scholarly journals Improved Properties of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Produced byComamonassp. EB172 Utilizing Volatile Fatty Acids by Regulating the Nitrogen Source

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Mohd Rafein Zakaria ◽  
Hidayah Ariffin ◽  
Suraini Abd-Aziz ◽  
Mohd Ali Hassan ◽  
Yoshihito Shirai
Keyword(s):  
Fatty Acids ◽  
Yeast Extract ◽  
Volatile Fatty Acids ◽  
Carbon Materials ◽  
Average Molecular Weight ◽  
Culture Broth ◽  
Number Average Molecular Weight ◽  
Bacterial Isolates ◽  
Carbon To Nitrogen Ratio ◽  
Nitrogen Ratio

This study presents the effect of carbon to nitrogen ratio (C/N) (mol/mol) on the cell growth and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) accumulation byComamonassp. EB172 in 2 L fermenters using volatile fatty acids (VFA) as the carbon source. This VFA was supplemented with ammonium sulphate and yeast extract in the feeding solution to achieve C/N (mol/mol) 5, 15, 25, and 34.4, respectively. By extrapolating the C/N and the source of nitrogen, the properties of the polymers can be regulated. The number average molecular weight (Mn) of P(3HB-co-3HV) copolymer reached the highest at 838 × 103 Da with polydispersity index (PDI) value of 1.8, when the culture broth was supplemented with yeast extract (C/N 34.4). Tensile strength and Young’s modulus of the copolymer containing 6–8 mol% 3HV were in the ranges of 13–14.4 MPa and 0.26–0.34 GPa, respectively, comparable to those of polyethylene (PE). Thus,Comamonassp. EB172 has shown promising bacterial isolates producing polyhydroxyalkanoates from renewable carbon materials.

Study of the methanogenic degradation of phenol via carboxylation to benzoate

1991 ◽  
Vol 37 (7) ◽  
pp. 573-576 ◽  
Author(s):  
Jean-Guy Bisaillon ◽  
François Lépine ◽  
Réjean Beaudet
Keyword(s):  
Fatty Acids ◽  
Sodium Bicarbonate ◽  
Key Words ◽  
Yeast Extract ◽  
Volatile Fatty Acids ◽  
Bacterial Consortium ◽  
Proteose Peptone ◽  
Benzoate Degradation ◽  
Dead End ◽  
Cyclohexane Carboxylate

An anaerobic bacterial consortium carboxylating phenol to benzoate by cometabolism in the presence of proteose peptone under methanogenic conditions was studied. Yeast extract or a mixture of tryptophan and lysine could replace proteose peptone without affecting the carboxylating activity, whereas glucose, glycerol, pyruvate, volatile fatty acids, and sodium bicarbonate could not. The carboxylating microorganism could not be obtained pure from the phenol culture supplemented with tryptophan and lysine; six different morphological types of microorganisms were able to grow in this medium. The results obtained with potential intermediates of benzoate degradation given as substrates to the consortium suggest that benzoate is transformed to 1-cyclohexene carboxylate and to heptanoate. Part of 1-cyclohexene carboxylate was transformed to an apparent dead-end product identified as cyclohexane carboxylate. Key words: phenol, carboxylation, benzoate, methanogenic conditions.

Unravelling a microbial synergy to boost caproate production via carboxylates chain elongation with ethanol

2019 ◽  
Vol 26 (2) ◽  
pp. 63-71
Author(s):  
Ling Leng ◽  
Ying Wang ◽  
Peixian Yang ◽  
Takashi Narihiro ◽  
Masaru Konishi Nobu ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Microbial Consortia ◽  
Chain Elongation ◽  
Desulfovibrio Vulgaris ◽  
Rrna Gene ◽  
Selective Enrichment ◽  
Microbial Network ◽  
Cost Efficient ◽  
Rrna Gene Sequencing

Chain elongation of volatile fatty acids for medium chain fatty acids production (e.g. caproate) is an attractive approach to treat wastewater anaerobically and recover resource simultaneously. Undefined microbial consortia can be tailored to achieve chain elongation process with selective enrichment from anaerobic digestion sludge, which has advantages over pure culture approach for cost-efficient application. Whilst the metabolic pathway of the dominant caproate producer, Clostridium kluyveri, has been annotated, the role of other coexisting abundant microbiomes remained unclear. To this end, an ethanol-acetate fermentation inoculated with fresh digestion sludge at optimal conditions was conducted. Also, physiological study, thermodynamics and 16 S rRNA gene sequencing to elucidate the biological process by linking the system performance and dominant microbiomes were integrated. Results revealed a possible synergistic network in which C. kluyveri and three co-dominant species, Desulfovibrio vulgaris, Fusobacterium varium and Acetoanaerobium sticklandii coexisted. D. vulgaris and A. sticklandii (F. varium) were likely to boost the carboxylates chain elongation by stimulating ethanol oxidation and butyrate production through a syntrophic partnership with hydrogen (H2) serving as an electron messenger. This study unveils a synergistic microbial network to boost caproate production in mixed culture carboxylates chain elongation.

