scholarly journals Aqueous Extract ofSolanum nigrumLeaves Induces Autophagy and Enhances Cytotoxicity of Cisplatin, Doxorubicin, Docetaxel, and 5-Fluorouracil in Human Colorectal Carcinoma Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Chen-Jei Tai ◽  
Chien-Kai Wang ◽  
Cheng-Jeng Tai ◽  
Yi-Feng Lin ◽  
Chi-Shian Lin ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Colorectal Carcinoma ◽  
Aqueous Extract ◽  
Tumor Suppression ◽  
Drug Effect ◽  
Carcinoma Cells ◽  
Common Cancer ◽  
Human Colorectal Carcinoma ◽  
And Control ◽  
Ht 29

Colorectal cancer is a common cancer worldwide, and chemotherapy is a mainstream approach for advanced and recurrent cases. Development of effective complementary drugs could help improve tumor suppression efficiency and control adverse effects from chemotherapy. The aqueous extract ofSolanum nigrumleaves (AE-SN) is an essential component in many traditional Chinese medicine formulas for treating cancer, but there is a lack of evidence verifying its tumor suppression efficacy in colorectal cancer. The purpose of this study is to evaluate the tumor suppression efficacy of AE-SN using DLD-1 and HT-29 human colorectal carcinoma cells and examine the combined drug effect when combined with the chemotherapeutic drugs cisplatin, doxorubicin, docetaxel, and 5-fluorouracil. The results indicated that AE-SN induced autophagy via microtubule-associated protein 1 light chain 3 A/B II accumulation but not caspase-3-dependent apoptosis in both cell lines. The IC50s after 48 hours of treatment were 0.541 and 0.948 mg/ml AE-SN in DLD-1 and HT-29, respectively. AE-SN also demonstrated a combined drug effect with all tested drugs by enhancing cytotoxicity in tumor cells. Our results suggest that AE-SN has potential in the development of complementary chemotherapy for colorectal cancer.

scholarly journals High Concentrations of Aspartame Induce Pro-Angiogenic Effects in Ovo and Cytotoxic Effects in HT-29 Human Colorectal Carcinoma Cells

Nutrients ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3600 ◽  
Author(s):  
Anca Laura Maghiari ◽  
Dorina Coricovac ◽  
Iulia Andreea Pinzaru ◽  
Ioana Gabriela Macașoi ◽  
Iasmina Marcovici ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Colorectal Carcinoma ◽  
In Vitro Cytotoxicity ◽  
Scientific Data ◽  
Carcinoma Cells ◽  
Artificial Sweetener ◽  
In Ovo ◽  
Human Colorectal Carcinoma ◽  
Ht 29

Aspartame (ASP), an artificial sweetener abundantly consumed in recent years in an array of dietary products, has raised some concerns in terms of toxicity, and it was even suggested a link with the risk of carcinogenesis (colorectal cancer), though the present scientific data are rather inconclusive. This study aims at investigating the potential role of aspartame in colorectal cancer by suggesting two experimental approaches: (i) an in vitro cytotoxicity screening in HT-29 human colorectal carcinoma cells based on cell viability (Alamar blue assay), cell morphology and cell migration (scratch assay) assessment and (ii) an in ovo evaluation in terms of angiogenic and irritant potential by means of the chorioallantoic membrane method (CAM). The in vitro results showed a dose-dependent cytotoxic effect, with a significant decrease of viable cells at the highest concentrations tested (15, 30 and 50 mM) and morphological cellular changes. In ovo, aspartame (15 and 30 mM) proved to have a pro-angiogenic effect and a weak irritant potential at the vascular level. These data suggest new directions of research regarding aspartame’s role in colorectal cancer.

scholarly journals Biosynthesis of Fe nanoparticles using Alhagi sparsifolia extract for the treatment of colorectal carcinoma in the in vitro condition: A pre-clinical trial study

