scholarly journals Evaluation of an Experimental Gel ContainingEuclea natalensis: AnIn VitroStudy

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Sílvia Helena de Carvalho Sales-Peres ◽  
Letícia Ferreira de Freitas Brianezzi ◽  
Juliane Avansini Marsicano ◽  
Moacir Rossi Forim ◽  
Maria Fatima das Graças Fernandes da Silva ◽  
...  
Keyword(s):  
Fluid Flow ◽  
Citric Acid ◽  
Promising Result ◽  
Clinical Situation ◽  
Third Molars ◽  
Dentin Permeability ◽  
Flow Apparatus ◽  
Euclea Natalensis ◽  
Acid Challenge ◽  
Gel Ph

Objective. To evaluate the effect of an experimental gel containingEuclea natalensisextract on dentin permeability.Methods. Thirty-six dentin discs, 1-mm-thick. The discs were prepared from the coronal dentin of extracted human third molars that were divided into 3 groups (). The dentin discs in each group were treated with the groups following experimental materials: (FG): 1.23% fluoride gel, pH 4.1; (EG):Euclea natalensisextract gel, pH 4.1; (CG): control gel, pH 4.1. The gels were applied to the occlusal slide of the dentin under the following conditions: after 37% phosphoric acid and before 6% citric acid. The hydraulic conductance (HC) of each condition was determined four times using a fluid flow apparatus (Flodec). The data were analyzed using Two-way ANOVA and Tukey's test ().Results. The greatest mean reduction in HC was produced in group EG dentin discs (61.2%; ). Even after acid challenge with 6% citric acid the great reduction occurred in group EG (66.0%; ) than other groups (CG-77.1%, FG-90.8%).Conclusion.E. natalensisgel not only reduced dentin permeability, but also resisted posttreatment citric acid challenge without changing its permeability. Further research has to confirm this promising result in the clinical situation.

scholarly journals Effect of iron gel on dentin permeability

2011 ◽  
Vol 22 (3) ◽  
pp. 198-202 ◽  
Author(s):  
Sílvia Helena de Carvalho Sales-Peres ◽  
João Victor Donazan Reinato ◽  
André de Carvalho Sales-Peres ◽  
Juliane Avansini Marsicano
Keyword(s):  
Hydraulic Conductance ◽  
Smear Layer ◽  
Iron Sulfate ◽  
Dentin Hypersensitivity ◽  
Third Molars ◽  
Potassium Oxalate ◽  
Significance Level ◽  
Dentin Permeability ◽  
Gel Ph

This study evaluated in vitro the effect of an experimental gel containing iron on the reduction of hydraulic conductance of dentin. Thirty-six 1-mm-thick dentin discs obtained from extracted human third molars were divided into 3 groups of 10 specimens each. The groups corresponded to the following experimental materials: 1.23% acidulated phosphate fluoride gel, pH 4.1; 3% potassium oxalate gel, pH 4.1 (Oxa-Gel®); and iron sulfate gel (10 mmol/L FeSO4), pH 4.1. The gels were applied to dentin under the following conditions: after 37% phosphoric acid and before 6% citric acid. Data were analyzed statistically by ANOVA and Tukey's test at 5% significance level. There was no significant differences (p<0.05) among the groups in any of the conditions for hydraulic conductance reduction, except for smear layer presence. The active agents reduced dentin permeability, but they produced significantly lower (p<0.05) reduction in hydraulic conductance when compared to presence of smear layer. The effectiveness in reducing dentin permeability was not significantly different (p>0.05) among the gels. This study suggests that the iron gel promoted reduction in dentin permeability comparable to that of the other agents and thus may be considered a good clinical alternative for the treatment of dentin hypersensitivity.

Effect of Conventional and Acid-modified Casein Phosphopeptide-Amorphous Calcium Phosphate Crèmes on Dentin Permeability Before and After Acid Challenge

2019 ◽  
Vol 44 (5) ◽  
pp. 530-535
Author(s):  
K Kijsamanmith ◽  
D Banomyong ◽  
MF Burrow ◽  
P Kanchanasantikul ◽  
S Wipawiwat ◽  
...  
Keyword(s):  
Fluid Flow ◽  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Dentinal Tubule ◽  
Dentinal Tubules ◽  
Dentin Permeability ◽  
Before And After ◽  
Casein Phosphopeptide ◽  
Partially Occluded ◽  
Acid Challenge

