scholarly journals Mesothelioma in Two Nondomestic Felids: North American Cougar (Felis concolor) and Cheetah (Acinonyx jubatus)

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Amanda Whiton ◽  
Juergen Schumacher ◽  
Erika E. Evans ◽  
Janelle M. Novak ◽  
Amanda Crews ◽  
...  
Zoo Biology ◽  
1989 ◽  
Vol 8 (1) ◽  
pp. 3-16 ◽  
Author(s):  
Laurie Marker ◽  
Stephen J. O'Brien

2016 ◽  
Vol 144 (15) ◽  
pp. 3237-3243 ◽  
Author(s):  
S. MOLIA ◽  
R. W. KASTEN ◽  
M. J. STUCKEY ◽  
H. J. BOULOUIS ◽  
J. ALLEN ◽  
...  

SUMMARYBartonellae are blood- and vector-borne Gram-negative bacteria, recognized as emerging pathogens. Whole-blood samples were collected from 58 free-ranging lions (Panthera leo) in South Africa and 17 cheetahs (Acinonyx jubatus) from Namibia. Blood samples were also collected from 11 cheetahs (more than once for some of them) at the San Diego Wildlife Safari Park. Bacteria were isolated from the blood of three (5%) lions, one (6%) Namibian cheetah and eight (73%) cheetahs from California. The lionBartonellaisolates were identified asB. henselae(two isolates) andB. koehleraesubsp.koehlerae. The Namibian cheetah strain was close but distinct from isolates from North American wild felids and clustered betweenB. henselaeandB. koehlerae. It should be considered as a new subspecies ofB. koehlerae. All the Californian semi-captive cheetah isolates were different fromB. henselaeorB. koehleraesubsp.koehleraeand from the Namibian cheetah isolate. They were also distinct from the strains isolated from Californian mountain lions (Felis concolor) and clustered with strains ofB. koehleraesubsp.bothieriisolated from free-ranging bobcats (Lynx rufus) in California. Therefore, it is likely that these captive cheetahs became infected by an indigenous strain for which bobcats are the natural reservoir.


2021 ◽  
Author(s):  
Gereon Schares ◽  
Maike Joeres ◽  
Franziska Rachel ◽  
Mareen Tuschy ◽  
Gábor Á. Czirják ◽  
...  

Abstract Background: Besnoitia darlingi, B. neotomofelis and B. oryctofelisi are closely related coccidian parasites with felids as definitive hosts. These parasites use a variety of animal species as intermediate hosts. North American opossums (Didelphis virginiana), North American southern plains woodrats (Neotoma micropus) and South American domestic rabbits (Oryctolagus cuniculus) are intermediate hosts of B. darlingi, B. neotomofelis and B. oryctofelisi, respectively. Based on conserved regions in the Internal Transcribed Spacer-1 (ITS-1) sequence of the ribosomal DNA (rDNA), a real-time PCR for a sensitive detection of these Besnoitia spp. in tissues of intermediate hosts and faeces of definitive hosts has recently been established. Available sequence data suggest that species such as B. akodoni and B. jellisoni are also covered by this real-time PCR. It has been hypothesised that additional Besnoitia spp. exist worldwide, which are closely related to B. darlingi or B. darlingi-like parasites (B. neotomofelis, B. oryctofelisi, B. akodoni or B. jellisoni). Related but not closely related to these species is B. besnoiti, the cause of bovine besnoitiosis.Methods: Faecal samples from two free-ranging cheetahs (Acinonyx jubatus) from Namibia that had previously tested positive for coccidian parasites by coproscopy, were used for this study. A conventional PCR verified the presence of coccidian parasite DNA. To clarify the identity of these coccidia, the faecal DNA samples were further characterised by species-specific PCRs and Sanger sequencing.Results: One of the samples tested positive for B. darlingi or B. darlingi-like parasites by real-time PCR, while no other coccidian parasites including Toxoplasma gondii, Hammondia hammondi, H. heydorni, B. besnoiti, and Neospora caninum were detected in the two samples. The rDNA of the B. darlingi-like parasite was amplified and partially sequenced. Comparison with existing sequences in GenBank revealed a close relationship to other Besnoitia spp., but showed also clear divergences. Conclusions: Our results suggest that a so far unknown Besnoitia species exists in Namibian wildlife, which is closely related to B. darlingi, B. neotomofelis, B. oryctofelisi, B. akodoni or B. jellisoni. The cheetah appears to be the definitive host of this newly discovered parasite, while a prey species of the cheetah may act as intermediate hosts.


