scholarly journals Wound Healing and Anti-Inflammatory Effect in Animal Models ofCalendula officinalisL. Growing in Brazil

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Leila Maria Leal Parente ◽  
Ruy de Souza Lino Júnior ◽  
Leonice Manrique Faustino Tresvenzol ◽  
Marina Clare Vinaud ◽  
José Realino de Paula ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Chorioallantoic Membrane ◽  
Ethanolic Extract ◽  
Immunohistochemical Analysis ◽  
Antibacterial Activities ◽  
Cutaneous Wounds ◽  
Annual Herb ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Calendula officinalisis an annual herb from Mediterranean origin which is popularly used in wound healing and as an anti-inflammatory agent. In this study, the ethanolic extract, the dichloromethane, and hexanic fractions of the flowers from plants growing in Brazil were produced. The angiogenic activity of the extract and fractions was evaluated through the chorioallantoic membrane and cutaneous wounds in rat models. The healing activity of the extract was evaluated by the same cutaneous wounds model through macroscopic, morphometric, histopathologic, and immunohistochemical analysis. The antibacterial activity of the extract and fractions was also evaluated. This experimental study revealed thatC. officinalispresented anti-inflammatory and antibacterial activities as well as angiogenic and fibroplastic properties acting in a positive way on the inflammatory and proliferative phases of the healing process.

scholarly journals Ekspresi COX-2 dan Jumlah Neutrofil Fase Inflamasi pada Proses Penyembuhan Luka Setelah Pemberian Sistemik Ekstrak Etanolik Rosela (Hibiscus sabdariffa) (studi in vivo pada Tikus Wistar)

2014 ◽  
Vol 21 (1) ◽  
pp. 13
Author(s):  
Endah Kusumastuti ◽  
Juni Handajani ◽  
Heni Susilowati
Keyword(s):  
Wound Healing ◽  
Tissue Injury ◽  
Healing Process ◽  
Ethanolic Extract ◽  
Systemic Administration ◽  
Control Group ◽  
Hibiscus Sabdariffa ◽  
Inflammatory Phase ◽  
Cox 2 ◽  
Anti Inflammatory

