scholarly journals Electroacupuncture-Induced Cholinergic Nerve Activation Enhances the Hypoglycemic Effect of Exogenous Insulin in a Rat Model of Streptozotocin-Induced Diabetes

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Yu-Chen Lee ◽  
Te-Mao Li ◽  
Chung-Yuh Tzeng ◽  
Yu-Wen Cheng ◽  
Ying-I Chen ◽  
...  
Keyword(s):  
Insulin Signaling ◽  
Challenge Test ◽  
Diabetic Rats ◽  
Hypoglycemic Effect ◽  
Control Group ◽  
Exogenous Insulin ◽  
Glucose Lowering ◽  
Cholinergic Nerve ◽  
Streptozotocin Induced Diabetes ◽  
The Relationship

The aim of this study is to explore the mechanisms by which electroacupuncture (EA) enhances the hypoglycemic effect of exogenous insulin in a streptozotocin- (STZ-) diabetic rats. Animals in the EA group were anesthetized and subjected to the insulin challenge test (ICT) and EA for 60 minutes. In the control group, rats were subjected to the same treatment with the exception of EA stimulation. Blood samples were drawn to measure changes in plasma glucose, free fatty acids (FFA), and insulin levels. Western blot was used to assay proteins involved in insulin signaling. Furthermore, atropine, hemicholinium-3 (HC-3), and Eserine were used to explore the relationship between EA and cholinergic nerve activation during ICT. EA augmented the blood glucose-lowering effects of EA by activating the cholinergic nerves in STZ rats that had been exposed to exogenous insulin. This phenomenon may be related to enhancement of insulin signaling rather than to changes in FFA concentration.

scholarly journals Hypoglycemic Potential of Dietary Supplementation of Protein Isolate from Fermented Cucumeropsis manii in Streptozotocin Induced Hyperglycemic in Male Wistar Albino Rats

2021 ◽  
pp. 31-42
Author(s):  
A. O. Abiola ◽  
A. O. Iyoribhe ◽  
S. A. Adeniyi ◽  
O. B. Adu ◽  
A. S. Ogunbowale ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Concentration ◽  
Diabetic Control ◽  
Protein Isolate ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Antioxidant Enzyme Activities ◽  
Cvd Risk ◽  
Streptozotocin Induced Diabetes

The effect of Protein isolate from fermented melon seeds (Ogiri Protei Isolates; OPI) of Cucumeropsis manii on blood glucose, lipid profile, and antioxidant enzyme activities in streptozotocin (STZ)-induced diabetic rats was investigated. Thirty Male Wistar rats were divided into five equal groups. GThe first control group with no exposure. The second group of rats with Streptozotocin-induced non-treated diabetes. The 3rd and 4th groups of rats with Streptozotocin-induced diabetes supplemented with Ogiri protein isolates (200, 600 mg/kg in diet). And the 5th group of rats with Streptozotocin-induced diabetes administered glibenclamide in a dose 500 ug/kg in diet [17]. The OPI was administered for 6 weeks. The administration of OPI reduced the blood glucose concentration of the STZ-induced diabetic rats. Sera and hepatic superoxide dismutase, activities of the STZ-induced diabetic rats were significantly (P< 0.05) increased in comparison with the diabetic control rats. Lipid peroxidation of the supplemented OPI diabetic rats was significantly (P< 0.05) decreased in comparison with the diabetic control rats as the administration of OPI to the STZ-induced diabetic rats significantly increased the enzymes’ activities. The concentration of low-density lipoproteins in the OPI supplemented rats was significantly elevated. These data demonstrate that OPI supplements might be beneficial for correcting hyperglycemia but the consumption of OPI can modulate some tissue lipids in a direction not beneficial for CVD risk in patients with diabetes.

