scholarly journals The Effect of Erythropoietin on Ischemia/Reperfusion Injury after Testicular Torsion/Detorsion: A Randomized Experimental Study

ISRN Urology ◽  
2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Fahimeh Kazemi Rashed ◽  
Babollah Ghasemi ◽  
Hamid Deldade Mogaddam ◽  
Mehran Mesgari
Keyword(s):  
Testicular Torsion ◽  
Ischemia Reperfusion Injury ◽  
Protective Efficacy ◽  
Ischemia Reperfusion ◽  
Male Rats ◽  
Sham Operation ◽  
Scoring Method ◽  
Group Vi ◽  
Sham Operation Group ◽  
Group I

This study was conducted to investigate the protective effect of erythropoietin (EPO) on ischemia/reperfusion related changes after testicular torsion/detorsion. In a randomized experimental trial 30 male rats were randomly allocated into six equal groups of five rats each. Group I (orchiectomy for histopathologic examination), group II (sham operation), group III (torsion for 2 hours, and ischemia/detorsion for 24 hours, and orchiectomy); group IV (torsion for 2 hours, ischemia/detorsion for 24 hours with erythropoietin injection then orchiectomy), group V (torsion for 2 hours and detorsion and EPO injection and orchiectomy 1 week later, group VI (torsion for 2 hours/detorsion and orchiectomy 1 week later). Two groups (groups 4 and 5) received different protocols of erythropoietin administration after testicular torsion/distortion. other groups were not receiving erythropoietin. Johnsen’s spermatogenesis scoring method and Cosentino’s histologic staging method were used to assess main outcome measures of the study. After the experimentation, Johnsen’s score in EPO Groups was statistically different from the score in some groups not receiving erythropoietin. Cosentino’s score in EPO groups was statistically different from the score in all groups not receiving erythropoietin. Neovascularization, vascular necrosis, vascular congestion, edema, hemorrhage, and acute inflammation were observed in some groups. This study shows short-term protective efficacy of erythropoietin on rat testicular injury after ischemia/reperfusion.

Influences of Sufentanil Pretreatment on Ischemia-Reperfusion Injury in Rat Cardiac Apoptosis

2013 ◽  
Vol 680 ◽  
pp. 611-613
Author(s):  
Ming Gao ◽  
Guo Qing Zhao ◽  
Xiao Lu Li ◽  
Da Peng Gao
Keyword(s):  
Sham Group ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Myocardial Cell ◽  
Apoptotic Index ◽  
Sham Operation ◽  
Sham Operation Group ◽  
Myocardial Apoptosis ◽  
Sd Rats ◽  
Group I

Objective. To investigate the effects of Sufentanil preconditioning on myocardial cell apoptosis. Method. To randomly divide 40 SD rats equally into 4 groups, including sham operation group(SHAM Group), ischemia-reperfusion group(I/R Group), ischemic preconditioning group(IPC Group), Sufentanil preconditioning group(SPC Group). To calculate the numbers of myocardial apoptosis cells. Result. he apoptotic index of myocardial cell that the apoptotic index of 3 groups rises obviously compared with group S (PConclusion.Sufentanil preconditioning can protect the myocardial cell from apoptosis , therefore can protect the hearts.

scholarly journals Electroacupuncture Ameliorates Cerebral Ischemia-Reperfusion Injury by Regulation of Autophagy and Apoptosis

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Shi Shu ◽  
Chun-Ming Li ◽  
Yan-Li You ◽  
Xiao-Lu Qian ◽  
Shuang Zhou ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Cerebral Infarction ◽  
Neurological Deficit ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Beclin 1 ◽  
Male Rats ◽  
Sham Operation ◽  
Sprague Dawley ◽  
Sham Operation Group

