scholarly journals Quality Evaluation of Ayurvedic Crude DrugDaruharidra, Its Allied Species, and Commercial Samples from Herbal Drug Markets of India

2013 ◽  
Vol 2013 ◽  
pp. 1-14 ◽  
Author(s):  
Sharad Srivastava ◽  
A. K. S. Rawat
Keyword(s):  
Quality Evaluation ◽  
Quantitative Estimation ◽  
Powdered Material ◽  
Drug Markets ◽  
Total Alkaloids ◽  
Ich Guidelines ◽  
Oriental Sore ◽  
Pharmaceutical Industries ◽  
Phytochemical Studies ◽  
Important Drug

Berberis aristataknown as “Daruharidra” in Ayurveda is a versatile medicinal plant used singly or in combination with other medicinal plants for treating a variety of ailments like jaundice, enlargement of spleen, leprosy, rheumatism, fever, morning/evening sickness, snakebite, and so forth. A major bioactive marker of this genus is an alkaloid berberine, which is known for its activity against cholera, acute diarrhea, amoebiasis, and latent malaria and for the treatment of oriental sore caused byLeishmania tropica. Although the roots ofB. aristataare considered as the official drug (Ayurvedic Pharmacopoeia of India), the study revealed that different species ofBerberis,namely.B. asiatica,B. chitria,andB. lyciumare also used under the name ofDaruharidrain different parts of the country. Detailed physicochemical and phytochemical studies of subjects like total ash, acid insoluble ash, tannins, and total alkaloids were calculated from the shade dried powdered material according to the recommended procedures. Further, heavy metal studies and quantitative estimation of berberine through HPTLC have also been performed as per ICH guidelines. A detailed study of fourBerberisspecies, namelyB. aristata,B. asiatica,B. chitria,andB. lycium,which are implicated asDaruharidraand collected from wild and ten commercial samples procured from various important drug markets in India has been carried out, which may be useful to pharmaceutical industries for the authentication of the commercial samples and exploring the possibilities of using other species as a substitute ofB. aristata.

scholarly journals Quality assessment of brands of erythromycin stearate tablets marketed in Abuja, Nigeria

2020 ◽  
Vol 17 (1) ◽  
pp. 29-33
Author(s):  
Kudirat B. Mustapha ◽  
Marlene D. Ekpo ◽  
Isa H. Galadima ◽  
Rukaiyatu A. Kirim ◽  
Olubukola A. Odeniran ◽  
...  
Keyword(s):  
Quality Assessment ◽  
Quality Evaluation ◽  
Hardness Test ◽  
Spectrophotometric Analysis ◽  
Assessment Study ◽  
Uniformity Test ◽  
Quality Assessment Study ◽  
Percentage Weight ◽  
Pharmaceutical Industries ◽  
Erythromycin Stearate

Erythromycin is one of the most frequently prescribed antibiotics. With the increasing number of pharmaceutical industries, the possibility of  purchasing a substandard product is on the high side. This makes it necessary to subject various commercially produced brands of erythromycin to quality evaluation in order to ascertain the quality. In this quality assessment study, six (6) brands of erythromycin tablets were subjected to physicochemical assay using standard physical methods and UV/VIS spectrophotometric analysis at 480 nm. The calibration curve for reference erythromycin was linear over a concentration range of 10-100 μg/ml with a correlation coefficient (R2) of 0.999. All the brands had standard deviation of weight greater than ±5 for the weight uniformity test. 66.67% of the brands passed the test for content of active ingredient. 83.33% of the brands examined passed the hardness test. All the brands failed the friability test because the percentage weight loss are greater than 1% as specified in the official book. Keywords: Erythromycin stearate; Tablets; Brands; Pharmaceutical Industries

scholarly journals UV SPECTROPHOTOMETRIC ANALYSIS AND VALIDATION OF ACYCLOVIR IN SOLID DOSAGE FORM

2020 ◽  
pp. 100-103
Author(s):  
AKSHATA LASURE ◽  
AFAQUE ANSARI ◽  
MALLINATH KALSHETTI
Keyword(s):  
Dosage Form ◽  
Quantitative Estimation ◽  
Spectroscopic Method ◽  
Pharmaceutical Formulations ◽  
Spectrophotometric Analysis ◽  
Pharmaceutical Dosage Form ◽  
Solid Dosage ◽  
Ich Guidelines ◽  
Double Beam ◽  
Routine Assay

