scholarly journals Biological Fingerprinting of Herbal Samples by Means of Liquid Chromatography

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Łukasz Cieśla
Keyword(s):  
Quality Control ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
Published Data ◽  
Fingerprint Analysis ◽  
Chromatographic Techniques ◽  
Liquid Chromatographic ◽  
Further Development ◽  
Layer Chromatography ◽  
Chromatographic Fingerprinting

Biological chromatographic fingerprinting is a relatively new concept in the quality control of herbal samples. Originally it has been developed with the application of HPLC, and recently herbal samples' biological profiles have been obtained by means of thin-layer chromatography (TLC). This paper summarizes the application of liquid chromatographic techniques for the purpose of biological fingerprint analysis (BFA) of complex herbal samples. In case of biological TLC fingerprint, which is a relatively novel solution, perspectives of its further development are outlined in more detail. Apart from already published data, some novel results are also shown and briefly discussed. The paper aims at drawing scientists' attention to the unique solutions offered by biological fingerprint construction.

Evaluation of Thin Layer, Paper, and High Performance Liquid Chromatography for Identification of Dyes Extracted as Ion-Pairs with Tri-n-octylamine

1982 ◽  
Vol 65 (3) ◽  
pp. 730-736
Author(s):  
Marc L Puttemans ◽  
Louis Dryon ◽  
Désiré L Massart
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
High Performance ◽  
Ion Pairs ◽  
Chromatographic Behavior ◽  
Reverse Phase ◽  
Food Dyes ◽  
Liquid Chromatographic ◽  
Layer Chromatography

Abstract Food dyes, extracted into chloroform as ion-pairs with tri-n-octylamine, are chromatographed directly in various liquid chromatographic systems. Dyes can be identified by comparison of the chromatographic behavior of ion-pairs with behavior of reference dyes dissolved in water. In thin layer and paper chromatography, the ion-pairs dissociate when polar eluants are used; thin layer chromatography is preferred because of less tailing. In high performance liquid chromatography, a reverse phase ion-pair system is used with tetrabutylammonium as the counter ion; chloroform-extracted dyes show retention times similar to reference dyes dissolved in water.

Determination of morphine by electron capture gas-liquid chromatography.

1977 ◽  
Vol 23 (9) ◽  
pp. 1640-1643 ◽  
Author(s):  
G Nicolau ◽  
G Van Lear ◽  
B Kaul ◽  
B Davidow
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Electron Capture ◽  
Large Scale ◽  
Screening Tests ◽  
Electron Capture Detection ◽  
Gas Liquid Chromatography ◽  
Liquid Chromatographic ◽  
Layer Chromatography

Abstract We describe a micromethod for determining as little as 0.1 microgram of morphine per liter of human urine. The procedure is about 20-fold more sensitive than are currently used gas-liquid chromatographic methods. It is particularly suitable for use as a confirmatory method for large-scale radioimmunoassay screening tests. The procedure involves extraction of morphine from urine and analysis of its heptafluorobutyryl derivative by gas-liquid chromatography with electron capture detection. Morphine can be determined by this procedure over a range of 0.1-200 microgram/liter of urine, 2 pg of the pure derivative being the lowest amount detectable. Urine samples from patients receiving methadone were analyzed by thin-layer chromatography, radioimmunoassay, and our procedure. The results by our procedure agreed well with those obtained by radioimmunoassay.

Phytochemical, Analytical and Medicinal Studies of Holoptelea integrifolia Roxb. Planch - A Review

2019 ◽  
Vol 5 (4) ◽  
pp. 270-277 ◽  
Author(s):  
Vijay Kumar ◽  
Simranjeet Singh ◽  
Ragini Bhadouria ◽  
Ravindra Singh ◽  
Om Prakash
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Biologically Active ◽  
Toxicological Studies ◽  
Wide Range ◽  
Layer Chromatography

Holoptelea integrifolia Roxb. Planch (HI) has been used to treat various ailments including obesity, osteoarthritis, arthritis, inflammation, anemia, diabetes etc. To review the major phytochemicals and medicinal properties of HI, exhaustive bibliographic research was designed by means of various scientific search engines and databases. Only 12 phytochemicals have been reported including biologically active compounds like betulin, betulinic acid, epifriedlin, octacosanol, Friedlin, Holoptelin-A and Holoptelin-B. Analytical methods including the Thin Layer Chromatography (TLC), High-Performance Thin Layer Chromatography (HPTLC), High-Performance Liquid Chromatography (HPLC) and Liquid Chromatography With Mass Spectral (LC-MS) analysis have been used to analyze the HI. From medicinal potency point of view, these phytochemicals have a wide range of pharmacological activities such as antioxidant, antibacterial, anti-inflammatory, and anti-tumor. In the current review, it has been noticed that the mechanism of action of HI with biomolecules has not been fully explored. Pharmacology and toxicological studies are very few. This seems a huge literature gap to be fulfilled through the detailed in-vivo and in-vitro studies.

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