scholarly journals Cadmium-Induced Upregulation of Lipid Peroxidation and Reactive Oxygen Species Caused Physiological, Biochemical, and Ultrastructural Changes in Upland Cotton Seedlings

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Muhammad Daud Khan ◽  
Lei Mei ◽  
Basharat Ali ◽  
Yue Chen ◽  
Xin Cheng ◽  
...  
Keyword(s):  
Oxidative Metabolism ◽  
Ultrastructural Changes ◽  
Tolerance Index ◽  
Cd Stress ◽  
Nutrient Levels ◽  
Aerial Parts ◽  
Genetic Potential ◽  
Water Contents ◽  
Cotton Cultivar ◽  
Ultrastructural Modification

Cadmium (Cd) toxicity was investigated in cotton cultivar (ZMS-49) using physiological, ultrastructural, and biochemical parameters. Biomass-based tolerance index decreased, and water contents increased at 500 μM Cd. Photosynthetic efficiency determined by chlorophyll fluorescence and photosynthetic pigments declined under Cd stress. Cd contents were more in roots than shoots. A significant decrease in nutrient levels was found in roots and stem. A significant decrease in nutrient levels was found in roots and stems. In response to Cd stress, more MDA and ROS contents were produced in leaves than in other parts of the seedlings. Total soluble proteins were reduced in all parts except in roots at 500 μM Cd. Oxidative metabolism was higher in leaves than aerial parts of the plant. There were insignificant alterations in roots and leaves ultrastructures such as a little increase in nucleoli, vacuoles, starch granules, and plastoglobuli in Cd-imposed stressful conditions. Scanning micrographs at 500 μM Cd showed a reduced number of stomata as well as near absence of closed stomata. Cd depositions were located in cell wall, vacuoles, and intracellular spaces using TEM-EDX technology. Upregulation of oxidative metabolism, less ultrastructural modification, and Cd deposition in dead parts of cells show that ZMS-49 has genetic potential to resist Cd stress, which need to be explored.

Effects of Cadmium Stress on Alternative Oxidase and Photosystem II in Three Wheat Cultivars

2010 ◽  
Vol 65 (1-2) ◽  
pp. 87-94 ◽  
Author(s):  
Yong-Ping Duan ◽  
Shu Yuan ◽  
Shi-Hua Tu ◽  
Wen-Qiang Feng ◽  
Fei Xu ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Alternative Oxidase ◽  
Thiobarbituric Acid ◽  
Cadmium Stress ◽  
Wheat Cultivars ◽  
Cd Stress ◽  
Western Blots ◽  
Chlorophyll Contents ◽  
Water Contents ◽  
Cyanide Resistant Respiration

The effects of Cd stress (200 μmol/L, 8 days) on respiration and photosynthesis of three wheat cultivars were investigated: Chuanyu 12 (CY12), Chuanmai 42 (CM42), and Chuanmai 47 (CM47). Fifteen-day-old seedlings were exposed to 200 μmol/L CdCl2 for 4 days and 8 days, respectively. The results indicated that Cd was accumulated largely in roots, but little in leaves of all three cultivars. CY12 accumulated the highest level of Cd in roots and showed the weakest resistance. On the contrary, the other two cultivars, CM42 and CM47, adapted better to Cd stress, and their thiobarbituric acid-reactive substances (TBARS) contents were lower than in CY12, but the chlorophyll contents and water contents were higher than in CY12. Additionally, Cd stress prompted the alternative oxidase (AOX) activity and upregulated the cyanide-resistant respiration in CM42 and CM47 after 8 days; no such induction was observed for CY12. The CO2 assimilation rate, leaf stomatal conductance and chlorophyll fl uorescence were inhibited by Cd stress in all cultivars, but more severe in the CY12 cultivar. Western blots indicated that the content of the photosystem II proteins LHCII and D1 decreased in CY12, but did not change in CM42 and CM47. While the content of the mitochondrial AOX protein increased markedly in CM42 and CM47, it did not in CY12. These results suggested that AOX and LHCII could be regarded as indicators of plant’s resistance to heavy metals.

scholarly journals Slow imbibition of Annona emarginata (Annonaceae) seeds: metabolic and ultrastructural evaluations

