scholarly journals Physiology of Glyphosate-Resistant and Glyphosate-Susceptible Palmer Amaranth (Amaranthus palmeri) Biotypes Collected from North Carolina

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Jared R. Whitaker ◽  
James D. Burton ◽  
Alan C. York ◽  
David L. Jordan ◽  
Aman Chandi
Keyword(s):  
North Carolina ◽  
Gene Copy Number ◽  
Palmer Amaranth ◽  
Gene Copy ◽  
Amaranthus Palmeri ◽  
Fresh Weight ◽  
Fold Level ◽  
C Distribution ◽  
Treated Leaf ◽  
Target Enzyme

Glyphosate-resistant (GR) biotypes of Palmer amaranth are now commonly found across the southern United States. Experiments were conducted to characterize physiological differences between a GR biotype and a glyphosate-susceptible (GS) biotype from North Carolina. The GR biotype had an 18-fold level of resistance based upon rates necessary to reduce shoot fresh weight 50%. Shikimate accumulated in both biotypes following glyphosate application, but greater concentrations were found in GS plants. Absorption and translocation of14C-glyphosate were studied in both biotypes with and without an overspray with commercial glyphosate potassium salt (840 g ae ha−1) immediately prior to14C-glyphosate application. Greater absorption was noted 6 h after treatment (HAT) in GS compared with GR plants, but no differences were observed at 12 to 72 HAT. Oversprayed plants absorbed 33 and 61% more14C by 48 and 72 HAT, respectively, than plants not oversprayed.14C distribution (above treated leaf, below treated leaf, roots) was similar in both biotypes. Together, these results suggest that resistance in this biotype is not due to an altered target enzyme or translocation but may be in part due to the rate of glyphosate absorption. These results also are consistent with resistance being due to increased gene copy number for the target enzyme.

Variable Inheritance of AmplifiedEPSPSGene Copies in Glyphosate-Resistant Palmer Amaranth (Amaranthus palmeri)

Weed Science ◽  
2018 ◽  
Vol 67 (2) ◽  
pp. 176-182 ◽  
Author(s):  
Darci A. Giacomini ◽  
Philip Westra ◽  
Sarah M. Ward
Keyword(s):  
Copy Number ◽  
Single Gene ◽  
Gene Copy Number ◽  
Transgressive Segregation ◽  
Palmer Amaranth ◽  
Gene Copy ◽  
Amaranthus Palmeri ◽  
Gene Copies ◽  
Central United States ◽  
Free Environment

AbstractGlyphosate-resistant (GR) Palmer amaranth (Amaranthus palmeriS. Watson) is considered one of the most troublesome weeds in the southern and central United States, but results of previous research to determine the mode of inheritance of this trait have been conflicting and inconclusive. In this study, we examined segregation patterns ofEPSPSgene-copy numbers in F1and F2generations ofA. palmeriand found no evidence of a Mendelian single-gene pattern of inheritance. Transgressive segregation for copy number was exhibited by several F1and all of the F2families, most likely the product ofEPSPScopy-number variation within each plant. This variation was confirmed by assaying gene-copy number across clonal generations and among individual shoots on the same plant, demonstrating thatEPSPSamplification levels vary significantly within a single plant. Increases and decreases in copy number occurred in a controlled, stress-free environment in the absence of glyphosate, indicating thatEPSPSgene amplification is a random and variable process within the plant. The ability ofA. palmerito gain or loseEPSPSgene copies is a valuable adaptive trait, allowing this species to respond rapidly to selection pressures and changing environments.

Interference and Control of Glyphosate-Resistant and -Susceptible Palmer Amaranth (Amaranthus palmeri) Populations under Greenhouse Conditions

Weed Science ◽  
2013 ◽  
Vol 61 (2) ◽  
pp. 259-266 ◽  
Author(s):  
Aman Chandi ◽  
David L. Jordan ◽  
Alan C. York ◽  
Susana R. Milla-Lewis ◽  
James D. Burton ◽  
...  
Keyword(s):  
North Carolina ◽  
Weight Reduction ◽  
Palmer Amaranth ◽  
Amaranthus Palmeri ◽  
Fresh Weight ◽  
Percent Reduction ◽  
Snap Bean ◽  
Crop Height ◽  
And Control ◽  
Bean Crop

