scholarly journals Research onTrypanosoma cruziand Analysis of Inflammatory Infiltrate in Esophagus and Colon from Chronic Chagasic Patients with and without Mega

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Eliângela de Castro Côbo ◽  
Thales Parenti Silveira ◽  
Adilha Misson Micheletti ◽  
Eduardo Crema ◽  
Sheila Jorge Adad
Keyword(s):  
T Lymphocytes ◽  
B Lymphocytes ◽  
Myenteric Plexus ◽  
Inflammatory Process ◽  
Inflammatory Infiltrate ◽  
Inflammatory Infiltration

To compare parasitism and inflammatory process in esophagus and colon from chronic chagasic patients, immunohistochemistry was carried out to research forT. cruziand to evaluate the inflammatory infiltrate in the muscular and myenteric plexus in 39 esophagi (20 with and 19 without megaesophagus) and 50 colons (25 with and 25 without megacolon). The frequency ofT. cruziin megaesophagus was 20%, and in megacolon it was 4%. No amastigotes were found in organs without mega; considering the total of esophagi (with and without mega), the frequency ofT. cruziwould be 10% and 2% in the colon. Myositis and ganglionitis were more frequent and intense in organs with mega compared to those without mega, and in esophagus compared to colon. Qualitatively, inflammatory infiltration in esophagus and colon, with or without mega, was similar, consisting predominantly of T lymphocytes (CD3+), scarce macrophages (CD68+), and rare B lymphocytes (CD20+).

scholarly journals Treatment With CD52 Antibody Protects Neurons in Experimental Autoimmune Encephalomyelitis Mice During the Recovering Phase

2021 ◽  
Vol 12 ◽  
Author(s):  
Wenlin Hao ◽  
Qinghua Luo ◽  
Michael D. Menger ◽  
Klaus Fassbender ◽  
Yang Liu
Keyword(s):  
Spinal Cord ◽  
T Lymphocytes ◽  
Experimental Autoimmune Encephalomyelitis ◽  
B Lymphocytes ◽  
Neuroprotective Effect ◽  
Recovery Phase ◽  
Conditional Knockout ◽  
Autoimmune Encephalomyelitis ◽  
Inflammatory Infiltration ◽  
Experimental Autoimmune

Multiple sclerosis (MS) is a chronic autoimmune disease driven by T and B lymphocytes. The remyelination failure and neurodegeneration results in permanent clinical disability in MS patients. A desirable therapy should not only modulate the immune system, but also promote neuroprotection and remyelination. To investigate the neuroprotective effect of CD52 antibody in MS, both C57BL/6J and SJL mice with experimental autoimmune encephalomyelitis (EAE) were treated with CD52 antibody at the peak of disease. Treatment with CD52 antibody depleted T but not B lymphocytes in the blood, reduced the infiltration of T lymphocytes and microglia/macrophages in the spinal cord. Anti-CD52 therapy attenuated EAE scores during the recovery phase. It protected neurons immediately after treatment (within 4 days) as shown by reducing the accumulation of amyloid precursor proteins. It potentially promoted remyelination as it increased the number of olig2/CC-1-positive mature oligodendrocytes and prevented myelin loss in the following days (e.g., 14 days post treatment). In further experiments, EAE mice with a conditional knockout of BDNF in neurons were administered with CD52 antibodies. Neuronal deficiency of BDNF attenuated the effect of anti-CD52 treatment on reducing EAE scores and inflammatory infiltration but did not affect anti-CD52 treatment-induced improvement of myelin coverage in the spinal cord. In summary, anti-CD52 therapy depletes CD4-positive T lymphocytes, prevents myelin loss and protects neurons in EAE mice. Neuronal BDNF regulates neuroprotective and anti-inflammatory effect of CD52 antibody in EAE mice.

Immunophenotype of the inflammatory response in the central and enteric nervous systems of cockatiels (Nymphicus hollandicus) experimentally infected with parrot bornavirus 2

2022 ◽  
pp. 030098582110691
Author(s):  
Jeann Leal de Araújo ◽  
Raquel R. Rech ◽  
Aline Rodrigues-Hoffmann ◽  
Paula R. Giaretta ◽  
Cinthya Cirqueira ◽  
...  
Keyword(s):  
Small Intestine ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Inflammatory Infiltrate ◽  
Homogeneous Distribution ◽  
Nymphicus Hollandicus ◽  
Inflammatory Infiltrates ◽  
Cellular Immune ◽  
Proventricular Dilatation Disease ◽  
The Brain