Botany ◽  
2017 ◽  
Vol 95 (11) ◽  
pp. 1033-1040
Author(s):  
Juliana Iassia Gimenez ◽  
Amanda Cristina Esteves Amaro ◽  
Silvia Rodrigues Machado ◽  
Gisela Ferreira
Keyword(s):  
Water Content ◽  
Water Uptake ◽  
Immersion Time ◽  
Membrane System ◽  
Ultrastructural Changes ◽  
Seed Damage ◽  
Time Required ◽  
Polyethylene Glycol 6000 ◽  
Phase Phase ◽  
Water Contents

Germination pattern may be affected when seeds are dispersed under flooded conditions or subjected to pre-germination treatments such as immersion in plant growth regulators and priming. We evaluated the metabolic and ultrastructural changes in the seeds of Annona emarginata (Schltdl.) H. Rainer seeds caused by immersion in water (0 MPa) during the imbibition phase (phase I). The immersion time necessary for initially dry seeds (10% water content) to reach a water content of 15%, 20%, and 35% was recorded. The seeds were also immersed in a polyethylene glycol 6000 solution (–1.2 MPa) to decrease the rate of water uptake and increase the immersion times required for the seeds to reach the selected water contents. With shorter immersion times, seed damage did not decrease germinability. Seeds immersed for 122 h sustained damage to the cell membrane system and organelles, resulting in a decreased percentage and rate of germination. Decreasing the rate of water uptake increased the time required to finish imbibition phase to 317 h and resulted in more ultrastructural damages and a decreased germination index. We conclude that in seeds with slower imbibition, longer immersion times may cause severe metabolic damage if the seeds are immersed until the end of the imbibition phase, resulting in reduced germination.

scholarly journals Physiological and growth responses of two wheat (Triticum aestivum L.) varieties inoculated with a new strain of Bacillus siamensis under Cadmium (Cd) stress

2020 ◽  
Author(s):  
Imran Khan ◽  
Huang Linkai ◽  
Samrah Afzal Awan ◽  
Abd ur Rehman ◽  
Muhammad Ali Raza ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Plant Growth ◽  
Soluble Sugars ◽  
Membrane Damage ◽  
Growth And Yield ◽  
Tolerance Index ◽  
Photosynthetic Parameters ◽  
World Population ◽  
Growth Responses ◽  
Cd Stress

Abstract Bioavailability of cadmium (Cd) metal in the soils due to scarcity of good quality water and industrial waste could be the major limiting factors negatively influencing the growth and yield of crops needs prompt solution to fulfil the requirement of food for increasing world population. In the recent time, variable range of plant growth promoting rhizobacteria (PGPR) are being used on large scale in agriculture to reduce the risk of abiotic stresses on plants and increase crop productivity. Among them, the Bacillus siamensis has a huge potential to enhance the plant tolerance against abiotic stress but limited evidences are reported about the putative role of B.s in crop plants under heavy metal stress. The current study was aimed to investigate the potential of a new metal tolerant strain of B.s on two wheat (Triticum aestivum L.) varieties (NARC-2009 and NARC-2011) grown in Cd contaminated soil at different treatments i.e Cd (0, 20, 30 and 50 ppm) and Cd (0, 20, 30 and 50 ppm) + B.s. Our results depicted that Cd stress decreased the wheat growth related attributes, biomass, and photosynthetic parameters (Chlorophyll a, b and a + b) which increased in both wheat varieties upon inoculation with B.s. Moreover, Cd stress caused significant membrane damage and negatively affected the water content, water potential, and osmotic potential of leaf. However, PGPR considerably increased the soluble sugars to reduce the Cd toxicity. Overall, the plants inoculated with B.s enhanced their tolerance index of root and shoot and found better in NARC-2009 than NARC-2011. Therefore, microorganisms efficiently increase the plant growth by reducing the metal toxicity.

