scholarly journals Analysis of protein from nasopharyngeal carcinoma cell line CNE2 irradiated by X-ray using Raman spectroscopy

2012 ◽  
Vol 27 (1) ◽  
pp. 1-7
Author(s):  
Lin Ou ◽  
Yang Chen ◽  
Yangwen Huang ◽  
Ying Su ◽  
Duo Lin ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Nasopharyngeal Carcinoma ◽  
Cell Line ◽  
Total Protein ◽  
Potential Method ◽  
Support Vector ◽  
X Ray ◽  
Paired Samples ◽  
Rs Analysis ◽  
Different Doses

Our work aims to explore Raman spectroscopy (RS) to study the effects of total protein of nasopharyngeal carcinoma (NPC) cell line CNE2 treated by different doses of X-ray irradiation. The total protein is extracted from the cell line after irradiation with no incubation (0 h) and with incubation for 72 h, respectively. Both paired-samplesttest and support vector machine (SVM) are employed for statistical analysis of Raman spectrum. The results show that several Raman bands assigned to total protein can be detected; and the X-ray irradiation have various effects on different components in total protein, especially for the dose higher than 6 Gy, and also associated with the incubation time after irradiation. The differences of content between 0 h groups and 72 h groups appear at bands of C–S stretching, C–H stretching, O=C–O−stretching and L-phenylalanine. It indicated no significant diversification on structures of protein, while the content may be varied during irradiation. Our spectroscopic results reveal that RS analysis of total protein of cell line irradiated by X-ray, in conjunction with mathematical statistical model, can be a potential method to explore the Raman characterization target of cell radiosensitivity, and then for making known the mechanism of radiosensitivity of tumor further.

Raman spectral analysis of nasopharyngeal carcinoma cell line CNE2 after microwave radiation

2013 ◽  
Vol 91 (2) ◽  
pp. 67-71 ◽  
Author(s):  
Yuhuang Ye ◽  
Yang Chen ◽  
Ying Su ◽  
Changyan Zou ◽  
Yangwen Huang ◽  
...  
Keyword(s):  
Support Vector Machine ◽  
Raman Spectroscopy ◽  
Nasopharyngeal Carcinoma ◽  
Microwave Radiation ◽  
Principal Components ◽  
Carcinoma Cell ◽  
Support Vector ◽  
Components Analysis ◽  
Raman Spectral ◽  
Radiation Time

This study aimed to study the effects of microwave radiation on the nasopharyngeal carcinoma cell line CNE2 by Raman spectroscopy. The cells were separated into a control group and radiated groups with radiation times of 2, 5, 10, and 25 min, respectively. Both principal components analysis and support vector machine were employed for statistical analysis of Raman spectra. The results show that the relative content of C-H deformation and amide I begin to change when the radiation time is over 10 min, and principal components analysis further confirms there are significant differences after 10 min of radiation. Moreover, support vector machine is simultaneously used to classify radiated samples from control samples. The classification accuracy is low until the radiation time reaches over 10 min. In conclusion, this study reveals the Raman spectral characteristics of CNE2 under different microwave radiation exposure timesand demonstrates Raman spectroscopy can be a potential method to explore cellular characterization after radiation. The final results may help in elucidating the mechanism by which microwave radiation interacts with tumor cells.

Label-Free Classification of a Nasopharyngeal Carcinoma Tissue Test at Different Stages Based on Raman Spectroscopy

2017 ◽  
Vol 100 (2) ◽  
pp. 429-433 ◽  
Author(s):  
Mingyu Liu ◽  
Jinyong Lin ◽  
Sufang Qiu ◽  
Weilin Wu ◽  
Gaoqiang Liu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Nasopharyngeal Carcinoma ◽  
Raman Spectra ◽  
Early Stage ◽  
Support Vector ◽  
Chemical Compositions ◽  
Label Free ◽  
Quick Method ◽  
Clinical Method

Abstract Raman spectroscopy (RS) of nasopharyngeal carcinoma (NPC) tissue provides substantial biomolecular information and various biomedicine features for tissue at different stages of cancer development. This study suggested an automatic and quick method for the classification of Raman spectra at different stages of NPC by multivariate statistical analysis. During RS measurement, Raman spectra were acquired from all NPC tissues in two groups of samples: 30 early-stage NPC patients (stages I and II) and 46 advanced-stage NPC patients (stages III and IV). In addition, a tentative diagnostic algorithm comprising principal components analysis and support vector machine was used to effectively classify multivariate data from the Raman spectra to yield sensitivities (70%; 21 of 30 samples) and specificities (91%; 42 of 46 samples) by the leave-one-out cross-validation method. Meaningful chemical compositions in the classification process were then deduced by analyzing the classified mathematical model. This beneficial work provides a great potential clinical method for the automatic classification of NPC stages and the speculation of the chemical compositions for NPC staging.

