scholarly journals Raman Microspectroscopy of the Yeast Vacuoles

2012 ◽  
Vol 27 ◽  
pp. 503-507 ◽  
Author(s):  
Lucie Bednárová ◽  
Jan Palacký ◽  
Václava Bauerová ◽  
Olga Hrušková-Heidingsfeldová ◽  
Iva Pichová ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Immobilized Cells ◽  
Raman Microspectroscopy ◽  
Stress Factors ◽  
Yeast Cells ◽  
Multivariate Methods ◽  
Label Free ◽  
Spatially Resolved ◽  
Confocal Raman Microspectroscopy ◽  
Yeast Immobilization

In the present work, real ability of a confocal Raman microspectroscopy to monitor chemical composition of the vacuoles within living yeast cells was investigated and critically assessed. Simple, economical, and practical protocols of the yeast immobilization suitable for less laborious, high-throughput, and spatially resolved Raman measurements were tested for their possible impacts on physiological states and viability of the cells. We have demonstrated that, acquiring Raman spectra from statistically sound sets of immobilized cells and employing advanced multivariate methods for spectral analysis, the chemical composition of the yeast vacuoles can be reliably studied. The most easily and accurately quantifiable seems to be the concentration of polyphosphates which can be unambiguously identified due to unmistakable Raman features. Our approach can be useful for routine, label-free, and noninvasive monitoring of the chemical composition of the vacuoles of living yeasts exposed to various stress factors, the information important in biomedical research of pathogens.

Label-Free Mapping of the Biochemicals in Tomato Fruit by Confocal Raman Microspectroscopy

2019 ◽  
Vol 42 (4) ◽  
pp. 365-368 ◽  
Author(s):  
Sweta Sharma ◽  
Rahul Uttam ◽  
Abhi Sarika Bharti ◽  
Nidhi Shukla ◽  
K. N. Uttam
Keyword(s):  
Tomato Fruit ◽  
Raman Microspectroscopy ◽  
Label Free ◽  
Confocal Raman Microspectroscopy ◽  
Confocal Raman

scholarly journals Tear Down the Fluorescent Curtain: A New Fluorescence Suppression Method for Raman Microspectroscopic Analyses

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Elena Yakubovskaya ◽  
Tatiana Zaliznyak ◽  
Joaquin Martínez Martínez ◽  
Gordon T. Taylor
Keyword(s):  
Biological Samples ◽  
Raman Microspectroscopy ◽  
Peroxide Oxidation ◽  
Isotopic Tracers ◽  
New Approach ◽  
Viral Propagation ◽  
Spatially Resolved ◽  
Confocal Raman Microspectroscopy ◽  
Suppression Method ◽  
Indispensable Tool

Abstract The near exponential proliferation of published Raman microspectroscopic applications over the last decade bears witness to the strengths and versatility of this technology. However, laser-induced fluorescence often severely impedes its application to biological samples. Here we report a new approach for near complete elimination of laser-induced background fluorescence in highly pigmented biological specimens (e.g., microalgae) enabling interrogation by Raman microspectroscopy. Our simple chemiphotobleaching method combines mild hydrogen peroxide oxidation with broad spectrum visible light irradiation of the entire specimen. This treatment permits observing intracellular distributions of macromolecular pools, isotopic tracers, and even viral propagation within cells previously not amenable to Raman microspectroscopic examination. Our approach demonstrates the potential for confocal Raman microspectroscopy becoming an indispensable tool to obtain spatially-resolved data on the chemical composition of highly fluorescent biological samples from individual cells to environmental samples.

Multidimensional Information on the Chemical Composition of Single Bacterial Cells by Confocal Raman Microspectroscopy

2000 ◽  
Vol 72 (22) ◽  
pp. 5529-5534 ◽  
Author(s):  
K. Christian Schuster ◽  
Ingo Reese ◽  
Eva Urlaub ◽  
J. Richard Gapes ◽  
Bernhard Lendl
Keyword(s):  
Chemical Composition ◽  
Raman Microspectroscopy ◽  
Bacterial Cells ◽  
Confocal Raman Microspectroscopy ◽  
Multidimensional Information ◽  
Confocal Raman

scholarly journals Cultivation conditions preferable for yeast cells to be immobilized into poly(vinyl alcohol) and used in bottled sparkling wine production

2006 ◽  
Vol 12 (1) ◽  
pp. 18-23 ◽  
Author(s):  
Elena Efremenko ◽  
Nikolay Stepanov ◽  
Nikolay Martinenko ◽  
Irina Gracheva
Keyword(s):  
Vinyl Alcohol ◽  
Immobilized Cells ◽  
Yeast Cells ◽  
Poly Vinyl Alcohol ◽  
Sparkling Wine ◽  
Wine Production ◽  
Biochemical Status ◽  
Viability Level ◽  
Yeast Immobilization ◽  
High Level

An effective approach to the improvement of the biochemical status of yeast cells before their immobilization was applied to develop a biocatalyst with a high level of cell metabolic activity and viability that could be used for sparkling wine production. According to the approach, the wine-containing medium (pH 3.0) routinely used for the accumulation of champagne yeast biomass under aerobic conditions was replaced by a semi-synthetic one (pH 5.6), usually applied for aerobic yeast growth. The variation of temperature and pH conditions of cell growth showed a modification of the fatty acid pool of yeasts and its importance for the further immobilization of cells into a poly(vinyl alcohol) cryogel. Measurement of the specific intracellular ATP concentration by the bioluminescent method revealed the growth phase favorable for yeast immobilization. The main characteristics of the sparkling wine obtained after four-week fermentation with application of both the free and immobilized cells were similar but, according to the detected energetic status, the viability level of the immobilized cells was considerably higher compared to the free yeast. The CO2 pressure accumulated in the bottles with immobilized cells (up to 500 kPa) after fermentation also appeared notably higher than in the bottles with free yeast.

Label-Free Determination of the Cell Cycle Phase in Human Embryonic Stem Cells by Raman Microspectroscopy

2013 ◽  
Vol 85 (19) ◽  
pp. 8996-9002 ◽  
Author(s):  
Stanislav O. Konorov ◽  
H. Georg Schulze ◽  
James M. Piret ◽  
Michael W. Blades ◽  
Robin F. B. Turner
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Raman Microspectroscopy ◽  
Cell Cycle Phase ◽  
Cycle Phase ◽  
Label Free
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