scholarly journals Overexpression of PGC-1αIncreases Fatty Acid Oxidative Capacity of Human Skeletal Muscle Cells

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Nataša Nikolić ◽  
Magdalena Rhedin ◽  
Arild C. Rustan ◽  
Len Storlien ◽  
G. Hege Thoresen ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Lipid Metabolism ◽  
Mrna Expression ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Oxidative Capacity ◽  
Skeletal Muscle Cells ◽  
Expression Of Genes ◽  
Human Myotubes ◽  
Human Skeletal Muscle Cells

We investigated the effects of PGC-1α(peroxisome proliferator-activated receptorγcoactivator-1α) overexpression on the oxidative capacity of human skeletal muscle cellsex vivo. PGC-1αoverexpression increased the oxidation rate of palmitic acid and mRNA expression of genes regulating lipid metabolism, mitochondrial biogenesis, and function in human myotubes. Basal and insulin-stimulated deoxyglucose uptake were decreased, possibly due to upregulation of PDK4 mRNA. Expression of fast fiber-type gene marker (MHCIIa) was decreased. Compared to skeletal musclein vivo, PGC-1αoverexpression increased expression of several genes, which were downregulated during the process of cell isolation and culturing. In conclusion, PGC-1αoverexpression increased oxidative capacity of cultured myotubes by improving lipid metabolism, increasing expression of genes involved in regulation of mitochondrial function and biogenesis, and decreasing expression of MHCIIa. These results suggest that therapies aimed at increasing PGC-1αexpression may have utility in treatment of obesity and obesity-related diseases.

scholarly journals Erratum to “Overexpression of PGC-1Increases Fatty Acid Oxidative Capacity of Human Skeletal Muscle Cells”

2013 ◽  
Vol 2013 ◽  
pp. 1-2
Author(s):  
Nataša Nikolić ◽  
Magdalena Rhedin ◽  
Arild C. Rustan ◽  
Len Storlien ◽  
G. Hege Thoresen ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Fatty Acid ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Oxidative Capacity ◽  
Skeletal Muscle Cells ◽  
Human Skeletal Muscle Cells

Lipid metabolism in human skeletal muscle cells: effects of palmitate and chronic hyperglycaemia

2005 ◽  
Vol 183 (1) ◽  
pp. 31-41 ◽  
Author(s):  
V. Aas ◽  
M. Rokling-Andersen ◽  
A. J. Wensaas ◽  
G. H. Thoresen ◽  
E. T. Kase ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Lipid Metabolism ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Human Skeletal Muscle Cells ◽  
Chronic Hyperglycaemia

Proteomic identification of secreted proteins from human skeletal muscle cells and expression in response to strength training

2011 ◽  
Vol 301 (5) ◽  
pp. E1013-E1021 ◽  
Author(s):  
Frode Norheim ◽  
Truls Raastad ◽  
Bernd Thiede ◽  
Arild C. Rustan ◽  
Christian A. Drevon ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Strength Training ◽  
Skeletal Muscles ◽  
Human Skeletal Muscle ◽  
Vastus Lateralis ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Secreted Proteins ◽  
Human Myotubes ◽  
Human Skeletal Muscle Cells

Regular physical activity protects against several types of diseases. This may involve altered secretion of signaling proteins from skeletal muscle. Our aim was to identify the most abundantly secreted proteins in cultures of human skeletal muscle cells and to monitor their expression in muscles of strength-training individuals. A total of 236 proteins were detected by proteome analysis in medium conditioned by cultured human myotubes, which was narrowed down to identification of 18 classically secreted proteins expressed in skeletal muscle, using the SignalP 3.0 and Human Genome Expression Profile databases together with a published mRNA-based reconstruction of the human skeletal muscle secretome. For 17 of the secreted proteins, expression was confirmed at the mRNA level in cultured human myotubes as well as in biopsies of human skeletal muscles. RT-PCR analyses showed that 15 of the secreted muscle proteins had significantly enhanced mRNA expression in m. vastus lateralis and/or m. trapezius after 11 wk of strength training among healthy volunteers. For example, secreted protein acidic and rich in cysteine, a secretory protein in the membrane fraction of skeletal muscle fibers, was increased 3- and 10-fold in m. vastus lateralis and m. trapezius, respectively. Identification of proteins secreted by skeletal muscle cells in vitro facilitated the discovery of novel responses in skeletal muscles of strength-training individuals.

scholarly journals Annexin-A6 in Membrane Repair of Human Skeletal Muscle Cell: A Role in the Cap Subdomain

