scholarly journals Comparison of VIDAS and Radioimmunoassay Methods for Measurement of Cortisol Concentration in Bovine Serum

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Daniela Proverbio ◽  
Roberta Perego ◽  
Eva Spada ◽  
Giada Bagnagatti de Giorgi ◽  
Angelo Belloli ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Bovine Serum ◽  
Serum Cortisol ◽  
Cortisol Concentration ◽  
Altman Plot ◽  
Gold Standard Method ◽  
Bland Altman Plot ◽  
Deming Regression ◽  
Significant Difference ◽  
Serum Cortisol Concentration

Radioimmunoassay (RIA) is the “gold standard” method for evaluation of serum cortisol concentration. The VIDAS cortisol test is an enzyme-linked fluorescent assay designed for the MiniVidas system. The aim of this study was to compare the VIDAS method with RIA for measurement of bovine serum cortisol concentration. Cortisol concentrations were evaluated in 40 cows using both VIDAS and RIA methods, the latter as the reference method. A paired Student’s -test, Pearson’s correlation analysis, Bland-Altman plot, and Deming regression analysis were used to compare the two methods. There was no statistically significant difference between mean serum cortisol concentrations measured by VIDAS or RIA methods (). Both methods were able to detect significant differences in mean low and high cortisol concentrations ( RIA and VIDAS). The correlation coefficient was low, but a Bland-Altman plot and Deming regression analysis show neither constant nor proportional error. The VIDAS method produced slightly higher values than RIA, but the difference was small and in no case did the mean value move the normal range. Results suggest that VIDAS method is suitable for the determination of bovine serum cortisol concentration in studies of large numbers of animals.

scholarly journals Comparison of the Vcheck and IMMULITE 2000 methods for cortisol measurement in canine serum

2021 ◽  
Vol 77 (08) ◽  
pp. 6562-2021
Author(s):  
ŁUKASZ ADASZEK ◽  
OLIWIER TEODOROWSKI ◽  
MARTA STANIEC ◽  
RADOSŁAW JANECKI ◽  
NATALIA JACKOWSKA-PEJKO
Keyword(s):  
Regression Analysis ◽  
Reference Method ◽  
Serum Cortisol ◽  
Cortisol Concentration ◽  
Serum Samples ◽  
Altman Plot ◽  
Bland Altman Plot ◽  
Simple Regression Analysis ◽  
Canine Serum ◽  
Adrenal Disease

The aim of this study was to compare canine cortisol results obtained by the Vcheck method with those obtained by the IMMULITE 2000 immunoassay, which had previously been validated for canine serum. The concentration of cortisol in 44 canine serum samples was determined concurrently by the Vcheck and IMMULITE methods, the latter as the reference method. Cortisol values were compared using Pearson’s correlation analysis and simple regression analysis. Agreement between the two methods was calculated with a Bland-Altman plot. According to regression analysis and the Bland-Altman plot, the two methods gave comparable results. The results of the Vcheck method were comparable to those of the IMMULITE 2000 reference method, so the Vcheck may be used as an alternative assay to evaluate serum cortisol concentration in dogs for the diagnosis of adrenal disease.

scholarly journals Serum cortisol concentration in cattle of different genetic groups slaughtered in confinement

2016 ◽  
Vol 37 (4Supl1) ◽  
pp. 2601 ◽  
Author(s):  
Aracele Pinheiro Pales ◽  
João Teodoro Padua ◽  
Klayto José Gonçalves Santos ◽  
Karyne Oliveira Coelho ◽  
Cláudia Peixoto Bueno ◽  
...  
Keyword(s):  
Serum Cortisol ◽  
Cortisol Concentration ◽  
Meat Tenderness ◽  
Blood Samples ◽  
The Third ◽  
Genetic Groups ◽  
Significant Difference ◽  
Shearing Strength ◽  
Low Cortisol ◽  
Serum Cortisol Concentration

The objective of this work was to compare the serum cortisol levels among four genetic cattle groups slaughtered in confinement. Thirty-two non-castrated male bovines from four genetic groups were analyzed: Nelore, Aberdeen Angus × Nelore, Caracu × Nelore, and Guzerá × Nelore. The blood samples were collected on the day of weighing (every 28 days) and during the slaughter. Regarding the concentration of serum cortisol, no significant differences were found among the groups during the first, second, and fourth collections. However, during the third collection and at the day of slaughter, respectively, the Nelore group (3.15 ng/dL; 2.98 ng/dL) showed similarity to the Caracu × Nelore (2.02 ng/dL; 2.07ng/dL) and Guzerá × Nelore (2.33 ng/dL; 2.40 ng/dL) groups. Nelore showed a significant difference compared with the Aberdeen Angus × Nelore group (1.21 ng/dL; 1.61 ng/dL). With respect to the overall collection averages, the Nelore group (2.85 ± 0.87 ng/dL) showed greater values than the Aberdeen Angus × Nelore group (1.61 ± 0.87 ng/dL). However, it showed similarities to the Caracu × Nelore (2.06 ± 1.31 ng/dL) and Guzerá × Nelore (2.48 ± 1.16 ng/dL) groups. It was also observed that animals with low cortisol values gained more weight, as demonstrated by the correlation between ADG and the cortisol collection averages (-0.54; p < 0.05). There was no difference among the groups with respect to shearing strength of the meat, and the correlation between this force and the cortisol collection average (0.15; p > 0.05, ns) showed that meat tenderness in the studied groups was not affected.

