scholarly journals Antibacterial Effect of Autologous Platelet-Rich Gel Derived from Subjects with Diabetic Dermal Ulcers In Vitro

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Lihong Chen ◽  
Chun Wang ◽  
Hengchuan Liu ◽  
Guanjian Liu ◽  
Xingwu Ran
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Effect ◽  
Control Group ◽  
Diabetic Patients ◽  
Antibacterial Effects ◽  
Significant Difference ◽  
Autologous Platelet

Background. Autologous platelet-rich gel (APG) is an effective method to improve ulcer healing. However, the mechanisms are not clear. This study aimed to investigate the antibacterial effect of APG in vitro.Methods. Platelet-rich plasma (PRP), platelet-poor plasma (PPP) and APG were prepared from whole blood of sixteen diabetic patients with dermal ulcers. Antibacterial effects againstStaphylococcus aureus,Escherichia coli, andPseudomonas aeruginosawere evaluated by bacteriostasis assay of APG, PRP, and APG-APO (APG combined with apocynin), with phosphate-buffered saline (PBS) and PPP as the control group.Results. (1) Compared to the PBS and PPP, the APG and APG-APO groups showed strong antibacterial activity againstStaphylococcus aureus. There was no significant difference(P>0.05)between APG and APG-APO. (2) Compared to PBS, APG, APG-APO, and PRP showed obvious antibacterial effects againstEscherichia coliandPseudomonas aeruginosa. No significant difference(P>0.05)was revealed among the three groups. Compared to the PPP group, they did not show antibacterial effect againstEscherichia coliandPseudomonas aeruginosa(P>0.05).Conclusions. APG has antibacterial effect againstStaphylococcus aureusmediated by platelet activation in the diabetic patients with dermal ulcer, and does not present obvious antibacterial effect againstEscherichia coliorPseudomonas aeruginosa. Combination of APG and antibiotics may have synergistic antibacterial effect.

scholarly journals Perbandingan Efek Antibakteri Madu Asli Sikabu dengan Madu Lubuk Minturun terhadap Escherichia Coli dan Staphylococcus Aureus secara In Vitro

2012 ◽  
Vol 1 (2) ◽  
Author(s):  
Yugo Berri Putra Rio ◽  
Aziz Djamal ◽  
Asterina Asterina
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Honey Bees ◽  
Antibacterial Effect ◽  
Diffusion Method ◽  
Antibacterial Effects ◽  
Traditional Medicines ◽  
The Common ◽  
And Staphylococcus Aureus

AbstrakPendahuluan: Madu digunakan sebagai agen makanan dan obat tradisional, mengandung nektar atau gulaeksudat dari tanaman yang dikumpulkan oleh lebah madu serta merupakan salah satu obat tradisional yangdigunakan oleh masyarakat. Madu diketahui memiliki kemampuan sebagai efek antibakteri, seperti Escherichia colidan Stahpylococcus aureus. Kedua bakteri ini memiliki sifat yang berbeda tetapi memilki kesamaan dari penyakityang disebabkannya. Kualitas dan jenis madu juga sesuai dengan tempat dan lokasi lebah berkembang biak.Tujuan penelitian ini adalah untuk melihat perbandingan efek antibakteri madu asli Sikabu dan Lubuk Minturunterhadap Escherichia coli dan Staphylococcus aureus secara in vitro. Metode Penelitian: Madu yang diuji untukpenelitian ini adalah madu yang berasal dari Sikabu dan Lubuk Minturun. Penelitian ini dilaksanakan pada bulanDesember 2011 sampai Mei 2012 di laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas. Jenispenelitian yang digunakan adalah eksperimental dengan menggunakan metode difusi (metode cakram) dananalitik dengan menghubungkan perbedaan yang dimiliki kedua madu sebagai efek antibakteri. Hasil Penelitian:ini menunjukkan bahwa madu asli Sikabu dan Lubuk Minturun tidak memiliki kemampuan efek antibakteri terhadapEscherichia coli, dan terdapat perbedaan efek antibakteri dari kedua madu terhadap Staphylococcus aureus.Kesimpulan: dari kedua jenis madu yang diteliti tidak ditemukan efek antibakteri terhadap Escherichia coli , namunmadu asli Sikabu memiliki efek antibakteri yang lebih baik dari pada madu Lubuk Minturun terhadapStaphylococcus aureusKata Kunci : Perbandingan Efek Antibakteri, Madu Asli Sikabu, Madu Lubuk Minturun. Escherichia coli,Staphylococcus aureusAbstractIntroduction: Honeyis used as an agent for food and traditional medicine, containing nectar or sugar exudates ofthe plant collected by honey bees, is one of the traditional medicines used by community. Honey is known to havethe ability as an antibacterial effect, such as Escherichia coli and Stahpylococcus aureus. Both of these bacteriahave different properties but have the common of the diseases it causes. The quality and type of honey is also inaccordance with the breeding places and their location. The purpose of this study was to see a comparison of theantibacterial effect of honey Sikabu and Lubuk Minturun against Escherichia coli and Staphylococcus aureus by invitro. Methods: Honey to be tested for this study were derived from Sikabu and Lubuk Minturun. This study wasconducted in December 2011 to May 2012 in the laboratory of Microbiology, Faculty of Medicine, AndalasUniversity. Type of research is experimental diffusion method (disc method) and analytical distinction owned bylinking the two of honey as an antibacterial effect. Results: These results indicate that the honey Sikabu and LubukMinturun have no antibacterial effect against Escherichia coli, both of honey have differences antibacterial effectagainst Staphylococcus aureus. Conclusion: Both types of honey from this study did not find an antibacterial effectagainst Escherichia coli, but honey Sikabu has better antibacterial against Staphylococcus aureus than honeyLubuk Minturun.Keywords: Comparisons Antibacterial Effects, Original Sikabu Honey, Honey Lubuk Minturun. Escherichia coli,Staphylococcus aureus

