacteria: MS and LB compared with Dycal. Clinical significance A pulp-capping agent having good antibacterial properties can have better success rate in maintaining the vitality of the tooth while treating deep carious lesions in patients. How to cite this article Elshamy FMM, Singh G, Elraih H, Gupta I, AI Idris F. Antibacterial Effect of New Bioceramic Pulp Capping Material on the Main Cariogenic Bacteria. J Contemp Dent Pract 2016;17(5):349-353.

scholarly journals Impact of decalcification on antibacterial properties of eggshell against selected poultry pathogens

2021 ◽  
Vol 22 (2) ◽  
pp. 204-210
Author(s):  
T.V. Balogu ◽  
B.C. Chukwueze ◽  
T.P. Okonkwo
Keyword(s):  
Antibacterial Effect ◽  
Antibacterial Properties ◽  
Fine Powder ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Mean Values ◽  
Significance Level ◽  
E Coli ◽  
Zone Diameter ◽  
The Mean

Background: Eggshell which is primarily composed of more than 98% calcium carbonate crystal, serves as the physical protective and active barrier structure of egg content. Recently, antimicrobial properties of eggshell are fast becoming center of interest among stakeholders of poultry industry. However, few studies have focused on the rigidity factor of calcium components of eggshell as antimicrobial agent. Thus, this study was designed to determine the effect of decalcification on the ability of eggshell to inhibit common poultry and egg bacterial pathogens.Methods: Raw eggshell denoted as calcified eggshell (CES) and decalcified eggshell (DES) were extracted and made into fine powder. Standard protocol was used for preparations of CES and DES at concentrations of 10, 5, 2.5 and 1.25 mg/ml, and their antibacterial assays on selected bacterial pathogens (Bacillus subtilis, Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli and Salmonella Typhi) were performed by agar diffusion method. Gentamicin 80mg solution (CC1) and distilled water (CC2) served as controls. Data were analysed with SPSS version 20.0 and presented as mean±SD for descriptive statistics. Friedman's two-way test ANOVA was used to compare the differences in mean values between CES, DES, CC1 and CC2 at significance level of p<0.05.Results: The mean zone diameter of inhibition produced by DES (range 13–28mm) for the isolates was significantly higher (p<0.05) than that produced by CES (range 10-21mm). However, the mean zone diameter of inhibition produced by CC1 (gentamicin) (range 16-40mm) was higher than that produced by DES or CES (p<0.05). The concentrations of DES and CES have no significant antibacterial effect on B. subtilis and K. pneumoniae (p>0.05), but had inverse effect on P. aeruginosa. Overall, DES had a better inhibitory effect than CES against B. subtilis, K. pneumoniae and P. aeruginosa, but notably, neither DES nor CES had inhibitory effect on E. coli and S. Typhi.Conclusion: Poor antibacterial effect of CES may be attributed to the calcium-protein interactions within bacterial cell membrane, which hinders absorption or mobility mechanism of the antibacterial factor of the eggshell, but decalcification had significant impact on the antibacterial profile of the eggshell for some bacterial isolates. However, S. Typhi and E. coli were totally resistant to both DES and CES. Breed of eggs with minimal calcified eggshell to withstand transportation fragility, may enhance antibacterial index and shelf-life of table eggs. Keywords: Decalcification; Antibacterial; Eggshell; Poultry; Pathogens

scholarly journals Comparison of Antimicrobial Activity of Fluoride Varnishes Against Streptococcusmutans and Lactobacillus acidophilus: An In Vitro Study

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Banafsheh Soleimani ◽  
Hamidreza Goli ◽  
Mahsa Naranjian ◽  
Seyed Jaber Mousavi ◽  
Azam Nahvi
Keyword(s):  
Lactobacillus Acidophilus ◽  
Antibacterial Properties ◽  
In Vitro Study ◽  
Inhibition Zone ◽  
Vitro Study ◽  
Diffusion Method ◽  
P Value ◽  
Antibacterial Effects ◽  
The Mean

