scholarly journals Production of β-Glucosidase from a Newly IsolatedAspergillusSpecies Using Response Surface Methodology

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Pilanee Vaithanomsat ◽  
Molnapat Songpim ◽  
Taweesiri Malapant ◽  
Akihiko Kosugi ◽  
Warunee Thanapase ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ammonium Sulfate ◽  
Yeast Extract ◽  
Culture Medium ◽  
Specific Activity ◽  
Ph Value ◽  
Sulfate Concentration ◽  
Extract Concentration ◽  
Fermentation Parameters

A newly isolated fungusAspergillus nigerSOI017 was shown to be a good producer of β-glucosidase from all isolated fungal strains. Fermentation condition (pH, cellobiose concentration, yeast extract concentration, and ammonium sulfate concentration) was optimized for producing the enzyme in shake flask cultures. Response surface methodology was used to investigate the effects of 4 fermentation parameters (yeast extract concentration, cellobiose concentration, ammonium sulfate concentration, and pH) on β-glucosidase enzyme production. Production of β-glucosidase was most sensitive to the culture medium, especially the nitrogen source yeast extract. The optimized medium for producing maximum β-glucosidase specific activity consisted of 0.275% yeast extract, 1.125% cellobiose, and 2.6% ammonium sulfate at a pH value of 3.

scholarly journals Design and optimization of hydrogen production from hydrothermally pretreated sugarcane bagasse using response surface methodology

2017 ◽  
Vol 76 (1) ◽  
pp. 95-105 ◽  
Author(s):  
Lais Américo Soares ◽  
Juliana Kawanishi Braga ◽  
Fabrício Motteran ◽  
Isabel Kimiko Sakamoto ◽  
Edson Luiz Silva ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Hydrogen Production ◽  
Response Surface ◽  
Sugarcane Bagasse ◽  
Yeast Extract ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Incubation Temperature ◽  
P Value ◽  
Design And Optimization ◽  
Extract Concentration

Hydrogen production from hydrothermally pretreated (200 °C for 10 min at 16 bar) sugarcane bagasse was analyzed using response surface methodology. The yeast extract concentration and the temperature had a significant influence for hydrogen production (p-value 0.027 and 0.009, respectively). Maximum hydrogen production (17.7 mmol/L) was observed with 3 g/L yeast extract at 60 °C (C10). In this conditions were produced acetic acid (50.44 mg/L), butyric acid (209.71 mg/L), ethanol (38.4 mg/L), and methane (6.27 mmol/L). Lower hydrogen productions (3.5 mmol/L and 3.9 mmol/L) were observed under the conditions C7 (2 g/L of yeast extract, 35.8 °C) and C9 (1 g/L of yeast extract, 40 °C), respectively. The low yeast extract concentration and low temperature caused a negative effect on the hydrogen production. By means of denaturing gradient gel electrophoresis 20% of similarity was observed between the archaeal population of mesophilic (35 and 40 °C) and thermophilic (50, 60 and 64 °C) reactors. Likewise, similarity of 22% was noted between the bacterial population for the reactors with the lowest hydrogen production (3.5 mmol/L), at 35.8 °C and with the highest hydrogen production (17.7 mmol/L) at 60 °C demonstrating that microbial population modification was a function of incubation temperature variation.

scholarly journals Statistical optimization of culture medium for yellow pigment production by Thermomyces sp.

2015 ◽  
Vol 7 (1) ◽  
pp. 203-210
Author(s):  
R. Poorniammal ◽  
S. Gunasekaran ◽  
R. Murugesan
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Yeast Extract ◽  
Culture Medium ◽  
Yellow Pigment ◽  
Pigment Production ◽  
Quadratic Model ◽  
High Yield ◽  
Dipotassium Hydrogen Phosphate ◽  
Yellow Pigments

In present study, Thermomyces sp. were able to produce high yield of yellow pigments screened. Pigment production by Thermomyces sp was optimized by employing factorial design and response surface techniques in submerged fermentation. The variables evaluated were the concentrations of, sucrose, yeast extract, ammonium sulphate, magnesium sulphate and dipotassium hydrogen phosphate having as response pigment production. One factor at-a-time method was employed for the optimization of media components. Response surface methodology (RSM) optimized these nutrient parameters for maximum yellow pigment production (1387 OD units), which resulted at 35.5 g/L sucrose 5.5 g/L yeast extract, 2.5 g/L NH4SO4, 0.3 g/L MgSO4 and 1.0 g/L K2HPO4 in the medium. Response surface methodology (RSM) was further used to determine the optimum values of process variables for maximum yellow pigment production. The fit of the quadratic model was found to be significant. A significant increase in yellow pigment production was achieved using RSM.