scholarly journals Uji Efek Antibakteri Minyak Jintan Hitam (Nigella Sativa) Dalam Kapsul yang Dijual Bebas Selama Tahun 2012 di Kota Padang Terhadap Bakteri Staphylococcus aureus dan Escherichia coli Secara In Vitro

2015 ◽  
Vol 4 (2) ◽  
Author(s):  
Gezha Utama Putra ◽  
Aziz Djamal ◽  
Machdawaty Masri
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Effect ◽  
Nigella Sativa ◽  
Antibacterial Effects ◽  
Anova Analysis ◽  
Black Cumin ◽  
Germ Free ◽  
Free Area

AbstrakJintan Hitam atau Nigella sativa diketahui memiliki kemampuan sebagai antibakteri, seperti terhadap Staphylococcus aureus dan Escherichia coli. Kedua bakteri ini memiliki sifat yang berbeda namun memiliki kesamaan dari penyakit yang disebabkannya. Tujuan penelitian ini adalah untuk membandingkan efek antibakteri produk minyak jintan hitam (Nigella sativa) dalam bentuk kapsul antara bakteri Staphylococcus aureus dan Escherechia coli secara in vitro. Penelitian ini dilakukan pada bulan Januari 2012 - Desember 2012 di Laboratorium Mikrobiologi, Fakultas Kedokteran Universitas Andalas. Penelitian ini menunjukkan bahwa semua sampel jintan hitam yang digunakan tidak memiliki efek antibakteri terhadap Escherichia coli dan semua sampel tersebut memiliki efek antibakteri yang bervariasi pada Staphylococcus aureus. Analisis Anova menunjukkan bahwa masing-masing sampel minyak Nigella sativa memberikan efek berbeda secara signifikan pada pertumbuhan Staphylococcus aureus, bahkan enam diantaranya memiliki efek yang lebih baik daripada Cepoperazon (25 mm) sebagai kontrol yang digunakan.Kata kunci: Nigella sativa, efek antibakteri, Staphylococcus aureus, Escherichia coli. AbstractBlack Cumin or Nigella sativa known have the capability as antibacterial, such as to Staphylococcus aureus and Escherichia coli. Both of these bacteria have different properties but have similarities from diseases they may cause. The objective of this study was to compare anti-bacterial effects of black cumin oil (Nigella sativa) which has been packaged in capsules towards Staphylococcus aureus and Escherichia coli in vitro The research was conducted in January 2012 to December 2012 at the Microbiology laboratory, Medicine Faculty of Andalas University. The results of this research indicate that all black cumin samples that are used did not have antibacterial effect against Escherichia coli and all samples had antibacterial effects on Staphylococcus aureus with germ-free area that size varies. Anova analysis results indicate that each of Nigella sativa gives differ effects significantly on Staphylococcus aureus growth, even six of them have better effect than Cepoperazon (25 mm) were used as controls.Keywords: Nigella sativa, antibacterial effect, Staphylococcus aureus, Escherichia coli

