scholarly journals The Application of Three-Dimensional Collagen-Scaffolds Seeded with Myoblasts to Repair Skeletal Muscle Defects

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Jianqun Ma ◽  
Kyle Holden ◽  
Jinhong Zhu ◽  
Haiying Pan ◽  
Yong Li
Keyword(s):  
Soft Tissue ◽  
Muscle Tissue ◽  
Tissue Repair ◽  
Composite Scaffold ◽  
Three Dimensional ◽  
Tissue Repair And Regeneration ◽  
Tissue Constructs ◽  
Defect Sites

Three-dimensional (3D) engineered tissue constructs are a novel and promising approach to tissue repair and regeneration. 3D tissue constructs have the ability to restore form and function to damaged soft tissue unlike previous methods, such as plastic surgery, which are able to restore only form, leaving the function of the soft tissue often compromised. In this study, we seeded murine myoblasts (C2C12) into a collagen composite scaffold and cultured the scaffold in a roller bottle cell culture system in order to create a 3D tissue graftin vitro. The 3D graft createdin vitrowas then utilized to investigate muscle tissue repairin vivo. The 3D muscle grafts were implanted into defect sites created in the skeletal muscles in mice. We detected that the scaffolds degraded slowly over time, and muscle healing was improved which was shown by an increased quantity of innervated and vascularized regenerated muscle fibers. Our results suggest that the collagen composite scaffold seeded with myoblasts can create a 3D muscle graftin vitrothat can be employed for defect muscle tissue repairin vivo.

scholarly journals Tissue-engineered Oral Mucosa

2012 ◽  
Vol 91 (7) ◽  
pp. 642-650 ◽  
Author(s):  
K. Moharamzadeh ◽  
H. Colley ◽  
C. Murdoch ◽  
V. Hearnden ◽  
W.L. Chai ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
Oral Mucosa ◽  
Dental Materials ◽  
Three Dimensional ◽  
Bacterial Invasion ◽  
Graft Material ◽  
Oral Diseases

Advances in tissue engineering have permitted the three-dimensional (3D) reconstruction of human oral mucosa for various in vivo and in vitro applications. Tissue-engineered oral mucosa have been further optimized in recent years for clinical applications as a suitable graft material for intra-oral and extra-oral repair and treatment of soft-tissue defects. Novel 3D in vitro models of oral diseases such as cancer, Candida, and bacterial invasion have been developed as alternatives to animal models for investigation of disease phenomena, their progression, and treatment, including evaluation of drug delivery systems. The introduction of 3D oral mucosal reconstructs has had a significant impact on the approaches to biocompatibility evaluation of dental materials and oral healthcare products as well as the study of implant-soft tissue interfaces. This review article discusses the recent advances in tissue engineering and applications of tissue-engineered human oral mucosa.

An Investigation of Nano-structured Polymers for Use as Bladder Tissue Replacement Constructs

2001 ◽  
Vol 711 ◽  
Author(s):  
Anil Thapa ◽  
Thomas J. Webster ◽  
Karen M. Haberstroh
Keyword(s):  
Bladder Wall ◽  
Three Dimensional ◽  
The Body ◽  
Matrix Proteins ◽  
Bladder Smooth Muscle ◽  
Bladder Tissue ◽  
Tissue Replacement ◽  
Tissue Constructs

ABSTRACTConventionally, studies investigating the design of synthetic bladder wall substitutes have involved polymers with micro-dimensional structures. Since the body is made up of nano-structured components (e.g., extracellular matrix proteins), our focus has been in the use of nano-structured polymers in order to design a three-dimensional synthetic bladder construct that mimics bladder tissue in vivo. In order to complete this task, we fabricated novel, nano-structured, biodegradable materials to serve as substrates for bladder tissue constructs and tested the cytocompatibility properties of these biomaterials in vitro. The results from our in vitro work to date have provided the first evidence that cellular responses (such as adhesion and proliferation) of bladder smooth muscle cells are enhanced as poly (lactic-co-glycolic acid) (PLGA) surface feature dimensions are reduced into the nanometer range.

scholarly journals Trauma induced tissue survival in vitro with a muscle-biomaterial based osteogenic organoid system: a proof of concept study