SUMMARY This study investigated the effect of conventional and acidified casein phosphopeptide-amorphous calcium phosphate–containing crèmes (CPP-ACP and ACPP-ACP) on dentin permeability (DP) before and after acid challenge, using a fluid-flow measuring device and scanning electron microscopic (SEM) examination. Fifty dentin discs were prepared from intact human third molars. Each tooth was sectioned 3 mm above and below the cementoenamel junction. The smear layer was removed to expose the dentin and dentinal tubules. For fluid-flow measurement, 20 specimens were used and divided into two groups: (a) CPP-ACP (pH=7) and (b) ACPP-ACP (pH=2). The dentin surfaces were treated with the crème for five minutes daily for two weeks, and then an acid challenge was performed using 6% citric acid. Under simulated pulpal pressure, DP was measured at baseline, five minutes, two weeks, and after acid challenge. The remaining specimens were prepared and examined using SEM to investigate dentinal tubule occlusion. DP was not significantly different between the two CPP-ACP–containing crèmes at any test period (p≥0.05). DP after two weeks or acid challenge was significantly lower than that at five minutes (p&lt;0.05). The results were consistent with the SEM micrographs that presented patent dentinal tubules after a five-minute application and partial blockage of dentinal tubules after the two-week application. The dentinal tubules remained partially occluded after acid challenge. Using conventional and acid-modified CPP-ACP–containing crèmes for two weeks decreased DP and partially occluded the dentinal tubules, which resisted acid challenge.

scholarly journals Effect of experimental moringa and propolis toothpastes on surface microhardness of simulated hypersensitive dentin

2021 ◽  
Vol 45 (1) ◽  
Author(s):  
Hoda R. Riad ◽  
Osama S. Mosallam ◽  
Alaa H. Salama ◽  
Lamiaa M. Moharam ◽  
Mohamed H Zaazou ◽  
...  
Keyword(s):  
Citric Acid ◽  
Artificial Saliva ◽  
Partial Recovery ◽  
Surface Microhardness ◽  
Dentin Surface ◽  
Ph Cycling ◽  
Electric Toothbrush ◽  
Post Hoc ◽  
One Way Anova ◽  
Acid Challenge

Abstract Objective Objective was to investigate the effect of NovaMin toothpaste and two experimental toothpastes on surface microhardness of hypersensitive dentin. Methods Eighty specimens were prepared and divided randomly into eight groups (n = 10/group) according to the tested toothpastes (NovaMin, moringa and propolis toothpastes) and the acidic challenge (with or without 6% citric acid challenge). Enamel surfaces were removed, and the dentin surfaces were exposed on which the three tested toothpastes were brushed twice a day for 1 week using an electric toothbrush, then a 4-d citric acid cycling model with pH 5 was performed. The prepared specimens were stored in artificial saliva until the microhardness test was performed. Statistical analysis was done using One-way ANOVA followed by Tukey post hoc test. Results NovaMin-containing toothpaste showed an increased dentin surface microhardness following a week of twice daily brushing compared to other groups. However, NovaMin-containing toothpaste showed the highest microhardness values following pH cycling with citric acid of pH 5 compared to other tested groups. Conclusions NovaMin-containing toothpaste showed a partial recovery of dentin microhardness at pH 5 during the acidic challenge. Moreover, propolis- and moringa-containing toothpastes had an insignificant effect on dentin surface microhardness with and without citric acid challenge.

scholarly journals Assessment of the acid resistance behaviour of dentin tubules occluded by different desensitizers

2020 ◽  
Vol 10 (3) ◽  
pp. 80-89
Author(s):  
Fatih Karayürek ◽  
Osman Fatih Arpağ
Keyword(s):  
Citric Acid ◽  
Native Speakers ◽  
Acid Resistance ◽  
Saline Control ◽  
Potassium Oxalate ◽  
Home Based ◽  
Native Speakers Of English ◽  
Dentin Tubules ◽  
Dentinal Hypersensitivity ◽  
Acid Challenge

Aim: To evaluate the efficacy of in-office and home-based products in the prevention of dentinal hypersensitivity on dentin discs. Methodology: Ninety-six dentin disc samples of 1 mm were divided equally into six groups as follows: TeethmateTM, Smartprotect®, novamin®, Arginine calcium carbonate, potassium oxalate with rinse and saline (control). After acid etching, test agents were applied, and eight samples in each group were subjected to citric acid. SEM examination (3000x) and statistical analysis were carried out. Results: Before citric acid challenge, novamin® and teethmate had superiority when compared to others (p<0.001), but there was no a statistical difference between novamin® and teethmate. After citric acid challenge, teethmate and smartprotect showed resistance on occluded tubules. The decreasing in the level of occluded tubules in novamin® group was statistically significant (p<0.05). In-office products including teethmate and smartprotect were found more successful compared to home-based products. Smartprotect resisted against to citric acid (0.506). Conclusion: Novamin® showed a successful occlusion in all tubules before acid challenge whereas; it was the most unsuccessful group after acid challenge when compared to other agents. In-office agents were found to be more successful in reducing dentine permeability than home-care products. In-office agents showed more resistance to acid attacks. Further studies are needed to assess the efficacy of desensitizer agents. How to cite this article: Karayürek F, Arpağ OF. Assessment of the acid resistance behaviour of dentin tubules occluded by different desensitizers. Int Dent Res 2020;10(3):80-9.  https://doi.org/10.5577/intdentres.2020.vol10.no3.3   Linguistic Revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English.

