scholarly journals Cell Proliferation Tracking Using Graphene Sensor Arrays

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Ronan Daly ◽  
Shishir Kumar ◽  
Gyongyi Lukacs ◽  
Kangho Lee ◽  
Anne Weidlich ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Proliferation ◽  
Drug Development ◽  
Lead Time ◽  
Sensor Array ◽  
Sensor Arrays ◽  
Device Fabrication ◽  
Label Free ◽  
Free Graphene ◽  
Graphene Fet

The development of a novel label-free graphene sensor array is presented. Detection is based on modification of graphene FET devices and specifically monitoring the change in composition of the nutritive components in culturing medium. Micro-dispensing ofEscherichia coliin medium shows feasibility of accurate positioning over each sensor while still allowing cell proliferation. Graphene FET device fabrication, sample dosing, and initial electrical characterisation have been completed and show a promising approach to reducing the sample size and lead time for diagnostic and drug development protocols through a label-free and reusable sensor array fabricated with standard and scalable microfabrication technologies.

Label-free detection and discrimination of poly-brominated diphenylethers using molecularly imprinted photonic cross-reactive sensor arrays

2014 ◽  
Vol 50 (91) ◽  
pp. 14133-14136 ◽  
Author(s):  
Dan Xu ◽  
Wei Zhu ◽  
Chen Wang ◽  
Tian Tian ◽  
Jian Li ◽  
...  
Keyword(s):  
Sensor Array ◽  
Sensor Arrays ◽  
Label Free ◽  
Molecularly Imprinted ◽  
Label Free Detection

Molecularly imprinted photonic polymers were used as sensing elements for efficiently creating a cross-reactive sensor array, as well as the sensitive and label-free detection of trace PBDEs was realized.

PSA detection using label free graphene FET with coplanar electrodes based microfluidic point of care diagnostic device

Talanta ◽  
2021 ◽  
Vol 222 ◽  
pp. 121581
Author(s):  
Naresh Mandal ◽  
Victor Pakira ◽  
Nirmalya Samanta ◽  
Naren Das ◽  
Suman Chakraborty ◽  
...  
Keyword(s):  
Point Of Care ◽  
Label Free ◽  
Diagnostic Device ◽  
Free Graphene ◽  
Coplanar Electrodes ◽  
Graphene Fet

scholarly journals Rapid label-free detection of E. coli using antimicrobial peptide assisted impedance spectroscopy

2015 ◽  
Vol 7 (23) ◽  
pp. 9744-9748 ◽  
Author(s):  
Keren Jiang ◽  
Hashem Etayash ◽  
Sarfuddin Azmi ◽  
Selvaraj Naicker ◽  
Mahtab Hassanpourfard ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Impedance Spectroscopy ◽  
Antimicrobial Peptide ◽  
Rapid Detection ◽  
Sensor Array ◽  
Label Free ◽  
E Coli ◽  
Sensor Platform ◽  
Label Free Detection

A compact, label-free sensor array for rapid detection ofEscherichia coli(E. coli) using antimicrobial peptide assisted impedimetric sensor platform.

scholarly journals Label-free quantitative proteomic analysis of the inhibition effect of Lactobacillus rhamnosus GG on Escherichia coli biofilm formation in co-culture

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Huiyi Song ◽  
Ni Lou ◽  
Jianjun Liu ◽  
Hong Xiang ◽  
Dong Shang
Keyword(s):  
Escherichia Coli ◽  
Proteomic Analysis ◽  
Biofilm Formation ◽  
Lactobacillus Rhamnosus ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Label Free ◽  
Quantitative Proteomic Analysis ◽  
E Coli ◽  
Protein Ubiquitination

Abstract Background Escherichia coli (E. coli) is the principal pathogen that causes biofilm formation. Biofilms are associated with infectious diseases and antibiotic resistance. This study employed proteomic analysis to identify differentially expressed proteins after coculture of E. coli with Lactobacillus rhamnosus GG (LGG) microcapsules. Methods To explore the relevant protein abundance changes after E. coli and LGG coculture, label-free quantitative proteomic analysis and qRT-PCR were applied to E. coli and LGG microcapsule groups before and after coculture, respectively. Results The proteomic analysis characterised a total of 1655 proteins in E. coli K12MG1655 and 1431 proteins in the LGG. After coculture treatment, there were 262 differentially expressed proteins in E. coli and 291 in LGG. Gene ontology analysis showed that the differentially expressed proteins were mainly related to cellular metabolism, the stress response, transcription and the cell membrane. A protein interaction network and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis indicated that the differentiated proteins were mainly involved in the protein ubiquitination pathway and mitochondrial dysfunction. Conclusions These findings indicated that LGG microcapsules may inhibit E. coli biofilm formation by disrupting metabolic processes, particularly in relation to energy metabolism and stimulus responses, both of which are critical for the growth of LGG. Together, these findings increase our understanding of the interactions between bacteria under coculture conditions.

scholarly journals Anemoside B4 ameliorates TNBS-induced colitis through S100A9/MAPK/NF-κB signaling pathway

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Yong Zhang ◽  
Zhengxia Zha ◽  
Wenhua Shen ◽  
Dan Li ◽  
Naixin Kang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Signaling Pathway ◽  
Tca Cycle ◽  
Mapk Signaling ◽  
Enzyme Linked Immunosorbent Assay ◽  
Trinitrobenzene Sulfonic Acid ◽  
Therapeutic Effects ◽  
Triterpenoid Saponin ◽  
Label Free

