scholarly journals Mitochondrial PCR-RFLP Assay to DistinguishTriatoma brasiliensis macromelasomafromTriatoma brasiliensis brasiliensisSubspecies (Hemiptera: Reduviidae)

2013 ◽  
Vol 2013 ◽  
pp. 1-7
Author(s):  
Daniel Pagotto Vendrami ◽  
Walter Ceretti-Junior ◽  
Marcos Takashi Obara ◽  
Mauro Toledo Marrelli
Keyword(s):  
Gene Flow ◽  
Species Complex ◽  
Genetic Relationships ◽  
Northeastern Brazil ◽  
Mitochondrial Cytochrome ◽  
Ecological Requirements ◽  
Pcr Rflp ◽  
Geographical Populations ◽  
Rflp Assay ◽  
Collection Sites

Triatoma brasiliensis sensu lato(s.l.), the main vector of Chagas disease in northeastern Brazil, is a species complex comprising four species, one with two subspecies (T. brasiliensis brasiliensis,T. brasiliensis macromelasoma,T. juazeirensis,T. sherlocki, andT. melanica), and each taxon displaying distinct ecological requirements. In order to evaluate the genetic relationships among nineT. brasiliensiss.l. populations from northeastern Brazil, we analyzed their mitochondrial cytochrome c oxidase subunit 1 sequences and suggested a PCR-RFLP assay to distinguish betweenT. b. macromelasomaandT. b. brasiliensissubspecies. All the specimens were morphologically identified asT. b. brasiliensis. The resulting phylogenies identified two major clades that are congruent with the geographical populations studied. Based on collection sites and in accordance with type-location, one clade was identified as the subspeciesT. b. macromelasoma.The second clade groupedT. b. brasiliensispopulations. Restriction endonuclease sites were observed in the sequences and used in PCR-RFLP assays, producing distinct fingerprints forT. b. macromelasomaandT. b. brasiliensispopulations. The results suggest that these are different species and that gene flow occurs only amongT. b. brasiliensispopulations, possibly associated with human activity in the area.

scholarly journals PAIRWISE ASSOCIATION AS A CRITERION FOR THE SELECTION OF COLLECTION SITES OF NATURAL ENEMIES OF THE CASSAVA GREEN MITE, Mononychellus tanajoa (BONDAR)

1996 ◽  
Vol 53 (2-3) ◽  
pp. 324-331 ◽  
Author(s):  
G.S. RODRIGUES ◽  
L.C. PARAÍBA ◽  
G. J. de MORAES
Keyword(s):  
Biological Control ◽  
Natural Enemies ◽  
Interspecific Interactions ◽  
Mononychellus Tanajoa ◽  
Northeastern Brazil ◽  
Cassava Green Mite ◽  
Ecological Requirements ◽  
Green Mite ◽  
Collection Sites ◽  
Selection Of

Climatic similarity has been the primary parameter considered in the selection of sites for the collection and release of natural enemies in classical biological control programs. However, acknowledging the relevance of the composition of biological communities can be essential for improving the record of successful biocontrol projects, in relation to the proper selection of collection sites. We present in this paper an analysis of the plant and mite assemblages in cassava fields of northeastern Brazil. Such analysis is suggested as an additional criterion for the selection of collection sites of mite predators of the cassava green mite, Mononychellus tanajoa (Bondar), in an international biological control program. Contingency TABLES were built using Dice's index as an indicator of significant associations between pairs of species. This analysis enabled the identification of plant and mite species typically found together, indicating interspecific interactions or similar ecological requirements. Finally, a cluster analysis was used to group sites containing similar assemblages. These sites exhibit comparable chances of harboring a given species. Applied at the species-group level, the analysis may assist in better defining sites for the collection of natural enemies to be released in a given region, improving the chances of establishment.