Platelet Function in Surgical Stress

1985 ◽  
Vol 54 (04) ◽  
pp. 849-852 ◽  
Author(s):  
O Naesh ◽  
J T Friis ◽  
I Hindberg ◽  
K Winther
Keyword(s):  
Stress Response ◽  
Negative Correlation ◽  
Surgical Stress ◽  
Plasma Concentrations ◽  
Serum Cortisol ◽  
Cortisol Concentration ◽  
Pulmonary Dysfunction ◽  
Arterial Po2 ◽  
Elective Cholecystectomy ◽  
Serum Cortisol Concentration

SummaryTen patients for elective cholecystectomy were studied pre-, per- and postoperatively. All had neurolept anesthesia. Plasma concentrations of β-TG, TXB2 and 5-HT and intraplatelet 5-HT were measured. Aggregation to ADP was recorded.Serum cortisol concentration was used as index of the stress response, showing peroperative increase and postoperative decrease. Closely related to this we observed a significant increase in P-β-TG and P-TXB2 with postoperative normalization in 6 patients without complications. P-5-HT had a peak peropera-tively and remained elevated postoperatively. A negative correlation between P--5-HT and decreasing intraplatelet 5-HT postoperatively was observed.High postoperative levels of P--5-HT seem to be related to low arterial Po2 and pulmonary dysfunction. In 3 patients with complications a second increase in P-β-TG, P-TXB2 and partly in P--5-HT was found. Platelets were temporarily refractory to ADP immediately following surgery and showed increased aggregabil-ity postoperatively. We conclude that platelets are activated in surgical stress.

Serum cortisol concentration in patients with major depression after treatment with clomipramine

2009 ◽  
Vol 61 (4) ◽  
pp. 604-611 ◽  
Author(s):  
Jadwiga Piwowarska ◽  
Małgorzata Wrzosek ◽  
Maria Radziwoń-Zaleska ◽  
Beata Ryszewska-Pokraśniewicz ◽  
Michał Skalski ◽  
...  
Keyword(s):  
Major Depression ◽  
Serum Cortisol ◽  
Cortisol Concentration ◽  
Serum Cortisol Concentration

scholarly journals Performance and serum cortisol concentration in Santa Inês lambs under different suckling schemes

2016 ◽  
Vol 45 (9) ◽  
pp. 546-553
Author(s):  
Ana Carolina Alves ◽  
Nadja Gomes Alves ◽  
Ivan Júnior Ascari ◽  
Felipe Barbosa Junqueira ◽  
Luciana França Smith Maciel ◽  
...  
Keyword(s):  
Serum Cortisol ◽  
Cortisol Concentration ◽  
Santa Inês ◽  
Serum Cortisol Concentration

Reproducibility of the cortisol response to stimulation with a low dose of ACTH(1–24): the effect of basal cortisol levels and comparison of low-dose with high-dose secretory dynamics

1993 ◽  
Vol 136 (1) ◽  
pp. 167-172 ◽  
Author(s):  
S. Crowley ◽  
P. C. Hindmarsh ◽  
J. W. Honour ◽  
C. G. D. Brook
Keyword(s):  
Cortisol Level ◽  
Low Dose ◽  
Standard Dose ◽  
Serum Cortisol ◽  
Cortisol Concentration ◽  
Acth Stimulation ◽  
Basal Cortisol ◽  
Basal Cortisol Level ◽  
Adrenal Response ◽  
Serum Cortisol Concentration

ABSTRACT We compared the reproducibility and repeatability of the acute adrenal response to low doses (90 and 500 ng/1·73 m2) of Synacthen (ACTH(1–24)) with that of the standard dose (250 μg/1·73 m2). We also examined the effect of basal cortisol levels on peak values achieved after stimulation with a low dose. ACTH(1–24) was given to six male volunteers: 90 ng/1·73 m2 twice at 90-min intervals on day 1, and 90 and 500 ng/1·73 m2 once on day 2 and 250 μg/1·73 m2 once on day 3. The rise in serum cortisol concentration with repeated low doses of ACTH was not attenuated (161 ± 49 (s.d.) nmol/l on initial vs 150 ± 41 nmol/l on repeat stimulation; P = 0·5) and this was reproducible (161 ± 49 nmol/l on day 1 vs 148 ± 15 nmol/l on day 2; P = 0·6). A dose of 500 ng ACTH(1–24)/1·73 m2 produced a maximal adrenal response in that the rise in serum cortisol concentration at 20 min was identical with that produced at the same time by the standard dose of 250 μg/1·73 m2. There was a strong positive correlation between the basal cortisol level and peak cortisol concentration after low-dose ACTH stimulation (r = 0·93, P < 0·001) but not between the basal cortisol level and the incremental rise (r= −0·1, P = 0·69). These results suggest that the cortisol response to low-dose ACTH stimulation is reproducible and not attenuated by repeat stimulation at 90-min intervals. The incremental rise in serum cortisol concentration after ACTH stimulation appears constant in these situations and is not influenced by the basal cortisol level. When there is concern that the standard dose may be excessive and mask subtle but important changes in adrenal function, the low dose (500 ng) of ACTH should be used. Journal of Endocrinology (1993) 136, 167–172

Serum cortisol concentration in patients with major depression after treatment with fluoxetine

2012 ◽  
Vol 198 (3) ◽  
pp. 407-411 ◽  
Author(s):  
Jadwiga Piwowarska ◽  
Aneta Chimiak ◽  
Halina Matsumoto ◽  
Anna Dziklińska ◽  
Maria Radziwoń-Zaleska ◽  
...  
Keyword(s):  
Major Depression ◽  
Serum Cortisol ◽  
Cortisol Concentration ◽  
Serum Cortisol Concentration
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