scholarly journals Vasculoprotective Effects of Combined Endothelial Progenitor Cells and Mesenchymal Stem Cells in Diabetic Wound Care: Their Potential Role in Decreasing Wound-Oxidative Stress

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Supakanda Sukpat ◽  
Nipan Isarasena ◽  
Jutamas Wongphoom ◽  
Suthiluk Patumraj
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Wound Care ◽  
Control Group ◽  
Diabetic Mice ◽  
Mice Model ◽  
Endothelial Progenitor

To investigate whether the combined endothelial progenitor cells (EPCs) and mesenchymal stem cells (MSCs) could enhance angiogenesis and wound healing in diabetic mice. Balb/c nude mice were divided into five groups, including a control group, diabetic group (DM), DM injected with 1 × 106  cells MSCs, DM injected with 1 × 106  cells EPCs, and DM injected with combined 0.5 × 106  cells MSCs and 0.5 × 106  cells EPCs. After seven weeks, the mice were anesthetized, and bilateral full-thickness excision skin wounds were made on the dorsorostral back. The percentage of wound closure in DM group decreased significantly than in control and all other treated groups on day 7 and day 14 (P<0.005). On day 14, the percentage of capillary vascularity in combine-treated group was significantly higher than in DM (P<0.005). In the present study, we have demonstrated that the combined EPCs and MSCs can increase vascular endothelial growth factor (VEGF) level and angiogenesis which resulted in reduced neutrophil infiltration, decreased malondialdehyde (MDA) levels, and enhanced wound healing in diabetic mice model.

scholarly journals Experimental Study on the Effect of Allogeneic Endothelial Progenitor Cells on Wound Healing in Diabetic Mice

2021 ◽  
Vol 2021 ◽  
pp. 1-15
Author(s):  
Min Leng ◽  
Ying Peng ◽  
Manchang Pan ◽  
Hong Wang
Keyword(s):  
Bone Marrow ◽  
Wound Healing ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Healing Process ◽  
Control Group ◽  
Molecular Characteristics ◽  
Diabetic Mice ◽  
Endothelial Progenitor ◽  
Significant Difference

Endothelial progenitor cells (EPCs) are involved in the neovascularization in traumatic and ischemic sites, but EPCs are “detained” in bone marrow under diabetic conditions, which results in reduction of the number of EPCs and their biological activity in peripheral blood. Based on our previous study to mobilize autologous bone marrow EPCs by administering AMD3100+G-CSF to realize the optimal effect, our present study is aimed at exploring the effects of transplanting EPCs locally in a wound model of diabetic mice. First, we prepared and identified EPCs, and the biological functions and molecular characteristics were compared between EPCs from DB/+ and DB/DB mice. Then, we performed full-thickness skin resection in DB/DB mice and tested the effect of local transplantation of EPCs on skin wound healing. The wound healing process was recorded using digital photographs. The animals were sacrificed on postoperative days 7, 14, and 17 for histological and molecular analysis. Our results showed that DB/+ EPCs were biologically more active than those of DB/DB EPCs. When compared with the control group, local transplantation of EPCs accelerated wound healing in DB/DB mice by promoting wound granulation tissue formation, angiogenesis, and collagen fiber deposition, but there was no significant difference in wound healing between DB/+ EPCs and DB/DB EPCs transplanted into the wound. Furthermore, local transplantation of EPCs promoted the expression of SDF-1, CXCR4, and VEGF. We speculated that EPC transplantation may promote wound healing through the SDF-1/CXCR4 axis. This point is worth exploring further. Present data are of considerable significance because they raise the possibility of promoting wound healing by isolating autologous EPCs from the patient, which provides a new approach for the clinical treatment of diabetic wounds in the future.

Experimental Study of Anti-Interventional Endothelial Progenitor Cells Combined with Mesenchymal Stem Cells in Large Femoral Defects

2019 ◽  
Vol 9 (11) ◽  
pp. 1542-1549
Author(s):  
Jialian Chen ◽  
Fei Hu ◽  
Xiong Peng
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Density ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Bone Repair ◽  
Combined Treatment ◽  
Control Group ◽  
Endothelial Progenitor ◽  
Alp Activity

Mesenchymal stem cells (MSCs) and vascular endothelial progenitor cells (EPCs) have the potential for self-renewal and multi-directional differentiation. Their combination therapy is beneficial for treating orthopedic diseases. However, the role of antibiotic-interventional EPCs combined with MSCs in large femoral defects remains unclear. Rabbit MSCs and EPCs were isolated and EPCs were cultured in the presence of 1% green chain double antibody. The femoral segmental bone defect model was prepared and randomly divided into control group, MSCs group, EPCs group, EPCs + MSCs group, and autologous oxygen release nano-bionic scaffolds were combined with MSCs, EPCs, EPCs + MSCs followed by analysis of osteophytes by HE staining, bone density, ALP activity, TGF-β1 secretion by ELISA, and level of VEGF, Runx2 and OC by Real time PCR. MSCs, EPCs and combined treatment for large femoral bone defects can significantly improve osteophyte growth, promote bone density, increase ALP activity and TGF-β1 secretion, as well as increase VEGF, Runx2 and OC expression. Compared with control group, there was a significant difference (P < 0.05) with combined treatment group having more significant effect on bone repair. Antibioticintervention of EPCs combined with MSCs promotes osteogenic activity and bone repair ability in large femoral defects.

scholarly journals Secretome Analysis of Rabbit and Human Mesenchymal Stem and Endothelial Progenitor Cells: A Comparative Study

2021 ◽  
Vol 22 (22) ◽  
pp. 12283
Author(s):  
Jaromír Vašíček ◽  
Andrej Baláži ◽  
Mária Tirpáková ◽  
Andrea Svoradová ◽  
Ľubomír Ondruška ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Large Scale ◽  
Human Adipose Tissue ◽  
Endothelial Progenitor ◽  
Secretome Analysis ◽  
Cytokine Array ◽  
Human Cytokine

Human adipose tissue-derived mesenchymal stem cells (AT-MSCs) have been studied several years for their immunomodulatory effect through the paracrine mechanism and cytokine secretion. In combination with endothelial progenitor cells (EPCs), MSCs have great therapeutical potential for the repair of endothelium and wound healing. However, little is known about the cytokine profile of rabbit AT-MSCs or even EPCs. The aim of this study was to analyze the secretomes of these rabbit stem/progenitor cells. A large-scale human cytokine array (up to 80 cytokines) was used to identify and compare cytokines secreted into conditioned media of human and rabbit AT-MSCs as well as HUVECs and rabbit EPCs. Few cytokines were highly expressed by human AT-MSCs (TIMP-2, TIMP-1), HUVECs (MCP-1, TIMP-2, GRO, Angiogenin, IL-8, TIMP-1), or by rabbit EPCs (TIMP-2). Several cytokines have moderate expression by human (MCP-1, GRO, Angiogenin, TGF-β 2, IL-8, LIF, IL-6, Osteopontin, Osteoprotegerin) and rabbit AT-MSCs (TIMP-2, TGF-β 2, LIF, Osteopontin, IL-8, IL-5, IL-3) or by HUVECs (IL-6, MIF, TGF-β 2, GCP-2, IGFBP-2, Osteoprotegerin, EGF, LIF, PDGF-BB, MCP-3, Osteopontin, Leptin, IL-5, ENA-78, TNF- β) and rabbit EPCs (TGF-β 2, Osteopontin, GRO, LIF, IL-8, IL-5, IL-3). In conclusion, the proposed method seems to be useful for the secretome analysis of rabbit stem/progenitor cells.

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