scholarly journals Bioactive Volatile Content of the Stem and Root ofCentaurea carduiformisDC. subsp.carduiformisvar.carduiformis

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Ibrahim Demirtas ◽  
Ayse Sahin
Keyword(s):  
Cell Lines ◽  
Vero Cells ◽  
Hexane Extract ◽  
African Green Monkey Kidney ◽  
Brain Tumor Cells ◽  
Green Monkey ◽  
Rat Brain Tumor ◽  
Medicinal Properties ◽  
Asteraceae Family

Several species ofCentaurea, biennial (or more rarely perennial) plants which belong to the Asteraceae family, possess medicinal properties and are currently used in phytotherapy. In the present study, antiproliferative activity of hexane extract from stems and roots ofCentaurea carduiformisDC. subsp.carduiformisvar.carduiformis(CCS and CCR) on Vero cells (African green monkey kidney), C6 cells (Rat Brain tumor cells), and HeLa cells (human uterus carcinoma) was investigatedin vitro. Antiproliferative effect of the extract was tested at 500 μg/mL and 1000 μg/mL using BrDu Cell Proliferation ELISA. The hexane extract was significantly inhibited proliferation of Vero, HeLa, and C6 cancer cell lines with absorbance values. The extract of CCS and CCR showed the highest activity against the Vero, HeLa, and C6 cell lines at 500 μg/mL and 1000 μg/mL.

Chemical constituents and antiproliferative effects of cultured Mougeotia nummuloides and Spirulina major against cancerous cell lines

2016 ◽  
Vol 71 (3-4) ◽  
pp. 87-92 ◽  
Author(s):  
Ramazan Erenler ◽  
Koksal Pabuccu ◽  
Ayse Sahin Yaglioglu ◽  
Ibrahim Demirtas ◽  
Fatih Gul
Keyword(s):  
Cell Lines ◽  
Chemical Constituents ◽  
Vero Cells ◽  
C6 Cells ◽  
African Green Monkey Kidney ◽  
Cancerous Cell ◽  
Brain Tumor Cells ◽  
Rat Brain Tumor ◽  
High Concentrations

AbstractIn this study, the effect ofMougeotia nummuloidesandSpirulina majoron Vero cells (African green monkey kidney), C6 cells (rat brain tumor cells) and HeLa cells (human uterus carcinoma) was investigatedin vitro. The antiproliferative effect of the methanol extract ofM. nummuloidesandS. majorcompared with 5-fluorourasil (5-FU) and cisplatin was tested at various concentrations using the BrdU Cell Proliferation ELISA. BothM. nummuloidesandS. majorextracts significantly inhibited the proliferation of Vero, HeLa and C6 cancer cell lines with IC50and IC75values. TheM. nummuloidesextract exhibited higher activity than 5-FU and cisplatin on Vero and C6 cells at high concentrations. TheS. majorextract revealed better antifproliferative activity than standards against Vero cells at 500 μg/mL. The compounds of methanol extracts were determined by GC-MS after the silylation process. Trehalose, monostearin and 1-monopalmitin were detected as major products in theM. nummuloidesextract where as in theS. majorextract; monostearin, 1-monopalmitin and hexyl alcohol were the main constituents.

scholarly journals The isolation of bioactive flavonoids from Jacaranda obtusifolia H. B. K. ssp. rhombifolia (G. F. W. Meijer) Gentry

2012 ◽  
Vol 62 (2) ◽  
pp. 181-190 ◽  
Author(s):  
Sorachai Khamsan ◽  
Saisunee Liawruangrath ◽  
Aphiwat Teerawutkulrag ◽  
Stephen Pyne ◽  
Mary Garson ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Structure Elucidation ◽  
Vero Cells ◽  
2D Nmr ◽  
Small Cell ◽  
Small Cell Lung ◽  
African Green Monkey Kidney ◽  
Green Monkey ◽  
C Nmr