Cells ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 1742 ◽  
Author(s):  
Coralie Croissant ◽  
Céline Gounou ◽  
Flora Bouvet ◽  
Sisareuth Tan ◽  
Anthony Bouter
Keyword(s):  
Electron Microscopy ◽  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Membrane Repair ◽  
Membrane Injury ◽  
Human Myotubes ◽  
Human Skeletal Muscle Cells ◽  
Membrane Resealing

Defects in membrane repair contribute to the development of some muscular dystrophies, highlighting the importance to decipher the membrane repair mechanisms in human skeletal muscle. In murine myofibers, the formation of a cap subdomain composed notably by annexins (Anx) is critical for membrane repair. We applied membrane damage by laser ablation to human skeletal muscle cells and assessed the behavior of annexin-A6 (AnxA6) tagged with GFP by correlative light and electron microscopy (CLEM). We show that AnxA6 was recruited to the site of membrane injury within a few seconds after membrane injury. In addition, we show that the deficiency in AnxA6 compromises human sarcolemma repair, demonstrating the crucial role played by AnxA6 in this process. An AnxA6-containing cap-subdomain was formed in damaged human myotubes in about one minute. Through transmission electron microscopy (TEM), we observed that extension of the sarcolemma occurred during membrane resealing, which participated in forming a dense lipid structure in order to plug the hole. By properties of membrane folding and curvature, AnxA6 helped in the formation of this tight structure. The compaction of intracellular membranes—which are used for membrane resealing and engulfed in extensions of the sarcolemma—may also facilitate elimination of the excess of lipid and protein material once cell membrane has been repaired. These data reinforce the role played by AnxA6 and the cap subdomain in membrane repair of skeletal muscle cells.

scholarly journals Temporal analysis of reciprocal miRNA-mRNA expression patterns predicts regulatory networks during differentiation in human skeletal muscle cells

2015 ◽  
Vol 47 (3) ◽  
pp. 45-57 ◽  
Author(s):  
Rasmus J. O. Sjögren ◽  
Brendan Egan ◽  
Mutsumi Katayama ◽  
Juleen R. Zierath ◽  
Anna Krook
Keyword(s):  
Skeletal Muscle ◽  
Mrna Expression ◽  
Human Skeletal Muscle ◽  
Expression Patterns ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Altered Expression ◽  
Human Skeletal Muscle Cells ◽  
Microarray Profiling ◽  
Reciprocal Expression

microRNAs (miRNAs) are short noncoding RNAs that regulate gene expression through posttranscriptional repression of target genes. miRNAs exert a fundamental level of control over many developmental processes, but their role in the differentiation and development of skeletal muscle from myogenic progenitor cells in humans remains incompletely understood. Using primary cultures established from human skeletal muscle satellite cells, we performed microarray profiling of miRNA expression during differentiation of myoblasts ( day 0) into myotubes at 48 h intervals ( day 2, 4, 6, 8, and 10). Based on a time-course analysis, we identified 44 miRNAs with altered expression [false discovery rate (FDR) < 5%, fold change > ±1.2] during differentiation, including the marked upregulation of the canonical myogenic miRNAs miR-1, miR-133a, miR-133b, and miR-206. Microarray profiling of mRNA expression at day 0, 4, and 10 identified 842 and 949 genes differentially expressed (FDR < 10%) at day 4 and 10, respectively. At day 10, 42% of altered transcripts demonstrated reciprocal expression patterns in relation to the directional change of their in silico predicted regulatory miRNAs based on analysis using Ingenuity Pathway Analysis microRNA Target Filter. Bioinformatic analysis predicted networks of regulation during differentiation including myomiRs miR-1/206 and miR-133a/b, miRNAs previously established in differentiation including miR-26 and miR-30, and novel miRNAs regulated during differentiation of human skeletal muscle cells such as miR-138-5p and miR-20a. These reciprocal expression patterns may represent new regulatory nodes in human skeletal muscle cell differentiation. This analysis serves as a reference point for future studies of human skeletal muscle differentiation and development in healthy and disease states.

TGFß regulates metabolism of human skeletal muscle cells by miRNAs

2018 ◽  
Author(s):  
S Höckele ◽  
P Huypens ◽  
C Hoffmann ◽  
T Jeske ◽  
M Hastreiter ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Human Skeletal Muscle Cells

Telomerase Alone Extends the Replicative Life Span of Human Skeletal Muscle Cells Without Compromising Genomic Stability

2003 ◽  
Vol 14 (15) ◽  
pp. 1473-1487 ◽  
Author(s):  
Martha Wootton ◽  
Karen Steeghs ◽  
Diana Watt ◽  
June Munro ◽  
Katrina Gordon ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Life Span ◽  
Human Skeletal Muscle ◽  
Genomic Stability ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Replicative Life Span ◽  
Human Skeletal Muscle Cells
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