Background. The therapeutic mechanisms of cerebral ischemia treatment by acupuncture are yet not well addressed.Objective. We investigated the effects of electroacupuncture (EA) at GV26 observing the expression of autophagy-related proteins Beclin-1 and LC3B and proportion of apoptotic cells and Bcl-2 positive cells in MCAO/R model rats.Methods. Sprague-Dawley (SD) male rats were randomly assigned to 7 groups: model groups (M6h, M24h, and M72h), EA treatment groups (T6h, T24h, and T72h), and sham operation group (S). Neurological deficit and cerebral infarction volume were measured to assess the improvement effect, while the expression of Beclin-1 and LC3B and proportion of Tunel-positive and Bcl-2 positive cells were examined to explore EA effect on autophagy and apoptosis.Results. EA significantly decreased neurological deficit scores and the volume of cerebral infarction. Beclin-1 was significantly decreased in T24h, while LC3B-II/LC3B-I ratio markedly reduced in 6th hour. EA groups markedly reduced the number of Tunel positive cells, especially in T24h. Meanwhile, the number of Bcl-2 positive cells obviously increased after EA treatment, especially in T6h and T24h.Conclusions. The alleviation of inadequate autophagy and apoptosis may be a key mechanism involved in the reflex regulation of EA at GV26 to treat cerebral ischemia.

scholarly journals THE EFFECT OF DEXAMETHASONE ON SPERMATOGONIUM AND SERTOLI CELL OF IPSILATERAL TESTIS IN UNILATERAL TESTICULAR TORSION WISTAR RAT

2018 ◽  
Vol 25 (2) ◽  
Author(s):  
Ferdyan Rachmat Efendi ◽  
Johan Renaldo ◽  
Tarmono Djojodimedjo
Keyword(s):  
Body Weight ◽  
Sertoli Cell ◽  
Sertoli Cells ◽  
Testicular Torsion ◽  
Wistar Rat ◽  
Male Rats ◽  
Sham Operation ◽  
Group I ◽  
Significant Difference ◽  
The Mean

Objective: To investigate the effect of dexamethasone on spermatogonium and sertoli cell of ipsilateral testis in unilateral testicular torsion strain wistar rat. Material & Method: Experimental study with post-test only control group design. The present  study was conducted on 30 Wistar male rats aged 10 – 12 weeks grouped into 5 groups. Group I was the normal/sham operation group (KN), group II was left testicular torsion for 4 hours group and followed  by manual detorsion  (K1), group III was left testicular torsion for 10 hours group and followed  by manual detorsion (K2),  group IV was left testicular torsion for 4 hours group and given dexamethasone 10 mg/kg body weight subcutaneously 30 minutes before manual etorsion (D1), and group V was left testicular torsion for 10 hours group and  given dexamethasone 10 mg/kg body weight subcutaneously 30 minutes before manual detorsion. All rats had left orchidectomy 4 hours after detorsion. The number of spermatogonium and sertoli cells were counted in histological seminiferous tubular testis that have obtained Haematoxylin Eosin staining. Data were analyzed by ANNOVA followed by Post Hoc Tukey for spermatogonium and Kruskal Wallis followed by Mann Whitney test for sertoli cell. Differences were considered significant at p <0.05. Results: There was significant difference in the mean number of spermatogonium between K1 & D1 group. Otherwise, there was no significant difference in the mean number of spermatogonium between K2 & D2. There was significant difference in the mean number of Sertoli cells between K1 & D1 group, likewise that between K2 & D2 group. Conclusion: These results suggest that dexamethasone has protective effect in spermatogonium and sertoli cell in testicular torsion for 4 hours.

scholarly journals Dexmedetomidine Attenuates Myocardial Ischemia-Reperfusion Injury in Diabetes Mellitus by Inhibiting Endoplasmic Reticulum Stress

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Jinjie Li ◽  
Ying Zhao ◽  
Nan Zhou ◽  
Longyun Li ◽  
Kai Li
Keyword(s):  
Endoplasmic Reticulum ◽  
Myocardial Ischemia ◽  
Endoplasmic Reticulum Stress ◽  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Sham Operation ◽  
Sham Operation Group ◽  
Myocardial Ischemia Reperfusion Injury ◽  
Myocardial Ischemia Reperfusion

