scholarly journals A First Analysis of Metallome Biosignatures of Hyperthermophilic Archaea

Archaea ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Vyllinniskii Cameron ◽  
Christopher H. House ◽  
Susan L. Brantley
Keyword(s):  
Trace Metal ◽  
Pyrococcus Furiosus ◽  
Hyperthermophilic Archaea ◽  
Metal Concentrations ◽  
E Coli ◽  
Reverse Pattern ◽  
Icp Ms ◽  
Metal Contents ◽  
Mesophilic Bacterium ◽  
Aerobic And Anaerobic

To date, no experimental data has been reported for the metallome of hyperthermophilic microorganisms although their metal requirements for growth are known to be unique. Here, experiments were conducted to determine (i) cellular trace metal concentrations of the hyperthermophilic ArchaeaMethanococcus jannaschiiandPyrococcus furiosus, and (ii) a first estimate of the metallome for these hyperthermophilic species via ICP-MS. The metal contents of these cells were compared to parallel experiments using the mesophilic bacteriumEscherichia coligrown under aerobic and anaerobic conditions. Fe and Zn were typically the most abundant metals in cells. Metal concentrations forE. coligrown aerobically decreased in the order Fe > Zn > Cu > Mo > Ni > W > Co. In contrast,M. jannaschiiandP. furiosusshow almost the reverse pattern with elevated Ni, Co, and W concentrations. Of the three organisms, a biosignature is potentially demonstrated for the methanogenM. jannaschiithat may, in part, be related to the metallome requirements of methanogenesis. The bioavailability of trace metals more than likely has varied through time. If hyperthermophiles are very ancient, then the trace metal patterns observed here may begin to provide some insights regarding Earth's earliest cells and in turn, early Earth chemistry.

The impacts of seawater physicochemical parameters and sediment metal contents on trace metal concentrations in mussels—a chemometric approach

2018 ◽  
Vol 25 (28) ◽  
pp. 28248-28263 ◽  
Author(s):  
Ana Perošević ◽  
Lato Pezo ◽  
Danijela Joksimović ◽  
Dijana Đurović ◽  
Ivana Milašević ◽  
...  
Keyword(s):  
Trace Metal ◽  
Physicochemical Parameters ◽  
Metal Concentrations ◽  
Metal Contents ◽  
Chemometric Approach ◽  
Sediment Metal

scholarly journals Rubrerythrin from the Hyperthermophilic Archaeon Pyrococcus furiosus Is a Rubredoxin-Dependent, Iron-Containing Peroxidase

2004 ◽  
Vol 186 (23) ◽  
pp. 7888-7895 ◽  
Author(s):  
Michael V. Weinberg ◽  
Francis E. Jenney ◽  
Xiaoyuan Cui ◽  
Michael W. W. Adams
Keyword(s):  
Hydrogen Peroxide ◽  
Electron Donor ◽  
Metal Content ◽  
Specific Activity ◽  
Pyrococcus Furiosus ◽  
Hyperthermophilic Archaeon ◽  
E Coli ◽  
Reducing Conditions ◽  
Metal Contents ◽  
Protein Functions

ABSTRACT Rubrerythrin was purified by multistep chromatography under anaerobic, reducing conditions from the hyperthermophilic archaeon Pyrococcus furiosus. It is a homodimer with a molecular mass of 39.2 kDa and contains 2.9 ± 0.2 iron atoms per subunit. The purified protein had peroxidase activity at 85°C using hydrogen peroxide with reduced P. furiosus rubredoxin as the electron donor. The specific activity was 36 μmol of rubredoxin oxidized/min/mg with apparent Km values of 35 and 70 μM for hydrogen peroxide and rubredoxin, respectively. When rubrerythrin was combined with rubredoxin and P. furiosus NADH:rubredoxin oxidoreductase, the complete system used NADH as the electron donor to reduce hydrogen peroxide with a specific activity of 7.0 μmol of H2O2 reduced/min/mg of rubrerythrin at 85°C. Strangely, as-purified (reduced) rubrerythrin precipitated when oxidized by either hydrogen peroxide, air, or ferricyanide. The gene (PF1283) encoding rubrerythrin was expressed in Escherichia coli grown in medium with various metal contents. The purified recombinant proteins each contained approximately three metal atoms/subunit, ranging from 0.4 Fe plus 2.2 Zn to 1.9 Fe plus 1.2 Zn, where the metal content of the protein depended on the metal content of the E. coli growth medium. The peroxidase activities of the recombinant forms were proportional to the iron content. P. furiosus rubrerythrin is the first to be characterized from a hyperthermophile or from an archaeon, and the results are the first demonstration that this protein functions in an NADH-dependent, hydrogen peroxide:rubredoxin oxidoreductase system. Rubrerythrin is proposed to play a role in the recently defined anaerobic detoxification pathway for reactive oxygen species.