scholarly journals Novel Pastes Containing Polymeric Nanoparticles for Dentin Hypersensitivity Treatment: An In Vitro Study

Nanomaterials ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3150
Author(s):  
Manuel Toledano-Osorio ◽  
Raquel Osorio ◽  
Estrella Osorio ◽  
Antonio L. Medina-Castillo ◽  
Manuel Toledano
Keyword(s):  
Mechanical Properties ◽  
Citric Acid ◽  
Complex Modulus ◽  
Polymeric Nanoparticles ◽  
In Vitro Study ◽  
Dentin Hypersensitivity ◽  
Dentinal Tubule ◽  
Tubule Occlusion ◽  
Acid Challenge

Tubule occlusion and remineralization are considered the two main goals of dentin hypersensitivity treatment. The objective is to assess the ability of dentifrices containing zinc-doped polymeric nanoparticles (NPs) to enduringly occlude the dentinal tubules, reinforcing dentin’s mechanical properties. Fifteen dentin surfaces were acid-treated for dentinal tubule exposure and brushed with (1) distilled water, or with experimental pastes containing (2) 1% of zinc-doped NPs, (3) 5% of zinc-doped NPs, (4) 10% of zinc-doped NPs or (5) Sensodyne®. Topographical and nanomechanical analyses were performed on treated dentin surfaces and after a citric acid challenge. ANOVA and Student–Newman–Keuls tests were used (p < 0.05). The main results indicate that all pastes produced tubule occlusion (100%) and reinforced mechanical properties of intertubular dentin (complex modulus was above 75 GPa). After the citric acid challenge, only those pastes containing zinc-doped NPs were able to maintain tubular occlusion, as specimens treated with Sensodyne® have around 30% of tubules opened. Mechanical properties were maintained for dentin treated with Zn-doped NPs, but in the case of specimens treated with Sensodyne®, complex modulus values were reduced below 50 GPa. It may be concluded that zinc-doped NPs at the lowest tested concentration produced acid-resistant tubular occlusion and increased the mechanical properties of dentin.

scholarly journals Dentin Sealing and Bond Strength Evaluation of Hema-Free and Multi-Mode Adhesives to Biomodified Dentin

2017 ◽  
Vol 28 (6) ◽  
pp. 731-737 ◽  
Author(s):  
Renata Bacelar-Sá ◽  
Marcelo Giannini ◽  
Glaucia Maria Bovi Ambrosano ◽  
Ana Karina Bedran-Russo
Keyword(s):  
Bond Strength ◽  
Water Storage ◽  
Grape Seed ◽  
Control Group ◽  
Third Molars ◽  
Untreated Control Group ◽  
Dentin Permeability ◽  
Adhesive Application ◽  
Maximum Permeability ◽  
Multi Mode

Abstract This study evaluated the effect of dentin biomodification on the bond strength (BS) and sealing ability (SA) of HEMA-free and multi-mode adhesives after 24 h and 6 months of water storage. Four adhesives were tested: two multi-mode (Scotchbond Universal - SU, and Prime & Bond Elect - PB) and two HEMA-free (All-Bond 3 - AB, and G-Aenial - GA). Human third molars were selected and dentin was treated with two cross-linking agents (5% glutaraldehyde and 6.5% proanthocyanidin-rich grape seed extract - PACs) for 10 min or kept untreated (control group) (n=6). Teeth were sectioned and prepared for BS test and SA analysis. The SA measurements were taken with the presence of smear layer (minimum permeability), EDTA treatment (maximum permeability), PACs application, adhesive application and after 6 months of water storage. BS data were analyzed by Proc Mixed and Tukey-Kramer test (α=5%). PACs application increased the BS for all adhesives tested at 24 h. However, BS decreased for SU and AB after six months. In general, multi-mode adhesives (SU and PB) did not differ from AB HEMA-free. GA presented the lowest BS values at both times of evaluation. Dentin permeability was reduced after PACs application and remained the same after 6 months, regardless adhesive application. PACs can increase the BS regardless the type of adhesive, however only for PB and GA the BS kept stable after 6-months of water storage. PACs was able to seal the dentin as the minimum permeability and also remained stable after 6 months.

