scholarly journals Fungal and Bacterial Communities in the Rhizosphere ofPinus tabulaeformisRelated to the Restoration of Plantations and Natural Secondary Forests in the Loess Plateau, Northwest China

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Hong-Xia Yu ◽  
Chun-Yan Wang ◽  
Ming Tang
Keyword(s):  
Loess Plateau ◽  
Bacterial Communities ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Bacterial Species ◽  
Soil Microbial Communities ◽  
Principal Component ◽  
Terrestrial Ecosystems ◽  
Pinus Tabulaeformis ◽  
Secondary Forests ◽  
The Loess Plateau

Chinese pine (Pinus tabulaeformisCarr.) is widely planted for restoration in destroyed ecosystems of the Loess Plateau in China. Although soil microbial communities are important subsurface components of the terrestrial ecosystems, little is known about fungal and bacterial communities in the rhizosphere of planted and naturalP. tabulaeformisforests in the region. In this study, fungal and bacterial communities in the rhizosphere ofP. tabulaeformiswere analyzed by nested PCR-DGGE (denaturing gradient gel electrophoresis). Diversity analysis revealed that the values of the Shannon-Wiener index (H) and the Simpson index (D) of fungal communities were higher in natural secondary forests than in plantations except for the 3-year-old site. Moreover, the values of species richness,H, andDof the bacterial communities were also higher in the former. Totally, 18 fungal and 19 bacterial DGGE band types were successfully retrieved and sequenced. The dominant fungi in the rhizosphere ofP. tabulaeformisbelonged to the phylum of Basidiomycota, while the dominant bacteria belonged to the phylum of Proteobacteria. Principal component analysis indicated that fungal and bacterial species were more unitary in plantations than in natural secondary forests, and the majority of them were more likely to appear in the latter. Correlation analysis showed no significant correlation between the fungal and bacterial community diversities.

Assembly mechanisms of soil bacterial communities in subalpine coniferous forests on the Loess Plateau, China

2019 ◽  
Vol 57 (6) ◽  
pp. 461-469
Author(s):  
Pengyu Zhao ◽  
Jinxian Liu ◽  
Tong Jia ◽  
Zhengming Luo ◽  
Cui Li ◽  
...  
Keyword(s):  
Loess Plateau ◽  
Bacterial Communities ◽  
Coniferous Forests ◽  
The Loess Plateau ◽  
Soil Bacterial Communities ◽  
Soil Bacterial

scholarly journals Screening of bacterial populations in crop rotations with different proportion of cereals

2014 ◽  
Vol 60 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Katarína Ondreičková ◽  
Mária Babulicová ◽  
Daniel Mihálik ◽  
Marcela Gubišová ◽  
Jozef Gubiš
Keyword(s):  
Bacterial Communities ◽  
Fragment Length Polymorphism ◽  
Bacterial Species ◽  
Principal Component ◽  
Crop Rotations ◽  
Community Diversity ◽  
Bacterial Populations ◽  
Organic Fertiliser ◽  
Two Samples ◽  
Significant Difference

Abstract Terminal restriction fragment length polymorphism was used to determine the bacterial community diversity in crop rotations with different proportion of cereals (40%, 60% and 80%) and various fertilisation treatments (F1 - mineral fertiliser amendment + the application of organic fertiliser Veget® and F2 - mineral fertilisation) sampled on two dates (July and October 2012). No statistically significant differences in the number of terminal restriction fragments were detected by Fisher´s least significant difference between two types of fertilisation or among samples with different proportion of cereals. In contrast, statistically significant differences were detected between samples collected in July and October, when in October there was a 43.5% reduction in the number of bacterial species in comparison with July. Principal component analysis as well as cluster analysis showed that a higher similarity in composition of bacterial communities was present among all soil samples collected in July and two samples collected in October. Other samples collected in October were separated from each other. The results of this study demonstrated that bacteria abundance and community composition were not affected by the proportion of cereals and fertiliser used.

scholarly journals Phyllosphere and carposphere bacterial communities in olive plants subjected to different cultural practices

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Silvia Pascazio ◽  
Carmine Crecchio ◽  
Patrizia Ricciuti ◽  
Assunta Maria Palese ◽  
Cristos Xiloyannis ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Bacterial Communities ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Cultural Practices ◽  
Plant Protection ◽  
Bacterial Species ◽  
Agricultural Practices ◽  
Fruit Pulp ◽  
Gradient Gel Electrophoresis ◽  
Olive Trees

The aim of this study was to characterize phyllosphere and carposphere bacterial communities of olive trees subjected for 13 years to two different soil management systems (sustainable and conventional) in a mature olive grove located in Southern Italy. Amplified DNA fragments of the 16S ribosomal RNA eubacterial gene (16S <em>rRNA</em>) of bacteria living on leaf and fruit surface, and in fruit pulp were analyzed by denaturing gradient gel electrophoresis (DGGE). A clone library of 16S <em>rRNA</em> amplicons extracted from the bacteria living in pulp homogenates and a phylogenetic analysis were performed. Generally, the DGGE patterns of the bacteria from both the treatments clustered separately. The medium-term sustainable orchard management resulted in a higher number of bacterial species from olive fruit pulp. Phyllosphere and carposphere communities evaluated by DGGE were affected by the type of the agricultural practices adopted. A better understanding of phyllosphere and carposphere microbiota of cultivated olive plants could be useful for the promotion of plant growth, a better plant protection and a higher crop quality.

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