Objective: A new, economical, sensitive, simple, rapid UV spectrophotometric method has been developed for the estimation of Acyclovir in pure form and pharmaceutical formulation. Methods: This UV method was developed using distilled water as a solvent. In the present method, the wavelength selected for analysis was 254 nm. UV-Visible double beam spectrophotometer (Systronic 2201) was used to carry out the spectral analysis. The ICH guidelines were used to validate the method. Results: The method was validated for linearity, range, accuracy, precision, robustness, LOD and LOQ. Linearity was found in the range of 5-30µg/ml. Accuracy was performed by using a recovery study. The amount of drug recovered was found to be in the range of 100.1-100.5 %. The % RSD value was found to be less than 2. Conclusion: The proposed UV spectroscopic method was found to be accurate, precise, stable, linear, specific, and simple for quantitative estimation of acyclovir in bulk and pharmaceutical dosage form. Hence the present UV spectroscopic method is suitable for the routine assay of acyclovir in bulk and pharmaceutical formulations.

scholarly journals IN VITRO ANTI-INFLAMMATORY AND CYTOTOXICITY OF CRINUM X AMABILE GROWN IN ECUADOR

2018 ◽  
Vol 11 (10) ◽  
pp. 99
Author(s):  
Vinueza Diego ◽  
Portero Santiago ◽  
Pilco Gisela ◽  
GarcÍa Marlene ◽  
Acosta Karen ◽  
...  
Keyword(s):  
Total Alkaloid ◽  
Inflammatory Activity ◽  
Tetrazolium Salt ◽  
Phytochemical Analysis ◽  
Total Alkaloids ◽  
Methanolic Extracts ◽  
Anti Inflammatory ◽  
Alkaloid Extracts ◽  
Phytochemical Studies

Objective: The aim of this research was to assess the in vitro anti-inflammatory activity and cytotoxicity of the methanolic extract and total alkaloid extract obtained from leaves and bulbs of Crinum x amabile (CA) growing in Ecuador.Methods: Methanolic extracts of dry powered leaves and bulbs of CA obtained by cold maceration method were subjected to preliminary phytochemical screening. Total alkaloid extracts of leaves and bulbs of CA were obtained by conventional extraction of alkaloids base with an organic solvent. Furthermore, the anti-inflammatory activity and cytotoxicity of the four extracts were investigated by in vitro isolated neutrophils model using stable tetrazolium salt (WST-1).Results: Phytochemical analysis of methanolic extracts revealed the major classes of phytochemicals such as alkaloids, flavonoids, tannins, triterpenes, and steroids, but no proteins or saponins could be detected on leaves extract. Extracts obtained from bulbs both methanolic and total alkaloids of CA show an interesting anti-inflammatory activity, although it was not significant compared to the standard anti-inflammatory drug, aspirin. Cytotoxicity of bulb alkaloid extract was lower compared with all of the other extracts.Conclusion: Based on the results of this research, it could be concluded that CA is a very interesting source of natural anti-inflammatory compounds (especially alkaloids) which could be used to prevent many chronic disorders. Further, phytochemical studies are necessary to identify the chemical compounds responsible for the significant anti-inflammatory activity showed. 

scholarly journals SIMPLE ANALYTICAL METHOD FOR THE SIMULTANEOUS ESTIMATION OF HYDROCHLOROTHIAZIDE AND CANDESARTAN BY RP-HPLC

2017 ◽  
Vol 9 (6) ◽  
pp. 34
Author(s):  
Madhavi K. ◽  
Navamani M. ◽  
Prasanthi C.
Keyword(s):  
High Performance ◽  
Limit Of Detection ◽  
Quantitative Estimation ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Pharmaceutical Dosage Form ◽  
Rp Hplc ◽  
Limit Of Quantitation ◽  
Pharmaceutical Industries

