scholarly journals Partial Replacement with Menhaden Oil Improves Peripheral Neuropathy in High-Fat-Fed Low-Dose Streptozotocin Type 2 Diabetic Rat

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Lawrence J. Coppey ◽  
Amey Holmes ◽  
Eric P. Davidson ◽  
Mark A. Yorek
Keyword(s):  
Diabetic Neuropathy ◽  
High Fat Diet ◽  
Serum Lipid ◽  
Low Dose ◽  
Sensory Nerve ◽  
Diabetic Rats ◽  
Partial Replacement ◽  
Menhaden Oil ◽  
High Fat

Aims. To determine the effect of partial replacement of a high-fat diet with menhaden oil on diabetic neuropathy in an animal model of type 2 diabetes.Materials and Methods. High-fat/low-dose streptozotocin diabetic rats were used to examine the influence of replacing 50% of the source of the high-fat diet (lard) with menhaden oil, a natural source of n-3 fatty acids, on diabetic neuropathy. Endpoints included analyses of glucose tolerance, fatty liver disease, serum and liver fatty acid composition, serum lipid and adiponectin levels, motor and sensory nerve conduction velocity, thermal sensitivity and innervation of the hindpaw.Results. Diabetic rats were insulin resistant and menhaden oil did not improve whole animal glucose utilization. Menhaden oil did not improve elevated HbA1C levels or serum lipid levels but serum levels of adiponectin were significantly increased and hepatic steatosis was significantly improved. Diabetic rats were thermal hypoalgesic, had reduced motor and sensory nerve conduction velocities and intraepidermal nerve fiber profiles were decreased in the hindpaw and these endpoints were significantly improved with menhaden oil.Conclusions. We found that enrichment of a high-fat diet with menhaden oil improved a number of endpoints associated with diabetic neuropathy.

scholarly journals The Effect of Long-Term Intranasal Serotonin Treatment on Metabolic Parameters and Hormonal Signaling in Rats with High-Fat Diet/Low-Dose Streptozotocin-Induced Type 2 Diabetes

2015 ◽  
Vol 2015 ◽  
pp. 1-17 ◽  
Author(s):  
Kira V. Derkach ◽  
Vera M. Bondareva ◽  
Oxana V. Chistyakova ◽  
Lev M. Berstein ◽  
Alexander O. Shpakov
Keyword(s):  
Type 2 Diabetes ◽  
High Fat Diet ◽  
Low Dose ◽  
Diabetic Rats ◽  
Brain Serotonin ◽  
Serotonin Level ◽  
High Fat ◽  
Brain Serotonin Level ◽  
The Brain

In the last years the treatment of type 2 diabetes mellitus (DM2) was carried out using regulators of the brain signaling systems. In DM2 the level of the brain serotonin is reduced. So far, the effect of the increase of the brain serotonin level on DM2-induced metabolic and hormonal abnormalities has been studied scarcely. The present work was undertaken with the aim of filling this gap. DM2 was induced in male rats by 150-day high-fat diet and the treatment with low dose of streptozotocin (25 mg/kg) on the 70th day of experiment. From the 90th day, diabetic rats received for two months intranasal serotonin (IS) at a daily dose of 20 μg/rat. The IS treatment of diabetic rats decreased the body weight, and improved glucose tolerance, insulin-induced glucose utilization, and lipid metabolism. Besides, it restored hormonal regulation of adenylyl cyclase (AC) activity in the hypothalamus and normalized AC stimulation byβ-adrenergic agonists in the myocardium. In nondiabetic rats the same treatment induced metabolic and hormonal alterations, some of which were similar to those in DM2 but expressed to a lesser extent. In conclusion, the elevation of the brain serotonin level may be regarded as an effective approach to treat DM2 and its complications.

Antidiabetic effect of Ficus racemosa Linn. stem bark in high-fat diet and low-dose streptozotocin-induced type 2 diabetic rats: A mechanistic study

2012 ◽  
Vol 132 (1) ◽  
pp. 186-193 ◽  
Author(s):  
V.P. Veerapur ◽  
K.R. Prabhakar ◽  
B.S. Thippeswamy ◽  
Punit Bansal ◽  
K.K. Srinivasan ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Low Dose ◽  
Stem Bark ◽  
Diabetic Rats ◽  
Mechanistic Study ◽  
High Fat ◽  
Antidiabetic Effect ◽  
Ficus Racemosa ◽  
Type 2 Diabetic

Antidiabetic effects ofEmbelia ribesextract in high fat diet and low dose streptozotocin-induced type 2 diabetic rats

2013 ◽  
Vol 7 (3-4) ◽  
pp. 186-196 ◽  
Author(s):  
Uma Bhandari ◽  
Hemantkumar Somabhai Chaudhari ◽  
Geetika Khanna ◽  
Abul Kalam Najmi
Keyword(s):  
High Fat Diet ◽  
Low Dose ◽  
Diabetic Rats ◽  
High Fat ◽  
Type 2 Diabetic ◽  
Antidiabetic Effects

scholarly journals Comparative evaluation of the effect of Ocimum sanctum and metformin on serum lipid profile in high fat diet fed diabetic rats