The isolation of bioactive flavonoids from Jacaranda obtusifolia H. B. K. ssp. rhombifolia (G. F. W. Meijer) Gentry The paper describes the bioassay-guided isolation, structure elucidation and anticancer evaluation of five flavonoids (-)-liquiritigenin (1), (-)-neoliquiritin (2), isoliquiritigenin (3), isoliquiritin (4) and formononetin (5) from the twigs of Jacaranda obtusifolia H. B. K. ssp. rhombifolia (G. F. W. Meijer) Gentry. The structures were elucidated based on 1H, 13C NMR, comprehensive 2D NMR, MS analyses and comparison with previously reported spectral data. Compounds 1 and 3 were demonstrated to be inhibitory in vitro against NCI-H187 (small cell lung cancer) with IC50 values of 30.1 and 16.6 μg mL-1, respectively. The isolates were non-cytotoxic to Vero cells (African green monkey kidney).

scholarly journals Low-pH Endocytic Entry of the Porcine Alphaherpesvirus Pseudorabies Virus

2018 ◽  
Vol 93 (2) ◽  
Author(s):  
Jonathan L. Miller ◽  
Darin J. Weed ◽  
Becky H. Lee ◽  
Suzanne M. Pritchard ◽  
Anthony V. Nicola
Keyword(s):  
Cell Lines ◽  
Pseudorabies Virus ◽  
Vero Cells ◽  
Low Ph ◽  
Host Cells ◽  
Multiple Model ◽  
Trade Restrictions ◽  
African Green Monkey Kidney ◽  
Entry Pathway ◽  
Model Cell

ABSTRACTThe alphaherpesvirus pseudorabies virus (PRV) is the causative agent of pseudorabies, a disease of great economic and welfare importance in swine. Other alphaherpesviruses, including herpes simplex virus (HSV), utilize low-pH-mediated endocytosis to enter a subset of cell types. We investigated whether PRV used this entry pathway in multiple laboratory model cell lines. Inhibition of receptor-mediated endocytosis by treatment with hypertonic medium prevented PRV entry. PRV entry into several cell lines, including porcine kidney (PK15) cells and African green monkey kidney (Vero) cells, was inhibited by noncytotoxic concentrations of the lysosomotropic agents ammonium chloride and monensin, which block the acidification of endosomes. Inactivation of virions by acid pretreatment is a hallmark of viruses that utilize a low-pH-mediated entry pathway. Exposure of PRV virions to pH 5.0 in the absence of host cell membranes reduced entry into PK15 and Vero cells by >80%. Together, these findings suggest that endocytosis followed by fusion with host membranes triggered by low endosomal pH is an important route of entry for PRV.IMPORTANCEPRV is a pathogen of great economic and animal welfare importance in many parts of the world. PRV causes neurological, respiratory, and reproductive disorders, often resulting in mortality of young and immunocompromised animals. Mortality, decreased production, and trade restrictions result in significant financial losses for the agricultural industry. Understanding the molecular mechanisms utilized by PRV to enter host cells is an important step in identifying novel strategies to prevent infection and spread. A thorough understanding of these mechanisms will contribute to a broader understanding of alphaherpesvirus entry. Here, we demonstrate PRV entry into multiple model cell lines via a low-pH endocytosis pathway. Together, these results provide a framework for elucidating the early events of the PRV replicative cycle.

scholarly journals Attachment and invasion ofToxoplasma gondiiandNeospora caninumto epithelial and fibroblast cell linesin vitro

Parasitology ◽  
2005 ◽  
Vol 131 (5) ◽  
pp. 583-590 ◽  
Author(s):  
YING LEI ◽  
M. DAVEY ◽  
J. T. ELLIS
Keyword(s):  
Cell Lines ◽  
Neospora Caninum ◽  
Fibroblast Cell ◽  
Cell Types ◽  
Vero Cells ◽  
Host Cells ◽  
Trypsin Treatment ◽  
Epithelial Cell Lines ◽  
Pre Treatment