Objective. With the increasing incidence of diabetes mellitus (DM) combined with myocardial ischemia, how to reduce myocardial ischemia-reperfusion injury in DM patients has become a major problem faced by clinicians. We investigated the therapeutic effects of dexmedetomidine (DEX) on myocardial ischemia-reperfusion injury in DM rats and its effect on endoplasmic reticulum stress. Methods. SD rats with SPF grade were randomly divided into 6 groups: non-DM rats were divided into the sham operation group (NDM-S group), ischemia-reperfusion group (NDM-IR group), and dexmedetomidine group (NDM-DEX group); DM rats were divided into the diabetic sham operation group (DM-S group), diabetes-reperfusion group (DM-IR group), and diabetes-dexmedetomidine (DM-DEX) group, with 10 rats in each group. Then the effects of DEX on the changes of CK-MB and cTnT levels were examined. The effects of myocardial pathological damage and myocardial infarct size were detected. The apoptosis of cardiomyocytes was detected. The apoptosis of heart tissue cells was also tested through the expressions of cleaved caspase-3, Bcl-2, and Bax proteins. The expression of endoplasmic reticulum stress-related proteins GRP78, CHOP, ERO1α, ERO1β, and PDI was examined. The hypoxia/reoxygenation (H/R) injury cell model was established, the effects of DEX, DEX+ ERS agonist on cell apoptosis was also detected. Results. The myocardial damage of DM-IR was more severe than that of NDM-IR rats. DEX could reduce the expression of CK-MB and cTnT, reduce pathological damage, and reduce scar formation and improve fibrosis. DEX can reduce the expression of GRP78, CHOP, ERO1α, ERO1β, and PDI proteins in vivo and in vitro. And the effect of DEX on cell apoptosis could be blocked by ERS agonist. Conclusion. DEX attenuates myocardial ischemia-reperfusion injury in DM rats and H/R injury cell, which is associated with the reduction of ERS-induced cardiomyocyte apoptosis.

scholarly journals Change and significance of the expression of c-kit and SCF following recovery from unilateral testicular torsion in rats

2008 ◽  
Vol 31 (3) ◽  
pp. 98 ◽  
Author(s):  
Yi Guan ◽  
XinMin Zheng ◽  
ZhiWei Yang ◽  
ShiWen Li
Keyword(s):  
Germ Cells ◽  
Testicular Torsion ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Torsion Group ◽  
Treated Group ◽  
Pcr Analysis ◽  
Control Group ◽  
Sham Operation ◽  
Expression Levels

Purpose: To investigate the change in expression levels of c-kit and SCF, and the protective effects of FSH on ischemia-reperfusion injury due to testicular torsion-detorsion. Methods: 24 adult male SD rats were divided into three groups of 8: control group, testicular torsion group and FSH-treated group. The control group was treated with sham-operation. Animals in the testicular torsion and FSH-treated groups were subjected to unilateral 720°counterclockwise testicular torsion for 2 hours and then reperfusion was allowed after detorsion. The FSH-treated group received intraperitoneal injection of FSH 15min before detorsion. Then, the rats were sacrificed and the testes were harvested. Histopathological changes were observed by light microscope, and the expression levels of c-kit, SCF in testicular tissue in the different groups were detected by Immunohistochemical assay and Quantitative Real-time RT-PCR analysis. Finally, the relative proportions of germ cells were measured by FCM. Results: c-kit and SCF were positive expressed in 52.58% and 61.16% of testicular cells of control tissues, respectively. Decreases of c-kit and SCF positive cells (15.01% and 9.18%) were found in the testicular torsion group. After being treated by FSH, the number of positive cells increased (31.25% and 20.01%). Moreover, the c-kit and SCF mRNA expression was increased dramatically (P < 0.01) in response to FSH stimulation. Furthermore, the number of haploid, diploid and tetraploid cells has also increased significantly in drug-treated testes (P < 0.01). Conclusion: The mechanism of tissue damage in the testicular torsion model, includes changes in the expression of c-kit and SCF following torsion. Also, FSH has a protective effect on germ cells after unilateral testicular torsion, which was reflected by increased c-kit and SCF levels.

scholarly journals Colchicine Attenuates Renal Ischemia-Reperfusion-Induced Liver Damage: Implication of TLR4/NF-κB, TGF-β, BAX and Bcl-2 gene expression

2021 ◽  
Author(s):  
Azza Sayed Awad ◽  
Hemat Abdel Fatah Elariny ◽  
Amany Said Sallam
Keyword(s):  
Gene Expression ◽  
Liver Damage ◽  
Transforming Growth Factor ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
B Cell Lymphoma ◽  
Sham Operation ◽  
Necrosis Factor Alpha ◽  
Sham Operation Group ◽  
Anti Inflammatory