Inhibitory Effect of Astraxanthin Combined with Flavangenol® on Oxidative Stress Biomarkers in Streptozotocin-Iduced Diabetic Rats

2008 ◽  
Vol 78 (45) ◽  
pp. 175-182 ◽  
Author(s):  
Masako Nakano ◽  
Natsumi Orimo ◽  
Nakako Katagiri ◽  
Masahito Tsubata ◽  
Jiro Takahashi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxide ◽  
Inhibitory Effect ◽  
Diabetic Rats ◽  
Control Group ◽  
Cataract Formation ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Liver And Kidney ◽  
Streptozotocin Induced Diabetes

In this study, the effect of dietary antioxidants, such as astaxanthin and Flavangenol®, and a combination of both, in counteracting oxidative stress in streptozotocin-induced diabetes was investigated. Streptozotocin-induced diabetic rats were divided into four groups: control, astaxanthin, Flavangenol, and combined astaxanthin and Flavangenol (mix group). Each group other than the control group was fed with an astaxanthin diet (0.1 g/kg), Flavangenol diet (2.0 g/kg), or an astaxanthin (0.1 g/kg)-Flavangenol (2.0 g/kg) mixture diet, respectively. After 12 weeks of feeding, the results showed that the lipid peroxide levels of plasma and lens and the plasma triglyceride (TG) level in the mix group were significantly decreased by 44%, 20%, and 20%, respectively, compared with the control group. In the mix group, lipid peroxidation was also significantly reduced by 70% in the liver and 20% in the kidney compared with the control group. Furthermore, the level of urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) in the mix group was significantly lower, 36%, than the control group. The α-tocopherol concentrations in the plasma, liver, and kidney in the astaxanthin and mix groups were significantly higher, 3-9 times, than in the control group. The degree of cataract formation in the Flavangenol and mix groups tended to be lower than the control group. These results indicate that the combination of astaxanthin with Flvangenol has an improved protective effect on oxidative stress associated with streptozotocin-induced diabetes than either agent used alone. Thus, this combination may be beneficial in preventing the progression of diabetic complications.

Change in tissue concentrations of lipid hydroperoxides, vitamin C and vitamin E in rats with streptozotocin-induced diabetes

1999 ◽  
Vol 96 (2) ◽  
pp. 185-190 ◽  
Author(s):  
Fang SUN ◽  
Kozue IWAGUCHI ◽  
Reiko SHUDO ◽  
Yoshie NAGAKI ◽  
Kyoko TANAKA ◽  
...  
Keyword(s):  
Vitamin E ◽  
Vitamin C ◽  
Tissue Concentration ◽  
Diabetic Rats ◽  
Control Group ◽  
Specific Method ◽  
Lipid Hydroperoxides ◽  
Tissue Concentrations ◽  
Streptozotocin Induced Diabetes ◽  
The Brain

The tissue concentration of lipid hydroperoxides, which was determined by a specific method involving chemical derivatization and HPLC, increased significantly in the heart, liver, kidney and muscle of diabetic rats 8 weeks after the intraperitoneal injection of streptozotocin compared with that of the control group. These results demonstrate that an enhanced oxidative stress is caused in these tissues by diabetes. Vitamin C concentrations of the brain, heart, lung, liver, kidney and plasma of the diabetic rats decreased significantly after 8 weeks compared with those of the control group. Vitamin E concentrations of the brain, heart, liver, kidney, muscle and plasma of the diabetic rats increased significantly after 4 weeks compared with the control group. After 8 weeks, an elevation in vitamin E concentration was observed in the heart, liver, muscle and plasma of the diabetic rats.

scholarly journals Modulation of Renal Insulin Signaling Pathway and Antioxidant Enzymes with Diabetes: Effects of Resveratrol

2018 ◽  
Author(s):  
Gökhan Sadi ◽  
Gamze Şahin ◽  
Aykut Bostancı
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Signaling Pathway ◽  
Insulin Signaling ◽  
Diabetic Rats ◽  
Insulin Signaling Pathway ◽  
Inflammatory Agent ◽  
Male Wistar Rats ◽  
The Relationship ◽  
Control State

