scholarly journals Changes in the Th1 : Th2 Cytokine Bias in Pregnancy and the Effects of the Anti-Inflammatory Cyclopentenone Prostaglandin 15-Deoxy-Δ12,14-ProstaglandinJ2

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Lynne Sykes ◽  
David A. MacIntyre ◽  
Xiao J. Yap ◽  
Sathana Ponnampalam ◽  
Tiong Ghee Teoh ◽  
...  
Keyword(s):  
Proinflammatory Cytokines ◽  
T Helper Cells ◽  
Interleukin 4 ◽  
Th2 Cells ◽  
T Helper ◽  
Th2 Response ◽  
Helper Cells ◽  
Th2 Cytokine ◽  
Anti Inflammatory ◽  
In Pregnancy

Pregnancy is a complex immunological state in which a bias towards T helper 2 (Th2) protects the fetus. Evidence suggests that proinflammatory cytokines increase the risk of poor neonatal outcome, independently of the direct effect of preterm labour. The anti-inflammatory prostaglandin 15-deoxy-Δ12,14-Prostaglandin J2(15dPGJ2) inhibits nuclear factor Kappa B (NF-κB) in amniocytes and myocytesin vitroand is a ligand for the chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) receptor. Here we examine the Th1:Th2 cytokine bias in pregnancy and whether 15dPGJ2could be used to inhibit the production of the proinflammatory cytokines through inhibition of NF-κB while simultaneously promoting Th2 interleukin 4 (IL-4) synthesis via CRTH2 in T helper cells. Peripheral blood mononuclear cells (PBMCs) from women at 28 weeks, term pre-labour, term labour as well as non-pregnant female controls were cultured with 15dPGJ2or vehicle control and stimulated with phorbol myristyl acetate (PMA)/ionomycin. The percentage of CD4+cells producing interferon gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α) in response to PMA/ionomycin was significantly reduced in pregnancy. 15dPGJ2reduced IFN-γand TNF-αproduction in stimulated T helper cells, but did not alter IL-4 production in CRTH2+vecells. 15dPGJ2also reduced phospho-p65 in stimulated PBMCs. In summary, 15dPGJ2suppresses the Th1 response of PBMCs during pregnancy and active labour whilst maintaining the Th2 response suggesting a therapeutic benefit in reducing neonatal morbidity in inflammation-induced PTL.

Peripheral Blood T-Helper Cells and Eosinophil Populations in Patients with Atopic and Nonatopic Chronic Rhinosinusitis

2017 ◽  
Vol 31 (1) ◽  
pp. e8-e12 ◽  
Author(s):  
Zhenxiao Huang ◽  
Jayakar V. Nayak ◽  
Yan Sun ◽  
Qian Huang ◽  
Bing Zhou
Keyword(s):  
Chronic Rhinosinusitis ◽  
Peripheral Blood ◽  
T Helper Cells ◽  
Nasal Polyps ◽  
Interferon Γ ◽  
Interleukin 4 ◽  
Th2 Cells ◽  
T Helper ◽  
Helper Cells ◽  
Th1 And Th2

Background Analysis of recent research indicated that T-helper cells may play an important role in the pathogenesis of chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) and CRS without nasal polyps (CRSsNP). Objective The purpose of this study was to investigate the peripheral blood Th1 and Th2 cells and eosinophil population in patients with CRS. Methods Peripheral blood samples were obtained from nine nonatopic controls, 37 patients with CRSsNP, and 66 patients with CRSwNP. The samples were then analyzed by flow cytometry analysis (Th1 cell [CD4+, interleukin 4−, interferon γ+]; and Th2 cell [CD4+, interleukin 4+, interferon γ−]). The patients were stratified into four groups based on their allergic status by using skin-prick test results and immunoglobulin E level measurements as the following: (1) nonatopic CRSsNP, (2) nonatopic CRSwNP, (3) atopic CRSsNP, and (4) atopic CRSwNP. Eosinophil counts were also compared. The severity of nasal diseases in these patients was assessed via the Lund-Mackay score. Results No significant differences in peripheral blood Th1 and Th2 cells were found among all the atopic, nonatopic CRS groups, and the nonatopic control groups. Peripheral blood eosinophil levels in atopic CRSwNP were significantly elevated compared with the nonatopic controls (p < 0.05), but no significant difference was found among all atopic and nonatopic CRS groups. Conclusion Analysis of our data demonstrated that a proportion of systemic Th1- and Th2-skewed lymphocytes in all CRS groups were similar to that in healthy subjects, irrespective of atopic status. The patients with CRSwNP and with atopy but not the patients with CRSsNP and with atopy demonstrated systemic eosinophilic inflammation. Further studies are needed to investigate underlying pathophysiologic mechanism or endotypes.

T helper cells grown with hapten-modified cultured Langerhans' cells produce interleukin 4 and stimulate IgE production by B cells

1989 ◽  
Vol 19 (2) ◽  
pp. 245-252 ◽  
Author(s):  
Conrad Hauser ◽  
Clifford M. Snapper ◽  
Junichi Ohara ◽  
William E. Paul ◽  
Stephen I. Katz
Keyword(s):  
B Cells ◽  
T Helper Cells ◽  
Langerhans Cells ◽  
Interleukin 4 ◽  
T Helper ◽  
Helper Cells

Fluorescent-activated cell-sorting analysis of intracellular interferon-γ and interleukin-4 in fresh and frozen human peripheral blood T-helper cells

2005 ◽  
Vol 13 (4) ◽  
pp. 441-449 ◽  
Author(s):  
Ruhangiz T. Kilani ◽  
Megan Delehanty ◽  
Heather A. Shankowsky ◽  
Aziz Ghahary ◽  
Paul Scott ◽  
...  
Keyword(s):  
Cell Sorting ◽  
Peripheral Blood ◽  
T Helper Cells ◽  
Human Peripheral Blood ◽  
Interferon Γ ◽  
Interleukin 4 ◽  
T Helper ◽  
Helper Cells

Up-regulated interleukin-4 production by peripheral T-helper cells in idiopathic membranous nephropathy

2004 ◽  
Vol 19 (3) ◽  
pp. 580-586 ◽  
Author(s):  
K. Masutani ◽  
M. Taniguchi ◽  
H. Nakashima ◽  
H. Yotsueda ◽  
Y. Kudoh ◽  
...  
Keyword(s):  
Membranous Nephropathy ◽  
T Helper Cells ◽  
Interleukin 4 ◽  
Idiopathic Membranous Nephropathy ◽  
T Helper ◽  
Helper Cells

scholarly journals Early Growth Response Protein-1 (Egr-1) Is Preferentially Expressed in T Helper Type 2 (Th2) Cells and Is Involved in Acute Transcription of the Th2 Cytokine Interleukin-4

2009 ◽  
Vol 285 (3) ◽  
pp. 1643-1652 ◽  
Author(s):  
Michael Lohoff ◽  
Marco Giaisi ◽  
Rebecca Köhler ◽  
Bärbel Casper ◽  
Peter H. Krammer ◽  
...  
Keyword(s):  
Growth Response ◽  
Early Growth ◽  
Interleukin 4 ◽  
Th2 Cells ◽  
T Helper ◽  
Early Growth Response ◽  
Th2 Cytokine ◽  
Helper Type
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