Activité antibactérienne de l’huile essentielle de Pelargonium x asperum et son potentiel synergique avec la nisine

2019 ◽  
Vol 17 (3) ◽  
pp. 140-148 ◽  
Author(s):  
A. Ouelhadj ◽  
L. Ait Salem ◽  
D. Djenane
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Nous Avons ◽  
Activité Antibactérienne

Ce travail vise l’étude de l’activité antibactérienne de l’huile essentielle (HE) de Pelargoniumx asperum et de la bactériocine, la nisine seul et en combinaison vis-à-vis de six bactéries dont quatre sont multirésistantes d’origine clinique. L’activité antibactérienne in vitro a été évaluée par la méthode de diffusion sur gélose. La concentration minimale inhibitrice (CMI) est aussi déterminée pour HE. Les résultats ont révélé une activité antibactérienne significative exercée par HE visà-vis de Staphylococcus aureus (ATCC 43300), Staphylococcus aureus et Escherichia coli avec des diamètres d’inhibition de 36,00 ; 22,50 et 40,00 mm, respectivement. Cependant, l’HE de Pelargonium asperum a montré une activité antibactérienne supérieure par rapport à la nisine. Les valeurs des CMI rapportées dans cette étude sont comprises entre 1,98–3,96 μl/ml. Les combinaisons réalisées entre HE et la nisine ont montré un effet additif vis-à-vis de Escherichia coli (ATCC 25922) avec (50 % HE Pelargonium asperum + 50 % nisine). Par contre, nous avons enregistré une synergie vis-à-vis de Klebsiella pneumoniae avec (75 % HE Pelargonium asperum + 25 % nisine) et contre Pseudomonas aeruginosa avec les trois combinaisons testées. Les résultats obtenus permettent de dire que l’HE de Pelargonium asperum possède une activité antibactérienne ainsi que sa combinaison avec la nisine pourrait représenter une bonne alternative pour la lutte contre l’antibiorésistance.

Exploración de la actividad antibacteriana de Metformina frente a Escherichia coli, Staphylococcus aureus y Pseudomonas aeruginosa

Bionatura ◽  
2020 ◽  
Vol 5 (4) ◽  
pp. 1335-1339
Author(s):  
Pool Marcos-Carbajal ◽  
Christian Allca-Muñoz ◽  
Ángel Urbano-Niño ◽  
Alberto Salazar-Granara
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa

El objetivo del estudio es determinar la actividad antibacteriana de Metformina frente a Escherichia coli, Staphylococcus aureus y Pseudomonas aeruginosa. Se evaluó la actividad antibacteriana mediante la técnica de Kirby Bauer. Se utilizó cepas de Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923) y Pseudomonas aeruginosa (ATCC 27853), las cuales se expusieron a Metformina en concentraciones de 250 mg y 500 mg, Ciprofloxacino (CIP) 5 µg, Imipenem (IPM) 10 µg, y Cefoxitin (FOX) 30 µg. Frente a Escherichia coli, Staphylococcus aureus y Pseudomonas aeruginosa se presentó un halo de inhibición de 6 mm. para Metformina 250 mg, 6 mm. para Metformina 500 mg, y un halo de inhibición >25 mm. con el uso de Ciprofloxacino 5 µg, Cefoxitin 30 µg, e Imipenem 10 µg respectivamente. En conclusion, In vitro Metformina a dosis de 250 y 500 mg, no presentó efecto antibacteriano frente a Escherichia coli, Staphylococcus aureus y Pseudomonas aeruginosa.

scholarly journals Synergistic antibacterial combination of Lavandula latifolia Medik. essential oil with camphor

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Nursenem Karaca ◽  
Görkem Şener ◽  
Betül Demirci ◽  
Fatih Demirci
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Antibacterial Effect ◽  
Synergistic Effects ◽  
Pharmaceutical Formulations ◽  
Human Pathogens ◽  
Broth Microdilution ◽  
Antibacterial Effects ◽  
Lavandula Latifolia