Diabetes mellitus, a disease arising by the deficiency of insulin hormone or its inability of usage, affects carbohydrate, lipid and protein metabolism, and destruct variety of the tissues. A strong antioxidant and anti-inflammatory agent; resveratrol has a high potential to prevent or treat the pathogenesis of diseases. This study was conducted to reveal the relationship between diabetes-induced oxidative stress and tissue inflammation with changes in antioxidant enzymes (cat, sod, gpx, and gst) and the components of insulin signaling pathway (insulin R&beta;, irs-1, pi3k, akt, mtor) in kidney tissues. Additionally, the effects of resveratrol on these parameters were evaluated. Male Wistar rats were randomly divided into four groups; (1) control/vehicle; (2) control/20 mg/kg resveratrol; (3) diabetic/vehicle; (4) diabetic/20 mg/kg resveratrol. Results demonstrated down-regulation of antioxidant enzymes in the kidney tissues of diabetic rats and this situation was devoted partially to the reduced expression of nf&kappa;b. Moreover, the components of renal insulin signaling elements were up-regulated in diabetic rats, and resveratrol treatment decreased this sensitization towards the control state. In conclusion, resveratrol improved diabetes-induced renal oxidative stress and inflammation partly due to healing action on renal antioxidant enzymes and insulin signaling pathway components.

Reversibility of renal tubular dysfunction in streptozotocin-induced diabetes in the rat

1992 ◽  
Vol 70 (7) ◽  
pp. 977-982 ◽  
Author(s):  
S. Chouinard ◽  
C. Viau
Keyword(s):  
Molecular Weight ◽  
Urinary Excretion ◽  
Insulin Treatment ◽  
Diabetic Rats ◽  
Control Group ◽  
Low Molecular Weight ◽  
Tubular Dysfunction ◽  
Alanine Aminopeptidase ◽  
Group A ◽  
Streptozotocin Induced Diabetes

Enzymuria and specific proteinuria were examined over a period of 19 days in 4 groups of 5 rats: a control group, a non-diabetic polyuric group, a group of streptozotocin-induced diabetic rats treated with insulin as of the 10th day after the injection of the drug, and a similar group of untreated diabetic rats. Increased urinary excretion of β-N-acetyl-D-glucosaminidase, lactate dehydrogenase, and alanine aminopeptidase was observed shortly after the induction of diabetes. It was partly or totally reversible following insulin treatment. Nondiabetic polyuria had a slight effect on the excretion of alanine aminopeptidase only. The urinary excretion of β2-microglobulin also rapidly increased after the onset of diabetes to a level approximately 50 times the control values. This effect was largely reversible with insulin treatment and was absent in the nondiabetic polyuric group. A small but significant 3-fold increase in albumin excretion was also noted but was not affected by insulin treatment. We conclude that streptozotocin-induced diabetes causes an early tubular dysfunction that is unrelated to polyuria and is reversible upon insulin treatment. This tubular dysfunction is best revealed by the urinary excretion of the low molecular weight protein β2-microglobulin. Our results suggest that it would be of interest to further examine the usefulness of sensitive markers of tubular dysfunction, especially low molecular weight proteinuria, in the detection of early stages of diabetic nephropathy.Key words: diabetic nephropathy, enzyme, urine, proteinuria, β2-microglobulin, streptozotocin, insulin, rat.

scholarly journals Impact of Momordica Charantia (Karela) on the Proportion of Hepatocytes with Intranuclear Inclusions in the StreptozotocinInduced Diabetic Rats

2013 ◽  
Vol 5 (2) ◽  
pp. 84-86
Author(s):  
Shirin Mohal ◽  
Md. Afzal Hossain ◽  
Dulal Krishna Mondal ◽  
Shamim Ara ◽  
Khandaker Manzare Shamim
Keyword(s):  
Diabetic Rats ◽  
Momordica Charantia ◽  
Control Group ◽  
Intranuclear Inclusions ◽  
Significant Difference ◽  
Vehicle Injection ◽  
Streptozotocin Induced Diabetes ◽  
The Mean ◽  
Age Range ◽  
The Impact

