scholarly journals Organic Anion Transporter 5 Renal Expression and Urinary Excretion in Rats with Vascular Calcification

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
María Herminia Hazelhoff ◽  
Romina Paula Bulacio ◽  
Adriana Mónica Torres
Keyword(s):  
Urinary Excretion ◽  
Vascular Calcification ◽  
Western Blotting ◽  
Renal Injury ◽  
Organic Anion ◽  
Organic Anion Transporter ◽  
Protein Levels ◽  
Anion Transporter ◽  
Male Wistar Rats ◽  
Renal Expression

It has been described renal damage in rats with vascular calcification. The organic anion transporter 5 (Oat5) is only expressed in kidney, and its urinary excretion was proposed as potential early biomarker of renal injury. The aim of this study was to evaluate the Oat5 renal expression and its urinary excretion in an experimental model of vascular calcification in comparison with traditional markers of renal injury. Vascular calcification was obtained by the administration of an overdose of vitamin D3(300,000 IU/kg, b.w., i.m.) to male Wistar rats. Oat5 urinary abundance was evaluated by Western blotting. Traditional markers of renal injury, such as creatinine and urea plasma levels, urinary protein levels, and urinary alkaline phosphatase (AP) activity, were determined using commercial kits. Histology was assessed by hematoxylin/eosin staining. Oat5 renal expression was evaluated by Western blotting and by immunohistochemistry. An increased expression of Oat5 in renal homogenates, in apical membranes, and in its urinary excretion was observed in rats with vascular calcification. The traditional parameters used to evaluate renal function were not modified, with the exception of histology. It is possible to postulate the urinary excretion of Oat5 as a potential noninvasive biomarker of renal injury associated with vascular calcification.

Effect of erythropoietin on mercury-induced nephrotoxicity: Role of membrane transporters

2020 ◽  
pp. 096032712095810
Author(s):  
MH Hazelhoff ◽  
AM Torres
Keyword(s):  
Renal Function ◽  
Organic Anion ◽  
Absorption Spectrometry ◽  
Renal Tissue ◽  
Mercury Content ◽  
Organic Anion Transporter ◽  
Tubular Injury ◽  
Human Erythropoietin ◽  
Anion Transporter ◽  
Renal Expression

Mercury is a widespread pollutant. Mercuric ions uptake into tubular cells is supported by the Organic anion transporter 1 (Oat1) and 3 (Oat3) and its elimination into urine is through the Multidrug resistance-associated protein 2 (Mrp2). We investigated the effect of recombinant human erythropoietin (Epo) on renal function and on renal expression of Oat1, Oat3, and Mrp2 in a model of mercuric chloride (HgCl2)-induced renal damage. Four experimental groups of adult male Wistar rats were used: Control, Epo, HgCl2, and Epo + HgCl2. Epo (3000 IU/kg, b.w., i.p.) was administered 24 h before HgCl2 (4 mg/kg, b.w., i.p.). Experiments were performed 18 h after the HgCl2 dose. Parameters of renal function and structure were evaluated. The protein expression of Oat1, Oat3 and Mrp2 in renal tissue was assessed by immunoblotting techniques. Mercury levels were determined by cold vapor atomic absorption spectrometry. Pretreatment with Epo ameliorated the HgCl2-induced tubular injury as assessed by histopathology and urinary biomarkers. Immunoblotting showed that pretreatment with Epo regulated the renal expression of mercury transporters in a way to decrease mercury content in the kidney. Epo pretreatment ameliorates HgCl2-induced renal tubular injury by modulation of mercury transporters expression in the kidneys.

Cisplatin induces renal expression of P-glycoprotein and canalicular multispecific organic anion transporter

1999 ◽  
Vol 277 (6) ◽  
pp. F832-F840 ◽  
Author(s):  
Michel Demeule ◽  
Mathieu Brossard ◽  
Richard Béliveau
Keyword(s):  
Chemotherapeutic Agent ◽  
Organic Anion ◽  
Organic Anion Transporter ◽  
Rat Tissues ◽  
Glycoprotein P ◽  
P Glycoprotein ◽  
Anion Transporter ◽  
Renal Brush Border Membranes ◽  
Renal Expression ◽  
Renal Brush Border

The expression of two members of the ATP-binding cassette family of transport proteins, P-glycoprotein (P-gp) and the canalicular multispecific organic anion transporter (cMOAT or Mrp2), was evaluated in renal brush-border membranes (BBM) and various rat tissues after cisplatin treatment. One administration of cisplatin (5 mg/kg) increased P-gp expression by >200–300% in renal BBM and in crude membranes from liver and intestine. The increase in P-gp expression in the kidney was also detected in photolabeling experiments, suggesting the induction of functional P-gp. cMOAT expression was increased by >10-fold in renal BBM after cisplatin administration, although it had no effect on liver cMOAT expression. The increase in the levels of both proteins was maximal at 2 days after cisplatin treatment and lasted for at least 8 days. These results indicate that a single administration of cisplatin induces overexpression of P-gp and cMOAT in specific tissues. This may be of significant relevance to the design of clinical trials using cisplatin as a single chemotherapeutic agent or in combination with other drugs.