Objective: To develop a simple, rapid, economic, accurate and precise reverse phase-high performance liquid chromatographic (RP-HPLC) method for the determination of hydrochlorothiazide and candesartan in the pharmaceutical dosage form and to validate as per international conference on harmonization (ICH) guidelines.Methods: The chromatographic separation was performed on Silanol BDS C18 column (250 x 4.6 mm, 5 μm), a mobile phase consisting of water (pH adjusted to 2.8 with orthophosphoric acid): acetonitrile (30:70 % v/v), with a flow rate 1 ml/min and the detection wavelength of 210 nm using photodiode array (PDA) detector.Results: The developed method resulted in elution of hydrochlorothiazide at 2.28 min and candesartan at 4.28 min. The calibration curves were linear (r2=0.999) in the concentration range of 6.25-18.75 μg/ml and 8-24 μg/ml for hydrochlorothiazide and candesartan respectively. The percentage recoveries were found to be 99.78-100.39 for hydrochlorothiazide and 99.87-100.64 for candesartan. The limit of detection (LOD) was found to be 0.410 μg/ml and 0.699 μg/ml for hydrochlorothiazide and candesartan respectively. The limit of quantitation (LOQ) was found to be 1.367 μg/ml and 2.330 μg/ml for hydrochlorothiazide and candesartan respectively.Conclusion: A simple, economic, accurate, precise, linear and rapid RP-HPLC method was developed for simultaneous quantitative estimation of hydrochlorothiazide and candesartan in bulk and pharmaceutical formulation and the method was validated as per ICH guidelines. Hence, the method holds good for the routine analysis of hydrochlorothiazide and candesartan in various pharmaceutical industries as well as in academics.

UV Spectrophotometric Analysis and Validation of Dapsone in Semisolid Dosage Form

2021 ◽  
pp. 5007-5009
Author(s):  
Gajanand Nangare ◽  
Varsha Tegeli ◽  
Suyash Ingle ◽  
Vinod Matole ◽  
Avinash Birajdar ◽  
...  
Keyword(s):  
Dosage Form ◽  
Quantitative Estimation ◽  
Spectroscopic Method ◽  
Spectrophotometric Analysis ◽  
Ich Guidelines ◽  
Double Beam ◽  
Recovery Study ◽  
Uv Visible ◽  
Routine Assay ◽  
Stable Linear

Objectives: A new, economical, sensitive, simple, rapid UV spectrophotometric method has been developed for the estimation of Dapsone in pure form and Semisolid Dosage form. Method: This UV method was developed using methanol: RO water (75:25) as a solvent. In the present method the wavelength selected for analysis was 260nm. UV-Visible double beam spectrophotometer (Systronic 2201) was used to carry out spectral analysis. The ICH guidelines were used to validate the method. Results: The method was validated for linearity, range, accuracy, precision, robustness, LOD and LOQ. Linearity was found in the range of 3-18µg/ml. Accuracy was performed by using recovery study. The amount of drug recovered was found to be in the range of 100.1-100.5 %. The %RSD value was found to be less than 2. Conclusion: The proposed UV spectroscopic method was found to be accurate, precise, stable, linear, specific, and simple for quantitative estimation of Dapsone in bulk and Semisolid dosage form. Hence the present UV spectroscopic method is suitable for routine assay of Dapsone in bulk and Semisolid dosage form.

Method Development and Validation of Evening Primrose oil and its Pharmaceutical dosage form by Simple UV- Spectrophotometric Method

2021 ◽  
pp. 133-138
Author(s):  
Shaheen Begum ◽  
Jahnavi. B ◽  
Nameera Jabeen ◽  
Umema Naaz. T ◽  
Sandhya. M
Keyword(s):  
Dosage Form ◽  
Method Development ◽  
Quantitative Estimation ◽  
Spectroscopic Method ◽  
Evening Primrose Oil ◽  
Pharmaceutical Dosage Form ◽  
Evening Primrose ◽  
Ich Guidelines ◽  
Method Development And Validation ◽  
Development And Validation

In the present work, simple and sensitive UV spectroscopic method has been developed for the quantitative estimation of Evening Primrose Oil in efem capsule. Cyclohexane was used as solvent. In the range of 100-500μg/ml, the linearity of Evening Primrose Oil shows a correlation co-efficient of 0.999. The UV detection wavelength was 233nm. The percentage RSD for precision and accuracy of the method was found to be less than 2%(LOQ) of 23μg/ml and (LOD) of 7.5μg/ml. The method was validated as per the ICH guidelines. The method was successfully applied for routine analysis of Evening Primrose Oil and in formulation dosage form.

scholarly journals Embelin: an HPTLC method for quantitative estimation in five species of genus Embelia Burm. f.