Inflamasi merupakan respon alami tubuh terhadap adanya kerusakan jaringan. Salah satu medikamen untuk mengatasi inflamasi adalah antiinflamasi non steroid (AINS). Penggunaan AINS mempunyai beberapa efek samping dan dalam beberapa hal penggunaan tanaman obat dinilai lebih aman. Rosela merupakan salah satu tanaman obat yang mempunyai potensi sebagai antiinflamasi. Penelitian ini bertujuan untuk mengetahui efek pemberian sistemik ekstrak etanolik rosela terhadap ekspresi COX-2 dan jumlah neutrofil fase inflamasi pada proses penyembuhan luka. Bunga rosela didapatkan dari perkebunan di Dusun Bulusari Desa Pojok Kecamatan Tarokan Kabupaten Kediri Jawa Timur. Pembuatan ekstrak rosela dilakukan di LPPT unit I UGM Yogjakarta dengan cara perkolasi. Tikus putih galur Wistar sebanyak 36 ekor diberi perlukaan dengan punch biopsi ɵ 3 mm pada mukosa bukal. Subjek dibagi menjadi 3 kelompok, masing-masing kelompok 12 ekor tikus. Pembagian kelompok terdiri dari kontrol negatif (saline), kontrol positif (ibuprofen 20 mg/kg BB) dan perlakuan (ekstrak rosela 500 mg/kg BB). Pemberian minum sesuai kelompoknya sehari sekali selama 4 hari. Pada hari ke-1, ke-2, ke-3 dan ke-4 tikus dikorbankan lalu jaringan mukosa yang mengalami perlukaan dibuat preparat histologis. Pewarnaan Hematoksilin Eosin (HE) dilakukan untuk mengamati jumlah neutrofil. Ekspresi COX-2 diamati pada preparat dengan pewarnaan imunohistokimia menggunakan rabbit polyclonal antibody COX-2 (Lab Vision, USA). Jumlah neutrofil dan ekspresi COX-2 dihitung di bawah mikroskop cahaya lalu data dianalisi menggunakan ANAVA dan LSD. Hasil penelitian menunjukkan bahwa ekspresi COX-2 dan jumlah neutrofil lebih rendah pada kelompok perlakuan dibanding kontrol. Pengamatan klinis pada hari ke-4 juga tampak luka seluruh subjek telah menutup sempurna setelah pemberian minum rosela. Disimpulkan bahwa ekstrak etanolik rosela mempunyai kemampuan menghambat ekspresi COX-2 dan menurunkan jumlah neutrofil sehingga dapat digunakan sebagai bahan anti-inflamasi. Expression of COX-2 and The Number of Neutrophil in Inflammation stage of Wound Healing Process after Systemic Administration of Ethanolic Extract Rosela. Inflammation is an initial stage of body’s natural response to tissue damage.The use  empirically plants often used for traditional medicine because it is easily found in the community and fewer side effects. Flavanoid presence of roselle (Hibiscus sabdariffa) is thought to have anti inflammatory effects. This study aimed to know the effect of systemic administration of Roselle ethanolic extract toward COX-2 expression and neutrophils number in the inflammatory phase of wound healing processes. Roselle was obtained from plantations in Bulusari hamlet, Tarokan, Kediri, EastJava. Making roselle extract was performed in LPPT unit 1 UGM Yogyakarta by percolation ways. Wistar rats were given a total of 36 injuries with ɵ 3 mm punch biopsy of the buccal mucosa. Subjects were divided into three groups, each group of 12 rats. The division consists of the negative control group (saline), positive control (ibuprofen 20 mg/kg) and treatment (roselle extract 500 mg/kg). Giving drink suitable group once daily for four days. On day 1, the 2nd, 3rd and fourth rats were sacrificed, and mucosal tissue injury was made histological preparat. Hematoxylin eosin staining (HE) was performed to observe the number of neutrophils. COX-2 expression was found in preparations for immunohistochemical staining using rabbit polyclonal COX-2 antibody (Lab Vision, USA). The number of neutrophils and expression of COX-2 is calculated under a light microscope data were analyzed using Two-way ANOVA and LSD. The results showed that the expression of COX-2 and neutrophil number were least in the treatment group compared to the control. Clinical observation on day four also appears around the wound has completely closed the subject after administration of roselle drink. It was concluded that the ethanolic extract of roselle can inhibit COX-2 expression and decrease the number of neutrophils that can be used as an anti-inflammatory ingredient. 

scholarly journals The Wound-Healing Effect of Mango Peel Extract on Incision Wounds in a Murine Model

Molecules ◽  
2022 ◽  
Vol 27 (1) ◽  
pp. 259
Author(s):  
Lesslie Espinosa-Espinosa ◽  
Leticia Garduño-Siciliano ◽  
Mario Rodriguez-Canales ◽  
Luis Barbo Hernandez-Portilla ◽  
Maria Margarita Canales-Martinez ◽  
...  
Keyword(s):  
Wound Healing ◽  
Histological Analysis ◽  
Mangifera Indica ◽  
Normal Skin ◽  
Healing Process ◽  
Antibacterial Activities ◽  
Wound Contraction ◽  
Wound Healing Process ◽  
Mango Peel ◽  
Anti Inflammatory