Interference for 40 d after emergence (DAE) of corn, cotton, peanut, and snap bean by four glyphosate-resistant (GR) and four glyphosate-susceptible (GS) Palmer amaranth populations from Georgia and North Carolina was compared in the greenhouse. Greater interference from Palmer amaranth, measured as crop height and fresh weight reduction, was noted in cotton and peanut compared with corn or snap bean. Crop height 15 to 40 DAE was reduced similarly by GR and GS populations. Crop fresh weight, however, was reduced 25 and 19% in the presence of GS and GR populations, respectively. Measured as percent reduction in fresh weight, GR and GS populations of Palmer amaranth were controlled similarly by glufosinate, lactofen, paraquat, and trifloxysulfuron applied POST. Atrazine and dicamba controlled GR populations more effectively than GS populations.

EPSPSGene Amplification Primarily Confers Glyphosate Resistance among Arkansas Palmer amaranth (Amaranthus palmeri) Populations

Weed Science ◽  
2018 ◽  
Vol 66 (3) ◽  
pp. 293-300 ◽  
Author(s):  
Shilpa Singh ◽  
Vijay Singh ◽  
Amy Lawton-Rauh ◽  
Muthukumar V. Bagavathiannan ◽  
Nilda Roma-Burgos
Keyword(s):  
Copy Number ◽  
Gene Copy Number ◽  
Glyphosate Resistance ◽  
Resistance Mechanisms ◽  
Palmer Amaranth ◽  
Gene Copy ◽  
Amaranthus Palmeri ◽  
Resistance Level ◽  
Relative Copy Number ◽  
Gene Copies

AbstractResearch was conducted to determine whether resistance to glyphosate among Palmer amaranth (Amaranthus palmeriS. Watson) populations within the U.S. state of Arkansas was due solely to increasedEPSPSgene copy number and whether gene copy number is correlated with resistance level to glyphosate. One hundred and fifteenA. palmeriaccessions were treated with 840 g ae ha−1glyphosate. Twenty of these accessions, selected to represent a broad range of responses to glyphosate, underwent further testing. Seven of the accessions were controlled with this dose; the rest were resistant. The effective dose to cause 50% injury (ED50) for susceptible accessions ranged from 28 to 207 g ha−1. The glyphosate-resistant (GR) accessions had ED50values ranging from 494 to 1,355 g ha−1, a 3- to 48-fold resistance level compared with the susceptible standard (SS). The 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene relative copy number was determined for 20 accessions, 4 plants accession−1. Resistant plants from five GR accessions (38% of resistant plants tested) did not have increasedEPSPSgene copies. Resistant plants from the remaining eight GR accessions (62% of resistant plants tested) had 19 to 224 moreEPSPSgene copies than the SS. Among the accessions tested, injury declined 4% with every additionalEPSPScopy. ED50values were directly correlated withEPSPScopy number. The highly resistant accession MIS11-B had an ED50of 1,355 g ha−1and 150 gene copies. Partial sequences ofEPSPSfrom GR accessions withoutEPSPSamplification did not contain any of the known resistance-conferring mutations. Nearly 40% of GR accessions putatively harbor non–target site resistance mechanisms. Therefore, elevatedEPSPSgene copy number is associated with glyphosate resistance amongA. palmerifrom Arkansas.

scholarly journals Influence of Selected Fungicides on Efficacy of Clethodim and 2,4-DB

2012 ◽  
Vol 39 (2) ◽  
pp. 121-126 ◽  
Author(s):  
Gurinderbir S. Chahal ◽  
David L. Jordan ◽  
Barbara B. Shew ◽  
Rick L. Brandenburg ◽  
James D. Burton ◽  
...  
Keyword(s):  
North Carolina ◽  
Sampling Interval ◽  
Palmer Amaranth ◽  
Amaranthus Palmeri ◽  
Solution Ph ◽  
Affect Control ◽  
Solution Preparation ◽  
Positive Correlations ◽  
Sampling Times ◽  
Large Crabgrass