Background: Ischemia/reperfusion injury (IRI) is typically associated with a vigorous inflammatory and oxidative stress response to hypoxia and reperfusion that disturbs the function of the organ. The remote effects of renal IRI on the liver, however, require further study. Renal damage associated with liver disease is a common clinical problem. Colchicine, a polymerization inhibitor of microtubules has been used as an anti-inflammatory and anti-fibrotic drug for liver diseases. Aims: The goal of the current study was to investigate the possible protective mechanisms of colchicine on liver injury following renal IRI. Methods: Forty rats were divided randomly into four groups; group of sham operation, group of colchicine treated, group of IRI, group of colchicine treated-IRI. Key findings: Treatment with colchicine significantly reduced hepatic toll-like receptor 4 {TLR4}, nuclear factor kappa B transcription factor {NF-κB}, myeloid differentiation factor 88 {MyD88}, and tumor necrosis factor-alpha {TNF-α} contents, down-regulated BCL2 Associated X Apoptosis Regulator {BAX} gene expression, transforming growth factor-β {TGF-β} content and upregulated hepatic B-cell lymphoma 2 {Bcl-2} gene expression as compared to the IRI group. Finally, hepatic histopathological examinations have confirmed the biochemical results. Significance: Renal IRI-induced liver damage in rats was alleviated by colchicine through its anti-inflammatory, anti-apoptotic, and anti-fibrotic actions.

scholarly journals Effects of propofol on cardiac function and miR-494 expression in rats with hepatic ischemia/reperfusion injury

2021 ◽  
Vol 49 (3) ◽  
pp. 030006052199098
Author(s):  
Jie Lv ◽  
Xiaohua Zou ◽  
Chao Yu ◽  
Wei Ou ◽  
Chengyi Sun
Keyword(s):  
Cardiac Function ◽  
Group Treatment ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Left Ventricular ◽  
Sham Operation ◽  
Myocardial Cells ◽  
Hepatic Ischemia ◽  
Sham Operation Group ◽  
Hepatic Ischemia Reperfusion

Objective This study aimed to investigate the effects of propofol on cardiac function and miR-494 expression in rats with hepatic ischemia/reperfusion (I/R) injury. Methods Forty healthy adult male Sprague-Dawley rats were allocated to the sham operation group and three hepatic I/R injury groups. The I/R injury groups included I/R injury only (I/R group), treatment with propofol (propofol group), and treatment with propofol + overexpressed miR-494 (propofol+miR-494 group). Apoptosis of myocardial cells and changes in cardiac function indices, including left ventricular end-diastolic diameter, left ventricular end-systolic diameter, and left ventricular posterior wall thickness, as well as changes in miR-494, were monitored. Results The apoptotic rate of myocardial cells in the I/R group was higher, cardiac function was deteriorated, and miR-494 levels were elevated compared with the sham group. The apoptotic rate was lower, cardiac function was improved, and miR-494 levels were suppressed in the propofol group compared with the I/R group. The apoptotic rate was higher, cardiac function was deteriorated, and miR-494 levels were elevated in the propofol+miR-494 group compared with the propofol group. Conclusion Propofol plays a vital role in preventing myocardial cell apoptosis and improvement of cardiac function by suppressing miR-494 in a hepatic I/R injury rat model.

scholarly journals Protective Role of Sulodexide on Renal Injury Induced by Limb Ischemia-Reperfusion

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Tao Yuan ◽  
Ni Yang ◽  
Wei Bi ◽  
Jinwen Zhang ◽  
Xueyan Li ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Ischemia Reperfusion ◽  
Male Rats ◽  
Inflammatory Factors ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sham Operation ◽  
Lipid Peroxidation Product ◽  
Histological Changes ◽  
Group I ◽  
Hk2 Cells

