scholarly journals Characterisation of Flavonoid Aglycones by Negative Ion Chip-Based Nanospray Tandem Mass Spectrometry

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Paul J. Gates ◽  
Norberto P. Lopes
Keyword(s):  
Mass Spectrometry ◽  
Reliable Method ◽  
Biological Activities ◽  
Distinct Pattern ◽  
Negative Ion ◽  
Tandem Mass ◽  
Flavonoid Aglycones ◽  
Product Ions ◽  
Low Mass ◽  
Tea Extract

Flavonoids are one of the most important classes of natural products having a wide variety of biological activities. There is wide interest in a range of medical and dietary applications, and having a rapid, reliable method for structural elucidation is essential. In this study a range of flavonoid standards are investigated by chip-based negative ion nanospray mass spectrometry. It was found that the different classes of flavonoid studied have a combination of distinct neutral losses from the precursor ion [M-H]−along with characteristic low-mass ions. By looking only for this distinct pattern of product ions, it is possible to determine the class of flavonoid directly. This methodology is tested here by the analysis of a green tea extract, where the expected flavonoids were readily identified, along with quercetin, which is shown to be present at only about 2% of the most intense ion in the spectrum.

Fragmentation Studies of Monensin A and B in Negative Electrospray and Nanospray Tandem Mass Spectrometry

2007 ◽  
Vol 13 (3) ◽  
pp. 191-198 ◽  
Author(s):  
José N. Sousa-Junior ◽  
Paul J. Gates ◽  
Norberto P. Lopes
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Major Product ◽  
Negative Ion ◽  
Tandem Mass ◽  
Fragmentation Chemistry ◽  
Product Ions ◽  
Low Mass ◽  
Monensin A ◽  
Negative Ion Mode

This paper reports the first comparative study of the gas-phase fragmentation chemistry of monensin in negative ion mode electrospray and nanoelectrospray tandem mass spectrometry. The fragmentation was observed to occur at lower energies in nanoelectrospray than electrospray. The major product ions are proposed to be formed via an initial neutral elimination of methanol followed be subsequent fragmentation. The low-mass product ions were observed at the same m/z for both monensin A and B.

scholarly journals Simultaneous Determination of Flavonoids from Bamboo Leaf Extracts Using Liquid Chromatography-Tandem Mass Spectrometry

2021 ◽  
Author(s):  
Xiabing Li ◽  
Wuqun Tao ◽  
Hang Xun ◽  
Xi Yao ◽  
Jin Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Simultaneous Determination ◽  
Negative Ion ◽  
Tandem Mass ◽  
Bamboo Species ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

AbstractAn analytical method for the simultaneous determination of ten major functional flavonoids (isoorientin, orientin, vitexin, isovitexin, apigenin, luteolin, tricin, quercetin, rutin, and kaempferol) in different bamboo species was developed by liquid chromatography-tandem mass spectrometry. Chromatographic separation was carried out on a reversed-phase C-18 column with acetonitrile and water as the mobile phases. Detection was performed in negative ion electrospray ionization mode using multiple reaction monitoring mode. The correlation coefficients for the calibration curves ranged from 0.9955 to 0.9997. The limit of detection ranged from 1 to 45 ng/ml. The applicability of this analytical approach was confirmed by the successful analysis of real leaf samples of four bamboo species, family Poaceae: Pleioblastus amarus (Keng) Keng f., Phyllostachys glauca McClure, Phyllostachys edullis (Carrière) J.Houz, and Indocalamus latifolius (Keng) McClure. The total flavonoid contents were 3321.09, 3095.96, 4037.33, and 2808.42 mg/kg for P. amarus, P. glauca, P. edullis, and I. latifolius, respectively. Graphical abstract

scholarly journals Isotopologue distributions of peptide product ions by tandem mass spectrometry: Quantitation of low levels of deuterium incorporation

2007 ◽  
Vol 367 (1) ◽  
pp. 40-48 ◽  
Author(s):  
Benlian Wang ◽  
Gang Sun ◽  
David R. Anderson ◽  
Minghong Jia ◽  
Stephen Previs ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Tandem Mass ◽  
Deuterium Incorporation ◽  
Product Ions ◽  
Low Levels ◽  
Peptide Product

scholarly journals A robust two-dimensional separation for top-down tandem mass spectrometry of the low-mass proteome

2009 ◽  
Vol 20 (12) ◽  
pp. 2183-2191 ◽  
Author(s):  
Ji Eun Lee ◽  
John F. Kellie ◽  
John C. Tran ◽  
Jeremiah D. Tipton ◽  
Adam D. Catherman ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Tandem Mass ◽  
Two Dimensional ◽  
Top Down ◽  
Low Mass

scholarly journals Analysis of Flavonoids in Dalbergia odorifera by Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry

Molecules ◽  
2020 ◽  
Vol 25 (2) ◽  
pp. 389 ◽  
Author(s):  
Xiangsheng Zhao ◽  
Shihui Zhang ◽  
Dan Liu ◽  
Meihua Yang ◽  
Jianhe Wei
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Electrospray Ionization ◽  
Negative Ion ◽  
Ionization Mass ◽  
Tandem Mass ◽  
Relative Standard ◽  
Limit Of Quantitation ◽  
Dalbergia Odorifera

Dalbergia odorifera, a traditional Chinese medicine, has been used to treat cardio- and cerebrovascular diseases in China for thousands of years. Flavonoids are major active compounds in D. odorifera. In this paper, a rapid and sensitive ultra-high performance liquid chromatography-triple quadrupole mass spectrometry method was developed and validated for simultaneous determination of 17 flavonoids in D. odorifera. Quantification was performed by multiple reaction monitoring using electrospray ionization in negative ion mode. Under the optimum conditions, calibration curves for the 17 analytes displayed good linearity (r2 > 0.9980). The intra- and inter-day precisions (relative standard deviations) were lower than 5.0%. The limit of quantitation ranged from 0.256 to 18.840 ng/mL. The mean recovery range at three spiked concentrations was 94.18–101.97%. The validated approach was successfully applied to 18 samples of D. odorifera. Large variation was observed for the contents of the 17 analytes. Sativanone and 3′-O-methylviolanone were the dominant compounds. The fragmentation behaviors of six flavonoids were investigated using UPLC with quadrupole time-of-flight tandem mass spectrometry. In negative ion electrospray ionization mass spectrometry, all the flavonoids yielded prominent [M − H]− ions. Fragments for losses of CH3, CO, and CO2 were observed in the mass spectra. Formononetin, liquiritigenin, isoliquiritigenin, sativanone, and alpinetin underwent retro-Diels–Alder fragmentations. The proposed method will be helpful for quality control of D. odorifera.

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