Zoo Biology ◽  
2017 ◽  
Vol 36 (5) ◽  
pp. 332-340
Author(s):  
Sierra J. Gillman ◽  
Karen Ziegler-Meeks ◽  
Carol Eager ◽  
Thomas A. Tenhundfeld ◽  
Wendy Shaffstall ◽  
...  

Zoo Biology ◽  
1993 ◽  
Vol 12 (1) ◽  
pp. 45-80 ◽  
Author(s):  
D. E. Wildt ◽  
J. L. Brown ◽  
M. Bush ◽  
M. A. Barone ◽  
K. A. Cooper ◽  
...  

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Gereon Schares ◽  
Maike Joeres ◽  
Franziska Rachel ◽  
Mareen Tuschy ◽  
Gábor Á. Czirják ◽  
...  

Abstract Background Besnoitia darlingi, B. neotomofelis and B. oryctofelisi are closely related coccidian parasites with felids as definitive hosts. These parasites use a variety of animal species as intermediate hosts. North American opossums (Didelphis virginiana), North American southern plains woodrats (Neotoma micropus) and South American domestic rabbits (Oryctolagus cuniculus) are intermediate hosts of B. darlingi, B. neotomofelis and B. oryctofelisi, respectively. Based on conserved regions in the internal transcribed spacer-1 (ITS1) sequence of the ribosomal DNA (rDNA), a real-time PCR for a sensitive detection of these Besnoitia spp. in tissues of intermediate hosts and faeces of definitive hosts has recently been established. Available sequence data suggest that species such as B. akodoni and B. jellisoni are also covered by this real-time PCR. It has been hypothesised that additional Besnoitia spp. exist worldwide that are closely related to B. darlingi or B. darlingi-like parasites (B. neotomofelis, B. oryctofelisi, B. akodoni or B. jellisoni). Also related, but not as closely, is B. besnoiti, the cause of bovine besnoitiosis. Methods Faecal samples from two free-ranging cheetahs (Acinonyx jubatus) from Namibia that had previously tested positive for coccidian parasites by coproscopy were used for this study. A conventional PCR verified the presence of coccidian parasite DNA. To clarify the identity of these coccidia, the faecal DNA samples were further characterised by species-specific PCRs and Sanger sequencing. Results One of the samples tested positive for B. darlingi or B. darlingi-like parasites by real-time PCR, while no other coccidian parasites, including Toxoplasma gondii, Hammondia hammondi, H. heydorni, B. besnoiti and Neospora caninum, were detected in the two samples. The rDNA of the B. darlingi-like parasite was amplified and partially sequenced. Comparison with existing sequences in GenBank revealed a close relationship to other Besnoitia spp., but also showed clear divergences. Conclusions Our results suggest that a so far unknown Besnoitia species exists in Namibian wildlife, which is closely related to B. darlingi, B. neotomofelis, B. oryctofelisi, B. akodoni or B. jellisoni. The cheetah appears to be the definitive host of this newly discovered parasite, while prey species of the cheetah may act as intermediate hosts.


2020 ◽  
Vol 29 (2) ◽  
pp. 259-264 ◽  
Author(s):  
Hasan K. Saleh ◽  
Paula Folkeard ◽  
Ewan Macpherson ◽  
Susan Scollie

Purpose The original Connected Speech Test (CST; Cox et al., 1987) is a well-regarded and often utilized speech perception test. The aim of this study was to develop a new version of the CST using a neutral North American accent and to assess the use of this updated CST on participants with normal hearing. Method A female English speaker was recruited to read the original CST passages, which were recorded as the new CST stimuli. A study was designed to assess the newly recorded CST passages' equivalence and conduct normalization. The study included 19 Western University students (11 females and eight males) with normal hearing and with English as a first language. Results Raw scores for the 48 tested passages were converted to rationalized arcsine units, and average passage scores more than 1 rationalized arcsine unit standard deviation from the mean were excluded. The internal reliability of the 32 remaining passages was assessed, and the two-way random effects intraclass correlation was .944. Conclusion The aim of our study was to create new CST stimuli with a more general North American accent in order to minimize accent effects on the speech perception scores. The study resulted in 32 passages of equivalent difficulty for listeners with normal hearing.


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