AbstractCombination of various compounds and essential oils for pharmaceutical formulations withdraw attention. In this present study, it was aimed to evaluate the in vitro potential synergistic antibacterial effect of Lavandula latifolia (spike lavender) essential oil with camphor by using the checkerboard method against the human pathogens; Staphylococcus aureus and Listeria monocytogenes. Pharmacopoeia quality L. latifolia essential oil and racemic camphor were analyzed and verified by GC-FID and GC/MS, simultaneously. In vitro antibacterial activity of essential oil and camphor (MIC range: 0.16–20 mg/mL) and standard antimicrobial clarithromycin (MIC range: 0.125–16 μg/mL) were carried out by broth microdilution against S. aureus and L. monocytogenes standard strains, respectively. Resulting antibacterial effects were evaluated for their fractional inhibitory concentrations (FICs) as antagonistic, additive and synergistic effects. The analytical results showed that the major component of essential oil was linalool (45.2%) and 1,8-cineole (25.6%). Antibacterial effects of essential oil were determined as MIC 1.25–5 mg/mL. As a result of the experiments, L. latifolia essential oil–camphor combinations were identified as “synergistic (FIC ≤ 0.5), and additive (0.5 < FIC ≤ 1)” in the respective combinations, suggesting further evaluation for formulations for potential antimicrobial applications in food and pharmaceuticals.

scholarly journals Efeito antimicrobiano do extrato de Cranberry sobre micro-organismos causadores de infecção urinária

2016 ◽  
Vol 11 (31) ◽  
pp. 113-122
Author(s):  
Carla Franco Porto Belmont Souza ◽  
Luiz Eduardo Souza da Silva Irineu ◽  
Renan Silva De Souza ◽  
Renato da Silva Teixeira ◽  
Ivina Sanches Pereira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Enterococcus Faecalis ◽  
Proteus Mirabilis ◽  
Enterococcus Faecium ◽  
Vaccinium Macrocarpon ◽  
Mueller Hinton

A resistência microbiana tem se mostrado um problema de proporções mundiais, causando estado de morbidade e mortalidade em diversos pacientes. Em vista disso, tem crescido a busca por métodos alternativos naturais de profilaxia. A investigação clínica sugere que o Extrato de Cranberry está entre as melhores propostas de prevenção natural. O Cranberry (Vaccinium macrocarpon) é um fruto que tem crescido comercialmente pelo sabor e propriedades benéficas à saúde. Dentre as formas comercializadas estão: o suco, o chá e as cápsulas contendo o extrato seco. A ação desta planta está relacionada ao tratamento de doenças do trato urinário, por possuir substâncias que inibem a adesão bacteriana ao epitélio do trato urinário, dificultando sua proliferação e reprodução. Dentre todas as infecções relacionadas à assistência a saúde, a Infecção do Trato Urinário é a mais frequentemente associada a procedimentos invasivos. Se não for tratada, pode resultar em complicações como pielonefrite aguda, bacteremia e pionefrose. Portanto, cranberry pode ser uma nova alternativa para o combate das infecções uroepiteliais, por ser um produto natural de preço acessível, e com formas de comercialização diversificada, ao contrário dos antimicrobianos convencionais, que por sua vez são caros e podem acabar causando resistência nos micro-organismos. Este trabalho teve como objetivo avaliar in vitro a atividade antimicrobiana do extrato de Cranberry, adquirido em farmácia de manipulação, sobre 8 micro-organismos isolados de infecções urinárias. As cepas utilizadas, adquiridas da coleção da FIOCRUZ, foram: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis, Serratia marscecens, Staphylococcus aureus, Enterococcus faecalis e Enterococcus faecium. No estudo, foram utilizados o caldo Mueller Hinton (MH), Extrato de Cranberry e as bactérias patogênicas. O ensaio foi realizado em triplicata, com o uso de um controle de crescimento dos micro-organismos e o experimento para avaliação do crescimento bacteriano na presença do extrato. A turbidez foi medida com o auxílio de um espectrofotômetro, no comprimento de onda de 600 nm, antes e após 24 horas de incubação à 37 ºC. O procedimento forneceu a Densidade Ótica, do qual possibilitou a identificação da inibição microbiana. Para análise estatística foi utilizado o Teste t de Student. O Extrato de Cranberry apresentou atividade antimicrobiana sobre as bactérias Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Serratia marscecens e Enterococcus faecalis (p < 0,05), confirmando seu efeito benéfico em infecções urinárias. No entanto, não teve efeito inibitório significativo sobre Pseudomonas aeruginosa, Proteus mirabilis e Enterococcus faecium (p > 0,05).