Proventricular dilatation disease is a lethal disease of psittacine birds. In this study, we characterized the local cellular immune response in the brain, proventriculus, and small intestine of 27 cockatiels ( Nymphicus hollandicus) experimentally infected with parrot bornavirus 2 (PaBV-2). Perivascular cuffs in the brain were composed of CD3+ T-lymphocytes and Iba1+ macrophages/microglia in most cockatiels (n = 26). In the ganglia of the proventriculus, CD3+ T-lymphocytes (n = 17) and Iba1+ macrophages (n = 13) prevailed. The ganglia of the small intestine had a more homogeneous distribution of these leukocytes, including PAX5+ B-lymphocytes (n = 9), CD3+ T-lymphocytes (n = 8), and Iba1+ macrophages (n = 8). Our results indicate that perivascular cuffs in the brain and the inflammatory infiltrate in the proventriculus of PaBV-2-infected cockatiels is predominately composed of T-lymphocytes, while the inflammatory infiltrates in the ganglia of the small intestine are characterized by a mixed infiltrate composed of T-lymphocytes, B-lymphocytes, and macrophages.

scholarly journals CHANGES IN THE CELLULAR IMMUNE PROTECTION OF ORGANISM IN THE PROCESS OF CHRONIZATION OF EXPERIMENTAL BACTERIAL-IMMUNE PERIODONTITIS

2018 ◽  
pp. 5-8
Author(s):  
A.Ye. Demkovych ◽  
Yu.I. Bondarenko ◽  
M.M. Yakymchuk
Keyword(s):  
T Lymphocytes ◽  
B Lymphocytes ◽  
Inflammatory Reaction ◽  
Inflammatory Process ◽  
Immune Defense ◽  
Male Rats ◽  
Natural Killers ◽  
Late Period ◽  
T Suppressors ◽  
Cellular Immune

One of the important factors that leads to damage of structures of the periodontal complex and leads to the formation of inflammatory process of varying degrees is the disruption of immunological processes. The aim of the study was to clarify the pathogenetic role of cellular adaptive immunity in the process of formation of chronic inflammatory reaction in the late period of the experimental bacterial-immune periodontitis. The study was conducted on white, non-breeding, clinically healthy male rats. Experimental bacterial-immune periodontitis in experimental animals was caused by insertion into the tissues of the periodontal complex a mixture of microorganisms diluted with egg protein. The obtained digital data was statistically processed using parametric and nonparametric statistical methods. The article represents the results of research on the parameters of cellular immune defense, determined by the relative number of CD3+ (common T-lymphocytes), CD4+ (T-helpers), CD8+ (cytotoxic cells, T-killers), CD19+ (B-lymphocytes), CD16+ (natural killers, NK-cell) and immunoregulatory index (CD4+ / CD8+) in intact animals and on the 30th day of experimental bacterial-immune periodontitis development. It was established that the nature of the course of experimental inflammation in the tissues of the periodontal complex depended on changes in the cellular immune status, accompanied by a decrease of the content of common mature T-lymphocytes (CD3+) in the blood of animals with experimental bacterial-immune periodontitis on the 30th day of the study. In the process of the development of the experimental bacterial-immune periodontitis there was a decrease of the content of T-lymphocytes-helper (CD4+) in the blood of animals and on the 30th day of the study an increase in T-suppressors (CD8+), an increase in the content of natural killers (CD16+) and a decrease in the relative content of B-lymphocytes (CD19+). The immunoregulatory index (CD4+ / СD8+) decreased in comparison with this indicator of a group of intact animals. In rats with bacterial-immune periodontitis, an immunosuppressive state developed in the late period of the inflammatory reaction due to both T-helper cells and cytotoxic T-suppressors / killers. These changes can be considered as signs of formation of the chronic course of the inflammatory process in the tissues of periodontal complex.

scholarly journals Immunopathological findings in a cat with auricular chondritis

2019 ◽  
Vol 67 (1) ◽  
pp. 81-86 ◽  
Author(s):  
Tais Meziara Wilson ◽  
Mizael Machado ◽  
Davi Emanuel Ribeiro De Sousa ◽  
Tainã Braúna ◽  
Rafael Torres Neto ◽  
...  
Keyword(s):  
Mast Cells ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Inflammatory Infiltrate ◽  
Morphological Changes ◽  
Relapsing Polychondritis ◽  
Granulomatous Inflammation ◽  
Inflammatory Lesion ◽  
Auricular Chondritis ◽  
Mixed Inflammatory Infiltrate

At clinical examination, a 5-year-old male domestic short-haired cat exhibited painful swelling and erythema of the pinnae of both ears. Microscopically, the lesions on both pinnae were composed of diffuse granulomatous chondritis with degeneration and necrosis of the pinnal cartilage. Numerous mast cells were also observed within and surrounding the inflammatory lesion. Immunohistochemistry showed a mixed inflammatory infiltrate characterised by the predominance of macrophages (CD68+, MAC 387+ and Lysozyme+), T lymphocytes (CD3+), some B lymphocytes (CD79α+) and neutrophils. Immunopathological characterisation of the lesion showed a granulomatous inflammation profile and suggests that the morphological changes and immunopathogenesis of auricular chondritis in cats presents a similarity with relapsing polychondritis in humans.

scholarly journals High levels ofbcl-2 protein in circulating t lymphocytes, but not b lymphocytes, of patients with systemic lupus erythematosus

1994 ◽  
Vol 37 (10) ◽  
pp. 1423-1430 ◽  
Author(s):  
Martin Aringer ◽  
Winfried Wintersberger ◽  
Carl W. Steiner ◽  
Hans Kiener ◽  
Elisabeth Presterl ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Lupus Erythematosus ◽  
Systemic Lupus

scholarly journals T lymphocyte-dependent B lymphocyte proliferative response to antigen. I Genetic restriction of the stimulation of B lymphocyte proliferation.