2019 ◽  
Vol 8 (3) ◽  
pp. 589
Author(s):  
Shailendra Mishra ◽  
Quazi Shahir Ahmed ◽  
Kauser Sayedda
Keyword(s):  
Body Weight ◽  
High Fat Diet ◽  
Serum Lipid ◽  
Ethanolic Extract ◽  
Diabetic Rats ◽  
Ocimum Sanctum ◽  
Control Group ◽  
Lipid Levels ◽  
High Fat

Background: Dyslipidaemia is an important risk factor for development of macrovascular complications in type 2 diabetes mellitus. Ocimum sanctum (OS) and metformin have shown to have antihyperlipidaemic effects. The present study was undertaken  to evaluate the effects of  OS and Metformin on body weight & plasma lipid  levels of high fat diet fed diabetic ratsMethods: Total of 30 male wistar  rats (100-150gm) were obtained. Animals were fed with a high fat diet throughout the study (6 weeks). Diabetes was induced by using single intra-peritoneal injection of Streptozotocin 50mg/kg at the end of 4 weeks.  Diabetic rats were divided into groups of 6 each and treated as follows: Group 1- Diabetic control, was given vehicle orally. Group 2- O.S. ethanolic extract 100mg/kg body weight orally for 14 days. Group 3- O.S. ethanolic extract  200mg/kg body weight orally for 14 days. Group 4-  Metformin 100mg/day for 14 daysResults: At the end of 4 weeks, body weight of rats were significantly increased (p <0.05). Maximum weight gain was seen in control group whereas weight gain was least in O.S. 200mg/kg group (p >0.05). Decrease in body weight was seen in metformin group. Abdominal circumference of rats also showed similar pattern (p >0.05).  OS 200 caused significant reduction in serum LDL levels (p <0.05) and significant rise of serum HDL levels (p <0.05) as compared to control group. Metformin also favourably affected the lipid profile and its effects were not significantly different from effects of OS 200 (p> 0.05).Conclusions: Present study revealed that Ocimum Sanctum caused significant reduction in serum lipid levels in high fat diet fed diabetic rats. Metformin  also exhibited antihyperlipidaemic activity. So, it is concluded that OS or metformin alone or in combination  could be a novel adjunct to diet and life style modification for the management of dyslipidaemia in type 2 diabetes.  Further studies are required to confirm the antidyslipidaemic activities of individual phytoconstituents of Ocimum sanctum.

scholarly journals ANTIDIABETIC AND ANTIDYSLIPIDEMIC PROPERTIES OF GOA-111, A MIXTURE OF GYMNEMA SYLVESTRAE, OCIMUM SANCTUM AND AZADIRACHTA INDICA EXTRACT IN THE RATIO OF 1:1:1 STUDIED IN HIGH FAT DIET FED- LOW DOSE STREPTOZOTOCIN INDUCED EXPERIMENTAL TYPE 2 DIABETES IN RAT

2019 ◽  
Vol 9 (4-A) ◽  
pp. 115-121 ◽  
Author(s):  
Sangeetha Sathyanarayan ◽  
K. Sadasivan Pillai
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
High Fat Diet ◽  
Low Dose ◽  
Glycosylated Hemoglobin ◽  
Diabetic Rats ◽  
Ocimum Sanctum ◽  
High Fat ◽  
Experimental Type

Diabetes mellitus emerges from multiple biochemical and cellular impairments, including decreased insulin secretion from the pancreatic β-cells and impaired insulin action in peripheral tissues. The present study was systematically carried out to evaluate antidiabetic and  antidyslipidemic properties of GOA-111,a herbal extract containing a mixture of Gymnema sylvestrae, Ocimum sanctum leaves and seed kernel of Azadirachta indica  in the ratio of 1:1:1 in ameliorating both the primary and secondary complications of type 2 diabetes mellitus in high fat diet fed low dose streptozotocin induced diabetic rats Experimental type 2 diabetes was induced with a low dose streptozotocin in rats  fed on a high fat diet. Diabetic rats were treated with three different doses GOA 111  (150,300 and 450 mg/Kg b.wt/rat/day)   for 30 days. The toxicological parameters such as AST, ALT and ALP were assayed. Biochemical parameters such as fasting blood glucose, glycosylated hemoglobin, insulin, insulin resistance and lipid profile were measured. Oral treatment with GOA 111 significantly decreased the elevated levels of fasting glucose, glycosylated hemoglobin, AST, ALT and ALP. The insulin level was improved in insulin resistant diabetic rats.  GOA 111 also normalized the lipid profile. Though the  results showed a dose dependent impact on the parameters, a dose of  300mg/Kg B/W/rat/day GOA 111 exerts maximum potential anti-hyperglycemic and antidyslipidemic effects in HFD/STZ-induced type 2 diabetic rats. Key words: GOA 111; High fat diet; Streptozotocin; antidiabetic; antidyslipidemic

scholarly journals Synthesis, Spectral Characterization, and Biochemical Evaluation of Antidiabetic Properties of a New Zinc-Diosmin Complex Studied in High Fat Diet Fed-Low Dose Streptozotocin Induced Experimental Type 2 Diabetes in Rats