Investigation into the relationship among Cd bioaccumulation, nutrient composition, ultrastructural changes and antioxidative metabolism in lettuce genotypes under Cd stress

2019 ◽  
Vol 170 ◽  
pp. 578-589 ◽  
Author(s):  
José Lavres ◽  
Flávio Henrique Silveira Rabêlo ◽  
Flávia Regina Capaldi ◽  
André Rodrigues dos Reis ◽  
Monica Lanzoni Rosssi ◽  
...  
Keyword(s):  
Nutrient Composition ◽  
Ultrastructural Changes ◽  
Cd Stress ◽  
Antioxidative Metabolism ◽  
The Relationship

AIR POLLUTION TOLERANCE INDEX OF VARIOUS PLANT SPECIES GROWING IN INDUSTRIAL AREA OF ORAI DISTRICT JALAUN (U.P.) INDIA

2018 ◽  
Vol 24 (1) ◽  
Author(s):  
NEEL RATAN ◽  
MILAN YADAV ◽  
U.N. SINGH
Keyword(s):  
Air Pollution ◽  
Plant Species ◽  
Industrial Area ◽  
Tolerance Index ◽  
Ficus Religiosa ◽  
Pollution Tolerance ◽  
Air Pollution Tolerance Index ◽  
Relative Water ◽  
Zizyphus Jujuba ◽  
Water Contents

Air pollution tolerance index (APTI) was calculated for various plant species growing in industrial area of Orai. The leaf samples collected from 24 tree species in the industrial area were used to determine their Air Pollution Tolerance Index (APTI) by calculating the ascorbic acid, chlorophyll, pH and relative water contents. The APTI values of less than 16 are reported in 19 species and they can be used as indicators of air pollution. The species Ficus religiosa (24.92), Zizyphus jujuba (21.84), Phyllanthus emblica (17.40), Cassia fistula (17.67) and Tamarindus indica (16.96) showed their moderate response by changing their biochemical contents and were identified as moderately tolerant to air pollution.

scholarly journals Evaluation of two wild castor (Ricinus communis L.) accessions for cadmium tolerance in relation to antioxidant systems and lipid peroxidation

2021 ◽  
Author(s):  
Akwasi Yeboah ◽  
Jiannong Lu ◽  
Shuailei Gu ◽  
Haiyan Liu ◽  
Yuzhen Shi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activities ◽  
Growth Parameters ◽  
Ricinus Communis ◽  
Dry Weight ◽  
Antioxidant Systems ◽  
Control Group ◽  
Tolerance Index ◽  
Cd Stress ◽  
Mda Content

AbstractThe present study was conducted to assess the effect of toxicity of cadmium (Cd) on growth, tolerance index (TI), antioxidant activities, and malondialdehyde (MDA) content in two contrasting wild castor accessions (16-024 and S2-4) via hydroponic experiment (0 and 100 mg/L Cd). The results showed that Cd significantly reduced the growth rate, seedling height, root length, and shoot length of the castor accessions compared to the control, with the Cd effect being more severe in S2-4 than in 16-024. In addition, biomass response including the root and shoot fresh weight and root dry weight decreased in both accessions compared to the control. Compared to the control group, the shoot dry weight of accession S2-4 declined by 21.7%, whereas there was no change in 16-024, suggesting a level of tolerance in 16-024. Analysis of TI on all the growth parameters and biomass content revealed that accession 16-024 was highly tolerant to Cd stress than S2-4. The results further revealed that the expression of the antioxidant enzymes, viz., superoxide dismutase (SOD), catalase (CAT), non-enzymatic antioxidant, glutathione, and MDA content, was influenced by genotype. S2-4 exhibited a higher antioxidant activity (SOD, CAT) and lipid peroxidation activity than 16-024, indicative of oxidative damage from Cd stress.

scholarly journals Evaluation of Two Wild Castor (Ricinus Communis L.) Accessions for Cadmium Tolerance in Relation to Antioxidant Systems and Lipid Peroxidation

2021 ◽  
Author(s):  
Akwasi Yeboah ◽  
Jiannong Lu ◽  
Shuailei Gu ◽  
Liu Haiyan ◽  
Yuzhen Shi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activities ◽  
Growth Parameters ◽  
Ricinus Communis ◽  
Dry Weight ◽  
Antioxidant Systems ◽  
Tolerance Index ◽  
Cd Stress ◽  
Shoot Dry Weight ◽  
Mda Content