1981 ◽  
Vol 153 (4) ◽  
pp. 871-882 ◽  
Author(s):  
H Y Tse ◽  
J J Mond ◽  
W E Paul
Keyword(s):  
T Lymphocytes ◽  
B Lymphocytes ◽  
T Lymphocyte ◽  
Lymphocyte Proliferation ◽  
B Lymphocyte ◽  
Antigen Specificity ◽  
Apparent Lack ◽  
The Face ◽  
Stimulation Of

For the purpose of examining more closely the interaction between T and B lymphocytes, we have developed an in vitro T lymphocyte-dependent B lymphocyte proliferation assay. Proliferation of B lymphocytes in response to antigen was found to depend on the presence of primed T lymphocytes; the B lymphocytes could be derived from nonprimed animals. It appears that these B cells were nonspecifically recruited to proliferate. This nonspecific recruitment, however, was found to be Ir-gene restricted in that B lymphocytes from B10.S mice, which are genetic nonresponders to the polymer Glu60-Ala30-Tyr10 (GAT), could not be stimulated by GAT-primed (responder X nonresponder) F1 T cells. The apparent lack of antigen specificity in the face of Ir gene-restricted T-B interaction may have important implications in our understanding of the recognition unit(s) on T lymphocytes.

scholarly journals DC-SIGN on B Lymphocytes Is Required For Transmission of HIV-1 to T Lymphocytes

2006 ◽  
Vol 2 (7) ◽  
pp. e70 ◽  
Author(s):  
Giovanna Rappocciolo ◽  
Paolo Piazza ◽  
Craig L Fuller ◽  
Todd A Reinhart ◽  
Simon C Watkins ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
B Lymphocytes ◽  
Hiv 1

scholarly journals Correction: DC-SIGN on B Lymphocytes Is Required for Transmission of HIV-1 to T Lymphocytes

2006 ◽  
Vol 2 (8) ◽  
pp. e88 ◽  
Author(s):  
Giovanna Rappocciolo ◽  
Paolo Piazza ◽  
Craig L. Fuller ◽  
Todd A. Reinhart ◽  
Simon C. Watkins ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
B Lymphocytes ◽  
Hiv 1

scholarly journals Activity of the T- and B-system of the cell immunity of animals under conditions of oxidation stress and effects of the liposomal drug

2017 ◽  
Vol 7 (4) ◽  
pp. 536-541 ◽  
Author(s):  
M. Khariv ◽  
B. Gutyj ◽  
N. Ohorodnyuk ◽  
O. Vishchur ◽  
I. Khariv ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Cellular Immunity ◽  
Relative Number ◽  
The Body ◽  
T And B Lymphocytes ◽  
Oxidation Stress ◽  
Liposomal Preparation ◽  
Experimental Group

The results of research on the influence of the elaborated complex immunotropic drug containing butafosfan, interferon, thistle and fat-soluble vitamins A, D3, E in the form of a liposomal emulsion on the activity of T- and B-cell chains of immunity in rats under the conditions of action on the body of oxidative stress are presented. It has been established that the introduction of 50% tetrachloromethane into the rats of the first and second experimental groups, with a dose of 0.25 ml per 100 g of body weight, causes oxidative stress in them which negatively affects the cellular immunity and functional activity of immunocompetent blood cells. Immunosuppressive effects of oxidative stress were manifested by a decrease in the blood of rats in the first and second experimental groups of the number of T- and B-lymphocytes and their regulatory subpopulations mainly on the 2nd and 5th day of the study. At the same time, in the blood of rats of the first experimental group in all research periods a decrease in the relative number of common, active and theophylline-resistant T-lymphocytes, as well as B-lymphocytes was observed with a noticeable increase in the number of their undifferentiated forms. At the same time, the obtained data suggest the positive effect of butafosfan, interferon, thistle and vitamins A, D3, E in the liposomal preparation on the relative amount of T- and B-lymphocytes and on the redistribution of avidity in the direction of strengthening the receptor field of plasma membranes immunocompetent cells. It was found that the normalization on the 2nd day of blood level in the second experimental group of common T-lymphocytes occurred due to the secondary forms of the blood and active T-lymphocytes by changes in the number of low-avid forms. In addition to the indicators characterizing the cellular immunity of rats, the components of the liposomal preparation showed regulatory influence on the humoral link of the immune response. In particular, on the 10th day of research in blood of rats of the second experimental group a tendency towards an increase in the relative number of B-lymphocytes and an increase in the number of cells with low and medium density of receptors was found, which, under the conditions of oxidative stress, indicates an increase in the body's ability to actively synthesize protective antibodies

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