Background. Though widely known as a potent antithrombin agent with protective effects on the kidney and other remote organs, it is currently ambiguous when it comes to sulodexide’s function on ischemia-reperfusion (I/R) injury. With this research, we pursued to further explore how sulodexide exerts its influence on limb I/R injury, in which deleterious effects on the kidney were what we primarily focused on. Methods. We randomized twenty-four C57BL/6 male rats into three groups, namely, sham operation group (control group), I/R group, and sulodexide pretreatment group. Hematoxylin and eosin staining was applied for discovery of renal histological changes. Serum creatinine (Cr) and serum urea nitrogen (BUN) were measured. Apoptotic parameters were detected by the TdT-mediated dUTP Nick-End Labeling method. To what extent and levels that antiapoptotic and proapoptotic proteins were expressed could be sensitively revealed by immunohistochemistry assay. Lipid peroxidation product propylene glycol and inflammatory factors were examined by enzyme-linked immunosorbent assay. Additionally, an extracorporeal hypoxia-reoxygenation (H/R) model of human renal proximal tubule epithelial HK2 cells was established. Our targets lay in cell proliferation and apoptosis, and we used western blotting to reflect apoptosis-related gene expression. Results. The levels of serum BUN, Cr, and inflammatory factors in sulodexide-intervened rats manifested significant reduction when compared with the I/R group. Also, sulodexide could protect the kidney from histological changes and could effectively inhibit intraparenchymal apoptosis. Furthermore, adding 2 μl/mL or 5 μl/mL of sulodexide to H/R model cells in vitro gave rise to significant restoration of the degenerative proliferation capacity of the HK2 cells following H/R injury and late cellular apoptosis experienced dramatic reduction versus the H/R group. When treated with 5 μl/mL of sulodexide at a dose of 10 mg/kg, the levels of the antiapoptotic proteins were increased, while the proapoptotic proteins showed opposite trends. Notable escalation on antiapoptotic protein expression level, in contrast with the opposite trends exhibited in proapoptotic proteins, was observed with 5 μl/mL sulodexide pretreatment with the dosage being 10 mg/kg. Conclusion. Sulodexide can protect against kidney damage caused by I/R injury of the lower limbs by enhancing cell proliferation, inhibiting apoptosis, reducing inflammatory reactions, and scavenging oxygen free radicals.

scholarly journals Quantitative Evaluation of Renal Cortex Perfusion using Contrast-enhanced Ultrasound Imaging Parameters in Renal Ischemia-reperfusion Injury in Rabbits

2020 ◽  
Author(s):  
zhijian luo ◽  
yulu liu ◽  
Ziyi tang ◽  
Jialing liu ◽  
Xuemei xu ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Renal Cortex ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Sham Operation ◽  
Contrast Enhanced Ultrasound ◽  
Sham Operation Group ◽  
Injury Group ◽  
Contrast Enhanced ◽  
Post Operation

Abstract Background: Contrast-enhanced ultrasound (CEUS) can be used as a noninvasive and quantitative diagnostic method to judge the progression of renal ischemia-reperfusion injury. The aim of this study was to evaluate the blood perfusion of renal cortex during ischemia-reperfusion (I/R) injury by quantitative contrast-enhanced ultrasound (CEUS) parameters.Materials: In this experiment, 24 rabbits were randomly divided into following four groups (N=6): sham-operation group, 24-h post-operation of ischemia-reperfusion injury group (24-h I/R), 3-d post-operation of I/R injury group (3-d I/R) and 5-d post-operation of I/R injury group (5-d I/R). The I/R model was surgically established. CEUS was performed via a GE LOGIQ 9 ultrasound machine, and a time-intensity curve (TIC) in the renal cortex was generated for each group. All quantitative CEUS parameters were derived from TIC and included the following: the curve's peak ascending slope (wash-in slope [WIS]),area under the curve (AUC), time-to-peak (TTP), change in perfusion peak intensity (A), and arrival time (AT), Subsequently, we analyzed the changes in these parameters, as well as the correlation between changes in CEUS parameters and pathological parameters.Results: The values of AT, TTP, and WIS of all I/R groups significantly differed from the sham-operation group (P<0.01). However, there was make no difference in a and AUC values among the experimental groups (P > 0.05). The AT and TTP values peaked at 3 d after I/R surgery, which correlated with the most significant pathological changes at the same time point.Conclusions: Among the quantitative CEUS parameters, AT, TTP, and WIS were found to be sensitive indicators reflecting blood perfusion in renal microcirculation. Hence, these parameters may be useful for dynamically monitoring the severity of tissue damage at the early stage of I/R injury. Collectively, our findings provide compelling evidence for further clinical application of quantitative CEUS analysis.

