scholarly journals Application of Serial Analysis of Gene Expression to the Study of the Gene Expression Profile ofLeishmania infantum chagasiPromastigote

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Adelino Soares Lima Neto ◽  
Osvaldo Pompílio de Melo Neto ◽  
Carlos Henrique Nery Costa
Keyword(s):  
Gene Expression ◽  
Life Cycle ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Leishmania Infantum ◽  
Open Reading Frames ◽  
Genomic Sequences ◽  
Coding Sequences ◽  
Key Genes ◽  
Reading Frames

This study describes the application of the LongSAGE methodology to study the gene expression profile in promastigotes ofLeishmania infantum chagasi. A tag library was created using the LongSAGE method and consisted of 14,208 tags of 17 bases. Of these, 8,427 (59.3%) were distinct. BLAST research of the 1,645 most abundant tags showed that 12.8% of them identified the coding sequences of genes, while 82% (1,349/1,645) identified one or more genomic sequences that did not correspond with open reading frames. Only 5.2% (84/1,645) of the tags were not aligned to any position in theL. infantum genome. The UTR size ofLeishmaniaand the lack of CATG sites in some transcripts were decisive for the generation of tags in these regions. Additional analysis will allow a better understanding of the expression profile and discovering the key genes in this life cycle.

scholarly journals Anaplasma phagocytophilumandAnaplasma marginaleElicit Different Gene Expression Responses in Cultured Tick Cells

2009 ◽  
Vol 2009 ◽  
pp. 1-9 ◽  
Author(s):  
Zorica Zivkovic ◽  
Edmour F. Blouin ◽  
Raúl Manzano-Roman ◽  
Consuelo Almazán ◽  
Victoria Naranjo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Life Cycle ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Anaplasma Phagocytophilum ◽  
Ixodes Scapularis ◽  
Host Cells ◽  
Rt Pcr ◽  
Infected Tick ◽  
Tick Survival

The genusAnaplasma(Rickettsiales: Anaplasmataceae) includes obligate tick-transmitted intracellular organisms,Anaplasma phagocytophilumandAnaplasma marginalethat multiply in both vertebrate and tick host cells. Recently, we showed thatA. marginaleaffects the expression of tick genes that are involved in tick survival and pathogen infection and multiplication. However, the gene expression profile inA. phagocytophilum-infected tick cells is currently poorly characterized. The objectives of this study were to characterize tick gene expression profile inIxodes scapularisticks and cultured ISE6 cells in response to infection withA. phagocypthilumand to compare tick gene expression responses inA. phagocytophilum- andA. marginale-infected tick cells by microarray and real-time RT-PCR analyses. The results of these studies demonstrated modulation of tick gene expression byA. phagocytophilumand provided evidence of different gene expression responses in tick cells infected withA. phagocytophilumandA. marginale. These differences inAnaplasma-tick interactions may reflect differences in pathogen life cycle in the tick cells.

scholarly journals Identification of potential key genes associated with glioblastoma based on the gene expression profile

2017 ◽  
Vol 14 (2) ◽  
pp. 2045-2052 ◽  
Author(s):  
Lijuan Bo ◽  
Bo Wei ◽  
Chaohui Li ◽  
Zhanfeng Wang ◽  
Zheng Gao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Key Genes

scholarly journals Gene expression profile during the life cycle of the urochordate Ciona intestinalis

2007 ◽  
Vol 308 (2) ◽  
pp. 572-582 ◽  
Author(s):  
Kaoru Azumi ◽  
Sorin V. Sabau ◽  
Manabu Fujie ◽  
Takeshi Usami ◽  
Ryo Koyanagi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Life Cycle ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Ciona Intestinalis
Sign in / Sign up

Export Citation Format

Share Document