scholarly journals Management of Root Perforations Using MTA with or without Er:YAG Laser Irradiation: An In Vitro Study

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
M. Tielemans ◽  
I. Saloukas ◽  
D. Heysselaer ◽  
Ph. Compere ◽  
C. Nyssen-Behets ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Laser Irradiation ◽  
Er:Yag Laser ◽  
Mineral Trioxide Aggregate ◽  
In Vitro Study ◽  
Vitro Study ◽  
Blue Dye ◽  
Beam Diameter ◽  
Significant Difference

The aim of this in vitro study is to compare the microleakage of a root perforation sealed with MTA (mineral trioxide aggregate) (group M) to that sealed with MTA following Er:YAG laser irradiation (group ML). Forty-two recently extracted human monoroot teeth were used. Two cavities were prepared on each root surface. Randomly, on each root, the exposed dentine of one cavity was irradiated prior to MTA filling using an Er:YAG laser with the following settings: 200 mJ/pulses under an air water spray, 10 Hz, pulse duration of 50 μsec, and 0.7 mm beam diameter. All cavities were then sealed with MTA. submitted to thermocycling and immersed in 2% methylene blue dye solution for 12 h. The penetration of methylene blue in the microleakage of cavity was observed and recorded. The mean value dye penetration in cavities sealed with MTA following Er:YAG laser irradiation (%) was lower than that of unlased cavities sealed only with MTA (%). No significant difference was noted. The use of an Er:YAG laser beam for dentinal conditioning prior to MTA filling of perforated roots did not decrease significantly the microleakage of MTA sealing when compared to the conventional use of MTA filling.

scholarly journals Er:YAG laser-induced damage to a dental composite in simulated clinical scenarios for inadvertent irradiation: an in vitro study

2021 ◽  
Author(s):  
Katharina Kuhn ◽  
Carmen U. Schmid ◽  
Ralph G. Luthardt ◽  
Heike Rudolph ◽  
Rolf Diebolder
Keyword(s):  
Laser Irradiation ◽  
Laser Fluence ◽  
Ablation Threshold ◽  
Er:Yag Laser ◽  
In Vitro Study ◽  
Vitro Study ◽  
Crater Diameter ◽  
Clinical Scenarios ◽  
Laser Induced Damage

AbstractInadvertent Er:YAG laser irradiation occurs in dentistry and may harm restorative materials in teeth. The aim of this in vitro study was to quantify Er:YAG laser-induced damage to a nanohybrid composite in simulated clinical scenarios for inadvertent direct and indirect (reflection) laser irradiation. The simulation was performed by varying the output energy (OE;direct˃indirect) reaching the specimen and the operating distance (OD;direct˂indirect). Composite specimens were irradiated by an Er:YAG laser. The ablation threshold was determined and clinically relevant parameters were applied (n = 6 for each OE/OD combination) for direct (OE: 570 mJ/OD: 10 mm, OE: 190 mJ/OD: 10 mm) and indirect irradiation (OE: 466 mJ/OD: 15 mm, OE: 57 mJ/OD: 15 mm, OE: 155 mJ/OD: 15 mm, OE: 19 mJ/OD: 15 mm). The extent of damage in the form of craters was evaluated using a laser scanning microscope (LSM) and a conventional light microscope (LM). The ablation threshold was determined to be 2.6 J/cm2. The crater diameter showed the highest value (LM: 1075 ± 18 µm/LSM: 1082 ± 17 µm) for indirect irradiation (reflectant:dental mirror) (OE: 466 mJ/OD: 15 mm). The crater depth showed the highest and comparable value for direct (OE: 570 mJ/OD: 10 mm; LSM: 89 ± 2 µm) and indirect irradiation (OE: 466 mJ/OD: 15 mm; LSM: 90 ± 4 µm). For each OD, the crater diameter, depth, and volume increased with higher laser fluence. However, the OD—and thus the laser spot diameter—also had an enlarging effect. Thus, indirect irradiation (reflectant:dental mirror) with only 47% of the laser fluence of direct irradiation led to a larger diameter and a comparable depth. The three-dimensional extent of the crater was large enough to cause roughening, which may lead to plaque accumulation and encourage caries, gingivitis, and periodontitis under clinical conditions. Clinicians should be aware that reflected irradiation can still create such craters.

