scholarly journals Flow-Based Systems for Rapid and High-Precision Enzyme Kinetics Studies

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Supaporn Kradtap Hartwell ◽  
Kate Grudpan
Keyword(s):  
Enzyme Kinetics ◽  
High Precision ◽  
Initial Rate ◽  
Enzymatic Reaction ◽  
High Volume ◽  
Manual Operation ◽  
Kinetics Studies ◽  
Time Control ◽  
The Many ◽  
Potential Use

Enzyme kinetics studies normally focus on the initial rate of enzymatic reaction. However, the manual operation of steps of the conventional enzyme kinetics method has some drawbacks. Errors can result from the imprecise time control and time necessary for manual changing the reaction cuvettes into and out of the detector. By using the automatic flow-based analytical systems, enzyme kinetics studies can be carried out at real-time initial rate avoiding the potential errors inherent in manual operation. Flow-based systems have been developed to provide rapid, low-volume, and high-precision analyses that effectively replace the many tedious and high volume requirements of conventional wet chemistry analyses. This article presents various arrangements of flow-based techniques and their potential use in future enzyme kinetics applications.

Ultra-high-precision time control system over any long time delay for laser pump and synchrotron x-ray probe experiment

2008 ◽  
Vol 79 (4) ◽  
pp. 045107 ◽  
Author(s):  
Yoshimitsu Fukuyama ◽  
Nobuhiro Yasuda ◽  
Jungeun Kim ◽  
Haruno Murayama ◽  
Takashi Ohshima ◽  
...  
Keyword(s):  
Control System ◽  
Time Delay ◽  
High Precision ◽  
X Ray ◽  
Laser Pump ◽  
Time Control ◽  
Probe Experiment ◽  
Long Time ◽  
Precision Time

scholarly journals Process influences and correction possibilities for high precision injection molded freeform optics

2016 ◽  
Vol 5 (4) ◽  
Author(s):  
Lars Dick ◽  
Stefan Risse ◽  
Andreas Tünnermann
Keyword(s):  
Injection Molding ◽  
High Precision ◽  
Influencing Factor ◽  
High Volume ◽  
Injection Molding Process ◽  
Molding Process ◽  
Shape Accuracy ◽  
Polymer Optics ◽  
Freeform Optics ◽  
Form Deviation

AbstractModern injection molding processes offer a cost-efficient method for manufacturing high precision plastic optics for high volume applications. Besides form deviation of molded freeform optics, internal material stress is a relevant influencing factor for the functionality of a freeform optics in an optical system. This paper illustrates dominant influence parameters of an injection molding process relating to form deviation and internal material stress based on a freeform demonstrator geometry. Furthermore, a deterministic and efficient way for 3D mold correcting of systematic, asymmetrical shrinkage errors is shown to reach micrometer range shape accuracy at diameters up to 40 mm. In a second case, a stress-optimized parameter combination using unusual molding conditions was 3D corrected to reach high precision and low stress freeform polymer optics.

scholarly journals Magnetically driven piezoelectric soft microswimmers for neuron-like cell delivery and neuronal differentiation

2019 ◽  
Vol 6 (7) ◽  
pp. 1512-1516 ◽  
Author(s):  
Xiang-Zhong Chen ◽  
Jia-Hao Liu ◽  
Mei Dong ◽  
Lucas Müller ◽  
George Chatzipirpiridis ◽  
...  
Keyword(s):  
Minimally Invasive ◽  
Neuronal Differentiation ◽  
High Precision ◽  
Cell Delivery ◽  
Biomedical Devices ◽  
Potential Use

Wireless piezoelectric microrobots are biomedical devices with a potential use in high-precision minimally invasive therapies.

scholarly journals Evaluating neuronal and glial growth on electrospun polarized matrices: bridging the gap in percussive spinal cord injuries