2021 ◽  
Author(s):  
Yadong Zhou ◽  
Fafu Dou ◽  
Tahani Awad Alahmadi ◽  
Sulaiman Ali Alharbi ◽  
Milton Wainwright
Keyword(s):  
Colorectal Carcinoma ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Mtt Assay ◽  
Iron Nanoparticles ◽  
Alhagi Sparsifolia ◽  
Leaf Aqueous Extract ◽  
Hct 116 ◽  
Human Colorectal Carcinoma ◽  
Ht 29

IntroductionThe preparation and formulation of new chemotherapeutic supplements and drugs with remarkable effects for the treatment of cancer are in the priority of both developing and developed countries. Recently, iron nanoparticles have been used as modern chemotherapeutic drugs for the treatment of several cancers such as leukemia, lung cancer, breast cancer, prostate cancer, etc. In the present study, iron nanoparticles were green-synthesized using the aqueous extract of Alhagi sparsifolia leaf aqueous extract.Material and methodsThe synthesized FeNPs were characterized by analytical techniques including SEM, TEM, UV-Vis., and FT-IR. The anti-human colorectal carcinoma activity of FeNPs was evaluated using MTT assay. In the cellular and molecular part of the recent study, the treated cells with FeNPs were assessed by MTT assay for 48h about the cytotoxicity and anti-human colorectal carcinoma properties on normal (HUVEC) and colorectal carcinoma cell lines i.e. HT-29, HCT 116, HCT-8, and Ramos.2G6.4C10.ResultsThe nanoparticles were formed in a spherical shape in the average size of 47.24 nm. In the antioxidant test, the IC50 of FeNPs and BHT against DPPH free radicals were 161 and 134 µg/mL, respectively. The viability of malignant colorectal cell line reduced dose-dependently in the presence of FeNPs. The IC50 of FeNPs were 250, 293, 276, and 344 µg/mL against HT-29, HCT 116, HCT-8, and Ramos.2G6.4C10 cell lines, respectively.ConclusionsAfter clinical study, iron nanoparticles containing Alhagi sparsifolia leaf aqueous extract may be used to formulate a new chemotherapeutic drug or supplement to treat the several types of human colorectal carcinoma.

scholarly journals Calendula officinalis green-mediated silver nanoparticles: Formulation, characterization and assessment of colorectal cancer activities green-mediated silver nanoparticles: Formulation, characterization and assessment of colorectal cancer activities

2021 ◽  
Author(s):  
Yongchao Xu ◽  
Behnam Mahdavi ◽  
Mohammad Zangeneh ◽  
Akram Zangeneh ◽  
Maryam Qorbani ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Silver Nanoparticles ◽  
Colorectal Carcinoma ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Mtt Assay ◽  
Analytical Techniques ◽  
Spherical Shape ◽  
Calendula Officinalis ◽  
Human Colorectal Carcinoma

IntroductionThe biosynthesis of metal nanoparticles using medicinal plants is not only economical but also environmentally friendly as well as having miscellaneous biomedical applications. In the present study, silver nanoparticles were green-synthesized using the aqueous extract of Calendula officinalis.Material and methodsThe synthesized AgNPs@Calendula officinalis were characterized by analytical techniques including EDX, FE-SEM, XRD, UV-Vis., and FT-IR. The anti-human colorectal cancer activities of AgNPs@Calendula officinalis were evaluated using MTT assay.ResultsThe nanoparticles were formed in a spherical shape in the range of 38.05 to 75.41 nm for the particle size. On the other hand, the MTT assay was run to evaluate anti colorectal cancer activity of AgNPs@Calendula officinalis. In the cellular and molecular part of the recent study, the treated cells with AgNPs@Calendula officinalis were assessed by MTT assay for 48 h about the cytotoxicity and anti-human colorectal carcinoma properties on normal (HUVEC) and colorectal carcinoma cell lines i.e. WiDr, SW1417 [SW-1417], and DLD-1. In the antioxidant test, the IC50 of AgNPs@Calendula officinalis and BHT against DPPH free radicals were 222 and 124 µg/mL, respectively. The viability of malignant colorectal cell line reduced dose-dependently in the presence of AgNPs@Calendula officinalis. The IC50 of AgNPs@Calendula officinalis were 430, 326, and 392 µg/mL against WiDr, SW1417 [SW-1417], and DLD-1 cell lines, respectively.ConclusionsAfter the clinical study, silver nanoparticles containing Calendula officinalis leaf aqueous extract may be used to formulate a new chemotherapeutic drug or supplement to treat the several types of human colorectal carcinoma.