Background: Topical fluoride products such as varnishes prevent dental caries by limiting demineralization and antibacterial properties. The structural and metabolic characteristics of cariogenic microorganisms are different from each other. Also, the formulation properties, concentration, and release behavior of fluoride vary in different varnishes. Objectives: In this study, we investigated the antibacterial effects of three types of common fluoride varnishes against two cariogenic bacteria of Streptococcus mutans (S.M.) and Lactobacillus acidophilus (L.A.). Methods: In this in vitro study, antibacterial effects of MI varnish [containing calcium phosphopeptide-amorphous calcium phosphate (CPP-ACP)], Polimo (containing xylitol), and FluoroDose varnishes were evaluated using disc diffusion method via measuring the diameter of the inhibition zone. The antibiotics of erythromycin and ampicillin were used as the positive control of the test. Analysis of variance (ANOVA) and post hoc tests were applied to compare the mean of non-growth zone diameter in the studied groups. Data were analyzed by SPSS 16. The statistical significance level was determined as P-value < 0.05. Results: The antibacterial effect of each varnish was optimal against both bacteria. In comparison, the mean diameter of the inhibition zone in MI varnish was significantly (P-value=0.019) higher than Polimo and FluoroDose brands on S.M.; however, this difference was not statistically significant for L.A. Furthermore, Polimo and FluoroDose varnishes showed similar antimicrobial effects against these bacteria. Conclusions: The use of these varnishes seems to be suitable for preventing tooth decay. MI varnish is preferable because of its higher antibacterial properties.

scholarly journals In Vitro Antibacterial Activity, Preliminary Phytochemical Screening Profile, and In Vivo Toxicity of Seven Traditional Medicinal Plants in Ethiopia

2022 ◽  
Author(s):  
Eyerus Mekuriaw ◽  
Enat Mengistu ◽  
Ayana Erdedo ◽  
Hassen Mamo
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Ethyl Acetate ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Phytochemical Content

The threat of antibiotic-resistance calls for novel antibacterial agents. This study was aimed at screening medicinal plants for their antibacterial properties, phytochemical content and safety. Leaves of Allophylus abyssinicus (Hochst.) Radlk., Dicliptera laxata C.B.Clarke, Ligustrum vulgare L., Solanecio gigas (Vatke) c. Jeffrey and Gymnanthemum myrianthum (Hook.f.) H.Rob.; leaf and stem-bark of Olinia rochetiana A. Juss. and the seed of Cucurbita pepo L. were used. Chloroform and ethanol were used to extract G. myrianthum, D. laxata and O. rochetiana; ethyl acetate and methanol for the rest, and water for all. The extracts were tested against clinical/standard strains of Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi and Staphylococcus aureus by the agar-diffusion method. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined. Acute toxicity to mice was checked and preliminary phytochemical screening was done. Thirteen extracts, out of 24, were active (inhibition zone >7 mm) at differing levels (9.67±0.33-25.66±0.57 mm) against at least one bacterial strain. The MICs and MBCs were 1.95-15.6 mg/mL and 7.8-125 mg/mL respectively. The aqueous extract of S. gigas, methanol extracts of L. vulgare and A. abyssinicus, and ethanol extract of O. rochetiana leaf were the most active (MIC 1.95mg/ml) against S. aureus. Ethyl acetate extracts of A. abyssinicus, L. vulgare and S. gigas; aqueous of C. pepo, O. rochetiana and G. myrianthum; and all D. laxata had no antibacterial activity. P. aeruginosa was the least susceptible to any extract, although the methanol and aqueous extracts of S. gigas performed better against it. Preliminary phytochemical screening of selected extracts for phenols, flavonoids, tannins, steroids, terpenoids, steroidal glycosides, alkaloids, saponins, resins and glycosides showed positivity at least for four of these phytochemicals with glycoside and terpenoids in nearly all extracts and resin in none. The plants were not toxic to mice at 2000 mg/kg. Further consideration of S. gigas, L. vulgare, A. abyssinicus and O. rochetiana is recommended in light of their promising potential and safety.