Dentin Permeability and Nanoleakage of Universal Adhesives in Etch-and-rinse vs Self-etch Modes

2021 ◽  
Author(s):  
JMMS Cruz ◽  
ALGBM Silva ◽  
RMRF Eira ◽  
BMPR Sousa ◽  
MMN Lopes ◽  
...  
Keyword(s):  
Adhesive System ◽  
Third Molars ◽  
Adhesive Systems ◽  
Dentin Permeability ◽  
Transmission Electron ◽  
Mode Of Application ◽  
Etch And Rinse ◽  
Universal Adhesives ◽  
Self Etch ◽  
Ammoniacal Silver

Clinical Relevance Universal adhesives do not seem to have the ability to effectively seal dentin, regardless of the mode of application. SUMMARY Purpose: This study evaluates the reduction in dentin permeability (P) and the expression of nanoleakage in resin-dentin interfaces made with universal adhesives, comparing the self-etch mode of application with the etch-and-rinse mode of application. Methods and Materials: To measure dentin P at the baseline and after adhesive polymerization, 80 extracted noncarious human third molars (N=80) were sectioned to obtain 0.7-mm-thick midcoronal dentin disks. The specimens were randomly assigned to 8 groups according to the different adhesive systems and application modes used: Scotchbond Universal (SBU; etch-and-rinse [ER] mode vs self-etch [SE] mode), OptiBond XTR (OPT; etch-and-rinse mode vs self-etch mode), Clearfil Universal Bond Quick (CL; etch-and-rinse mode vs self-etch mode), and Adhese Universal (ADH; etch-and-rinse mode vs self-etch mode). A two-way analysis of variance with interaction was used to evaluate the P reduction differences among the groups (α=0.05). Sixteen additional 0.7-mm-thick dentin disks were similarly prepared and treated, immersed in 50 wt% ammoniacal silver nitrate, and then processed according to conventional methods for analysis under transmission electron microscopy. Results: The differences in P reduction among the adhesives were significant (p=0.029). For OPT and ADH (p=0.0304 and p=0.0120, respectively), the P reduction was higher in the ER mode than in the SE mode. For CL and SBU, no differences in P reduction was observed between the two application modes (p&gt;0.05). All the groups showed some pattern of nanoleakage. Conclusions: No adhesive system completely reduced dentin P. Dentin sealing was higher for OPT and ADH in the ER mode, while for SBU and CL, there was no difference between the two modes. All the adhesive systems showed nanoleakage.

scholarly journals In Vitro Evaluation of Desensitizing Agents Containing Bioactive Scaffolds of Nanofibers on Dentin Remineralization

Materials ◽  
2021 ◽  
Vol 14 (5) ◽  
pp. 1056
Author(s):  
Natália Bastos-Bitencourt ◽  
Marilia Velo ◽  
Tatiana Nascimento ◽  
Cassiana Scotti ◽  
Maria Gardennia da Fonseca ◽  
...  
Keyword(s):  
Statistical Significance ◽  
The Other ◽  
Fluoride Varnish ◽  
Confocal Laser ◽  
Dentinal Tubules ◽  
Bioactive Scaffolds ◽  
Dentin Permeability ◽  
Desensitizing Agents ◽  
Acid Challenge

This study evaluated the effect of the incorporation of bioactive nanofibers in desensitizing agents on dentin permeability. Sixty disks of dentin were randomly distributed in four groups (n = 15). Distribution was based on the desensitizing agents, fluoride varnish and self-etching adhesive, and the presence of nanofibers: C (self-etching adhesive Clearfil SE Bond), CN (Clearfil SE Bond with 1% nanofiber), D (Duraphat varnish), and DN (Duraphat varnish with 1% nanofiber). Dentin permeability was determined using hydraulic conductivity. For a qualitative analysis, confocal laser microscopy and scanning electron microscopy were performed. The C group showed the lowest hydraulic conductance (Lp%) (89.33), while the DN group showed the highest Lp% (116.06). No statistical significance was observed in the Lp% values in all groups after the treatment and 6% citric acid challenge (p > 0.239). In the images, the CN group presented a higher superficial and intratubular deposition. In addition, this group presented a more homogeneous dentin surface and wide occlusion of dentinal tubules than the other treatments. Despite there being no statistical differences among the treatments employed, the images showed that the CN group presented a higher surface and intratubular deposition compared to the other treatments, even after the acid challenge.

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