Abstract A range of fungicides and herbicides can be applied to control pests and optimize peanut yield. Experiments were conducted in North Carolina to define biological and physicochemical interactions when clethodim and 2,4-DB were applied alone or with selected fungicides. Pyraclostrobin consistently reduced large crabgrass [Digitaria sanguinalis (L.) Scop.] control by clethodim. Chlorothalonil and tebuconazole plus trifloxystrobin reduced large crabgrass control by clethodim in two of four experiments while prothioconazole plus tebuconazole and flutriafol did not affect control. Palmer amaranth [Amaranthus palmeri S. Wats] control by 2,4-DB was not affected by these fungicides. Although differences in spray solution pH were noted among mixtures of clethodim plus crop oil concentrate or 2,4-DB and fungicides, the range of pH was 4.40 to 4.92 and 6.72 to 7.20, respectively, across sampling times of 0, 6, 24, and 72 h after solution preparation. Permanent precipitates were formed when clethodim, crop oil concentrate, and chlorothalonil were co-applied at each sampling interval. Permanent precipitates were not observed when clethodim and crop oil concentrate were included with other fungicides or when 2,4-DB was mixed with fungicides. Significant positive correlations were noted for Palmer amaranth control by 2,4-DB and solution pH but not for clethodim and solution pH.

Susceptibility of palmer amaranth ( Amaranthus palmeri ) accessions in north carolina to Atrazine, Dicamba, S ‐metolachlor, and 2,4‐D

cftm ◽  
2021 ◽  
Author(s):  
Levi D. Moore ◽  
Katherine M. Jennings ◽  
David W. Monks ◽  
David L. Jordan ◽  
Michael D. Boyette ◽  
...  
Keyword(s):  
North Carolina ◽  
Palmer Amaranth ◽  
Amaranthus Palmeri

Critical Period for Palmer Amaranth (Amaranthus palmeri) Control in Pickling Cucumber

2018 ◽  
Vol 32 (5) ◽  
pp. 586-591
Author(s):  
Samuel J. McGowen ◽  
Katherine M. Jennings ◽  
Sushila Chaudhari ◽  
David W. Monks ◽  
Jonathan R. Schultheis ◽  
...  
Keyword(s):  
North Carolina ◽  
Critical Period ◽  
Relative Yield ◽  
Field Studies ◽  
Palmer Amaranth ◽  
Amaranthus Palmeri ◽  
Marketable Yield ◽  
Yield Data ◽  
Pickling Cucumber ◽  
Cucumber Yield

AbstractField studies were conducted in North Carolina to determine the critical period for Palmer amaranth control (CPPAC) in pickling cucumber. In removal treatments (REM), emerged Palmer amaranth were allowed to compete with cucumber for 14, 21, 28, or 35 d after sowing (DAS) in 2014 and 14, 21, 35, or 42 DAS in 2015, and cucumber was kept weed-free for the remainder of the season. In the establishment treatments (EST), cucumber was maintained free of Palmer amaranth by hand removal until 14, 21, 28, or 35 DAS in 2014 and until 14, 21, 35, or 42 DAS in 2015; after this, Palmer amaranth was allowed to establish and compete with the cucumber for the remainder of the season. The beginning and end of the CPPAC, based on 5% loss of marketable yield, was determined by fitting log-logistic and Gompertz equations to the relative yield data representing REM and EST, respectively. Season-long competition by Palmer amaranth reduced pickling cucumber yield by 45% to 98% and 88% to 98% during 2014 and 2015, respectively. When cucumber was planted on April 25, 2015, the CPPAC ranged from 570 to 1,002 heat units (HU), which corresponded to 32 to 49 DAS. However, when cucumber planting was delayed 2 to 4 wk (May 7 and May 21, 2014 and May 4, 2015), the CPPAC lasted from 100 to 918 HU (7 to 44 DAS). This research suggested that planting pickling cucumber as early as possible during the season may help to reduce competition by Palmer amaranth and delay the beginning of the CPPAC.

Susceptibility of Palmer amaranth (Amaranthus palmeri) to herbicides in accessions collected from the North Carolina Coastal Plain

Weed Science ◽  
2020 ◽  
Vol 68 (6) ◽  
pp. 582-593
Author(s):  
Denis J. Mahoney ◽  
David L. Jordan ◽  
Nilda Roma-Burgos ◽  
Katherine M. Jennings ◽  
Ramon G. Leon ◽  
...  
Keyword(s):  
North Carolina ◽  
Dose Response ◽  
Coastal Plain ◽  
Weed Management ◽  
Acetolactate Synthase ◽  
Fold Increase ◽  
Palmer Amaranth ◽  
Amaranthus Palmeri ◽  
Sites Of Action ◽  
Two Populations