Attachment and invasion ofToxoplasma gondiiandNeospora caninumto a cat and a dog fibroblast cell line and 2 epithelial cell lines (a cat kidney and Vero) were comparedin vitrousing fluorescence antibody methodology. In addition, trypsin treatment of tachyzoites was used to determine whether protein molecules were essential to the process of invasion. The results show that bothT. gondiiandN. caninuminvaded all 4 cell lines, and that pre-treatment ofT. gondiitachyzoites with trypsin caused an increase in the ability of the parasite to invade these host cells. FurthermoreT. gondii, in comparison toN. caninum, invaded all 4 cell lines at greater levels. The results here support the conclusion that bothT. gondiiandN. caninumhave the ability to invade a variety of cell types including both dog and cat cells, and questions the utility of Vero cells as an appropriate host cell forin vitrostudies on the biology of these taxa.

scholarly journals Cytotoxic and Genotoxic Effects of Fluconazole on African Green Monkey Kidney (Vero) Cell Line

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Regianne Maciel dos Santos Correa ◽  
Tatiane Cristina Mota ◽  
Adriana Costa Guimarães ◽  
Laís Teixeira Bonfim ◽  
Rommel Rodriguez Burbano ◽  
...  
Keyword(s):  
Comet Assay ◽  
Vero Cell ◽  
Cell Line ◽  
Vero Cells ◽  
Negative Control ◽  
African Green Monkey ◽  
Monkey Kidney ◽  
African Green Monkey Kidney ◽  
Vero Cell Line ◽  
Green Monkey

Fluconazole is a broad-spectrum triazole antifungal that is well-established as the first-line treatment for Candida albicans infections. Despite its extensive use, reports on its genotoxic/mutagenic effects are controversial; therefore, further studies are needed to better clarify such effects. African green monkey kidney (Vero) cells were exposed in vitro to different concentrations of fluconazole and were then evaluated for different parameters, such as cytotoxicity (MTT/cell death by fluorescent dyes), genotoxicity/mutagenicity (comet assay/micronucleus test), and induction of oxidative stress (DCFH-DA assay). Fluconazole was used at concentrations of 81.6, 163.2, 326.5, 653, 1306, and 2612.1μM for the MTT assay and 81.6, 326.5, and 1306μM for the remaining assays. MTT results showed that cell viability reduced upon exposure to fluconazole concentration of 1306μM (85.93%), being statistically significant (P<0.05) at fluconazole concentration of 2612.1μM (35.25%), as compared with the control (100%). Fluconazole also induced necrosis (P<0.05) in Vero cell line when cells were exposed to all concentrations (81.6, 326.5, and 1306μM) for both tested harvest times (24 and 48 h) as compared with the negative control. Regarding genotoxicity/mutagenicity, results showed fluconazole to increase significantly (P<0.05) DNA damage index, as assessed by comet assay, at 1306μM versus the negative control (DI=1.17 vs DI=0.28, respectively). Micronucleus frequency also increased until reaching statistical significance (P<0.05) at 1306μM fluconazole (with 42MN/1000 binucleated cells) as compared to the negative control (13MN/1000 binucleated cells). Finally, significant formation of reactive oxygen species (P<0.05) was observed at 1306μM fluconazole vs the negative control (OD=40.9 vs OD=32.3, respectively). Our experiments showed that fluconazole is cytotoxic and genotoxic in the assessed conditions. It is likely that such effects may be due to the oxidative properties of fluconazole and/or the presence of FMO (flavin-containing monooxygenase) in Vero cells.