Background: Momordica charantia has some hypoglycemic properties.Objective: The purpose of the present study was to find out the impact of Momordica charantia (karela) on the proportion of hepatocytes in the  Streptozotocin-induced  diabetic  rats.Methodology: This  was  an  animal  study  carried  out  in  the Department of Anatomy at Bangabandhu Sheikh Mujib Medical University (BSMMU) and Bangladesh Institute of Research & rehabilitation in Diabetes, Endocrine & metabolic Disorders (BIRDEM), Dhaka. Healthy young Long Evans rats of male sex weighing 150 to 280gm with an age range of 10 to 12 weeks were used in this study. The rats were divided into 4 equal groups depending upon their different sorts of dietary feedings and drug treatment. The variation of different proportion of hepatocytes with intranuclear inclusions in different groups of rat was monitored.Result: Sixty five rats were included in this study. Mean proportion of hepatocytes with intranuclear inclusions on day 51 from Streptozotocin/vehicle injection in the control group which was known as Group-A was 0. In untreated diabetic group the mean proportion of hepatocytes with intranuclear inclusions was 3.71 ± 0.82. On the other hand, in the insulin-treated diabetic rats the mean proportion of hepatocytes with intranuclear inclusions was 0 and in the karela-treated diabetic rats, the proportion of hepatocytes with intranuclear inclusions was 0. The value in the insulin-treated diabetic rats (p=0.0001) and in the karela-treated diabetic rats (p= 0.0001) were significantly lower than that of the untreated diabetic rats; however, there was no significant difference between the insulin-treated diabetic rats & the karela-treated diabetic rats (P>0.05) in this regard.Conclusion: Karela showed a tendency of acting against hyperglycemic effects of Streptozotocin-induced diabetes mellitus and also acting against the rise in proportion of hepatocytes with intranuclear inclusions in Streptozotocin-induced diabetes mellitus.DOI: http://dx.doi.org/10.3329/jssmc.v5i2.20761J Shaheed Suhrawardy Med Coll, 2013;5(2):84-86

scholarly journals Effect ofAgaricus blazeiMurill on the Pulmonary Tissue of Animals with Streptozotocin-Induced Diabetes

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
Fábio Cangeri Di Naso ◽  
Rodrigo Noronha de Mello ◽  
Sílvia Bona ◽  
Alexandre Simões Dias ◽  
Marilene Porawski ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Disease Onset ◽  
Treated Group ◽  
Diabetic Rats ◽  
Control Group ◽  
Agaricus Blazei ◽  
Pulmonary Tissue ◽  
Streptozotocin Induced Diabetes

The present study was designed to evaluate the oxidative stress as well as the therapeutic effect ofAgaricus blazeiMuril (A. Blazei) in rats with streptozotocin-induced diabetes. We used 25 Wistar rats, and DM was induced by injecting streptozotocin (70 mg/Kg i.p.).Agaricus blazeiMuril was administered daily starting 40 days after disease onset.A. Blazeiwas tested as an aqueous extract for its phytochemical composition, and its antioxidant activity in vitro was also evaluated. Lipoperoxidation (LPO), and superoxide dismutase (SOD), catalase, and glutathione peroxidase activities were measured in the pulmonary tissue, as well as the presence of inducible nitric oxide synthase (iNOS), through immunohistochemistry. An anatomopathologic study was also performed. Phytochemical screening ofA. Blazeidetected the presence of alkaloids and saponins. The extract exhibited a significant antioxidant activity in the DPPH-scavenging and the hipoxanthine/xanthine oxidase assays. Pulmonary LPO increased in diabetic animals (0.43±0.09;P<.001) as compared to the control group (0.18±0.02), followed by a reduction in theA. Blazei-treated group (0.33±0.04;P<.05). iNOS was found increased in the lung in diabetic rats and reduced in theA. Blazei-treated group. The pulmonary tissue in diabetic rats showed oxidative alterations related to the streptozotocin treatment. TheA. Blazeitreatment effectively reduced the oxidative stress and contributed to tissue recovery.

scholarly journals Lysosomal Exoglycosidase Profile and Secretory Function in the Salivary Glands of Rats with Streptozotocin-Induced Diabetes

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Mateusz Maciejczyk ◽  
Agnieszka Kossakowska ◽  
Julita Szulimowska ◽  
Anna Klimiuk ◽  
Małgorzata Knaś ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Inverse Correlation ◽  
Salivary Flow ◽  
Diabetic Rats ◽  
Secretory Function ◽  
Submandibular Glands ◽  
Streptozotocin Induced Diabetes ◽  
And Control ◽  
The Relationship