PCR-RFLP Detection od PAI-2 Gene Variants: Prevelence in Ethnic Groups and Disease Relationship in patients Undergoing corony Angiography

1997 ◽  
Vol 77 (05) ◽  
pp. 0955-0958 ◽  
Author(s):  
Carole A Foy ◽  
Peter J Grant
Keyword(s):  
Gene Variants ◽  
Genotype Distribution ◽  
Pima Indians ◽  
Significant Difference ◽  
Pcr Rflp ◽  
History Of ◽  
Caucasian Patients ◽  
Clinical Conditions ◽  
Rflp Assay ◽  
Coronary Atheroma

SummaryPAI-2 is a fibrinolytic inhibitor produced predominantly by monocytes. Most PAI-2 is intracellular making study in clinical conditions difficult. Abnormalities in production may be associated with inflammation and fibrinolysis at sites of tissue damage such as the atherosclerotic plaque.PAI-2 gene variants have been described: variant A consists of Asn120, Asn404 and Ser413 and variant B consists of Asp120, Lys404 and Cys413. We designed a PCR-RFLP assay using primers spanning the region containing Asn/Lys404 and Ser/Cys413. Variant B contains an Mwol restriction site. We analysed 302 Pima Indians and 286 healthy Caucasian volunteers. To investigate relationships between genotype and vascular disease we analysed 333 Caucasian patients undergoing coronary angiography.Gene variant B was more common in the Pimas than in Caucasians (p <0.0001). There was no significant difference in genotype distribution between the volunteers and patients. In the patients there was no association between genotype and either a history of MI or extent of coronary atheroma.

PCR‐RFLP assay confirms the existence of different mitochondrial lineages of Taenia hydatigena including a possible geographically restricted group

2021 ◽  
Author(s):  
John Asekhaen Ohiolei ◽  
Hong‐Bin Yan ◽  
Li Li ◽  
Mughees Aizaz Alvi ◽  
Rosline James Muku ◽  
...  
Keyword(s):  
Taenia Hydatigena ◽  
Pcr Rflp ◽  
Restricted Group ◽  
Rflp Assay

Sex identification PCR–RFLP assay tested in eight species of Sebastes rockfish

2020 ◽  
Vol 12 (4) ◽  
pp. 541-544
Author(s):  
Felix Vaux ◽  
Hannah M. Aycock ◽  
Sandra Bohn ◽  
Leif K. Rasmuson ◽  
Kathleen G. O’Malley
Keyword(s):  
Sex Identification ◽  
Pcr Rflp ◽  
Rflp Assay

scholarly journals PCR-based genotyping assays to detect germline APC variant associated with hereditary gastrointestinal polyposis in Jack Russell terriers

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Kyoko Yoshizaki ◽  
Akihiro Hirata ◽  
Hiroyuki Matsushita ◽  
Naohito Nishii ◽  
Mifumi Kawabe ◽  
...  
Keyword(s):  
Mutant Allele ◽  
False Negative ◽  
Disease Onset ◽  
Pcr Amplification ◽  
Buccal Swab ◽  
Wild Type ◽  
Gastrointestinal Polyposis ◽  
Pcr Rflp ◽  
Formalin Fixed Paraffin Embedded ◽  
Rflp Assay

Abstract Background The prevalence of gastrointestinal (GI) neoplastic polyps in Jack Russell terriers (JRTs) has increased in Japan since the late 2000s. Recently, we demonstrated that JRTs with GI polyps harbor identical germline variant in the APC gene (c.[462_463delinsTT]) in the heterozygous state. Thus, this disease is an autosomal dominant hereditary disorder. Although the affected JRTs have distinct features, such as the development of multiple GI polyps and an early age of disease onset, genetic testing is indispensable for a definitive diagnosis. Here, polymerase chain reaction (PCR)-based assays capable of detecting germline APC variant were designed and validated using synthetic wild-type and mutant DNAs and genomic DNAs from carrier and non-carrier dogs. Result First, the PCR-restriction fragment length polymorphism (PCR-RFLP) assay was developed by taking advantage of the germline APC variant creating a new restriction site for MseI. In the PCR-RFLP assay, the 156-bp region containing the variant site was amplified by PCR and subsequently digested with MseI, yielding diagnostic 51 and 58 bp fragments from the mutant allele and allowing determination of the APC genotypes. It was possible to determine the genotypes using genomic DNA extracted from the peripheral blood, buccal swab, or formalin-fixed paraffin-embedded tissue. Next, a TaqMan duplex real-time PCR assay was developed, where a 78-bp region flanking the variant was amplified in the presence of wild-type allele- and mutant allele-specific fluorescent probes. Using blood-derived DNA, altogether 40 cycles of PCR amplification determined the APC genotypes of all examined samples by measuring the fluorescence intensities. Importantly, false-positive and false-negative errors were never detected in both assays. Conclusion In this study, we developed highly reliable genetic tests for hereditary GI polyposis in JRTs, providing accurate assessment of the presence of the causative germline APC variant. The genotyping assays could contribute to the diagnosis and prevention of hereditary GI polyposis in dogs.