AbstractPalmer amaranth (Amaranthus palmeri S. Watson) populations resistant to acetolactate synthase (ALS)-inhibiting herbicides and glyphosate are fairly common throughout the state of North Carolina (NC). This has led farm managers to rely more heavily on herbicides with other sites of action (SOA) for A. palmeri control, especially protoporphyrinogen oxidase and glutamine synthetase inhibitors. In the fall of 2016, seeds from A. palmeri populations were collected from the NC Coastal Plain, the state’s most prominent agricultural region. In separate experiments, plants with 2 to 4 leaves from the 110 populations were treated with field use rates of glyphosate, glufosinate-ammonium, fomesafen, mesotrione, or thifensulfuron-methyl. Percent visible control and survival were evaluated 3 wk after treatment. Survival frequencies were highest following glyphosate (99%) or thifensulfuron-methyl (96%) treatment. Known mutations conferring resistance to ALS inhibitors were found in populations surviving thifensulfuron-methyl application (Ala-122-Ser, Pro-197-Ser, Trp-574-Leu, and/or Ser-653-Asn), in addition to a new mutation (Ala-282-Asp) that requires further investigation. Forty-two populations had survivors after mesotrione application, with one population having 17% survival. Four populations survived fomesafen treatment, while none survived glufosinate. Dose–response studies showed an increase in fomesafen needed to kill 50% of two populations (LD50); however, these rates were far below the field use rate (less than 5 g ha−1). In two populations following mesotrione dose–response studies, a 2.4- to 3.3-fold increase was noted, with LD90 values approaching the field use rate (72.8 and 89.8 g ha−1). Screening of the progeny of individuals surviving mesotrione confirmed the presence of resistance alleles, as there were a higher number of survivors at the 1X rate compared with the parent population, confirming resistance to mesotrione. These data suggest A. palmeri resistant to chemistries other than glyphosate and thifensulfuron-methyl are present in NC, which highlights the need for weed management approaches to mitigate the evolution and spread of herbicide-resistant populations.

Interaction of Common Purslane (Portulaca oleracea) and Palmer amaranth (Amaranthus palmeri) with Sweetpotato (Ipomoea batatas) Genotypes

2020 ◽  
Author(s):  
Sushila Chaudhari ◽  
Katherine M. Jennings ◽  
David W. Monks ◽  
Lucky K. Mehra
Keyword(s):  
North Carolina ◽  
Ipomoea Batatas ◽  
Relative Yield ◽  
Palmer Amaranth ◽  
Amaranthus Palmeri ◽  
Replacement Series ◽  
Fresh Biomass ◽  
Drought Tolerant ◽  
Dry Biomass ◽  
Common Purslane

Greenhouse replacement series studies were conducted to determine the relative competitiveness of NC10-275 (unreleased, drought tolerant; upright, bushy, and vining growth with large leaves) and Covington (most commonly grown genotype in North Carolina; vining growth with smaller leaves) sweetpotato genotypes with weeds. Sweetpotato genotypes were grown with Palmer amaranth (tall growing) or common purslane (low growing) at five planting (sweetpotato:weed) proportions of 100:0, 75:25, 50:50, 25:75, and 0:100% at density of four plants pot-1. Reduction in common purslane shoot dry biomass was greater when growing with NC10-275 than when growing with Covington or alone. When growing with common purslane, shoot dry and root fresh biomass of Covington was 18 and 26% lower, respectively, than NC10-275. Relative yield (shoot dry biomass) and aggressivity index (AI) of common purslane was lower than both sweetpotato genotypes. Palmer amaranth shoot dry biomass was similar when growing alone or with Covington; whereas, it was reduced by 10% when growing with NC10-275 than alone. Palmer amaranth competition reduced shoot dry biomass and root fresh biomass of Covington by 23 and 42%, respectively, relative to NC10-275. Relative yield and AI of Palmer amaranth was greater than Covington but lower than NC10-275. This research indicates that sweetpotato genotypes differ in their ability to compete with weeds. Both sweetpotato genotypes were more competitive than common purslane, and the following species hierarchy exists: NC10-275 > Covington > common purslane. In contrast, NC10-275 was more competitive than Covington with Palmer amaranth, and the following species hierarchy exists: NC10-275 ≥ Palmer amaranth>Covington.

