scholarly journals Development and Validation of a Reversed-Phase Liquid Chromatography Method for the Simultaneous Determination of Indole-3-Acetic Acid, Indole-3-Pyruvic Acid, and Abscisic Acid in Barley (Hordeum vulgareL.)

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Ilva Nakurte ◽  
Anete Keisa ◽  
Nils Rostoks
Keyword(s):  
Acetic Acid ◽  
Abscisic Acid ◽  
Liquid Chromatography ◽  
Pyruvic Acid ◽  
Limit Of Detection ◽  
Solid Phase ◽  
Reversed Phase ◽  
Validation Parameters ◽  
Indole 3 Acetic Acid

A simple, sensitive, precise, and specific reverse HPLC method was developed and validated for the determination of plant hormones in barley (Hordeum vulgareL.). The method includes extraction in aqueous organic solvent followed by solid-phase extraction, sample evaporation, and reversed-phase HPLC analysis in a general purpose UV-visible (abscisic acid (ABA)) and fluorescence detection (indole-3-acetic acid (IAA) and indole-3-pyruvic acid (IPA)), high-performance liquid chromatography system. The separation was carried out on Zorbax Eclipse XDB C8 column (150 × 4.6 mm I.D) with a mobile phase composed of methanol and 1% acetic acid (60 : 40 v/v) in isocratic mode at a flow rate of 1 ml min-1. The detection was monitored at 270 nm (ABA) and at 282 nm (Ex) and 360 nm (Em) (IAA, IPA). The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantification, and robustness. The determined validation parameters are in the commonly acceptable ranges for that kind of analysis.

scholarly journals Determination of Total Avermectin B1 and 8,9-Z-Avermectin B1 Residues in Wine by Liquid Chromatography

1996 ◽  
Vol 79 (5) ◽  
pp. 1158-1161 ◽  
Author(s):  
Janice A Cobin ◽  
Nelson A Johnson
Keyword(s):  
Liquid Chromatography ◽  
Limit Of Detection ◽  
Solid Phase ◽  
Signal To Noise Ratio ◽  
Reversed Phase ◽  
Liquid Chromatographic Method ◽  
Limit Of Quantitation ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract A liquid chromatographic method was developed and validated for determination of avermectin Bi and 8,9-Z-avermectin B1 residues in wine. The sample is extracted with hexane-acetonitrile and the hexane layer containing the avermectins is concentrated/ purified on an aminopropyl solid-phase extraction (SPE) column. The purified extract is derivatized with trifluoroacetic anhydride and the derivatized avermectins are analyzed by reversed-phase liquid chromatography with fluorescence detection. Recoveries of avermectins from wine fortified with approximately 1-25 ng/g avermectin B1a or 8,9-Zavermectin B1a averaged 88 and 102%, respectively. The limit of quantitation is 1 ng/g (signal-to-noise ratio [S/N] > 10) and the limit of detection is 0.5 ng/g (S/N > 3) for each analyte. This procedure provides a simple, rapid, and sensitive method for monitoring the total amount of avermectin residues in wine.

scholarly journals Optimized High-Performance Liquid Chromatography Method for Determining Nine Cytokinins, Indole-3-acetic Acid and Abscisic Acid

2021 ◽  
Vol 13 (13) ◽  
pp. 6998
Author(s):  
Beibei Qi ◽  
Chao Wu ◽  
Huiling Liang ◽  
Kehui Cui ◽  
Shah Fahad ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Acetic Acid ◽  
Abscisic Acid ◽  
Liquid Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Xylem Sap ◽  
Column Temperature ◽  
Relative Standard ◽  
Indole 3 Acetic Acid

Liquid-liquid extraction and solid phase extraction followed by high-performance liquid chromatography (HPLC) connected with ultraviolet (UV) detection were used for the determination of phytohormones. The parameters influencing the performance of the HPLC-UV method, including composition of the mobile phase for gradient elution, column temperature, flow rate, and detection wavelength, were optimized. This method can simultaneously determine 11 phytohormones, including nine cytokinins, indole-3-acetic acid, and abscisic acid. The limit of detection of this method is 0.22 to 1.1 µg L−1, and the coefficient factors of linear regression are >0.998. The recoveries of the target phytohormones ranged between 62.1~109.4%, and the relative standard deviations were <10%. This method is suitable for determining phytohormones, especially cytokinins, in young panicles, roots, and xylem sap of rice plants.

scholarly journals Determination of Lipid Oxidation Level in Broiler Meat by Liquid Chromatography

2004 ◽  
Vol 87 (5) ◽  
pp. 1148-1152 ◽  
Author(s):  
Slavomír Marcinčák ◽  
Jozef Sokol ◽  
Pavel Bystrický ◽  
Peter Popelka ◽  
Peter Turek ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
Trichloroacetic Acid ◽  
Solid Phase ◽  
Extraction Methods ◽  
Reversed Phase ◽  
Phase Extraction ◽  
Broiler Meat

Abstract An assay was conducted for the determination of malondialdehyde (MDA) levels in broiler meat. The method involves extraction of tissues with trichloroacetic acid (TCA) and reaction of the TCA extract with 2,4-dinitrophenylhydrazine (DNPH). After separation of the MDA-DNPH complex using a solid-phase extraction C18 column, samples were eluted with 1 mL acetonitrile. Aliquots of 20 μL acetonitrile were analyzed by liquid chromatography on reversed-phase C18 column (3 μm) with UV detection. The products were eluted isocratically with the mobile phase containing acetonitrile–water–acetic acid (39 + 61 + 0.2, v/v/v). The retention time was for MDA-DNPH was 6.5 min, and the detection limit was 3.5 μg/kg. Two extraction methods (cold and hot) were also used in the study. The results showed that hot extraction increased results about 55.8% and recovery from samples spiked with 116.6 μg/kg was lower (74.6%) in comparison with cold extraction (94.7%).

Sign in / Sign up

Export Citation Format

Share Document