Mangifera indica can generate up to 60% of polluting by-products, including peels. However, it has been shown that flavonoids and mangiferin are mainly responsible for the antioxidant, anti-inflammatory, and antibacterial activities closely related to the wound-healing process. The chemical composition of MEMI (methanolic extract of M. indica) was analyzed by HPLC-DAD, as well as concentrations of total phenol (TPC) and flavonoids (TFC) and antioxidant activity (SA50). Wound-healing efficacy was determined by measurements of wound contraction, histological analysis, and tensiometric method; moreover, anti-inflammatory, antibacterial, and acute dermal toxicity (OECD 402) were also evaluated. Phenol, resorcinol, conjugated resorcinol, and mangiferin were detected. TPC, TFC, and SA50 were 136 mg GAE/g, 101.66 mg QE/g, and 36.33 µg/mL, respectively. Tensile strength and wound contraction closure did not show significant differences between MEMI and dexpanthenol groups. Histological analysis (after 14 days) shows a similar architecture between MEMI treatment and normal skin. MEMI exhibits a reduction in edema. Staphylococcus epidermidis had an MIC of 2 mg/mL, while Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli reached 4 mg/mL. The MEMI showed no signs of toxicity. Therefore, this study demonstrates multiple targets that flavonoids and mangiferin of MEMI may present during the healing process.

scholarly journals Anti Inflammatory Effect Of Toman Fish (Channa micropeltes) Extract In Wound Healing Process

2018 ◽  
Vol 14 (2) ◽  
pp. 83
Author(s):  
Maharani Laillyza Apriasari ◽  
Dewi Puspitasari ◽  
Amy Nindia Carabelly
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Healing Process ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Wound Healing Process ◽  
Inflammatory Phase ◽  
Anti Inflammatory ◽  
Inflammatory Effect

 Abstract: One phase of wound healing is the inflammatory phase. Haruan fish extract has shown to accelerate the healing process up because it has anti-inflammatory effects. Toman fish originates from the same genus as the Haruan fish, but its anti-inflammatory effect remains unknown. It was done to analyzed anti-inflammatory effects of Toman fish extract. This study was true experimental design with posttest-only control design. Twenty-seven male mice of Balb-C strain were divided into 3 groups. Incision wound of 1 cm was made along the back of the mice. Nine mice in each group were sacrificed on 3rd days, then histopathology examination was conducted with Haematoxylin eosin. There were significant differences between treatment group given Toman fish extract and positive control group given Haruan fish extract, and also compared to the negative control group in PMN cell examination. There were significant differences between treatment group given Toman fish extract and positive control group given Haruan fish extract compared to negative controls on the number of macrophage cells. Toman fish extract could lower the number of PMN cells and was able to increase the number of macrophages on the 3rd days. Toman fish has anti-inflammatory effects on the wound healing process. Keywords: Anti-inflammatory effects, haruan fish extract, macrophages, PMN cells, toman fish extract

scholarly journals Anti-Inflammatory Activity of Extract Mixture of Annona senegalensis Pers. and Piliostigma thonningii (Schum.)

2020 ◽  
Vol 10 (6-s) ◽  
pp. 103-108
Author(s):  
Komlatsè Togbenou ◽  
Kokou Idoh ◽  
Kossivi Dosseh ◽  
Tchazou Kpatcha ◽  
Amégnona Agbonon
Keyword(s):  
Nitric Oxide ◽  
Ethanolic Extract ◽  
No Production ◽  
Leukocyte Infiltration ◽  
Inflammation Markers ◽  
Asthma Model ◽  
Piliostigma Thonningii ◽  
Anti Inflammatory ◽  
Inflammatory Component ◽  
Inflammatory Effect