scholarly journals Isolation of Alkaloid Compounds from Ethanol Extract of Rimpang Galang Merah (Alpinia purpurata (Vielli) K. Schum) and nanoparticle production from its Alkaloid Extract. Comparative Study of Antibacterial Properties on Staphylococcus aureus and Eschericia

2018 ◽  
Vol 21 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Vatara Artanta Silalahi ◽  
Enny Fachriyah ◽  
Pratama Jujur Wibawa
Keyword(s):  
Ethyl Acetate ◽  
Average Particle Size ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Ammonium Hydroxide ◽  
Diffusion Method ◽  
Average Particle ◽  
Scattering Method ◽  
Visible Absorption ◽  
Dynamic Light Scattering Method

Isolation of alkaloid compounds from ethanol extract of red galangal rhizomes to compare their antibacterial properties with extracts formed into nanoparticles has been performed. The isolation of the alkaloid compounds was performed successively by salting using (i) hydrochloric acid (HCl) to pH 3 (when in ethanol) and using (ii) ammonium hydroxide (NH4OH) to pH 10 (when in chloroform). The alkaloid concentrated extract was obtained after chloroform was evaporated using a rotary evaporator. This alkaloid extract was then carried out by TLC silica gel GF254 using chloroform eluent: ethyl acetate (9: 2) to obtain a specific alkaloid type as a single/pure compound. Test of alkaloid purity successfully isolated by one-dimensional or two-dimensional TLC using various types of eluents with varying degrees of polarity, ie acetone, chloroform, ethyl acetate, ethanol and chloroform: ethyl acetate mixture (9: 2). Isolates of pure alkaloids were analyzed using UV-Vis spectrophotometers), FTIR and (LC-MS). The obtained alkaloid isolates were isokuinolin group with UV-visible absorption characteristics at maximum wavelength (λmaks) of 212 nm, 227 nm and 261 nm. The alkaloid isolate also showed the presence of functional groups -OH, -C-H, C = O, C = C, C = N, C-N, and C-O with molecular weight of 262.90 g/mol. The production of ethanol extract nanoparticles containing alkaloid-containing red galangal (EEAlkNPs) was performed using a top-down approach using ultrason (40 kHz, 2x50 watt) for 10 minutes. The EEAlkNPs size was determined by the dynamic light scattering method (DLS) using the Particles size analyzer (PSA) tool and obtained an average particle size of 220.2 nm. The antibacterial properties of EEAlkNPs were tested by disc diffusion method for the culture of S.aureus and E.coli bacteria. It is known that the antibacterial properties of EEAlkNPs are relatively larger than the original ethanol extract at the same concentration, ie, 2000 parts per million (bpj) or g / L.

scholarly journals Comparison extraction methods for evaluating antioxidant and antibacterial properties of Vernonia amygdalina leaves extract

2020 ◽  
Vol 10 (8) ◽  
pp. 775
Author(s):  
Muharni Muharni ◽  
Elfita Elfita ◽  
Heni Yohandini ◽  
Chika Valenta
Keyword(s):  
Antibacterial Activity ◽  
Antioxidant Properties ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Extraction Methods ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Flavonoid Content