scholarly journals Montelukast protects against testes ischemia/reperfusion injury in rats

2013 ◽  
Vol 4 (3) ◽  
pp. 174
Author(s):  
Hulya Ozturk ◽  
Hayrettin Ozturk ◽  
Kaan Gideroglu ◽  
Hakan Terzi ◽  
Guler Bugdayci
Keyword(s):  
Testicular Torsion ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Treated Group ◽  
Sham Operation ◽  
Sprague Dawley ◽  
Group 3 ◽  
Group 2 ◽  
Examen Histologique ◽  
Testicular Injury

Introduction: In this study, we investigate the effect of montelukaston histologic damage induced by testicular torsion-detorsion in rats.Methods: Twenty-one male Sprague-Dawley rats were separatedinto 3 groups, each containing 7 rats. A sham operation was performedin group 1 (control). In group 2 (ischemia-reperfusion [IR]/untreated), 1-hour detorsion of the testis was performed after6 hours of unilateral testicular torsion. In group 3 (I-R/dextroamphetamine),after performing the same surgical procedures as ingroup 2, montelukast was given intraperitoneally. In all experimentalrats, ipsilateral orchiectomies were performed for histologicalexamination and tissue malondialdehyde (MDA), glutathioneand myeloperoxidase assays.Results: Montelukast treatment significantly decreased the I-Rinducedelevation in testes tissue MDA and glutathione levelswere found to be preserved. The level of myeloperoxidase (MPO)activity was significantly increased in the testes tissue of the IR/untreated group. However, in I-R/montelukast treatment groupsignificantly decreased testes tissue MPO level. Histopathologically,the in the group 2 rats, edema, congestion, hemorrhage betweenseminiferous tubules and necrosis of the germinal cells were predominantfeatures in sections. However, most of the specimensin the montelukast treated group 3 showed grades-I and II injury.Additionally, the testicular injury score was lower in group 3 ratscompared with group 2.Conclusion: The current findings demonstrate that the montelukastdecreased the severity of testicular injury by reversing the oxidativeeffects of testes I-R.Introduction : Dans cette étude, nous examinons l’effet du montélukastsur les lésions tissulaires provoquées par torsion-détorsiontesticulaire chez le rat.Méthodologie : Vingt-et-un rats Sprague-Dawley mâles ont étérépartis en trois groupes de 7 rats. Une opération fictive a étéréalisée dans le groupe 1 (groupe témoin). Dans le groupe 2(ischémie-reperfusion / non traité), une détorsion testiculaire d’uneheure a été réalisée après 6 heures de torsion testiculaire unilatérale.Dans le groupe 3 (ischémie-reperfusion / dextroamphétamine),on a administré du montélukast par voie intrapéritonéaleaprès les mêmes interventions chirurgicales que pour le groupe 2.Chez tous les rats, une orchidectomie homolatérale a été effectuéeen vue d’un examen histologique et de mesures du malonaldéhyde,du glutathion et de la myéloperoxidase dans les tissus.Résultats : Le traitement par montélukast a significativement réduitl’élévation dans les tissus testiculaires provoquée par l’ischémiereperfusion,mais les taux de malonaldéhyde et de glutathion sontdemeurés les mêmes. Le niveau d’activité de la myéloperoxidase(MPO) a significativement augmenté dans les tissus testiculairesdu groupe ayant subi l’ischémie-reperfusion sans traitement subséquent.Cependant, dans le groupe ayant subi l’ischémiereperfusionavec traitement par montélukast, le taux de MPO dansles tissus testiculaires avait significativement diminué. Selon les examensd’histopathologie, dans le groupe 2, de l’oedème, de la congestion,des hémorragies entre les tubules séminifères et une nécrosedes cellules germinales étaient les caractéristiques prédominantesobservées dans les coupes. Par comparaison, la plupart des échantillonsdu groupe 3 (traité par montélukast) présentaient des lésionsde stade I et II. En outre, le score de lésions testiculaires était plusfaible dans le groupe 3 que dans le groupe 2.Conclusion : Les observations actuelles montrent que l’administrationde montélukast diminue la gravité des lésions testiculaires enannulant les effets oxydatifs de l’ischémie-reperfusion des testicules.

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