Abstract The present study was conducted to assess the effect of toxicity of cadmium (Cd) on growth, tolerance index (TI), antioxidant activities, and malondialdehyde (MDA) content in two contrasting wild castor accessions (16–024 and S2-4) via hydroponic experiment (0 and 100 mg/L Cd). The results showed that Cd significantly reduced the growth rate, seedling height, root length, and shoot length of the castor accessions compared to control, with the Cd effect being more severe in S2-4 than in 16–024. In addition, biomass response including the root and shoot fresh weight and root dry weight decreased in both accessions compared to control. Shoot dry weight of 16–024 increased (p > 0.05) by 2.04 % compared to S2-4 which declined by 21.07 %, suggesting a minimal effect of Cd and a level of tolerance in 16–024. Analysis of TI on all the growth parameters and biomass content revealed that accession 16–024 was highly tolerant to Cd stress than S2-4. The results further revealed that the expression of the antioxidant enzymes viz., superoxide dismutase (SOD), catalase (CAT), non-enzymatic antioxidant; glutathione, and MDA content, were influenced by genotype. S2-4 exhibited a higher antioxidant activity (SOD, CAT) and lipid peroxidation activity than 16–024, indicative of oxidative damage from Cd stress.

Fine Structural Changes in the Microvasculature of the Mouse Pinna: Aging and Radiation Injury

1974 ◽  
Vol 32 ◽  
pp. 54-55
Author(s):  
S. Phyllis Steamer ◽  
Rosemarie L. Devine
Keyword(s):  
Structural Changes ◽  
Radiation Treatment ◽  
Arterial Stenosis ◽  
Ultrastructural Changes ◽  
Vascular Effects ◽  
Microvascular Changes ◽  
Surrounding Connective Tissue ◽  
Fission Neutrons ◽  
Total Body

The importance of radiation damage to the skin and its vasculature was recognized by the early radiologists. In more recent studies, vascular effects were shown to involve the endothelium as well as the surrounding connective tissue. Microvascular changes in the mouse pinna were studied in vivo and recorded photographically over a period of 12-18 months. Radiation treatment at 110 days of age was total body exposure to either 240 rad fission neutrons or 855 rad 60Co gamma rays. After in vivo observations in control and irradiated mice, animals were sacrificed for examination of changes in vascular fine structure. Vessels were selected from regions of specific interest that had been identified on photomicrographs. Prominent ultrastructural changes can be attributed to aging as well as to radiation treatment. Of principal concern were determinations of ultrastructural changes associated with venous dilatations, segmental arterial stenosis and tortuosities of both veins and arteries, effects that had been identified on the basis of light microscopic observations. Tortuosities and irregularly dilated vein segments were related to both aging and radiation changes but arterial stenosis was observed only in irradiated animals.

Basal Lamina Formation in DMBA Induced Mammary Carcinoma

1977 ◽  
Vol 35 ◽  
pp. 534-535
Author(s):  
I. Russo ◽  
J. Saby ◽  
J. Russo
Keyword(s):  
Mammary Carcinoma ◽  
Virgin Female ◽  
Mammary Glands ◽  
Sesame Oil ◽  
Female Rats ◽  
Ultrastructural Changes ◽  
Post Inoculation ◽  
Intermediate Type ◽  
Sprague Dawley ◽  
Intercellular Spaces

It has been previously demonstrated that DMBA-induced rat mammary carcinoma originates in the terminal end bud (TEB) of the mammary gland by proliferation of intermediate type cells (1). The earliest lesion identified is the intraductal proliferation (IDP), which gives rise to intraductal carcinomas. These evolve to cribriform, papillary and comedo types (2). In the present work, we report the ultrastructural changes that take place in the IDP for the formation of a cribriform pattern.Fifty-five-day-old Sprague Dawley virgin female rats were inoculated intra- gastrically with 20 mg 7,12-dimethylbenz(a)anthracene (DMBA) in 1 ml sesame oil. Non-inoculated, age-matched females were used as controls. Mammary glands from both control and experimental rats were removed weekly from the time of inoculation until 86 days post-inoculation. The glands were fixed and processed for electron microscopy (2).The first change observed in IDP's was the widening of intercellular spaces and the secretion of an electron dense material into these spaces (Fig. 1).

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