2019 ◽  
Author(s):  
Tao He ◽  
Jörg Hausdorf ◽  
Yan Chevalier ◽  
Roland Manfred Klar
Keyword(s):  
Skeletal Muscle ◽  
Bone Tissue ◽  
Muscle Tissue ◽  
Three Dimensional ◽  
Good Alternative ◽  
Clinical Environment ◽  
In Vitro Systems ◽  
Osteogenic Gene Expression

Abstract Background The translation from animal research into the clinical environment remains problematic, as animal systems do not adequately replicate the human in vivo environment. Bioreactors have emerged as a good alternative that can reproduce part of the human in vivo processes at an in vitro level. However, in vitro bone formation platforms primarily utilizes stem cells only, with tissue based in vitro systems remaining poorly investigated. As such, the present pilot study explored the tissue behavior and cell survival capability within a new in vitro skeletal muscle tissue-based biomaterial organoid bioreactor system to maximize future bone tissue engineering prospects. Results Three dimensional printed β-tricalcium phosphate/hydroxyapatite devices were either wrapped in a sheet of rat muscle tissue or first implanted in a heterotopic muscle pouch that was then excised and cultured in vitro for up to 30 days. Devices wrapped in muscle tissue showed cell death by day 15. Contrarily, devices in muscle pouches showed angiogenic and limited osteogenic gene expression tendencies with consistent TGF-ß 1 , COL4A1 , VEGF-A , RUNX-2 , and BMP-2 upregulation, respectively. Histologically, muscle tissue degradation and fibrin release was seen being absorbed by devices acting possibly as a support for new tissue formation in the bioceramic scaffold that supports progenitor stem cell osteogenic differentiation.Conclusions These results therefore demonstrate that the skeletal muscle pouch-based biomaterial culturing system can support tissue survival over a prolonged culture period and represents a novel organoid tissue model that with further adjustments could generate bone tissue for direct clinical transplantations.

scholarly journals Design and Validation of Equiaxial Mechanical Strain Platform, EQUicycler, for 3D Tissue Engineered Constructs

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Mostafa Elsaadany ◽  
Matthew Harris ◽  
Eda Yildirim-Ayan
Keyword(s):  
Cell Lines ◽  
Mechanical Loading ◽  
Mechanical Load ◽  
Mechanical Strain ◽  
Three Dimensional ◽  
Cost Effective ◽  
Tissue Constructs ◽  
Tissue Systems

It is crucial to replicate the micromechanical milieu of native tissues to achieve efficacious tissue engineering and regenerative therapy. In this study, we introduced an innovative loading platform, EQUicycler, that utilizes a simple, yet effective, and well-controlled mechanism to apply physiologically relevant homogenous mechanical equiaxial strain on three-dimensional cell-embedded tissue scaffolds. The design of EQUicycler ensured elimination of gripping effects through the use of biologically compatible silicone posts for direct transfer of the mechanical load to the scaffolds. Finite Element Modeling (FEM) was created to understand and to quantify how much applied global strain was transferred from the loading mechanism to the tissue constructs. In vitro studies were conducted on various cell lines associated with tissues exposed to equiaxial mechanical loading in their native environment. In vitro results demonstrated that EQUicycler was effective in maintaining and promoting the viability of different musculoskeletal cell lines and upregulating early differentiation of osteoprogenitor cells. By utilizing EQUicycler, collagen fibers of the constructs were actively remodeled. Residing cells within the collagen construct elongated and aligned with strain direction upon mechanical loading. EQUicycler can provide an efficient and cost-effective tool to conduct mechanistic studies for tissue engineered constructs designed for tissue systems under mechanical loading in vivo.

In vitro and in vivo evaluation of the effect of nano-sized collagen molecules and nicotinamide on mesenchymal stem cell differentiation

2016 ◽  
Vol 4 (22) ◽  
pp. 3892-3902 ◽  
Author(s):  
Chin-Tsu Ma ◽  
Yi-Jhen Wu ◽  
Han Hsiang Huang ◽  
Pei-Leun Kang ◽  
Kuan Yin Hsiao ◽  
...  
Keyword(s):  
Tissue Repair ◽  
Stem Cell Differentiation ◽  
In Vivo Evaluation ◽  
Cell Replacement ◽  
Tissue Repair And Regeneration ◽  
Mesenchymal Stem Cell Differentiation ◽  
Collagen Molecules ◽  
Stromal Stem Cells