Optimisation of medium composition for probiotic biomass production using response surface methodology

2013 ◽  
Vol 81 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Masumeh Anvari ◽  
Gholam Khayati ◽  
Shora Rostami
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Yeast Extract ◽  
Polynomial Regression ◽  
Probiotic Bacterium ◽  
Medium Composition ◽  
Bifidobacterium Animalis ◽  
Extract Concentration ◽  
Initial Ph ◽  
Probiotic Biomass

This study was aimed to optimise lactose, inulin and yeast extract concentration and also culture pH for maximising the growth of a probiotic bacterium,Bifidobacterium animalissubsp.lactisin apple juice and to assess the effects of these factors by using response surface methodology. A second-order central composite design was applied to evaluate the effects of these independent variables on growth of the microorganism. A polynomial regression model with cubic and quadratic terms was used for analysis of the experimental data. It was found that the effects involving inulin, yeast extract and pH on growth of the bacterium were significant, and the strongest effect was given by the yeast extract concentration. Estimated optimum conditions of the factors on the bacterial growth are as follows: lactose concentration=9·5 g/l; inulin concentration=38·5 mg/l; yeast extract concentration=9·6 g/l and initial pH=6·2.

scholarly journals Production, characterization and application of inulinase from fungal endophyte CCMB 328

2012 ◽  
Vol 84 (2) ◽  
pp. 443-454 ◽  
Author(s):  
Diego S. Nascimento ◽  
Gildomar Valasques Junior ◽  
Pedro Fernandes ◽  
Geise C.A. Ribeiro ◽  
Danyo M. Lima ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Yeast Extract ◽  
Arid Region ◽  
Culture Medium ◽  
Fungal Endophyte ◽  
Initial Concentration ◽  
Inulin Hydrolysis ◽  
Semi Arid Region ◽  
Hydrolysis Of ◽  
Semi Arid

Inulinase (β-2,1-D- fructan fructanohydrolase), EC 3.2.1.7, targets the β-2,1 linkage of inulin, a polyfructan consisting of linear β-2,1 linked fructose, and hydrolyzes it into fructose. This use provides an alternative to produce fructose syrup through the hydrolysis of inulin. The objective of this work was to study the production, characterization and applications of inulinases from the fungal endophyte CCMB 328 isolated from the Brazilian semi-arid region. Response Surface Methodology (RSM) was employed to evaluate the effect of variables (concentration of glucose and yeast extract), on secreted inulinase activities detected in the culture medium and also in the inulin hydrolysis. The results showed that the best conditions for inulinase production by CCMB 328 are 9.89 g / L for glucose and 1.09 g / L for yeast extract. The concentration of 0.20 mol/L of NaCl and KCl increased the activity of inulinase from CCMB 328 by approximately 63% and 37%, respectively. The results also showed that the inulinase has potential for inulin hydrolysis, whose conversion yields roughly 72.48 % for an initial concentration of inulin at 1% (w/v).

scholarly journals Application of electrochemical peroxidation (ECP) process for waste-activated sludge stabilization and system optimization using response surface methodology (RSM)

2018 ◽  
Vol 77 (6) ◽  
pp. 1765-1776 ◽  
Author(s):  
Gagik Badalians Gholikandi ◽  
Khashayar Kazemirad
Keyword(s):  
Response Surface Methodology ◽  
Activated Sludge ◽  
Response Surface ◽  
Removal Efficiency ◽  
Ph Value ◽  
System Optimization ◽  
Waste Activated Sludge ◽  
Operational Conditions ◽  
Sludge Stabilization ◽  
Rsm Optimization