scholarly journals Actividad antibacteriana in vitro de aceites esenciales de diferentes especies del género Citrus

2017 ◽  
Vol 46 (2) ◽  
Author(s):  
Miladys Esther Torrenegra Alarcón ◽  
Nerlis Paola Pájaro ◽  
Glicerio León Méndez
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Staphylococcus Epidermidis ◽  
E Coli

Se evaluó la actividad antibacteriana in vitro de aceites esenciales de diferentes especiesdel género Citrus frente a cepas ATCC de Staphylococcus aureus, Staphylococcus epidermidis,Klebsiella pneumoniae, Pseudomonas aeruginosa y Escherichia coli, determinandola concentración mínima inhibitoria (CMI) y la concentración mínima bactericida(CMB). Las bacterias se replicaron en medios de agar y caldos específicos. Se determinóel momento de máxima densidad óptica (DO620) para emplearlo como tiempode incubación; luego se hicieron pruebas de evaluación de sensibilidad con la exposiciónde las cepas a concentraciones a 1000 g/mL del extracto en caldo. Para solubilizarse empleó dimetilsulfóxido (DMSO) al 1%. Posteriormente, se le determinó laconcentración mínima inhibitoria mediante metodologías de microdilución en caldoy la concentración mínima bactericida. Encontrándose una actividad de los aceitesesenciales del género Citrus, con valores de CMI ≥ 600 mg/mL frente a S. aureus,S. epidermidis, K. pneumoniae, P. aeruginosa y E. coli. En función a los resultados obtenidos,se concluye que las diferentes especies del género Citrus son consideradas comopromisorias para el control del componente bacteriano.

scholarly journals Antibacterial effect of Dimethyl sulfoxide extract of Aloe vera (Aloe barbadensis) leaf gel against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae

Mediscope ◽  
2020 ◽  
Vol 7 (2) ◽  
pp. 67-74 ◽  
Author(s):  
Syed Didarul Haque ◽  
Abu Md Mayeenuddin Al Amin ◽  
Baishakhi Islam ◽  
Nazia Nazneen ◽  
Syeda Noorjahan Karim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Dimethyl Sulfoxide ◽  
Antibacterial Effect ◽  
Aloe Vera ◽  
Biologically Active ◽  
Diffusion Method ◽  
E Coli

An exploratory study based on laboratory experiment was carried out to determine the antibacterial effect of Dimethyl sulfoxide (DMSO) extract of Aloe vera leaf gel (DAE) against standard strains of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae in the Department of Pharmacology & Therapeutics in collaboration with the Department of Microbiology, Mymensingh Medical College, Mymensingh, Bangladesh. DMSO extract was used in five different concentrations (100, 200, 300, 400 and 500 μg/ml). Dose dependent inhibitory effect was seen against the test organisms using disc diffusion method. Zone of inhibition (ZOI) were 8 mm, 13 mm, 15 mm, 16 mm and 21 mm against S. aureus; 0 mm, 8 mm, 13 mm, 15 mm and 18 mm against P. aeruginosa; 8 mm, 11 mm, 13 mm, 16 mm and 20 mm against E. coli; 0 mm, 9 mm, 12 mm, 14 mm and 18 mm against K. pneumoniae at 100, 200, 300, 400 and 500 μg/ml respectively. The minimum inhibitory concentration (MIC) was assessed by broth dilution technique. The MICs of DAE for S. aureus, P. aeruginosa, E. coli and K. pneumoniae were 300 μg/ml, 400 μg/ml, 400 μg/ml and 450 μg/ml respectively. From the study it was observed that DMSO extract of Aloe vera leaf gel possesses antibacterial effect against the test pathogens. The findings highlight the need for further extensive study to detect and isolate the biologically active ingredients present in the Aloe vera leaves which are responsible for antibacterial effect. Hopefully, that would lead to the discovery of new and more potent antimicrobial agents originated from Aloe vera. Mediscope Vol. 7, No. 2: July 2020, Page 67-74

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