scholarly journals Effect ofPiper betleandBrucea javanicaon the Differential Expression of Hyphal Wall Protein (HWP1) in Non-Candida albicans Candida(NCAC) Species

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Wan Himratul Aznita Wan Harun ◽  
Nur Alyaa Jamil ◽  
Nor Hazwani Jamaludin ◽  
Mohd-Al-Faisal Nordin
Keyword(s):  
Candida Albicans ◽  
Differential Expression ◽  
Piper Betle ◽  
Aqueous Extracts ◽  
Specific Primer ◽  
Chain Reaction ◽  
Transcript Levels ◽  
Brucea Javanica ◽  
Polymerase Chain ◽  
Hyphal Wall

The study aimed to identify theHWP1gene in non-Candida albicans Candidaspecies and the differential expression ofHWP1following treatment withPiper betleandBrucea javanicaaqueous extracts. All candidal suspensions were standardized to1×106 cells/mL. The suspension was incubated overnight at 37 °C (C. parapsilosis, 35°C). Candidal cells were treated with each respective extract at 1, 3, and 6 mg/mL for 24 h. The total RNA was extracted and reverse transcription-polymerase chain reaction was carried out with a specific primer ofHWP1.HWP1mRNAs were only detected inC. albicans,C. parapsilosis, andC. tropicalis. Exposing the cells to the aqueous extracts has affected the expression ofHWP1transcripts.C. albicans,C. parapsilosis, andC. tropicalishave demonstrated different intensity of mRNA. Compared toP. betle,B. javanicademonstrated a higher suppression on the transcript levels ofHWP1in all samples.HWP1was not detected inC. albicansfollowing the treatment ofB. javanicaat 1 mg/mL. In contrast,C. parapsilosisandC. tropicaliswere shown to haveHWP1regulation. However, the expression levels were reduced upon the addition of higher concentration ofB. javanicaextract.P. betleandB. javanicahave potential to be developed as oral health product.

scholarly journals Molecular characterization of Candida dubliniensis and Candida albicans in the oral cavity of drug abusers using duplex polymerase chain reaction

2018 ◽  
Vol 4 (1) ◽  
Author(s):  
Parastoo Hassani Abharian ◽  
Parvin Dehghan ◽  
Peyman Hassani Abharian ◽  
Sepideh Tolouei
Keyword(s):  
Polymerase Chain Reaction ◽  
Candida Albicans ◽  
Oral Cavity ◽  
Candida Species ◽  
Species Complex ◽  
Candida Dubliniensis ◽  
Drug Abusers ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Duplex Polymerase Chain Reaction

  Background and Purpose: Candida dubliniensis is closely related to the most pathogenic and prevalent yeast, namely C. albicans. Candida species can opportunistically overgrow in vulnerable individuals and cause a variety of diseases. The current study aimed to identify and isolate C. dubliniensis species present in the Candida albicans species complex identified in the oral cavity of drug abusers. Materials and Methods: This study was conducted on 53 strains of C. albicans species complex, isolated from the oral mucosa of drug abusers in Isfahan, Iran. DNA extraction was accomplished through boiling procedure. Duplex polymerase chain reaction (PCR) was performed to amplify ITS1-5.8S-ITS2 region using four specific primers. Fungal species were identified based on the difference in the size of the bands created in the Agarose gel. Results: Out of the 53 isolates under study, 30 (56.6%) and 14 (26.4%) samples were identified as C. albicans and C. dubliniensis, respectively. In the remaining 9 samples (17%), both types of Candida species were confirmed. Conclusion: The findings of the present study revealed the presence of a noticeable amount of C. dubliniensis in the oral cavity of drug abusers. Therefore, the probable presence of this fungus should be considered during the examination of oral infection among this group. To date, no research has directly investigated this issue in Iran.

scholarly journals Streptococcus agalactiae mastitis of bovine detection by Polymerase Chain Reaction (PCR) test in AL-Diwanyia province

2013 ◽  
Vol 12 (2) ◽  
pp. 101
Author(s):  
Gh. K. A. Al-kuzaay ◽  
Q. H. Kshash
Keyword(s):  
Polymerase Chain Reaction ◽  
Streptococcus Agalactiae ◽  
Specific Primer ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Milk Samples ◽  
Bacteriological Testing ◽  
Polymerase Chain ◽  
Pcr Test

This study was conducted for exam 348 milk samples from (clinically mastitic and other healthy cows) in many areas in AL-Diwanyia province by using CMT and bacteriological testing , which appeared that (64.9%) as percentage of mastitis ( clinically 15.9% , subclinically 84.0% ) Streptococcus agalactiae mastitis 13.2% ( 26.6% clinically , 73.3 % subclinicaly) diagnose by PCR assay by using specific primer (16SrRNA). Streptococcus agalactiae (30 isolates) after classical methods applied for streptococcus agalactiae identification (86 isolates).