Introduction: Annona senegalensis Pers (Annonaceae) and Piliostigma thonningii (Schum.) (Leguminoseae)  are two medicinal plants used, often in combination, in traditional Togolese medicine for the treatment of diseases with an inflammatory component. Objective: The aim of this study is to evaluate the anti-inflammatory effect of the hydro-ethanolic extract (EHEM) of the combination of A. senegalensis and P. thonningii (1: 1, m: m). Methods: The ovalbumin-induced allergic airway asthma model was used. Animals made asthmatic were treated with EHEM at doses of 250 and 500 mg / kg. Inflammation markers including histamine, nitric oxide (NO), vascular leakage, leukocyte infiltration in the airways, and malondialdehyde (MDA), were measured. Results: Compared to the SNT group, EHEM inhibits the infiltration of the airways by leukocytes (850,00 × 103 ± 50 cells / mL vs 1830 × 103 ± 53,85 cells / mL for ST500 mg / kg, P <0,05). It inhibited vascular permeability to Evans Blue (10,40 ± 0,270 μg / mL vs ST500, p<0,05). It inhibited histamine release (13,95 ± 0,937 μg / mL vs 32,78 ± 1,044 μg / mL, p<0,05) and NO production (0,211 ± 0,008 Μm vs 0,315 ± 0,022 μM, p<0,05). It finally inhibited MDA production (14,66 ± 0,533 nM / mL vs 9,014 ± 0,366 nM / mL and 7,149 ± 0,300 nM / mL, p<0,05) in lung tissue. Conclusion: Our results suggest that EHEM inhibits OVA-induced inflammation. These results justify the use of this combination of plants in traditional Togolese medicine. Keywords: Inflammation, malondialdehyde, Annona senegalensis, Piliostigma thonningii.

Acceleration of the healing process of full-thickness wounds using hydrophilic chitosan–silica hybrid sponge in a porcine model

2018 ◽  
Vol 32 (8) ◽  
pp. 1011-1023 ◽  
Author(s):  
Ji-Ung Park ◽  
Seol-Ha Jeong ◽  
Eun-Ho Song ◽  
Juha Song ◽  
Hyoun-Ee Kim ◽  
...  
Keyword(s):  
Wound Healing ◽  
Surface Characterization ◽  
Porcine Model ◽  
Healing Process ◽  
Synergetic Effect ◽  
Wound Healing Process ◽  
Cutaneous Wounds ◽  
Chemical Coupling

In this study, we evaluated the surface characterization of a novel chitosan–silica hybridized membrane and highlighted the substantial role of silica in the wound environment. The chemical coupling of chitosan and silica resulted in a more condensed network compared with pure chitosan, which was eventually able to stably maintain its framework, particularly in the wet state. In addition, we closely observed the wound-healing process along with the surface interaction between chitosan–silica and the wound site using large-surface-area wounds in a porcine model. Our evidence indicates that chitosan–silica exerts a synergetic effect of both materials to promote a remarkable wound-healing process. In particular, the silica in chitosan–silica accelerated wound closure including wound contraction, and re-epithelialization via enhancement of cell recruitment, epidermal maturity, neovascularization, and granulation tissue formation compared with pure chitosan and other commercial dressing materials. This advanced wound dressing material may lead to effective treatment for problematic cutaneous wounds and can be further applied for human skin regeneration.

scholarly journals Efficacy of application of Equisetum pyramidale Goldm. hydrogel for tissue restoration of induced skin lesions in Wistar rats

2020 ◽  
Vol 80 (1) ◽  
pp. 12-22 ◽  
Author(s):  
F. Carmignan ◽  
R. Matias ◽  
C. A. Carollo ◽  
D. M. Dourado ◽  
M. H. Fermiano ◽  
...  
Keyword(s):  
Wistar Rats ◽  
High Efficiency ◽  
Healing Process ◽  
Ethanolic Extract ◽  
Skin Lesions ◽  
Phytochemical Analysis ◽  
Cutaneous Wounds ◽  
Tissue Restoration ◽  
Layer Chromatography ◽  
Flavonoid Patterns

Abstract Medicinal plants, such as E. pyramidale, are alternatives against infections and assist in the healing process of wounds. We evaluated the effects of Hydrogel of E. pyramidale on the healing of cutaneous wounds in animal models by morphological, morphometric and histological analyses, and elucidated major components. The ethanolic extract of E. pyramidale shoots was submitted to phytochemical analysis. For cicatrizing activity assay, Wistar rats were used, and the groups evaluated were hydrogel containing ethanolic extract of E. pyramidale at 2% compared to control groups. The treatment periods were 3, 7 and 21 days, and macroscopic and microscopic analyses were performed. Data were submitted to Analysis of Variance (p <0.05). Phytochemistry and quantification evidenced that flavonoids are main constituents and that they were evidenced by thin layer chromatography (TLC), high efficiency (HPLC) and infrared (IR) methods. In TLC, using flavonoid patterns, we observed pinocembrin. The hydrogel containing ethanolic extract of E. pyramidale at 2% was effective in wound regression. E. pyramidale can be used for the treatment of second intention wounds, and the effective healing may be due to a high flavonoid content.