2007 ◽  
Vol 3 (2) ◽  
pp. 119-126 ◽  
Author(s):  
Woon N. Chow ◽  
David G. Simpson ◽  
John W. Bigbee ◽  
Raymond J. Colello
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injuries ◽  
Growth Dynamics ◽  
Axonal Guidance ◽  
Directional Growth ◽  
Fiber Alignment ◽  
The Matrix ◽  
The Many ◽  
Spinal Cord Repair ◽  
Potential Use

AbstractOne of the many obstacles to spinal cord repair following trauma is the formation of a cyst that impedes axonal regeneration. Accordingly, we examined the potential use of electrospinning to engineer an implantable polarized matrix for axonal guidance. Polydioxanone, a resorbable material, was electrospun to fabricate matrices possessing either aligned or randomly oriented fibers. To assess the extent to which fiber alignment influences directional neuritic outgrowth, rat dorsal root ganglia (DRGs) were cultured on these matrices for 10 days. Using confocal microscopy, neurites displayed a directional growth that mimicked the fiber alignment of the underlying matrix. Because these matrices are generated from a material that degrades with time, we next determined whether a glial substrate might provide a more stable interface between the resorbable matrix and the outgrowing axons. Astrocytes seeded onto either aligned or random matrices displayed a directional growth pattern similar to that of the underlying matrix. Moreover, these glia-seeded matrices, once co-cultured with DRGs, conferred the matrix alignment to and enhanced outgrowth exuberance of the extending neurites. These experiments demonstrate the potential for electrospinning to generate an aligned matrix that influences both the directionality and growth dynamics of DRG neurites.

Initial Rate Enzyme Kinetics

1977 ◽  
Vol 5 (1) ◽  
pp. 332-332
Author(s):  
PAUL C. ENGEL
Keyword(s):  
Enzyme Kinetics ◽  
Initial Rate

The Krogh revolution: advances in the measurement of timeThis article is the Introduction to a series of papers published in this Special Issue on the theme of Geochronology in honour of Tom Krogh.

2011 ◽  
Vol 48 (2) ◽  
pp. 87-94 ◽  
Author(s):  
Sandra L. Kamo ◽  
Fernando Corfu ◽  
Larry M. Heaman ◽  
Desmond E. Moser
Keyword(s):  
High Precision ◽  
Global Distribution ◽  
Special Issue ◽  
Dating Methods ◽  
The World ◽  
Technical Advances ◽  
Investigative Approach ◽  
The Many ◽  
Geologic Processes

Tom Krogh revolutionized the field of precise U–Pb geochronology through a series of ground-breaking technical advances in the 1970s and 1980s that changed our investigative approach to understanding geologic processes. Earth scientists around the world have used his dating methods for more than 30 years to produce high-precision ages that have advanced our knowledge of Earth’s evolution through time. Tom applied these techniques to investigate the formation of ancient crust in the Superior and Grenville provinces, and other orogens, and the timing of terrestrial impacts. His legacy is built upon these scientific contributions, the many people he trained and inspired, and the global distribution of laboratories that use his methods.

scholarly journals The use of Hydroxychloroquine in the Treatment of COVID-19

2020 ◽  
Vol 25 (3) ◽  
pp. 283-291
Author(s):  
Kamer Tecen-Yücel ◽  
Emre Kara ◽  
Kutay Demirkan ◽  
Serhat Ünal
Keyword(s):  
Infectious Disease ◽  
Treatment Strategies ◽  
World Health Organisation ◽  
World Health ◽  
Pharmacological Properties ◽  
Potential Treatment ◽  
The World ◽  
The Many ◽  
Potential Use ◽  
Human Infectious Disease

COVID-19 is a newly emerging human infectious disease of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) origin described as a pandemic by the World Health Organisation (WHO) on March 11, 2020. There is currently no definitive cure for COVID-19; however, among the many treatment strategies, hydroxychloroquine has been suggested as a potential treatment. The purpose of this article was to review the pharmacological properties and mechanism of COVID-19 treatment with hydroxychloroquine and its potential use on the current COVID-19 pandemic.

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