scholarly journals Widespread gene flow between oceans in a pelagic seabird species complex

2017 ◽  
Vol 26 (20) ◽  
pp. 5716-5728 ◽  
Author(s):  
Katherine A. Booth Jones ◽  
Malcolm A. C. Nicoll ◽  
Claire Raisin ◽  
Deborah A. Dawson ◽  
Helen Hipperson ◽  
...  
Keyword(s):  
Gene Flow ◽  
Species Complex ◽  
Seabird Species ◽  
Pelagic Seabird

Heteroplasmy due to coexistence of mtCOI haplotypes from different lineages of theThrips tabacicryptic species group

2017 ◽  
Vol 107 (4) ◽  
pp. 534-542 ◽  
Author(s):  
S.J. Gawande ◽  
S. Anandhan ◽  
A.A. Ingle ◽  
Alana Jacobson ◽  
R. Asokan
Keyword(s):  
Flow Cytometry ◽  
Mitochondrial Dna ◽  
Real Time Pcr ◽  
Copy Number ◽  
Species Complex ◽  
Species Group ◽  
Thrips Tabaci ◽  
Mitochondrial Cytochrome ◽  
Quantitative Real Time Pcr ◽  
Putative Species

AbstractHeteroplasmy is the existence of multiple mitochondrial DNA haplotypes within the cell. Although the number of reports of heteroplasmy is increasing for arthropods, the occurrence, number of variants, and origins are not well studied. In this research, the occurrence of heteroplasmy was investigated inThrips tabaci, a putative species complex whose lineages can be distinguished by their mitochondrial DNA haplotypes. The results from this study showed that heteroplasmy was due to the occurrence of mitochondrial cytochrome oxydase I (mtCOI) haplotypes from two differentT. tabacilineages. An assay using flow cytometry and quantitative real-time PCR was then used to quantify the per cell copy number of the two mtCOI haplotypes present in individuals exhibiting heteroplasmy from nine geographically distant populations in India. All of theT. tabaciindividuals in this study were found to exhibit heteroplasmy, and in every individual the per cell copy number of mtCOI from lineage 3 comprised 75–98% of the haplotypes detected and was variable among individuals tested. There was no evidence to suggest that the presense of lineage-specific haplotypes was due to nuclear introgression; however, further studies are needed to investigate nuclear introgression and paternal leakage during rare interbreeding between individuals from lineages 2 and 3.

Habitat differentiation between estuarine and inland Hibiscus tiliaceus L. (Malvaceae) as revealed by retrotransposon-based SSAP marker

2011 ◽  
Vol 59 (6) ◽  
pp. 515 ◽  
Author(s):  
Tian Tang ◽  
Lian He ◽  
Feng Peng ◽  
Suhua Shi
Keyword(s):  
Genetic Variation ◽  
Gene Flow ◽  
Genetic Distance ◽  
Genetic Variance ◽  
Genetic Relationships ◽  
Principal Coordinate Analysis ◽  
Geographical Isolation ◽  
Tidal Zone ◽  
Ecological Scale ◽  
Habitat Differentiation

Hibiscus tiliaceus L. (Malvaceae) is a pantropical coastal tree that extends to the tidal zone. In this study, the retrotransposon sequence-specific amplified polymorphism (SSAP) technique was used in order to understand the genetic variation between four population pairs of H. tiliaceus from repeated estuarine and inland habitat contrasts in China. The estuarine populations were consistently more genetic variable compared with the inland ones, which may be attributed to extensive gene flow via water-drifted seeds and/or retrotransposon activation in stressful estuarine environments. An AMOVA revealed that 8.9% of the genetic variance could be explained by the habitat divergence within site, as compared with only 4.9% to geographical isolation between sites, which indicates significant habitat differentiation between the estuarine and inland populations. The estuarine populations were less differentiated (ΦST = 0.115) than the inland (ΦST = 0.152) implying frequent gene interchange in the former. Accordingly, the principal coordinate analysis of genetic distance between individuals revealed that genetic relationships are not fully consistent with the geographic association. These results suggest that despite substantial gene flow via sea-drifted seeds, habitat-related divergent selection could be one of the primary mechanisms that drive habitat differentiation in H. tiliaceus at a local ecological scale.

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