Impact of the induced organic anion transporter 1 (Oat1) renal expression by furosemide on the pharmacokinetics of organic anions

Nephrology ◽  
2017 ◽  
Vol 22 (8) ◽  
pp. 642-648 ◽  
Author(s):  
María Julia Severin ◽  
María Herminia Hazelhoff ◽  
Romina Paula Bulacio ◽  
María Eugenia Mamprin ◽  
Anabel Brandoni ◽  
...  
Keyword(s):  
Organic Anion ◽  
Organic Anions ◽  
Organic Anion Transporter ◽  
Anion Transporter ◽  
Renal Expression

scholarly journals Investigation of the Effects and Mechanisms of Dendrobium loddigesii Rolfe Extract on the Treatment of Gout

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Kai-hui Zhang ◽  
Mei-qi Wang ◽  
Lu-ling Wei ◽  
Cheng-jing Feng ◽  
Yu-si Zhang ◽  
...  
Keyword(s):  
Uric Acid ◽  
Acid Metabolism ◽  
Organic Anion ◽  
Gouty Arthritis ◽  
Organic Anion Transporter ◽  
Monosodium Urate ◽  
Acute Gouty Arthritis ◽  
Protein Levels ◽  
Anion Transporter ◽  
Uric Acid Transporters

Objective. Gout is a chronic disease that causes inflammatory arthritis, which is closely related to urate accumulation induced by a disorder of uric acid metabolism and the consequent deposition of monosodium urate crystals. Dendrobium loddigesii Rolfe is an herbal medicine that has been used in some traditional Chinese medicine formulae in the treatment of gout. This study aimed to explore and verify the antigout activity of Dendrobium loddigesii extract (DLE) on alleviating the hyperuricaemia of mice and the acute gouty arthritis of rats. Methods. An animal model of hyperuricaemia was established using potassium oxonate (PO). We analysed the expression of uric acid transporter mRNA in the kidney in the hyperuricaemic mice after treatment with DLE. Simultaneously, a monosodium urate crystal-induced acute gouty arthritis rat model was used to evaluate the effects of DLE, according to the level of ankle swelling, as well as the protein levels of inflammatory receptors and cytokines, as assayed by WB and ELISA. Results. DLE alleviated hyperuricaemia in mice and inhibited acute gouty arthritis in rats (P<0.05). Meanwhile, DLE regulated the levels of uric acid transporters mRNA transcripts, including mouse organic anion transporter 1 (mOAT1), organic anion transporter 3 (mOAT3), urate transporter 1 (mURAT1), and glucose transporter 9 (mGLUT9) in the kidney (P<0.05), suggesting that DLE promoted uric acid metabolism. Furthermore, DLE significantly suppressed the protein levels of TLRs, MyD88, and NF-κB in the ankle joint’s synovium (P<0.05), and the serum levels of IL-1β, IL-6, and TNF-α were also reduced, which demonstrated the anti-inflammatory effects of DLE. Conclusion. DLE alleviates hyperuricaemia by regulating the transcription level of uric acid transporters in the kidney. It also inhibits acute gouty arthritis by inhibiting the pathway of TLRs/MyD88/NF-κB in the ankle joint’s synovium. The findings of the present study imply that DLE alleviates gout by promoting uric acid metabolism and inhibiting inflammation related to the TLRs/MyD88/NF-κB pathway.

scholarly journals The urinary excretion of an organic anion transporter as an early biomarker of methotrexate-induced kidney injury

2016 ◽  
Vol 5 (2) ◽  
pp. 530-538 ◽  
Author(s):  
María J. Severin ◽  
Mara S. Trebucobich ◽  
Patricia Buszniez ◽  
Anabel Brandoni ◽  
Adriana M. Torres
Keyword(s):  
Early Detection ◽  
Urinary Excretion ◽  
Organic Anion ◽  
Kidney Injury ◽  
Organic Anion Transporter ◽  
Urinary Biomarker ◽  
Anion Transporter ◽  
Early Biomarker

Oat5 urinary excretion is a novel urinary biomarker for early detection of methotrexate-induced kidney injury.

Characterization of hepatobiliary organic anion transporter regulation in the Zucker rat

2004 ◽  
Vol 42 (08) ◽  
Author(s):  
A Geier ◽  
CG Dietrich ◽  
C Gartung ◽  
F Lammert ◽  
HE Wasmuth ◽  
...  
Keyword(s):  
Organic Anion ◽  
Organic Anion Transporter ◽  
Zucker Rat ◽  
Anion Transporter
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