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
K. P. Rini Vijayan ◽  
A. V. Raghu
Keyword(s):  
High Performance ◽  
Quantitative Estimation ◽  
Biological Activities ◽  
Bioactive Molecules ◽  
Plant Parts ◽  
Embelia Ribes ◽  
Ich Guidelines ◽  
Medicinal Properties ◽  
Taxonomic Studies ◽  
Hptlc Method

Abstract Background The plants belonging to the genus Embelia, a significant tropical genus with many biological activities, are benefiting because of their robust medicinal properties. Embelin is one of the principal bioactive molecules responsible for the medicinal properties of the genus Embelia. The quantification of the embelin compound among different species in this genus has not yet been investigated, so still uncertain which species and which part should be accepted. The present study was intended to establish a speedy and precise high-performance thin-layer chromatographic (HPTLC) method for quantitative study of embelin in various plant parts of Embelia ribes, Embelia tsjeriam-cottam, Embelia basaal, Embelia adnata, and Embelia gardneriana. Result This research confirmed the method as per the International Conference on Harmonization (ICH) guidelines. We achieved separation on silica gel 60 F254 HPTLC plates using propanol: butanol: ammonia (7:3:7 v/v/v) as a mobile phase. Densitometry scanning performed for detection and quantification at 254 nm and 366 nm. Among the species investigated, the highest amount of embelin was found in E. ribes fruits. Conclusion Embelia ribes fruits are the best source of embelin. Embelin was first described in the endemic species, such as E. adnata and E. gardneriana. The method illustrated in this research may be applied for quantification of embelin and fingerprint analysis of other species within Embelia genus or described genera and chemo taxonomic studies of this genus.

scholarly journals Quantitative estimation of trimazolin hydrochloride in pharmaceutical preparation by RP-HPLC method

2009 ◽  
Vol 63 (2) ◽  
pp. 87-93 ◽  
Author(s):  
Ivana Savic ◽  
Goran Nikolic ◽  
Vladimir Bankovic ◽  
Ivan Savic
Keyword(s):  
Phase Composition ◽  
Quantitative Determination ◽  
Mobile Phase ◽  
Quantitative Estimation ◽  
Pharmaceutical Preparation ◽  
Hplc Method ◽  
Mobile Phase Composition ◽  
Rp Hplc ◽  
Ich Guidelines

A sensitive and selective RP-HPLC method was developed and validated for the quantitative determination of trimazolin hydrochloride in nasal drops formulations. The mobile phase composition was water-acetonitrile (50:50, v/v) and the UV detection was carried out at 270 nm. Linearity range in the concentration range of 10 to 110 ?g cm-3. The method was tested and validated for various parameters according to ICH guidelines. The detection and quantization limits were found to be 1.45 and 4.8 ?g cm-3, respectively. The results demonstrated that the procedure for estimation of trimazolin hydrochloride in nasal drops formulations was accurate, precise and reproducible.

SIMULTANEOUS ESTIMATION OF MONTELUKAST SODIUM AND DESLORATADINE IN BULK AND IN TABLET FORMULATION BY UV-SPECTOPHOTOMETRY

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (03) ◽  
pp. 30-35
Author(s):  
R. R. Jain ◽  
◽  
P. O. Patil ◽  
S. B. Bari
Keyword(s):  
Quality Control ◽  
Spectrophotometric Method ◽  
Quantitative Estimation ◽  
Simple Procedure ◽  
Tablet Formulation ◽  
Simultaneous Equations ◽  
Routine Analysis ◽  
Simultaneous Estimation ◽  
Montelukast Sodium ◽  
Ich Guidelines

Novel, simple, sensitive and rapid spectrophotometric method has been developed for simultaneous estimation of montelukast sodium (MONTE) and desloratadine (DES). The method involved solving simultaneous equations based on measurement of absorbance at two wavelengths 285.6 nm and 245 nm, the λmax values of MONTE and DES, respectively. Beer’s law was obeyed in the concentration range of 4-24 µg/mL and 2– 12 µg/mL for MONTE and DES, respectively. The method was validated for accuracy, precision and recovery studies. The developed method was precise, reproducible, selective, specific and accurate for quantitative estimation of MONTE and DES, in combination. The wide linearity range, sensitivity, accuracy, and simple procedure demonstrated the method to be appropriate for routine analysis and quality control assays of their tablets. The method was validated according to the ICH guidelines.

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