In this study, we were interested in comparing the influence of different extraction methods on antioxidant and antibacterial activities of <em>V.amygdalina</em> leaves extracts. The extracts were also analyzed for their total phenolic and flavonoid content.  The extraction methods used maceration, soxhlet, and fractionation, the evaluation of antioxidant activity using DPPH (1,1-diphenyl-2-picryl-hydrazyl) method and antibacterial activity by agar diffusion method. The total phenolic and flavonoids are determined by the spectrophotometric method.  Ethanol extract by fractionating on this method showed the highest antioxidant properties compared to other extracts with IC<sub>50 </sub>170 μg/mL in category potent antioxidant with total phenolic content 28.83 ± 2.62 mg GAE/g, and the total flavonoid content 18.78 ± 0.15 mg QE/g. Evaluation of antibacterial properties the extracts of <em>V. amyigdalina</em> showed moderate antibacterial activity against all bacteria tests with inhibition zone  6.1 ± 0.1 to  9.4 ± 0.9 mm at variation concentration 62.5 – 1000 μg/mL. The fractionation method using ethanol solvent is the best for the extraction of antioxidant compounds from <em>V. amygdalina</em>.

scholarly journals UJI AKTIVITAS ANTIBAKTERI SEDIAAN SABUN CAIR EKSTRAK ETANOL DAUN CENGKEH (Syzygium aromaticum) TERHADAP BAKTERI Staphylococcus aureus

PHARMACON ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 767
Author(s):  
Evander Roliand Lomboan ◽  
Paulina V.Y. Yamlean ◽  
Elly J. Suoth
Keyword(s):  
Staphylococcus Aureus ◽  
Specific Gravity ◽  
Moisture Content ◽  
Foam Height ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Syzygium Aromaticum ◽  
Liquid Soap

ABSTRACT Clove leaf (Syzygium aromaticum) is a plant that contains eugenol, saponins, flavonoids and tannins which have antibacterial properties. This study aims to formulate the ethanol extract liquid soap of clove leaves and test the effectiveness of the preparation against Staphylococcus aureus bacteria. This research was conducted in a laboratory experiment to make liquid soap with extract concentrations of 4%, 7%, 9%, 12% and 15%, then the soap was tested for spesifications including organoleptic testing, pH, foam height, moisture content, free alkaline content, and specific gravity. Antibacterial effectiveness testing against the growth of Staphylococcus aureus was carried out using the diffusion method. Clove leaf ethanol extract (Syzygium aromaticum) can be formulated into liquid soap. The results of testing the quality of liquid soap preparations meet the requirements in accordance with SNI. The results obtained from the antibacterial effectiveness test of liquid soap ethanol extract of Clove leaves can inhibit Staphylococcus aureus bacteria with all concentrations in the strong category, with a concentration of 15% having the largest diameter of 17,33 mm. Keywords : Antibacterial, Liquid Soap, Syzygium aromaticum, Staphylococcus aureus ABSTRAK Daun Cengkeh (Syzygium aromaticum) merupakan tanaman yang memiliki kandungan eugenol, saponin, flavonoid dan tannin yang bersifat sebagai antibakteri. Penelitian ini bertujuan untuk memformulasi sabun cair ekstrak etanol daun Cengkeh dan pengujian efektivitas sediaan terhadap bakteri Stahpylococcus aureus. Penelitian ini dilakukan secara eksperimental laboratorium untuk membuat sabun cair dengan konsentrasi ekstrak yaitu 4%, 7%, 9%, 12%, dan 15%, selanjutnya sabun diuji spesifikasinya meliputi pengujian organoleptik, pH, tinggi busa, kadar air, kadar alkali bebas, dan bobot jenis. Pengujian efektivitas antibakteri terhadap pertumbuhan Staphylococcus aureus dilakukan dengan menggunakan metode difusi. Ekstrak etanol daun Cengkeh (Syzygium aromaticum) dapat diformulasikan menjadi sabun cair. Hasil pengujian mutu sediaan sabun cair memenuhi persyaratan sesuai dengan SNI. Hasil uji efektivitas antibakteri sabun cair ekstrak etanol daun Cengkeh yang diperoleh dapat menghambat bakteri Staphylococcus aureus dengan semua konsentrasinya termasuk dalam kategori kuat, dengan konsentrasi 15% yang memiliki diameter paling besar yaitu 17,33 mm. Kata Kunci: Antibakteri, Sabun Cair, Syzygium aromaticum , Staphylococcus aureus

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