scholarly journals A Fibrin-Based Tissue-Engineered Renal Proximal Tubule for Bioartificial Kidney Devices: Development, Characterization and In Vitro Transport Study

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Chee Ping Ng ◽  
Yuhang Zhuang ◽  
Alex Wei Haw Lin ◽  
Jeremy Choon Meng Teo
Keyword(s):  
Proximal Tubule ◽  
Hollow Fiber ◽  
Cell Attachment ◽  
Hollow Fiber Membrane ◽  
Renal Proximal Tubule ◽  
Small Scale ◽  
Confluent Monolayer ◽  
Bioartificial Kidney ◽  
Inner Surface

A bioartificial renal proximal tubule is successfully engineered as a first step towards a bioartificial kidney for improved renal substitution therapy. To engineer the tubule, a tunable hollow fiber membrane with an exterior skin layer that provides immunoprotection for the cells from extracapillary blood flow and a coarse inner surface that facilitates a hydrogel coating for cell attachment was embedded in a “lab-on-a-chip” model for the small-scale exploratory testing under flow conditions. Fibrin was coated onto the inner surface of the hollow fiber, and human renal proximal tubule epithelial cells were then seeded. Using this model, we successfully cultured a confluent monolayer, as ascertained by immunofluorescence staining for ZO-1 tight junctions and other proximal tubule markers, scanning electron microscopy, and FITC-inulin recovery studies. Furthermore, the inulin studies, combined with the creatinine and glucose transport profiles, suggested that the confluent monolayer exhibits functional transport capabilities. The novel approaches here may eventually improve current renal substitution technology for renal failure patients.

scholarly journals Congener-Specific Polychlorinated Biphenyl–Induced Cell Death in Human Kidney Cells In Vitro: Potential Role of Caspase

2006 ◽  
Vol 25 (5) ◽  
pp. 341-347 ◽  
Author(s):  
Y. Q. Chen ◽  
S. De ◽  
S. Ghosh ◽  
S. K. Dutta
Keyword(s):  
Cell Death ◽  
Proximal Tubule ◽  
Human Kidney ◽  
Renal Proximal Tubule ◽  
Proximal Tubule Cell ◽  
Kidney Cells ◽  
Dependent Manner ◽  
Caspase Inhibitor ◽  
Pcb Congeners

Polychlorinated biphenyls (PCBs) are among the most widespread and persistent pollutants in the global environment. Coplanar and noncoplanar PCBs have been shown to cause congener-specific apoptosis mediated neurotoxicity in rats. Very few, if any, such studies have been reported on human renal cell toxicity. The authors report here caspase-dependent or caspase-independent renal toxicity, as measured by apoptotic death induced by PCBs, depending on the planarity of congeners PCB-77 (coplanar) and PCB-153 (noncoplanar) in human kidney cells (HK2) in vitro. The authors have combined morphological and biological techniques to discover the relevance of apoptosis in renal proximal tubule cell death induced by these two PCB congeners. Treatment with both PCB congeners caused accelerated apoptosis in a time-and concentration-dependent manner. Based on our findings using human kidney (HK2) cells, there was more apoptosis-mediated loss of cell viability by non– ortho-substituted PCB-77 when compared to PCB-153. A significant increase of caspase-3 expression through immunoblot studies showed the involvement of apoptosis by PCB-77 compared to none by PCB-153. The broad-spectrum caspase inhibitor z-VAD-fmk showed increased cell death when treated by PCB-153, but not by PCB-77, confirming that caspase inhibitor induced a switch in the mode of cell death. It is reasonable to assume that apoptotic cell death in the renal proximal tubule cells treated by PCBs may have both caspase-dependent and caspase-independent pathways.

In vitro effects of Npt2a inhibition in renal proximal tubule cells

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Linto Thomas ◽  
Jianxiang Xue ◽  
Jessica Dominguez Rieg ◽  
Timo Rieg
Keyword(s):  
Proximal Tubule ◽  
Renal Proximal Tubule ◽  
Proximal Tubule Cells ◽  
Tubule Cells ◽  
Renal Proximal Tubule Cells ◽  
In Vitro Effects

Hollow Fiber Immobilized L-asparaginase: In Vivo and in Vitro Immunological Studies

1983 ◽  
Vol 6 (2) ◽  
pp. 91-96 ◽  
Author(s):  
L. Callegaro ◽  
F. Assone ◽  
E. Cecconato ◽  
A. Malinverni ◽  
V. Pasteris ◽  
...  
Keyword(s):  
Hollow Fiber ◽  
Immobilized Enzyme ◽  
Hollow Fibers ◽  
Enzyme Linked Immunosorbent Assay ◽  
Related Protein ◽  
Specific Enzyme ◽  
Protein Fragments ◽  
Inner Surface

The enzyme L-asparaginase was covalently immobilized on the inner surface of the hollow fibers utilized in a commercially available dialyzer by the periodate method. After sterilization with gamma radiation the bioreactor was able to metabolize in vivo, 90 per cent of circulating asparagine in two hours. The absence in blood of asparaginase-related protein fragments, released from the hollow fiber immobilized enzyme, was monitored using a specific enzyme-linked immunosorbent assay (ELISA).