An Established Epithelial Cell Line (NPC/HK1) from a Nasopharyngeal Carcinoma - II. A Sem Study

1982 ◽  
Vol 40 ◽  
pp. 224-225
Author(s):  
Li C.L. ◽  
Chew E.C. ◽  
Huang D.P. ◽  
Ho H.C. ◽  
Mak L.S. ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epithelial Cell ◽  
Surface Morphology ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Present Communication ◽  
Cells In Culture ◽  
Sem Study ◽  
Well Differentiated

An epithelial cell line, NPC/HK1, has recently been successfully established from a nasopharyngeal carcinoma of the moderately to well differentiated squamous type. The present communication reports on the surface morphology of the NPC/HK1 cells in culture.

Raman spectroscopy and X-ray diffraction of lithium niobate at temperatures up to 1300○-4.80pt○C

2005 ◽  
Vol 126 ◽  
pp. 101-105 ◽  
Author(s):  
B. Moulin ◽  
L. Hennet ◽  
D. Thiaudière ◽  
P. Melin ◽  
P. Simon
Keyword(s):  
Raman Spectroscopy ◽  
Lithium Niobate ◽  
X Ray Diffraction ◽  
X Ray

scholarly journals Use of WetSEM® capsules for convenient multimodal scanning electron microscopy, energy dispersive X-ray analysis, and micro Raman spectroscopy characterisation of technetium oxides

2021 ◽  
Author(s):  
D. J. Bailey ◽  
M. C. Stennett ◽  
J. Heo ◽  
N. C. Hyatt
Keyword(s):  
Raman Spectroscopy ◽  
Low Cost ◽  
Powder Materials ◽  
Spectroscopy Analysis ◽  
X Ray ◽  
Micro Raman Spectroscopy ◽  
Hazard And Risk ◽  
Sem Imaging ◽  
General User ◽  
532 Nm

AbstractSEM–EDX and Raman spectroscopy analysis of radioactive compounds is often restricted to dedicated instrumentation, within radiological working areas, to manage the hazard and risk of contamination. Here, we demonstrate application of WetSEM® capsules for containment of technetium powder materials, enabling routine multimodal characterisation with general user instrumentation, outside of a controlled radiological working area. The electron transparent membrane of WetSEM® capsules enables SEM imaging of submicron non-conducting technetium powders and acquisition of Tc Lα X-ray emission, using a low cost desktop SEM–EDX system, as well as acquisition of good quality μ-Raman spectra using a 532 nm laser.

The comparison of Zn(II) arginine dithiocarbamate cytotoxicity in T47D breast cancer and fibroblast cells

2021 ◽  
pp. 1-7
Author(s):  
Prihantono Prihantono ◽  
Rizal Irfandi ◽  
Indah Raya
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Fibroblast Cell ◽  
Fibroblast Cells ◽  
X Ray ◽  
Dithiocarbamate Complex ◽  
T47d Breast Cancer Cell ◽  
Fibroblast Cell Lines

BACKGROUND: With essential metals being studied and developed as anticancer agents, this study aims to explore the anticancer activity of Zn(II) arginine dithiocarbamate in the T47D and fibroblast cell lines. METHOD: The Zn(II) arginine dithiocarbamate complex was prepared by the in situ method and characterized using infra-red spectroscopy, melting point, X-ray fluorescence, and X-ray diffraction instruments. The complex compound was tested for its cytotoxicity to the T47D breast cancer and fibroblast cell lines. RESULTS: The cytotoxicity of the Zn(II) arginine dithiocarbamate complex to the T47D breast cancer cell line obtained IC50 = 3.16 μg/mL, while cisplatin obtained IC50 = 28.18 μg/mL. The cytotoxicity of the Zn(II) arginine dithiocarbamate complex to fibroblast cells obtained IC50 = 8709.63 μg/mL. CONCLUSION: The Zn(II) arginine dithiocarbamate complex has increased active cytotoxicity compared to cisplatin in inducing morphological changes in the T47D breast cancer cell line and is relatively non-toxic to fibroblast cells.

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