scholarly journals Optimal Timing for Oocyte Denudation and Intracytoplasmic Sperm Injection

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Catherine Patrat ◽  
Aida Kaffel ◽  
Lucie Delaroche ◽  
Juliette Guibert ◽  
Pierre Jouannet ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Oocyte Retrieval ◽  
Optimal Combination ◽  
Optimal Timing ◽  
Male Factor Infertility ◽  
Male Factor ◽  
Female Age ◽  
Fertilisation Rate ◽  
The Impact ◽  
Long Protocol

Objectives. To analyze the impact of oocyte denudation and microinjection timings on intracytoplasmic sperm injection (ICSI) outcomes.Study Design. We included ICSI cycles with the following parameters: rank 1 or 2, female age years, male factor infertility, long protocol using GnRH agonist and rFSH for ovarian stimulation, and use of freshly ejaculated sperm (). Several ICSI parameters were analyzed according to the time between oocyte retrieval and denudation () and the time between denudation and ICSI () using a statistical logistic regression analysis.Results. Neither nor had a significant influence on the Metaphase II (MII) rate but the fertilisation rate (FR) showed a significant improvement when was longer (optimal results at hours) while FR significantly decreased with the increase of . Optimal implantation (IR) and pregnancy (PR) rates were obtained when was around 2 hours.Conclusion. Incubation of oocytes around 2 hours between retrieval and denudation may not increase MII rate but appears to lead to the optimal combination of FR and IR.

scholarly journals In vitro fertilisation (IVF) versus intracytoplasmic sperm injection (ICSI) in patients without severe male factor infertility: study protocol for the randomised, controlled, multicentre trial INVICSI

BMJ Open ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. e051058
Author(s):  
Sine Berntsen ◽  
Bugge Nøhr ◽  
Marie Louise Grøndahl ◽  
Morten Rønn Petersen ◽  
Lars Franch Andersen ◽  
...  
Keyword(s):  
Live Birth ◽  
Intracytoplasmic Sperm Injection ◽  
Controlled Trial ◽  
Male Partner ◽  
In Vitro Fertilisation ◽  
Male Factor Infertility ◽  
Male Factor ◽  
Fertilisation Rate ◽  
Randomised Controlled

IntroductionOver the last decades, the use of intracytoplasmic sperm injection (ICSI) has increased, even among patients without male factor infertility. The increase has happened even though there is no evidence to support that ICSI results in higher live birth rates compared with conventional in vitro fertilisation (IVF) in cases with nonmale factor infertility. The lack of robust evidence on an advantage of using ICSI over conventional IVF in these patients is problematic since ICSI is more invasive, complex and requires additional resources, time and effort. Therefore, the primary objective of the IVF versus ICSI (INVICSI) study is to determine whether ICSI is superior to standard IVF in patients without severe male factor infertility. The primary outcome measure is first live birth from fresh and frozen-thawed transfers after one stimulated cycle. Secondary outcomes include fertilisation rate, ongoing pregnancy rate, birth weight and congenital anomalies.Methods and analysisThis is a two-armed, multicentre, randomised, controlled trial. In total, 824 couples/women with infertility without severe male factor will be recruited and allocated randomly into two groups (IVF or ICSI) in a 1:1 ratio. Participants will be randomised in variable block sizes and stratified by trial site and age. The main inclusion criteria are (1) no prior IVF/ICSI treatment, (2) male partner sperm with an expected count of minimum 2 million progressive motile spermatozoa following density gradient purification on the day of oocyte pick up and (3) age of the woman between 18 and 42 years.Ethics and disseminationThe study will be performed in accordance with the ethical principles in the Helsinki Declaration. The study is approved by the Scientific Ethical Committee of the Capital Region of Denmark. Study findings will be presented, irrespectively of results at international conferences and submitted for publication in peer-reviewed journals.Trial registration numberNCT04128904. Pre-results.

scholarly journals Intracytoplasmic sperm injection: state of the art in humans

Reproduction ◽  
2017 ◽  
Vol 154 (6) ◽  
pp. F93-F110 ◽  
Author(s):  
G D Palermo ◽  
C L O’Neill ◽  
S Chow ◽  
S Cheung ◽  
A Parrella ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Poor Prognosis ◽  
State Of The Art ◽  
Semen Analysis ◽  
Reproductive Technology ◽  
Male Factor Infertility ◽  
Male Factor ◽  
Current Application ◽  
Infertile Couples ◽  
The Impact

Among infertile couples, 25% involve both male and female factors, while male factor alone accounts for another 25% due to oligo-, astheno-, teratozoospermia, a combination of the three, or even a complete absence of sperm cells in the ejaculate and can lead to a poor prognosis even with the help of assisted reproductive technology (ART). Intracytoplasmic sperm injection (ICSI) has been with us now for a quarter of a century and in spite of the controversy generated since its inception, it remains in the forefront of the techniques utilized in ART. The development of ICSI in 1992 has drastically decreased the impact of male factor, resulting in millions of pregnancies worldwide for couples who, without ICSI, would have had little chance of having their own biological child. This review focuses on the state of the art of ICSI regarding utility of bioassays that evaluate male factor infertility beyond the standard semen analysis and describes the current application and advances in regard to ICSI, particularly the genetic and epigenetic characteristics of spermatozoa and their impact on reproductive outcome.

scholarly journals The Impact of Intracytoplasmic Sperm Injection in Non-Male Factor Infertility—A Critical Review

2021 ◽  
Vol 10 (12) ◽  
pp. 2616
Author(s):  
Tanya L. Glenn ◽  
Alex M. Kotlyar ◽  
David B. Seifer
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Live Birth Rate ◽  
Fertilization Rate ◽  
Male Factor Infertility ◽  
Fertilization Failure ◽  
Male Factor ◽  
Vitro Fertilization ◽  
Total Fertilization Failure ◽  
The Impact

Intracytoplasmic sperm injection (ICSI) was originally designed to overcome barriers due to male factor infertility. However, a surveillance study found that ICSI use in non-male factor infertility increased from 15.4% to 66.9% between 1996 and 2012. Numerous studies have investigated fertilization rate, total fertilization failure, and live birth rate per cycle (LBR), comparing the use of ICSI versus conventional in vitro fertilization (IVF) for non-male factor infertility. The overwhelming conclusion shows no increase in fertilization rate or LBR per cycle with the use of ICSI for non-male factor infertility. The overuse of ICSI is likely related to the desire to avoid a higher rate of total fertilization failure in IVF. However, data supporting the benefit of using ICSI for non-male factor infertility is lacking, and 33 couples would need to be treated with ICSI unnecessarily to avoid one case of total fertilization failure. Such practice increases the cost to the patient, increases the burden on embryologist’s time, and is a misapplication of resources. Additionally, there remains conflicting data regarding the safety of offspring conceived by ICSI and potential damage to the oocyte. Thus, the use of ICSI should be limited to those with male factor infertility or a history of total fertilization factor infertility due to uncertainties of potential adverse impact and lack of proven benefit in non-male factor infertility.

P-244 Hysteroscopic tubal transfer of ova after intracytoplasmic sperm injection (ICSI) as a treatment for severe male factor infertility

1997 ◽  
Vol 68 ◽  
pp. S209
Author(s):  
J.J Knickerbocker ◽  
K.A Thompson ◽  
E.A Riley ◽  
G Lopez ◽  
M Carpentieri ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Male Factor Infertility ◽  
Male Factor
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