Before this study, there had been no research evaluating the relationship between a lysosomal exoglycosidase profile and secretory function in the salivary glands of rats with streptozotocin- (STZ-) induced type 1 diabetes. In our work, rats were divided into 4 groups of 8 animals each: control groups (C2, C4) and diabetic groups (STZ2, STZ4). The secretory function of salivary glands—nonstimulated and stimulated salivary flow,α-amylase, total protein—and salivary exoglycosidase activities—N-acetyl-β-hexosaminidase (HEX, HEX A, and HEX B),β-glucuronidase,α-fucosidase,β-galactosidase, andα-mannosidase—was estimated both in the parotid and submandibular glands of STZ-diabetic and control rats. The study has demonstrated that the activity of most salivary exoglycosidases is significantly higher in the parotid and submandibular glands of STZ-diabetic rats as compared to the healthy controls and that it increases as the disease progresses. Reduced secretory function of diabetic salivary glands was also observed. A significant inverse correlation between HEX B,α-amylase activity, and stimulated salivary flow in diabetic parotid gland has also been shown. Summarizing, STZ-induced diabetes leads to a change in the lysosomal exoglycosidase profile and reduced function of the salivary glands.

scholarly journals Morphological Changes of Gingiva in Streptozotocin Diabetic Rats

2009 ◽  
Vol 2009 ◽  
pp. 1-4 ◽  
Author(s):  
C. Tesseromatis ◽  
A. Kotsiou ◽  
H. Parara ◽  
E. Vairaktaris ◽  
M. Tsamouri
Keyword(s):  
Diabetes Mellitus ◽  
Morphological Changes ◽  
Diabetic Rats ◽  
Control Group ◽  
Diabetic Patients ◽  
Histological Investigation ◽  
Bacterial Diseases ◽  
Male Wistar Rats ◽  
Streptozotocin Induced Diabetes ◽  
Routine Assay

Gingivitis and periodontitis are chronic bacterial diseases of the underlying and surrounding tooth tissues. Diabetes mellitus is responsible for tooth deprivation both by decay and periodontal disease. The streptozotocin-induced diabetes results in a diabetic status in experimental animals similar to that observed in diabetes patients. The aim of the study was to investigate the relationship between the gingival lesions and the microangiopathy changes in streptozotocin-induced diabetes mellitus. Forty male Wistar rats were divided into two groups (control and experimental). Diabetes mellitus was induced by 45 mg/kg IV streptozotocin. The histological investigation of the marginal gingival and the relevant gingival papilla showed inflammation of the lamina propria and the squamous epithelium as well as marked thickness of the arteriole in the diabetic group, but no changes were observed in the control group. The results suggested a probable application of a routine gingival histological investigation in diabetic patients in order to control the progress of disease complications. It may be concluded that histological gingival investigation can be used as a routine assay for the control of the diabetic disease and prevention of its complications.

scholarly journals Chronic Unpredictable Mild Stress Aggravates Mood Disorder, Cognitive Impairment, and Brain Insulin Resistance in Diabetic Rat

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Hui Yang ◽  
Wei Li ◽  
Pan Meng ◽  
Zhuo Liu ◽  
Jian Liu ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Insulin Signaling ◽  
Diabetic Rats ◽  
Control Group ◽  
Mild Stress ◽  
Chronic Unpredictable Mild Stress ◽  
Diabetes Group ◽  
Brain Insulin ◽  
Immobility Time ◽  
Brain Insulin Resistance

Diabetes-induced brain insulin resistance is associated with many mental diseases, including depression. Epidemiological evidences demonstrate the pathophysiologic link between stress, depression, and diabetes. This study was designed to determine whether chronic unpredictable mild stress- (CUMS-) induced changes in brain insulin resistance could contribute to deterioration in mood and cognitive functions in diabetic rats. Male SD rats were randomly assigned to three groups, including standard control group, the diabetes group, and the diabetes with CUMS group. After 7 weeks, emotional behaviors and memory performances as well as metabolic phenotypes were measured. In addition, we examined the changes in protein expression related to brain insulin signaling. Our results show that rats in diabetes with CUMS group displayed a decreased locomotor behavior in open-field test, an increased immobility time in forced swim test, and tail suspension test, and an impaired learning and memory in the Morris water maze when compared to animals in diabetes group. Further, diabetes with CUMS exhibited a significant decrease in phosphorylation of insulin receptor and an increase phosphorylation of IRS-1 in brain. These results suggest that the depression-like behaviors and cognitive function impairments in diabetic rats with CUMS were related to the changes of brain insulin signaling.

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