Advances and improvements in mesenchymal stromal/stem cells (MSCs) and cell replacement therapies have been promising approaches to treat diabetes mellitus (DM) since their potent capacities for differentiation into various functional cells match the demands of tissue repair and regeneration.

scholarly journals Bio-Scaffolds as Cell or Exosome Carriers for Nerve Injury Repair

2021 ◽  
Vol 22 (24) ◽  
pp. 13347
Author(s):  
Raju Poongodi ◽  
Ying-Lun Chen ◽  
Tao-Hsiang Yang ◽  
Ya-Hsien Huang ◽  
Kuender D. Yang ◽  
...  
Keyword(s):  
Clinical Outcome ◽  
Nerve Injury ◽  
Three Dimensional ◽  
Clinical Results ◽  
Tissue Repair And Regeneration ◽  
Axonal Regrowth ◽  
Acid Protein ◽  
Implantation Techniques

Central and peripheral nerve injuries can lead to permanent paralysis and organ dysfunction. In recent years, many cell and exosome implantation techniques have been developed in an attempt to restore function after nerve injury with promising but generally unsatisfactory clinical results. Clinical outcome may be enhanced by bio-scaffolds specifically fabricated to provide the appropriate three-dimensional (3D) conduit, growth-permissive substrate, and trophic factor support required for cell survival and regeneration. In rodents, these scaffolds have been shown to promote axonal regrowth and restore limb motor function following experimental spinal cord or sciatic nerve injury. Combining the appropriate cell/exosome and scaffold type may thus achieve tissue repair and regeneration with safety and efficacy sufficient for routine clinical application. In this review, we describe the efficacies of bio-scaffolds composed of various natural polysaccharides (alginate, chitin, chitosan, and hyaluronic acid), protein polymers (gelatin, collagen, silk fibroin, fibrin, and keratin), and self-assembling peptides for repair of nerve injury. In addition, we review the capacities of these constructs for supporting in vitro cell-adhesion, mechano-transduction, proliferation, and differentiation as well as the in vivo properties critical for a successful clinical outcome, including controlled degradation and re-absorption. Finally, we describe recent advances in 3D bio-printing for nerve regeneration.

scholarly journals Three-Dimensionally-Printed Polyether-Ether-Ketone Implant with a Cross-Linked Structure and Acid-Etched Microporous Surface Promotes Integration with Soft Tissue

2019 ◽  
Vol 20 (15) ◽  
pp. 3811 ◽  
Author(s):  
Xiaoke Feng ◽  
Hao Yu ◽  
Huan Liu ◽  
Xiaonan Yu ◽  
Zhihong Feng ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Rabbit Model ◽  
Three Dimensional ◽  
Zealand White Rabbit ◽  
Adhesion Properties ◽  
Polyether Ether Ketone ◽  
Ether Ketone ◽  
Materials Used

Polyether-ether-ketone (peek) is one of the most common materials used for load-bearing orthopedic devices owing to its radiolucency and favorable mechanical properties. However, current smooth-surfaced peek implants can lead to fibrous capsule formation. To overcome this issue, here, peek specimens with well-defined internal cross-linked structures (macropore diameters of 1.0–2.0 mm) were fabricated using a three-dimensional (3D) printer, and an acid-etched microporous surface was achieved using injection-molding technology. The cell adhesion properties of smooth and microporous peek specimens was compared in vitro through a scanning electron microscope (SEM), and the soft tissue responses to the both microporous and cross-linked structure of different groups were determined in vivo using a New Zealand white rabbit model, and examined through histologic staining and separating test. The results showed that the acid-etched microporous surface promoted human skin fibroblasts (HSF) adherence, while internal cross-linked structure improved the ability of the peek specimen to form a mechanical combination with soft tissue, especially with the 1.5 mm porous specimen. The peek specimens with both the internal cross-linked structure and external acid-etched microporous surface could effectively promote the close integration of soft tissue and prevent formation of fibrous capsules, demonstrating the potential for clinical application in surgical repair.

scholarly journals Bioengineered Fascicle-Like Skeletal Muscle Tissue Constructs

2012 ◽  
Author(s):  
Devin Neal ◽  
Mahmut Selman Sakar ◽  
H. Harry Asada
Keyword(s):  
Skeletal Muscle ◽  
Cell Density ◽  
Muscle Cell ◽  
Muscle Tissue ◽  
Skeletal Muscle Tissue ◽  
Muscle Injuries ◽  
Tissue Constructs