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Veerasamy Gopalakrishnan ◽  
Subramanian Iyyam Pillai ◽  
Sorimuthu Pillai Subramanian
Keyword(s):  
Type 2 Diabetes ◽  
Glucose Homeostasis ◽  
High Fat Diet ◽  
Low Dose ◽  
Diabetic Rats ◽  
Spectral Studies ◽  
Oral Glucose ◽  
High Fat ◽  
Experimental Type

In view of the established antidiabetic properties of zinc, the present study was aimed at evaluating the hypoglycemic properties of a new zinc-diosmin complex in high fat diet fed-low dose streptozotocin induced experimental type 2 diabetes in rats. Zinc-diosmin complex was synthesized and characterized by various spectral studies. The complexation between zinc ions and diosmin was further evidenced by pH-potentiometric titrations and Job’s plot. Diabetic rats were orally treated with zinc-diosmin complex at a concentration of 20 mg/kg b.w./rat/day for 30 days. At the end of the experimental period, the rats were subjected to oral glucose tolerance test. In addition, HOMA-IR and various biochemical parameters related to glucose homeostasis were analyzed. Treatment with zinc-diosmin complex significantly improved the glucose homeostasis in diabetic rats. Treatment with zinc-diosmin complex significantly improved insulin sensitivity, at least in part, through enhancing protein metabolism and alteration in the levels of muscle and liver glycogen. The assay of clinical marker enzymes revealed the nontoxic nature of the complex. Determination of renal tissue markers such as blood urea and serum creatinine indicates the renoprotective nature of the complex. These findings suggest that zinc-diosmin complex is nontoxic and has complimentary potential to develop as an antihyperglycemic agent for the treatment of diabetes mellitus.

scholarly journals ALOE-EMODIN GLYCOSIDES AMELIORATE GLUCOSE UTILIZATION VIA INSULIN DOWNSTREAM REGULATORS: AN IN VIVO INVESTIGATION

2016 ◽  
pp. 191 ◽  
Author(s):  
Singaravelu Anand ◽  
Saravanababu C ◽  
Lakshmi Bs ◽  
Muthusamy Vs
Keyword(s):  
Type 2 Diabetes ◽  
Insulin Receptor ◽  
Carbohydrate Metabolism ◽  
High Fat Diet ◽  
Low Dose ◽  
Diabetic Rats ◽  
High Fat ◽  
Aloe Emodin

<p>ABSTRACT<br />Objective: Aloe-emodin glycosides (AEG) isolated from Cassia fistula stimulates glucose transport and glycogen storage through a phosphatidylinositol<br />3 kinase (PI3K)-dependent mechanism in L6 myotubes and inhibits adipocytes differentiation in 3T3L1 adipocytes was previously reported. This<br />study intended to investigate the insulin mimetic effect of AEG by in vivo method.<br />Methods: Male Wistar albino rats were randomly allocated into two groups and fed for a period of 3-week. The high-fat diet group animals were<br />injected with a low dose (35 mg/kg) of streptozotocin to induce Type-2 diabetes. The diabetic rats were then treated with low dose: 10 mg/kg and<br />high dose: 30 mg/kg for a period of 21-day. A dose-dependent decrease in fasting blood glucose, cholesterol, and triglycerides levels on treatment<br />with AEG. The carbohydrate metabolism in diabetic rats appeared to improve due to regulation in hepatic enzymes such as hexokinase, glucose-6phosphatase,<br />and fructose<br />1,6-bisphosphatase with a concomitant increase<br />in glycogen<br />content.<br />Results: AEG decreased lipid peroxidation and improved the antioxidant (enzymatic and nonenzymatic) levels in the liver of diabetic rats. Treatment<br />with AEG (30 mg/kg) augmented the phosphorylation of insulin downstream regulators such as insulin receptor beta, insulin receptor substrate 1,<br />PI3K, glucose transporter 4, glycogen synthase kinase 3 beta, and peroxisome proliferator activator receptor gamma in the skeletal muscle tissue of<br />the Type-2 diabetic rats compared to vehicle-treated diabetic rats.<br />Conclusion: The present results suggested that AEG could serve as an interesting candidate in the drug development for the management of diabetes.<br />Keywords: Aloe-emodin glycoside, Type-2 diabetes, High-fat diet/streptozotocin, Carbohydrate Metabolism, Glycogen, Antioxidant enzyme.</p>

Antidiabetic effect ofDodonaea viscosaaerial parts in high fat diet and low dose streptozotocin-induced type 2 diabetic rats: A mechanistic approach

2010 ◽  
Vol 48 (10) ◽  
pp. 1137-1148 ◽  
Author(s):  
V.P. Veerapur ◽  
K.R. Prabhakar ◽  
M.R. Kandadi ◽  
K.K. Srinivasan ◽  
M.K. Unnikrishnan
Keyword(s):  
High Fat Diet ◽  
Low Dose ◽  
Diabetic Rats ◽  
High Fat ◽  
Antidiabetic Effect ◽  
Mechanistic Approach ◽  
Type 2 Diabetic
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