Critical timing of Palmer amaranth (Amaranthus palmeri) removal in sweetpotato

2020 ◽  
Vol 34 (4) ◽  
pp. 547-551 ◽  
Author(s):  
Stephen C. Smith ◽  
Katherine M. Jennings ◽  
David W. Monks ◽  
Sushila Chaudhari ◽  
Jonathan R. Schultheis ◽  
...  
Keyword(s):  
North Carolina ◽  
Logistic Model ◽  
Yield Loss ◽  
Relative Yield ◽  
Field Studies ◽  
Palmer Amaranth ◽  
Amaranthus Palmeri ◽  
Marketable Yield ◽  
Yield Data ◽  
Critical Timing

AbstractPalmer amaranth is the most common and troublesome weed in North Carolina sweetpotato. Field studies were conducted in Clinton, NC, in 2016 and 2017 to determine the critical timing of Palmer amaranth removal in ‘Covington’ sweetpotato. Palmer amaranth was grown with sweetpotato from transplanting to 2, 3, 4, 5, 6, 7, 8, and 9 wk after transplanting (WAP) and maintained weed-free for the remainder of the season. Palmer amaranth height and shoot dry biomass increased as Palmer amaranth removal was delayed. Season-long competition by Palmer amaranth interference reduced marketable yields by 85% and 95% in 2016 and 2017, respectively. Sweetpotato yield loss displayed a strong inverse linear relationship with Palmer amaranth height. A 0.6% and 0.4% decrease in yield was observed for every centimeter of Palmer amaranth growth in 2016 and 2017, respectively. The critical timing for Palmer amaranth removal, based on 5% loss of marketable yield, was determined by fitting a log-logistic model to the relative yield data and was determined to be 2 WAP. These results show that Palmer amaranth is highly competitive with sweetpotato and should be managed as early as possible in the season. The requirement of an early critical timing of weed removal to prevent yield loss emphasizes the importance of early-season scouting and Palmer amaranth removal in sweetpotato fields. Any delay in removal can result in substantial yield reductions and fewer premium quality roots.

Palmer Amaranth (Amaranthus palmeri) Management in Dicamba-Resistant Cotton

2015 ◽  
Vol 29 (4) ◽  
pp. 758-770 ◽  
Author(s):  
Charles W. Cahoon ◽  
Alan C. York ◽  
David L. Jordan ◽  
Wesley J. Everman ◽  
Richard W. Seagroves ◽  
...  
Keyword(s):  
United States ◽  
North Carolina ◽  
Field Experiment ◽  
Acetolactate Synthase ◽  
The United States ◽  
Palmer Amaranth ◽  
Amaranthus Palmeri ◽  
Cotton Yield ◽  
Herbicide Resistant

Cotton growers rely heavily upon glufosinate and various residual herbicides applied preplant, PRE, and POST to control Palmer amaranth resistant to glyphosate and acetolactate synthase-inhibiting herbicides. Recently deregulated in the United States, cotton resistant to dicamba, glufosinate, and glyphosate (B2XF cotton) offers a new platform for controlling herbicide-resistant Palmer amaranth. A field experiment was conducted in North Carolina and Georgia to determine B2XF cotton tolerance to dicamba, glufosinate, and glyphosate and to compare Palmer amaranth control by dicamba to a currently used, nondicamba program in both glufosinate- and glyphosate-based systems. Treatments consisted of glyphosate or glufosinate applied early POST (EPOST) and mid-POST (MPOST) in a factorial arrangement of treatments with seven dicamba options (no dicamba, PRE, EPOST, MPOST, PRE followed by [fb] EPOST, PRE fb MPOST, and EPOST fb MPOST) and a nondicamba standard. The nondicamba standard consisted of fomesafen PRE, pyrithiobac EPOST, and acetochlor MPOST. Dicamba caused no injury when applied PRE and only minor, transient injury when applied POST. At time of EPOST application, Palmer amaranth control by dicamba or fomesafen applied PRE, in combination with acetochlor, was similar and 13 to 17% greater than acetochlor alone. Dicamba was generally more effective on Palmer amaranth applied POST rather than PRE, and two applications were usually more effective than one. In glyphosate-based systems, greater Palmer amaranth control and cotton yield were obtained with dicamba applied EPOST, MPOST, or EPOST fb MPOST compared with the standard herbicides in North Carolina. In contrast, dicamba was no more effective than the standard herbicides in the glufosinate-based systems. In Georgia, dicamba was as effective as the standard herbicides in a glyphosate-based system only when dicamba was applied EPOST fb MPOST. In glufosinate-based systems in Georgia, dicamba was as effective as standard herbicides only when dicamba was applied twice.

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