scholarly journals Sesquiterpenoids and Their Anti-Inflammatory Activity: Evaluation of Ainsliaea yunnanensis

Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1701
Author(s):  
Jinjie Li ◽  
Xiuting Li ◽  
Xin Wang ◽  
Xiangjian Zhong ◽  
Linlin Ji ◽  
...  
Keyword(s):  
Nlrp3 Inflammasome ◽  
Ethanolic Extract ◽  
The Self ◽  
Inflammatory Activity ◽  
Spectroscopic Methods ◽  
Activity Evaluation ◽  
Caspase 1 ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Four new sesquiterpenoids (1–4) and six known sesquiterpenoids (5–10), were isolated from the EtOAc phase of the ethanolic extract of Ainsliaea yunnanensis. Their structures were established by spectroscopic methods, including 1-D, 2-D NMR and HPLC-MS. All compounds were tested for their anti-inflammatory effect by the inhibition of the activity of NLRP3 inflammasome by blocking the self-slicing of pro-caspase-1, which is induced by nigericin, then the secretion of mature IL-1β, mediated by caspase-1, was suppressed. Unfortunately none of the compounds showed an anti-inflammatory effect.

scholarly journals Anti-Inflammatory Effect of Ethanolic Extract of Carica Papaya Leaves and Indomethacin in Cotton Pellet Induced Granuloma in Animal Model

2019 ◽  
Vol 6 (1) ◽  
pp. 2-5 ◽  
Author(s):  
Afroza Sultana ◽  
Rumana Afroz ◽  
Onayza Yasmeen ◽  
Muqbula Tasrin Aktar ◽  
Md Abdullah Yusuf
Keyword(s):  
Animal Model ◽  
Carica Papaya ◽  
Ethanolic Extract ◽  
Granuloma Formation ◽  
Rat Paw Edema ◽  
Group I ◽  
Cotton Pellet ◽  
Anti Inflammatory ◽  
Group Ii ◽  
Inflammatory Effect

Background: Anti-inflammatory effect can be exerted by Carica papaya. Objective: The purpose of the present study was to see the anti-inflammatory effect of ethanolic extract of Carica papaya leaves and indomethacin in carrageenan induced rat paw edema animal model. Methodology: This was an animal study carried out in the Department of Pharmacology at Dhaka Medical College, Dhaka, Bangladesh during the period from July 2014 to June 2015 for a period of one (01) year. The leaves of Carica Papaya collected from Botanical garden, Mirpur, Dhaka, Bangladesh. The animals were divided into four groups. The animals were divided into four groups. In all the animals granuloma were induced by implantation of autoclaved cotton pellet on the 1st day and they treated as follows: Group I were served as control that was received normal saline. Group II were received ethanolic extract of Carica Papaya leaves. Group III were received ethanolic extract of Carica Papaya leaves. Group IV were received indomethacin. On the 15th day animals were anesthetized, implanted pellets were dissected out, dried at hit air oven and the final weights were measured. Result: The experiment was carried out on 48 Long Evan Norwegian rats. Rats were divided in 4 groups of six animals each. The mean increase of weight of cotton pellet was 48.06±1.02, 24.48±0.36, 22.42±0.56 and 18.02±0.43 in group I, II, III and IV respectively. The inhibition of granuloma formation was 49.06%, 53.35% and 62.51% in group II, III and IV respectively. Conclusion: In conclusion the ethanolic extract of Carica Papaya leaves has effects on cotton pellet induced granuloma formation in experimental rat. Journal of Current and Advance Medical Research 2019;6(1):2-5

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