TRAP1, a novel antiapoptotic gene responsible for multidrug resistance in human colorectal carcinoma cells

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. 2541-2541
Author(s):  
M. Landriscina ◽  
E. Costantino ◽  
F. Maddalena ◽  
A. Piscazzi ◽  
A. Fersini ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Multidrug Resistance ◽  
Colorectal Carcinoma ◽  
Carcinoma Cells ◽  
Dominant Negative ◽  
Multi Drug Resistance ◽  
Colorectal Carcinomas ◽  
Osteosarcoma Cells ◽  
Tumor Cell Death ◽  
Ht 29

2541 Background: TRAP1 has been recently described as a component of a mitochondrial pathway selectively up- regulated in tumor cells which antagonizes the proapoptotic activity of cyclophilin D and is responsible for maintenance of mitochondria integrity, thus favoring cell survival. Interestingly, novel TRAP1 antagonists cause sudden collapse of mitochondrial function and selective tumor cell death, suggesting that this pathway may represent a novel molecular target to improve anticancer therapy. Furthermore, we recently observed that TRAP1 is significantly up-regulated in osteosarcoma cells adapted to growth in mild oxidizing conditions and this correlates with a phenotype more resistant to H2O2-induced DNA damage and apoptosis. This evidence prompted us to investigate the role of TRAP1 as being responsible for multi-drug resistance in human colorectal cancer. Methods and Results: The evaluation of TRAP1 expression by immunoblot and quantitative RT-PCR analysis in a series of 26 human colorectal carcinomas allowed us to observe up-regulation in 17/26 tumors. Accordingly, TRAP1 protein levels were increased in HT-29 colorectal carcinoma cells resistant to 5-fluorouracil, oxaliplatin and irinotecan. Interestingly, HT-29 colorectal carcinoma and Saos-2 osteosarcoma cells transfected with TRAP1 gene exhibited a phenotype resistant to 5-fluorouracil-, oxaliplatin- and irinotecan-induced apoptosis, evaluated by MTT incorporation analysis and annexin V staining. Consistently, the transfection of a dominant negative N-terminal deletion mutant of TRAP1 as well as the inhibition of TRAP1 activity by the TRAP1 ATPase antagonist, shepherdin, increased the sensitivity to apoptosis induced by 5-fluorouracil, oxaliplatin and irinotecan in wild type HT-29 colorectal carcinoma cells and in HT-29 cells resistant to the single agents. Conclusions: These results suggest that the increased expression of TRAP1 in human colorectal carcinomas could be part of a pro-survival pathway responsible for multidrug resistance. Thus, targeting TRAP1 may represent a novel strategy to improve the efficacy of anticancer agents in colorectal cancer. No significant financial relationships to disclose.

Lack of Cyclooxygenase-2 Activity in HT-29 Human Colorectal Carcinoma Cells

2000 ◽  
Vol 256 (2) ◽  
pp. 563-570 ◽  
Author(s):  
Linda C. Hsi ◽  
Seung Joon Baek ◽  
Thomas E. Eling
Keyword(s):  
Colorectal Carcinoma ◽  
Cyclooxygenase 2 ◽  
Carcinoma Cells ◽  
Human Colorectal Carcinoma ◽  
Ht 29
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