Use of verapamil to enhance the antiproliferative activity of BCNU in human glioma cells: an in vitro and in vivo study

1990 ◽  
Vol 73 (2) ◽  
pp. 248-253 ◽  
Author(s):  
Alfred P. Bowles ◽  
Cooley G. Pantazis ◽  
William Wansley
Keyword(s):  
Tumor Growth ◽  
Cell Lines ◽  
Antiproliferative Activity ◽  
Human Glioma ◽  
Content Type ◽  
Brain Tumor Cells ◽  
Inhibition Of Tumor Growth ◽  
Dose Dependent

✓ The authors have evaluated the antiproliferative activity of verapamil, alone or in combination with 1, 3-bis(2-chloroethyl)-1-nitrosourea (BCNU) in brain-tumor cells. These effects were studied in vitro using four human glioma cell lines and in vivo using glioblastoma multiforme cells transplanted to athymic nude mice. The results showed that verapamil when used alone produced inhibition of tumor growth; however, when verapamil was used in combination with BCNU (in vitro), significant dose-dependent suppression of proliferation occurred in all four cell lines. The in vivo results were far more dramatic. Mice treated with BCNU (25 mg/kg) plus verapamil (50 mg/kg) achieved a 200-fold decrease in tumor growth with a greater than 80% regression in tumor size. Complete cures were achieved in 80% of the mice observed for at least 50 days following the completion of therapy. These findings support the use of verapamil in overcoming drug resistance in malignant brain tumors.

scholarly journals Concurrent Replication and Methylation at Mammalian Origins of Replication

1998 ◽  
Vol 18 (6) ◽  
pp. 3475-3482 ◽  
Author(s):  
Felipe D. Araujo ◽  
J. David Knox ◽  
Moshe Szyf ◽  
Gerald B. Price ◽  
Maria Zannis-Hadjopoulos
Keyword(s):  
Cell Lines ◽  
Mammalian Cells ◽  
Cytosine Methylation ◽  
Nearest Neighbor ◽  
African Green Monkey Kidney ◽  
Origins Of Replication ◽  
Initiation Of Replication ◽  
Green Monkey ◽  
Primary Cell Lines ◽  
Genomic Dnas

ABSTRACT Observations made with Escherichia coli have suggested that a lag between replication and methylation regulates initiation of replication. To address the question of whether a similar mechanism operates in mammalian cells, we have determined the temporal relationship between initiation of replication and methylation in mammalian cells both at a comprehensive level and at specific sites. First, newly synthesized DNA containing origins of replication was isolated from primate-transformed and primary cell lines (HeLa cells, primary human fibroblasts, African green monkey kidney fibroblasts [CV-1], and primary African green monkey kidney cells) by the nascent-strand extrusion method followed by sucrose gradient sedimentation. By a modified nearest-neighbor analysis, the levels of cytosine methylation residing in all four possible dinucleotide sequences of both nascent and genomic DNAs were determined. The levels of cytosine methylation observed in the nascent and genomic DNAs were equivalent, suggesting that DNA replication and methylation are concomitant events. Okazaki fragments were also demonstrated to be methylated, suggesting that the rapid kinetics of methylation is a feature of both the leading and the lagging strands of nascent DNA. However, in contrast to previous observations, neither nascent nor genomic DNA contained detectable levels of methylated cytosines at dinucleotide contexts other than CpG (i.e., CpA, CpC, and CpT are not methylated). The nearest-neighbor analysis also shows that cancer cell lines are hypermethylated in both nascent and genomic DNAs relative to the primary cell lines. The extent of methylation in nascent and genomic DNAs at specific sites was determined as well by bisulfite mapping of CpG sites at the lamin B2, c-myc, and β-globin origins of replication. The methylation patterns of genomic and nascent clones are the same, confirming the hypothesis that methylation occurs concurrently with replication. Interestingly, the c-mycorigin was found to be unmethylated in all clones tested. These results show that, like genes, different origins of replication exhibit different patterns of methylation. In summary, our results demonstrate tight coordination of DNA methylation and replication, which is consistent with recent observations showing that DNA methyltransferase is associated with proliferating cell nuclear antigen in the replication fork.

Sign in / Sign up

Export Citation Format

Share Document