Abstract Background Despite the increased morbidity of ulcerative colitis (UC) in the developing countries, available treatments remain unsatisfactory. Therefore, it is urgent to discover more effective therapeutic strategies. Pulsatilla chinensis was widely used for the treatment of inflamed intestinal diseases including UC for thousands of years in China. Anemoside B4, the most abundant triterpenoid saponin isolated from P. chinensis, exerts anti-inflammatory and antioxidant effects and may be the most active compounds, which is responsible for the therapeutic effects. However, the mechanism how anemoside B4 executes its biological functions is still elusive. Methods Here, we used the 2, 4, 6-trinitrobenzene sulfonic acid (TNBS)-induced colitis rat model to evaluate the therapeutic effect of anemoside B4. Blood samples of colitis rats were collected for hematology analysis. The inflammation-associated factors were investigated by enzyme-linked immunosorbent assay (ELISA). Cell proliferation and apoptosis was determined with EdU cell proliferation assay and TUNEL assay. The proteins regulated by anemoside B4 were identified by label-free quantitative proteomics. The significantly down-regulated proteins were verified by Western blotting analysis. mRNA expression was analyzed by quantitative real-time RT-PCR. Results The results showed that anemoside B4 ameliorated TNBS-induced colitis symptoms, including tissue damage, inflammatory cell infiltration, and pro-inflammatory cytokine production, apoptosis and slowed proliferation in colon. Quantitative proteomic analyses discovered that 56 proteins were significantly altered by anemoside B4 in the TNBS-induced rats. These proteins mainly clustered in tricarboxylic acid (TCA) cycle and respiratory electron transport chain. Among the altered proteins, S100A9 is one of the most significantly down-regulated proteins and associated with NF-κB and MAPK signaling pathways in the pathogenesis of UC. Further experiments revealed that anemoside B4 suppressed the expression of S100A9 and its downstream genes including TLR4 and NF-κB in colon. In vitro, anemoside B4 could inhibit the NF-κB signaling pathway induced by recombinant S100A9 protein in human intestinal epithelial Caco-2 cells. Moreover, anemoside B4 inhibits neutrophils recruitment and activation in colon induced by TNBS. Conclusions Our results demonstrate that anemoside B4 prevents TNBS-induced colitis by inhibiting the NF-κB signaling pathway through deactivating S100A9, suggesting that anemoside B4 is a promising therapeutic candidate for colitis.

Label-Free Detection of Escherichia coli from Mixed Bacterial Cultures Using Bacteriophage T4 on Plasmonic Fiber-Optic Sensor

ACS Sensors ◽  
2021 ◽  
Author(s):  
Pallavi Halkare ◽  
Nirmal Punjabi ◽  
Jigme Wangchuk ◽  
Santhosh Madugula ◽  
Kiran Kondabagil ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fiber Optic ◽  
Bacteriophage T4 ◽  
Fiber Optic Sensor ◽  
Label Free ◽  
Bacterial Cultures ◽  
Optic Sensor ◽  
Label Free Detection

scholarly journals Label-Free Electrochemical Biosensor Based on Au@MoS₂–PANI for Escherichia coli Detection

Chemosensors ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 49
Author(s):  
Pushap Raj ◽  
Man Hwan Oh ◽  
Kyudong Han ◽  
Tae Yoon Lee
Keyword(s):  
Escherichia Coli ◽  
Bacterial Infections ◽  
Limit Of Detection ◽  
Bacterial Pathogens ◽  
Cost Effective ◽  
Covalent Immobilization ◽  
Label Free ◽  
Self Assembled Monolayer ◽  
Detection Range ◽  
Linear Detection

Bacterial infections have become a significant challenge in terms of public health, the food industry, and the environment. Therefore, it is necessary to address these challenges by developing a rapid, cost-effective, and easy-to-use biosensor for early diagnosis of bacterial pathogens. Herein, we developed a simple, label-free, and highly sensitive immunosensor based on electrochemical detection using the Au@MoS₂–PANI nanocomposite. The conductivity of the glassy carbon electrode is greatly enhanced using the Au@MoS₂–PANI nanocomposite and a self-assembled monolayer of mercaptopropionic acid on the gold nanoparticle surface was employed for the covalent immobilization of antibodies to minimize the nonspecific adsorption of bacterial pathogens on the electrode surface. The biosensor established a high selectivity and sensitivity with a low limit of detection of 10 CFU/mL, and detected Escherichia coli within 30 min. Moreover, the developed biosensor demonstrated a good linear detection range, practical utility in urine samples, and electrode regenerative studies.

Fundamental Sensor Development in Electrical Resistance Tomography

2015 ◽  
Vol 73 (6) ◽  
Author(s):  
Ling En Hong ◽  
Ruzairi Hj. Abdul Rahim ◽  
Anita Ahmad ◽  
Mohd Amri Md. Yunus ◽  
Khairul Hamimah Aba ◽  
...  
Keyword(s):  
Image Reconstruction ◽  
Data Acquisition ◽  
Electrical Resistance ◽  
Sensor Array ◽  
Sensor Arrays ◽  
Region Of Interest ◽  
Display System ◽  
Electrical Resistance Tomography ◽  
Fundamental Understanding ◽  
Collection Strategies

This paper will provide a fundamental understanding of one of the most commonly used tomography, Electrical Resistance Tomography (ERT). Unlike the other tomography systems, ERT displayed conductivity distribution in the Region of Interest (ROI) and commonly associated to Sensitivity Theorem in their image reconstruction. The fundamental construction of ERT includes a sensor array spaced equally around the imaged object periphery, a Data Acquisition (DAQ), image reconstruction and display system. Four ERT data collection strategies that will be discussed are Adjacent Strategy, Opposite Strategy, Diagonal Strategy and Conducting Boundary Strategy. We will also explain briefly on some of the possible Data Acquisition System (DAQ), forward and inverse problems, different arrangements for conducting and non-conducting pipes and factors that influence sensor arrays selections. 

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