scholarly journals Characterization of Ten Heterotetrameric NDP-Dependent Acyl-CoA Synthetases of the Hyperthermophilic ArchaeonPyrococcus furiosus

Archaea ◽  
2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Joseph W. Scott ◽  
Farris L. Poole ◽  
Michael W. W. Adams
Keyword(s):  
Amino Acids ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Pyrococcus Furiosus ◽  
Hyperthermophilic Archaea ◽  
E Coli ◽  
Proteomic Data ◽  
Genes Encoding ◽  
Derivatives Of ◽  
Dependent Processes

The hyperthermophilic archaeonPyrococcus furiosusgrows by fermenting peptides and carbohydrates to organic acids. In the terminal step, acyl-CoA synthetase (ACS) isoenzymes convert acyl-CoA derivatives to the corresponding acid and conserve energy in the form of ATP. ACS1 and ACS2 were previously purified fromP. furiosusand haveα2β2structures but the genome contains genes encoding three additionalα-subunits. The ten possible combinations ofαandβgenes were expressed inE. coliand each resulted in stable and activeα2β2isoenzymes. Theα-subunit of each isoenzyme determined CoA-based substrate specificity and between them they accounted for the CoA derivatives of fourteen amino acids. Theβ-subunit determined preference for adenine or guanine nucleotides. The GTP-generating isoenzymes are proposed to play a role in gluconeogenesis by producing GTP for GTP-dependent phosphoenolpyruvate carboxykinase and for other GTP-dependent processes. Transcriptional and proteomic data showed that all ten isoenzymes are constitutively expressed indicating that both ATP and GTP are generated from the metabolism of most of the amino acids. A phylogenetic analysis showed that the ACSs ofP. furiosusand other members of the Thermococcales are evolutionarily distinct from those found throughout the rest of biology, including those of other hyperthermophilic archaea.

scholarly journals Inputs of Total and Labile Dissolved Metals from Six Facilities Continuously Discharging Treated Wastewaters to the Marine Environment of Gran Canaria Island (Canary Islands, Spain)

2021 ◽  
Vol 18 (21) ◽  
pp. 11582
Author(s):  
Marta Rodrigo Sanz ◽  
Vanessa Millán Gabet ◽  
Jean-Louis Gonzalez
Keyword(s):  
Wastewater Treatment Plants ◽  
Treated Wastewater ◽  
Dissolved Metals ◽  
Metal Concentrations ◽  
Efficient Treatment ◽  
Gran Canaria ◽  
Sampling Campaign ◽  
Icp Ms ◽  
Metal Contents ◽  
Thin Film Technique

The presence of ten metals (Cd, Ni, Pb, Cr, Cu, Zn, Al, Fe, Mn, and Co) was investigated in the final discharge of six facilities, including four wastewater treatment plants, which were continuously discharging treated wastewater to the coastal environment in Gran Canaria Island. A four-day sampling campaign was carried out at each facility in July 2020, in which both the spot samplings technique and the diffusive gradient in thin-film technique (DGT) were carried out to measure total dissolved metals and the in situ labile metal fraction, respectively. After the necessary sample preparation steps, measurements were carried out by ICP-MS for both samplings. Raw data referred to the spot total dissolved and DGT-labile metal concentrations were reported. In general, the average metal concentrations were dispersed in a broad range. As expected, the highest metal contents were found in those facilities with larger industrial contributions. The values of annual average environmental quality standards (AA-EQS) were used to assess the total dissolved metal concentrations for every metal in every final discharge. In only one of the studied facilities, some metals (Ni and Zn) exceeded these EQS within the receiving waterbody, highlighting the need for more efficient treatment targeted towards a specific discharging-water quality. In addition, the total dissolved and labile metal daily fluxes of discharge were calculated to estimate the contribution of every effluent to the receiving water bodies.

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