Polymerase chain reaction-based detection of dermatophyte DNA with a fungus-specific primer system

Mycoses ◽  
2009 ◽  
Vol 37 (3-4) ◽  
pp. 79-84 ◽  
Author(s):  
M. Bock ◽  
M. Maiwald ◽  
R. Kappe ◽  
P. Nickel ◽  
H. Näher
Keyword(s):  
Polymerase Chain Reaction ◽  
Specific Primer ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Differential Expression of Benzophenone Synthase and Chalcone Synthase in Hypericum Sampsonii

2012 ◽  
Vol 7 (12) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Lili Huang ◽  
Hong Wang ◽  
Hechun Ye ◽  
Zhigao Du ◽  
Yansheng Zhang ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Differential Expression ◽  
Chalcone Synthase ◽  
Quantitative Polymerase Chain Reaction ◽  
Transcript Level ◽  
Reproductive Stage ◽  
Vegetative Stage ◽  
Chain Reaction ◽  
Expression Levels ◽  
Polymerase Chain

cDNAs encoding Hypericum sampsonii benzophenone synthase (HsBPS) and chalcone synthase (HsCHS) were isolated and functionally characterized. Differential expressions of HsBPS and HsCHS were monitored using quantitative polymerase chain reaction (PCR). In the vegetative stage, HsBPS was highly expressed in the roots; its transcript level was approx. 100 times higher than that of HsCHS. Relatively high transcript amounts of HsBPS were also detected in older leaves, whereas the youngest leaves contained higher transcript amounts of HsCHS. In the reproductive stage, maximum HsCHS expression was detected in flowers, the transcript level being approx. 5 times higher than that of HsBPS. The inversed situation with a 10-fold difference in the expression levels was observed with fruits. High transcript amounts for both proteins were found in roots.

Differential expression of microRNAs in porcine placentas on Days 30 and 90 of gestation

2010 ◽  
Vol 22 (8) ◽  
pp. 1175 ◽  
Author(s):  
Lijie Su ◽  
Shuhong Zhao ◽  
Mengjin Zhu ◽  
Mei Yu
Keyword(s):  
Differential Expression ◽  
Differentially Expressed ◽  
Locked Nucleic Acid ◽  
Chain Reaction ◽  
Stem Loop ◽  
Non Invasive ◽  
Differentially Expressed Mirnas ◽  
Non Coding Rnas ◽  
Polymerase Chain ◽  
And Function

The porcine placenta is classified as a non-invasive epitheliochorial type. To meet the increasing demands for nutrients by the rapidly growing conceptus and/or fetus, the placental microscopic folds undergo significant morphological and biochemical changes during two periods critical for conceptus and/or fetus, namely Days 30–40 and after Day 90 of gestation. MicroRNAs (miRNAs) are a class of small non-coding RNAs that can modulate gene activity by inhibiting the translation or regulation of mRNA degradation. In the present study, we identified 17 differentially expressed miRNAs in porcine placenta on Days 30 and 90 of gestation using a locked nucleic acid (LNA) microRNA array. Stem–loop real-time reverse transcription–polymerase chain reaction confirmed the differential expression of eight selected miRNAs (miR-24, miR-125b, miR-92b, miR-106a, miR-17, let-7i, miR-27a and miR-20). Analysis of targets and the pathways in which these miRNAs are involved revealed that the differentially expressed miRNAs target many genes that are important in various processes, including cell growth, trophoblast differentiation, angiogenesis and formation and maintenance of adherens junctions. The results of the present study suggest potential roles for these differentially expressed miRNAs in porcine placental growth and function.

Detection and Quantitation of Enterohemorrhagic Escherichia coli O157, O111, and O26 in Beef and Bovine Feces by Real-Time Polymerase Chain Reaction†

2002 ◽  
Vol 65 (9) ◽  
pp. 1371-1380 ◽  
Author(s):  
VIJAY K. SHARMA
Keyword(s):  
Real Time ◽  
Shiga Toxin ◽  
Enterohemorrhagic Escherichia Coli ◽  
Specific Primer ◽  
Chain Reaction ◽  
Pcr Assay ◽  
E Coli ◽  
Taqman Probes ◽  
Polymerase Chain ◽  
Pcr Assays

Enterohemorrhagic Escherichia coli (EHEC) O157:H7 and certain non-O157 EHEC serotypes (such as O26:H11, O26: NM, O111:H8, and O111:NM) have emerged as significant causes of human disease throughout the world. Important virulence attributes of EHEC are the intimin protein (encoded by the eae gene) and Shiga toxins 1 and 2 (encoded by the stx1 and stx2 genes, respectively). Two sets of real-time polymerase chain reaction (R-PCR) assays were developed for the simultaneous detection and quantitation of EHEC through the monitoring of the presence of the eae and stx genes, and these assays were evaluated. In the eaeR-PCR assay, three sets of primers and TaqMan probes were designed for the amplification and real-time detection of a portion of the eae gene specific to the EHEC O26, O111, and O157 serotypes. In the stxR-PCR assay, two sets of primers and TaqMan probes were used to amplify and detect the stx1 and stx2 genes. DNA prepared from 67 bacterial strains carrying known virulence markers was tested to determine the specificities of the two assays. In the eaeR-PCR assay, eaeO157- and eaeO111-specific primer-probe sets identified only EHEC O157 and O111 strains, respectively. The eaeO26-specific primer-probe set identified all EHEC O26 isolates and some Shiga toxin–negative serotypes of enteropathogenic E. coli and rabbit diarrheagenic E. coli. The stxR-PCR assay was able to identify only those strains carrying either or both of the Shiga toxin–encoding genes. The detection range of both R-PCR assays was linear over DNA concentrations corresponding to 103 to 108 CFU/ml of an EHEC strain. Both assays were able to detect and quantify very low levels (1 to 10 CFU/g of food or feces) of EHEC in feces and ground beef enriched for 16 h in a modified Trypticase soy broth. In conclusion, eae- and stx-based R-PCR assays are reliable and sensitive methods for the rapid screening and specific and quantitative detection of important serotypes of EHEC in cattle and in foods of bovine origin.

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