Abstract In this study, the performance of the electrochemical peroxidation (ECP) process for removing the volatile suspended solids (VSS) content of waste-activated sludge was evaluated. The Fe2+ ions required by the process were obtained directly from iron electrodes in the system. The performance of the ECP process was investigated in various operational conditions employing a laboratory-scale pilot setup and optimized by response surface methodology (RSM). According to the results, the ECP process showed its best performance when the pH value, current density, H2O2 concentration and the retention time were 3, 3.2 mA/cm2, 1,535 mg/L and 240 min, respectively. In these conditions, the introduced Fe2+ concentration was approximately 500 (mg/L) and the VSS removal efficiency about 74%. Moreover, the results of the microbial characteristics of the raw and the stabilized sludge demonstrated that the ECP process is able to remove close to 99.9% of the coliforms in the raw sludge during the stabilization process. The energy consumption evaluation showed that the required energy of the ECP reactor (about 1.8–2.5 kWh (kg VSS removed)−1) is considerably lower than for aerobic digestion, the conventional waste-activated sludge stabilization method (about 2–3 kWh (kg VSS removed)−1). The RSM optimization process showed that the best operational conditions of the ECP process comply with the experimental results, and the actual and the predicted results are in good conformity with each other. This feature makes it possible to predict the introduced Fe2+ concentrations into the system and the VSS removal efficiency of the process precisely.

Bioremediation Condition Optimization of Hydrocarbon in Soil Using Response Surface Methodology by Microbial Consortium KL9-1

2012 ◽  
Vol 518-523 ◽  
pp. 2073-2078 ◽  
Author(s):  
Qi You Liu ◽  
Yun Bo Zhang ◽  
Dong Feng Zhao ◽  
Chao Cheng Zhao
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Microbial Consortium ◽  
Ph Value ◽  
Removal Rate ◽  
Desirability Function ◽  
Nitrogen And Phosphorus ◽  
Predictive Values ◽  
Independent Variables ◽  
Condition Optimization

A response surface methodology was applied to optimize the bioremediation condition of hydrocarbon in soil by microbial consortium KL9-1. A four-level Box-Behnken factorial design was employed to study the relationship of independent variables and dependent variable by applying pH value, inoculation amount of microbial consortium KL9-1, ratio of nitrogen and phosphorus (N/P ) and surfactant (SDBS) concentration as independent variables (factors) and crude oil removal rate as dependent variable (response). Then the statistically significant model was obtained and numerical optimization based on desirability function was carried out for pH 7.0, inoculation amount 50.0 mL, N/P 2: 1 and SDBS concentration 4.0 g, and the hydrocarbon removal rate reached as high as 52.58%. The predictive values showed good agreement with the experimental values under the optimization conditions, by standard variance <1.3%. It showed that the optimal result was reliable.

Evaluation of heterogeneous photo-Fenton oxidation of Orange II using response surface methodology

2010 ◽  
Vol 62 (6) ◽  
pp. 1320-1326 ◽  
Author(s):  
Y. H. Gong ◽  
H. Zhang ◽  
Y. L. Li ◽  
L. J. Xiang ◽  
S. Royer ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Heterogeneous Catalyst ◽  
Uv Irradiation ◽  
Heterogeneous Catalysts ◽  
Ph Value ◽  
Dye Degradation ◽  
High Catalytic Activity ◽  
Orange Ii ◽  
Initial Ph

A mesoporous SBA-15 doped iron oxide (Fe2O3/SBA-15) was synthesized by co-codensation, characterized and used as heterogeneous catalysts for the photo-Fenton decolorization of azo dye Orange II under UV irradiation. Response surface methodology (RSM) was used to investigate operating condition effects, such as hydrogen peroxide concentration, initial pH and catalyst loadings, on the decolorization rate. UV irradiation is found to enhance the activity of the catalyst in the process. RSM analysis evidenced the influence of the initial pH value and H2O2 concentration on the dye degradation rate. The coupled UV/Fe2O3/SBA-15/H2O2 process at room temperature is revealed as a promising friendly process for wastewater treatment. Indeed, the use of a heterogeneous catalyst allows an easy active phase recycling without multi-step recovering while the heterogeneous catalyst used here exhibits high catalytic activity for the reaction considered.

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