Effect of Er:YAG laser irradiation on deciduous enamel roughness and bacterial adhesion: An in vitro study

2019 ◽  
Author(s):  
Bernardo Teutle‐Coyotecatl ◽  
Rosalía Contreras‐Bulnes ◽  
Rogelio José Scougall‐Vilchis ◽  
Argelia Almaguer‐Flores ◽  
Laura Emma Rodríguez‐Vilchis ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Bacterial Adhesion ◽  
Er:Yag Laser ◽  
In Vitro Study ◽  
Vitro Study ◽  
Enamel Roughness

scholarly journals Comparison of MTA, CEM Cement, and Biodentine as Coronal Plug during Internal Bleaching: An In Vitro Study

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Paridokht Zarean ◽  
Parichehr Zarean ◽  
Arash Ravaghi ◽  
Maryam Zare Jahromi ◽  
Mitra Sadrameli
Keyword(s):  
Root Resorption ◽  
Mineral Trioxide Aggregate ◽  
In Vitro Study ◽  
Negative Control ◽  
Vitro Study ◽  
Control Group ◽  
Endodontically Treated Teeth ◽  
Cem Cement ◽  
Significant Difference

Background. Internal bleaching is a choice of treatment in discolored endodontically treated teeth. Cervical root resorption is one of the important complications of this treatment. A suggested procedure to prevent this type of resorption is using a coronal barrier under the bleaching materials. The aim of the study was to compare the microleakage of mineral trioxide aggregate (MTA), calcium-enriched mixture (CEM) cement, and Biodentine. Materials and Methods. In this in vitro study, a total of 60 single canal incisors were included. They were randomly divided into three experimental groups (n = 16), one positive control group (n = 6), and one negative control group (n = 6). Coronal portion of the canals in the experimental groups was sealed with 3 mm of MTA, CEM cement, or Biodentine as a coronal barrier. After 3 days, specimens were bleached. A fresh Enterococcus faecalis suspension was added to the samples. The culture tubes were observed for 45 days, and the daily turbidity was recorded. Statistical analysis was accomplished by the Kaplan–Meier test and SPSS 22. Results. All positive samples showed turbidity, whereas none of the negative samples allowed bacterial leakage. Results showed no significant difference between MTA, CEM cement, and Biodentine groups. ( P value = 0.304, 0.695, and 0.217). The bacterial microleakage for the two groups also did not show significant differences. Conclusions. CEM cement and Biodentine showed promising results as coronal plug, and clinical studies are needed to test these materials with MTA for avoiding microleakage in internal bleaching treatment.

scholarly journals Assessment of Exogenous Pigmentation on Clear Elastomeric Modules: An in vitro Study

2018 ◽  
Vol 8 (1) ◽  
pp. 33-36
Author(s):  
Shaista N Ansari ◽  
Dhananjay Rathod ◽  
G P Rathod ◽  
Gazal Bisht
Keyword(s):  
Methylene Blue ◽  
In Vitro Study ◽  
Vitro Study ◽  
Orthodontic Appliances ◽  
Significant Difference ◽  
Adobe Photoshop ◽  
Group A ◽  
Strong Staining ◽  
Group B

Objective: To assess in vitro changes in the shade of clear elastomeric modules from different manufacturers influenced by exogeneous pigmentation contained in everyday dietary substances.Materials & Method: The specimen comprised of clear elastomeric modules (Group A: Libral, Group B: TP Orthodontics) which were immersed in dietary substances: ketchup, coffee, tea, pepsi & methylene blue. Their hue, saturation and intensity were figured after 72 hours by methods of Adobe photoshop CS3 to mirror the module recoloring seriousness.Result: Significant difference was found between two types of modules analyzed in this study. Ketchup and methylene blue demonstrated higher staining potential compared to other staining media. Besides, there was no significant difference in staining contrast between tea and Pepsi.Conclusion: Ketchup and methylene blue are strong staining media, ought to be avoided by the patients opting for aesthetic orthodontic appliances. Elastomeric modules manufactured by different companies have differing staining potential. 

Antimicrobial effect of Er:YAG laser irradiation in infected root canals: an in vitro study

2003 ◽  
Author(s):  
Marcelo Sampaio Moura ◽  
Fatima A. A. Zanin ◽  
Aldo Brugnera, Jr. ◽  
Cyntia Rodrigues de Araujo Estrela ◽  
Carlos Estrela ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Er:Yag Laser ◽  
In Vitro Study ◽  
Antimicrobial Effect ◽  
Vitro Study ◽  
Root Canals ◽  
Infected Root

scholarly journals An In-vitro Study to Determine the Sealing Ability of Biodentine when used as a Class II Restorative Material

2020 ◽  
Author(s):  
Deebah Choudhary
Keyword(s):  
Base Material ◽  
In Vitro Study ◽  
Class Ii ◽  
Vitro Study ◽  
Blue Dye ◽  
P Value ◽  
Filling Material ◽  
Sealing Ability ◽  
Significant Difference

