scholarly journals Genetic Predisposition for Dermal Problems in Hexavalent Chromium Exposed Population

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Priti Sharma ◽  
Vipin Bihari ◽  
Sudhir K. Agarwal ◽  
Sudhir K. Goel
Keyword(s):  
Health Status ◽  
Hexavalent Chromium ◽  
Genetic Predisposition ◽  
Dna Isolation ◽  
Confounding Effect ◽  
Blood Samples ◽  
Symptomatic Group ◽  
Exposed Population ◽  
Reference Community

We studied the effect of genetic susceptibility on hexavalent chromium induced dermal adversities. The health status of population was examined from the areas of Kanpur (India) having the elevated hexavalent chromium levels in groundwater. Blood samples were collected for DNA isolation to conduct polymorphic determination of genes, namely:NQO1(C609T),hOGG1(C1245G),GSTT1,andGSTM1(deletion). Symptomatic exposed subjects(n=38)were compared with asymptomatic exposed subjects(n=108)along with asymptomatic controls(n=148)from a non contaminated reference community. Exposed symptomatic group consisted of 36.8% subjects who wereGSTM1null genotyped as compared to asymptomatic where only 19.4% subjects were null. The exposed subjects withGSTM1null genotype were more susceptible to dermal adversities in comparison with wild genotyped subjects (OR = 2.42; 95% CI = 1.071–5.451). Age, smoking, gender or duration of residence were not found to have any confounding effect towards this association. Association with other genes was not statistically significant, nonetheless, possible contribution by these genes cannot be ruled out. In conclusion, variation in the polymorphic status ofGSTM1gene may influence dermal outcomes among residents from Cr(VI) contaminated areas. Further studies are therefore, needed to examine these observations among different population groups.

Synthesis and solvatochromic studies of 2- amino-3-phenolazo1-(4-sulfophenyl)-3-methyi-5-pyrozolone and use it for the determination of trace amount of Nickel (II) in blood samples

2016 ◽  
Vol 5 (10) ◽  
pp. 4920
Author(s):  
Amar M. Ali ◽  
Hussain. J. Mohammed*
Keyword(s):  
Stability Constant ◽  
Trace Amount ◽  
Benzenesulfonic Acid ◽  
Determination Method ◽  
Blood Samples ◽  
Determination Of Nickel ◽  
Normal Human ◽  
The Stability ◽  
Normal Human Blood

A new, simple, sensitive and rapid spectrophotometric method is proposed for the determination of trace amount of Nickel (II). The method is based on the formation of a 1:2 complex with 4-(4-((2-hydroxy-6-nitrophenyl) diazenyl) -3-methyl-5-oxo-2, 5-dihydro-1H-pyrazol-1-yl) benzenesulfonic acid (2-ANASP) as a new reagent is developed. The complex has a maximum absorption at 516 nm and εmax of 1. 84 X 105 L. mol-1. cm-1. A linear correlation (0. 25 – 4. 0μg. ml-1) was found between absorbance at λmax and concentration. The accuracy and reproducibility of the determination method for various known amounts of Nickel (II) were tested. The results obtained are both precise (RSD was 1. 2 %) and accurate (relative error was 0. 787 %). The effect of diverse ions on the determination of Nickel (II) to investigate the selectivity of the method were also studied. The stability constant of the product was 0. 399 X 106 L. mol-1. The proposed method was successfully applied to the analysis of diabetes blood and normal human blood. 

scholarly journals Determination of 19 Psychoactive Substances in Premortem and Postmortem Whole Blood Samples Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry

Separations ◽  
2021 ◽  
Vol 8 (6) ◽  
pp. 78
Author(s):  
Sevasti Karampela ◽  
Jessica Smith ◽  
Irene Panderi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Formic Acid ◽  
Whole Blood ◽  
High Performance ◽  
Tandem Mass ◽  
Psychoactive Substances ◽  
Blood Samples ◽  
Whole Blood Samples

An ever-increasing need exists within the forensic laboratories to develop analytical processes for the qualitative and quantitative determination of a broad spectrum of new psychoactive substances. Phenylethylamine derivatives are among the major classes of psychoactive substances available on the global market and include both amphetamine analogues and synthetic cathinones. In this work, an ultra-high-performance liquid chromatography-positive ion electrospray ionization tandem mass spectrometric method (UHPLC-ESI-MS/MS) has been developed and fully validated for the determination of 19 psychoactive substances, including nine amphetamine-type stimulants and 10 synthetic cathinone derivatives, in premortem and postmortem whole blood. The assay was based on the use of 1 mL premortem or postmortem whole blood, following solid phase extraction prior to the analysis. The separation was achieved on a Poroshell 120 EC-C18 analytical column with a gradient mobile phase of 0.1% formic acid in acetonitrile and 0.1% formic acid in water in 9 min. The dynamic multiple reaction monitoring used in this work allowed for limit of detection (LOD) and lower limit of quantitation (LOQ) values of 0.5 and 2 ng mL−1, respectively, for all analytes both in premortem and postmortem whole blood samples. A quadratic calibration model was used for the 12 quantitative analytes over the concentration range of 20–2000 ng mL−1, and the method was shown to be precise and accurate both in premortem and postmortem whole blood. The method was applied to the analysis of real cases and proved to be a valuable tool in forensic and clinical toxicology.

Field analysis Cr(VI) in water samples by a smartphone-based ultralong absorption path reflection colorimetric device

2021 ◽  
Author(s):  
Xiaolan Chen ◽  
Cheng Ma ◽  
Qi Kang ◽  
Yu-Qin Chen ◽  
Dazhong Shen
Keyword(s):  
Hexavalent Chromium ◽  
Water Samples ◽  
Environmental Samples ◽  
Field Analysis ◽  
Absorption Path

Hexavalent chromium is highly toxic and regarded as one of the main pollutants in environmental samples. Sophisticated laboratory instruments are commercially available for in-field determination of chromium. It is highly...

New advances in the method validation, extraction methods and measurement uncertainty for the determination of water-soluble hexavalent chromium in hydraulic cement

Talanta ◽  
2021 ◽  
Vol 232 ◽  
pp. 122286
Author(s):  
María Melania Ramírez-Quesada ◽  
Jimmy Venegas-Padilla ◽  
José Pablo Sibaja-Brenes ◽  
Bryan Calderón-Jiménez
Keyword(s):  
Measurement Uncertainty ◽  
Hexavalent Chromium ◽  
Method Validation ◽  
Extraction Methods ◽  
Water Soluble ◽  
Hydraulic Cement
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