МЕТАБОЛИТЫ ПРОБИОТИЧЕСКИХ БАКТЕРИЙ ОТВЕЧАЮТ ЗА ЭФФЕКТИВНОСТЬ ДЕЙСТВИЯ ПРОБИОТИКА "ПРОФОРТ®"

2019 ◽  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Broiler Chicks ◽  
Bacillus Sp

МЕЛИКИДИ В.Х., ТЮРИНА Д.Г., СЕЛИВАНОВ Д.Г., НОВИКОВА Н.И. ООО «БИОТРОФ», Санкт-Петербург Аннотация: Приведены данные исследования методом газожидкостной хроматомасс-спектрометрии метаболитов, синтезируемых пробиотическими бактериями, входящими в состав кормовой добавки «Профорт®». Проведен опыт в условиях интенсивного промышленного птицеводства по применению кормовой добавки «Профорт®» (50 тыс. голов бройлеров в группе). Среди метаболитов пробиотических штаммов Enterococcus sp. и Bacillus sp. обнаружены такие полезные вещества, как молочная кислота, уксусная, пропионовая и другие короткоцепочечные (летучие) жирные кислоты, активные пептиды. Результаты зоотехнического опыта показали, что при скармливании бройлерам пробиотика «Профорт®» (500 г/т) живая масса при убое в 40 дней была выше контроля на 6,9%, конверсия корма улучшилась на 3,0%, а европейский индекс продуктивности бройлеров - на 5,69%. Ключевые слова: ПРОБИОТИКИ, МЕТАБОЛИТЫ, ЛЕТУЧИЕ ЖИРНЫЕ КИСЛОТЫ, ЦЫПЛЯТА-БРОЙЛЕРЫ,ПРОДУКТИВНОСТЬ, PROBIOTICS, METABOLITES, VOLATILE FATTY ACIDS, BROILER CHICKS,PRODUCTIVITY

Studies on the Levels of Volatile Fatty Acids for Growing-Fattening Lambs

1962 ◽  
Vol 21 (1) ◽  
pp. 37-40 ◽  
Author(s):  
H. W. Essig ◽  
U. S. Garrigus ◽  
B. Connor Johnson
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids

Effect of Dietary Volatile Fatty Acids on Nitrogen Balance in Chickens.

1997 ◽  
Vol 34 (5) ◽  
pp. 349-351
Author(s):  
Hiroshi KAMISOYAMA ◽  
Zeng-Tao SUN ◽  
Mineo HASHIGUCHI ◽  
Yutaka ISSHIKI
Keyword(s):  
Fatty Acids ◽  
Nitrogen Balance ◽  
Volatile Fatty Acids

Influence of hydrothermal pretreatment conditions, typology of anaerobic digestion system, and microbial profile in the production of volatile fatty acids from olive mill solid waste

2021 ◽  
Vol 9 (2) ◽  
pp. 105055
Author(s):  
Yasmim Arantes da Fonseca ◽  
Nayara Clarisse Soares Silva ◽  
Adonai Bruneli de Camargos ◽  
Silvana de Queiroz Silva ◽  
Hector Javier Luna Wandurraga ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Anaerobic Digestion ◽  
Solid Waste ◽  
Volatile Fatty Acids ◽  
Hydrothermal Pretreatment ◽  
Microbial Profile ◽  
Olive Mill Solid Waste ◽  
Pretreatment Conditions ◽  
Olive Mill

scholarly journals Pilot Study of the Effects of Polyphenols from Chestnut Involucre on Methane Production, Volatile Fatty Acids, and Ammonia Concentration during In Vitro Rumen Fermentation

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 108
Author(s):  
Yichong Wang ◽  
Sijiong Yu ◽  
Yang Li ◽  
Shuang Zhang ◽  
Xiaolong Qi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Propionic Acid ◽  
Acid Content ◽  
Volatile Fatty Acids ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Quadratic Response ◽  
In Vitro Rumen Fermentation

Nutritional strategies can be employed to mitigate greenhouse emissions from ruminants. This article investigates the effects of polyphenols extracted from the involucres of Castanea mollissima Blume (PICB) on in vitro rumen fermentation. Three healthy Angus bulls (350 ± 50 kg), with permanent rumen fistula, were used as the donors of rumen fluids. A basic diet was supplemented with five doses of PICB (0%–0.5% dry matter (DM)), replicated thrice for each dose. Volatile fatty acids (VFAs), ammonia nitrogen concentration (NH3-N), and methane (CH4) yield were measured after 24 h of in vitro fermentation, and gas production was monitored for 96 h. The trial was carried out over three runs. The results showed that the addition of PICB significantly reduced NH3-N (p < 0.05) compared to control. The 0.1%–0.4% PICB significantly decreased acetic acid content (p < 0.05). Addition of 0.2% and 0.3% PICB significantly increased the propionic acid content (p < 0.05) and reduced the acetic acid/propionic acid ratio, CH4 content, and yield (p < 0.05). A highly significant quadratic response was shown, with increasing PICB levels for all the parameters abovementioned (p < 0.01). The increases in PICB concentration resulted in a highly significant linear and quadratic response by 96-h dynamic fermentation parameters (p < 0.01). Our results indicate that 0.2% PICB had the best effect on in-vitro rumen fermentation efficiency and reduced greenhouse gas production.

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