Tissue engineered skeletal muscle constructs have and will continue to be valuable in treating, and testing various muscle injuries and diseases. However a significant drawback to numerous methods of producing 3D skeletal muscle constructs grown in vitro is that muscle cell density as a fraction of total volume or mass, is often significantly lower than muscle found in vivo. Therefore a method to increase muscle cell density within a construct is needed.

scholarly journals Trauma induced tissue survival in vitro with a muscle-biomaterial based osteogenic organoid system: a proof of concept study

2019 ◽  
Author(s):  
Tao He ◽  
Jörg Hausdorf ◽  
Yan Chevalier ◽  
Roland Manfred Klar
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Muscle Tissue ◽  
Three Dimensional ◽  
Good Alternative ◽  
Clinical Environment

Abstract Background: The translation from animal research into the clinical environment remains problematic, as animal systems do not adequately replicate the human in vivo environment. Bioreactors have emerged as a good alternative that can reproduce part of the human in vivo processes at an in vitro level. Bone tissue-engineering bioreactors, however, still are cell based with tissue based in vitro systems remaining poorly investigated. As such, the present pilot study explored the tissue behavior and cell survival capability within a new in vitro skeletal muscle tissue-based biomaterial organoid bioreactor system to maximize future bone tissue engineering prospects. Results: Three dimensional printed β-tricalcium phosphate/hydroxyapatite devices were either wrapped in a sheet of rat muscle tissue or first implanted in a heterotopic muscle pouch that was then excised and cultured in vitro for up to 30 days. Devices wrapped in muscle tissue showed cell death by day 15. Contrarily, devices in muscle pouches showed angiogenic and limited osteogenic gene expression tendencies with consistent TGF-ß1, COL4A1, VEGF-A, RUNX-2, and BMP-2 upregulation, respectively. Histologically, muscle tissue degradation and fibrin release was seen being absorbed by devices acting possibly as a support for new tissue formation in the bioceramic scaffold that supports progenitor stem cell osteogenic differentiation.Conclusions: These results therefore demonstrate that the skeletal muscle pouch-based biomaterial culturing system can support tissue survival over a prolonged culture period and represents a novel organoid tissue model that with further adjustments could generate bone tissue for direct clinical transplantations.

scholarly journals A facile, versatile hydrogel bioink for 3D bioprinting benefits long-term subaqueous fidelity, cell viability and proliferation

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
Hongqing Chen ◽  
Fei Fei ◽  
Xinda Li ◽  
Zhenguo Nie ◽  
Dezhi Zhou ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Cell Viability ◽  
Tissue Repair ◽  
Three Dimensional ◽  
Water Retention Capacity ◽  
3D Bioprinting ◽  
Soft Tissue Repair ◽  
Retention Capacity

Abstract Both of the long-term fidelity and cell viability of three-dimensional (3D)-bioprinted constructs are essential to precise soft tissue repair. However, the shrinking/swelling behavior of hydrogels brings about inadequate long-term fidelity of constructs, and bioinks containing excessive polymer are detrimental to cell viability. Here, we obtained a facile hydrogel by introducing 1% aldehyde hyaluronic acid (AHA) and 0.375% N-carboxymethyl chitosan (CMC), two polysaccharides with strong water absorption and water retention capacity, into classic gelatin (GEL, 5%)–alginate (ALG, 1%) ink. This GEL–ALG/CMC/AHA bioink possesses weak temperature dependence due to the Schiff base linkage of CMC/AHA and electrostatic interaction of CMC/ALG. We fabricated integrated constructs through traditional printing at room temperature and in vivo simulation printing at 37°C. The printed cell-laden constructs can maintain subaqueous fidelity for 30 days after being reinforced by 3% calcium chloride for only 20 s. Flow cytometry results showed that the cell viability was 91.38 ± 1.55% on day 29, and the cells in the proliferation plateau at this time still maintained their dynamic renewal with a DNA replication rate of 6.06 ± 1.24%. This work provides a convenient and practical bioink option for 3D bioprinting in precise soft tissue repair.

Sign in / Sign up

Export Citation Format

Share Document