Pumping O2 with no N2: An Overview of Hollow Fiber Membrane Oxygenators with Integrated Arterial Filters

2020 ◽  
Vol 20 (1) ◽  
pp. 78-85 ◽  
Author(s):  
Anxin Liu ◽  
Zhiquan Sun ◽  
Qier Liu ◽  
Ning Zhu ◽  
Shigang Wang
Keyword(s):  
Hollow Fiber ◽  
Hollow Fiber Membrane ◽  
Experimental Studies ◽  
Circulation System ◽  
Fiber Membrane ◽  
Priming Volume ◽  
Membrane Oxygenators ◽  
Set Up

The advancement of cardiac surgery benefits from the continual technological progress of cardiopulmonary bypass (CPB). Every improvement in the CPB technology requires further clinical and laboratory tests to prove its safety and effectiveness before it can be widely used in clinical practice. In order to reduce the priming volume and eliminate a separate arterial filter in the CPB circuit, several manufacturers developed novel hollow-fiber membrane oxygenators with integrated arterial filters (IAF). Clinical and experimental studies demonstrated that an oxygenator with IAF could reduce total priming volume, blood donor exposure and gaseous microemboli delivery to the patient. It can be easily set up and managed, simplifying the CPB circuit without sacrificing safety. An oxygenator with IAF is expected to be more beneficial to the patients with low body weight and when using a minimized extracorporeal circulation system. The aim of this review manuscript was to discuss briefly the concept of integration, the current oxygenators with IAF, and the in-vitro / in-vivo performance of the oxygenators with IAF.

Abstract 503: Regulation of D5 Dopamine Receptor on Renalase Expression and Function in Rat Renal Proximal Tubule Cells

Hypertension ◽  
2012 ◽  
Vol 60 (suppl_1) ◽  
Author(s):  
Shaoxing Wang ◽  
Pedro Jose ◽  
Chunyu Zeng
Keyword(s):  
Proximal Tubule ◽  
Dopamine Receptor ◽  
Sch 23390 ◽  
Mrna Level ◽  
Inhibitory Effect ◽  
Renal Proximal Tubule ◽  
Wky Rats ◽  
Wistar Kyoto ◽  
And Function

The dopaminergic and sympathetic systems interact to regulate blood pressure. Our previous studies show the regulation of dopamine receptor on α 1 -adrenergic receptor function. Due to the regulation of renalase on sympathetic tone, we hypothesize that dopamine receptor, especially D 1 -like receptor, might regulate renalase in kidney. The effect of D 1 -like receptor on renalase expression and function was checked in immortalized renal proximal tubule (RPT) cells from Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHRs). It resulted that D 1 -like receptor agonist, fenoldopam (10 -7 -10 -5 M), increased renalase protein expression and function in WKY RPT cells, in contrast, decreased it in SHR cells. These effects were blocked by D 1 -like receptor antagonist SCH 23390 (10 -6 M). Fenoldopam increased renalase mRNA level in WKY RPT cells, but not in SHR cells. Fenoldopam increased the degradation of renalase protein in both WKY and SHR cells. However, the degradation degree was higher in SHR cells than in WKY cells. The regulation of D 1 -like receptor on renalase was mainly via D 5 receptor, because inhibition of D 5 , not D 1 receptor, by antisense blocked inhibitory effect of D 1 -like receptor on renalase in WKY cells. Moreover, inhibition of PKC, by PKC inhibitor 19-31, blocked the effect of fenoldopam on renalase expression; stimulation of PKC, by PKC agonist (PMA), inhibited renalase expression and function, indicating that PKC is involved in the process. Consistent with the in-vitro study, renalase expression was lower in kidney from SHRs than in WKY rats. It indicated that D 1 -like receptor, via D 5 receptor, regulates renalase expression and function in RPT cells, aberrant regulation of D 5 receptor on renalase might be involved in the pathogenesis of hypertension.

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