Introduction: Microleakage is a major setback to restorative dentistry. Leakage at the tooth/restoration interface may lead to numerous problems like postoperative sensitivity, recurrent caries, marginal sealing and later total failure of restoration. Aim: To determine the sealing ability (microleakage) of a new hydraulic calcium silicate cement (Biodentine) as a base material and a bulk filling material. Materials and Methods: An in-vitro study was conducted with ten freshly extracted maxillary molars from March 2019 to June 2019. Standardised Class II cavities were prepared on mesial and distal proximal surfaces. The twenty prepared samples were randomly divided into two groups (n=10)- G1 (BiodentineTM group) and G2 (BiodentineTM + Tetric N-Ceram group). The samples were subjected to thermocycling, followed by 1% methylene blue dye immersion. They were subsequently sectioned both buccolingually and mesiodistally in order to separate to proximal restorations (mesial and distal) and appreciate the amount of dye penetration at tooth/restoration interface respectively. Microleakage was evaluated by observing the samples under a stereomicroscope (30x). The data obtained was analysed using one-way ANOVA test and compared using Post-hoc test. p-value <0.05 to be considered significant. Results: Biodentine showed no significant difference between the sealing ability as a bulk filling and a base material. Group G2 (0.35) indicated comparatively less microleakage as compared to Group G1 (0.6) but the difference in microleakages in both groups was insignificant (p-value 0.083467). Conclusion: BiodentineTM exhibits microleakage when used as a bulk filling material and as a dentin substitute in Class II cavities since there is no significant difference in both the groups.

scholarly journals Effect of acidic environment and intracanal medicament on push-out bond strength of biodentine and mineral trioxide aggregate plus: an in vitro study

2019 ◽  
Author(s):  
Priyanka Jain ◽  
Zinnie Nanda ◽  
Rahul Deore ◽  
Amit Gandhi
Keyword(s):  
Bond Strength ◽  
Calcium Hydroxide ◽  
Testing Machine ◽  
Mineral Trioxide Aggregate ◽  
In Vitro Study ◽  
Vitro Study ◽  
Acidic Environment ◽  
Significant Difference ◽  
Push Out

Introduction. This in-vitro study aims to evaluate the effect of acidic environment and intracanal medicament on push out bond strength of Biodentine and Mineral Trioxide Aggregate Plus (MTA Plus) Method. Forty extracted single rooted teeth were sectioned below the cement-enamel junction. The root canals were instrumented using rotary files and then peeso reamer was used to obtain standardized root canal dimension. Specimens were randomly classified into following groups- Group 1: calcium hydroxide in the absence of acidic environment; Group 2: calcium hydroxide in the presence of acidic environment; Group 3: no intracanal medicament in the absence of acidic environment; Group 4: no intracanal medicament in the presence of acidic environment. Specimens were kept for 7 days at room temperature. Thereafter, specimens of each group were transversely sectioned into 1 mm thick slices and divided into 2 sub-groups according to the use of biodentine and MTA Plus. Using Universal Testing Machine, push out bond strength test was carried out and the data were analyzed statistically. Results. There was no statistically significant difference  in the bond strength of biodentine and MTA Plus (P>0.05). For both MTA Plus and biodentine, with or without calcium hydroxide, the push out bond strength was less in acidic environment and this difference was more pronounced without calcium hydroxide. In all the four groups, MTA plus showed comparable bond strength to biodentine. Conclusion. MTA Plus is a viable option for apexification. The push out bond strength of Biodentine and MTA Plus is impaired by acidic environment. Prior application of calcium hydroxide slightly increased the bond strength, though the difference was statistically  insignificant.    

scholarly journals Prevention of dentine caries using silver diamine fluoride application followed by Er:YAG laser irradiation: an in vitro study

2013 ◽  
Vol 29 (6) ◽  
pp. 1785-1791 ◽  
Author(s):  
May L. Mei ◽  
Leticia Ito ◽  
C. H. Chu ◽  
Edward C. M. Lo ◽  
C. F. Zhang
Keyword(s):  
Laser Irradiation ◽  
Er:Yag Laser ◽  
In Vitro Study ◽  
Vitro Study ◽  
Silver Diamine Fluoride

Synergistic Effect of Er:YAG Laser Irradiation in Combination with Chlorhexidine on the Viability of Enterococcus faecalis: An In Vitro Study

2011 ◽  
Vol 29 (11) ◽  
pp. 753-758 ◽  
Author(s):  
Sharonit Sahar-Helft ◽  
Iris Slutzky-Goldberg ◽  
Joshua Moshonov ◽  
Adam Stabholtz ◽  
Marcos Jacobovitz ◽  
...  
Keyword(s):  
Synergistic Effect ◽  
Laser Irradiation ◽  
Enterococcus Faecalis ◽  
Er:Yag Laser ◽  
In Vitro Study ◽  
Vitro Study
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