scholarly journals Apoptotic Cell Death and Inhibition of Wnt/β-Catenin Signaling Pathway in Human Colon Cancer Cells by an Active Fraction (HS7) fromTaiwanofungus camphoratus

2011 ◽  
Vol 2011 ◽  
pp. 1-13 ◽  
Author(s):  
Chi-Tai Yeh ◽  
Chih-Jung Yao ◽  
Jiann-Long Yan ◽  
Shuang-En Chuang ◽  
Liang-Ming Lee ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Human Colon ◽  
Dependent Manner ◽  
Apoptosis Induction ◽  
Colon Cancer Cells ◽  
Active Fraction ◽  
Human Colon Cancer ◽  
Taiwanofungus Camphoratus ◽  
Human Colon Cancer Cells

Aberrant activation of Wnt/β-catenin signaling plays an important role in the development of colon cancer. HS7 is an active fraction extracted fromTaiwanofungus camphoratus, which had been widely used as complementary medicine for Taiwan cancer patients in the past decades. In this study, we demonstrated the effects of HS7 on the growth inhibition, apoptosis induction, and Wnt/β-catenin signaling suppression in human colon cancer cells. HS7 significantly inhibited proliferation of HT29, HCT116, and SW480 colon cancer cells in a dose- and time-dependent manner. The apoptosis induction was evidenced by DNA fragmentation and subG1 accumulation, which was associated with increased Bax/Bcl-2 ratio, activation of caspase-3 and cleavage of PARP. By using Tcf-dependent luciferase activity assay, HS7 was found to inhibit theβ-catenin/Tcf transcriptional activities. In addition, HS7 strongly suppressed the binding of Tcf complexes to its DNA-binding site shown in electrophoretic mobility shift assay. This inhibition was further confirmed by the decreased protein levels of Tcf-4 andβ-catenin. Theβ-catenin/Tcf downstream target genes, such assurvivin,c-myc,cyclin D1,MMP7, andMT1-MMPinvolved in apoptosis, invasion, and angiogenesis were also diminished as well. These results indicate thatTaiwanofungus camphoratusmay provide a benefit as integrative medicine for the treatment of colon cancer.

scholarly journals Chk1 and DNA-PK mediate TPEN-induced DNA damage in a ROS dependent manner in human colon cancer cells

2016 ◽  
Vol 17 (11) ◽  
pp. 1139-1148 ◽  
Author(s):  
Omar Nasser Rahal ◽  
Maamoun Fatfat ◽  
Carla Hankache ◽  
Bassam Osman ◽  
Hala Khalife ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Dna Damage ◽  
Cancer Cells ◽  
Human Colon ◽  
Dependent Manner ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Human Colon Cancer Cells

scholarly journals Zedoary Turmeric Oil Induces Senescence and Apoptosis in Human Colon Cancer HCT116 Cells

2018 ◽  
Vol 13 (7) ◽  
pp. 1934578X1801300
Author(s):  
Meng-Qi Su ◽  
Yi-Ran Zhou ◽  
Cheng-Qin Li ◽  
Zhou Wang ◽  
Yue-Liang Wang ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Western Blotting ◽  
Human Colon ◽  
Dependent Manner ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Related Proteins ◽  
Human Colon Cancer Cells ◽  
Hct116 Cells

Zedoary turmeric oil (ZTO) is a volatile oil that is extracted from the dry rhizome of Curcuma zedoaria with a variety of biological activities, including anti-tumor activity. However, there is a lack of knowledge about the effect and mechanism of ZTO in human colon cancer cells. The aim of this study was to examine the potential efficacy of ZTO against human colon cancer cells (HCT116) and to uncover the molecular mechanisms of its anti-tumor effects. The anti-proliferative activity of ZTO was determined by the MTT assay, cell counts and colony formation assay. Senescent cells were detected using SA-β-Gal staining, while apoptosis and the CD44+ subpopulation were evaluated by flow cytometry. The expression levels of senescence- and apoptosis-related proteins were examined using western blotting. The results showed that treatment with ZTO significantly inhibited the growth of HCT116 cells and caused senescence and apoptosis in a dose- and time-dependent manner. Western blotting revealed that ZTO significantly increased the expression of senescence- and apoptosis-related proteins p16, p21, and p53 and the phosphorylation of ERK. Moreover, ZTO treatment reduced the cancer stem-like CD44 positive cell population. These findings suggest that ZTO inhibits human colon cancer cells by inducing senescence and apoptosis.

Multidrug Resistance Reversal and Apoptosis Induction in Human Colon Cancer Cells by Some Flavonoids Present in Citrus Plants

2012 ◽  
Vol 75 (11) ◽  
pp. 1896-1902 ◽  
Author(s):  
Olga Wesołowska ◽  
Jerzy Wiśniewski ◽  
Kamila Środa-Pomianek ◽  
Aleksandra Bielawska-Pohl ◽  
Maria Paprocka ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Multidrug Resistance ◽  
Cancer Cells ◽  
Human Colon ◽  
Apoptosis Induction ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Resistance Reversal ◽  
Human Colon Cancer Cells

scholarly journals An in vitro investigation of photodynamic efficacy of FosPegⓇ on human colon cancer cells

2015 ◽  
Vol 08 (05) ◽  
pp. 1550027 ◽  
Author(s):  
Ricky W. K. Wu ◽  
Ellie S. M. Chu ◽  
Zheng Huang ◽  
Malini C. Olivo ◽  
David C. W. Ip ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Death ◽  
Cancer Cells ◽  
Human Colon ◽  
Light Dose ◽  
Dependent Manner ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Human Colon Cancer Cells

Photodynamic therapy (PDT) is a novel therapeutic approach for combating various cancers. PDT involves the administration of a photosensitizer which generates singlet oxygen after light activation. FosPegⓇ is the liposomal formulation of mTHPC. In this in vitro study, the photodynamic efficacy of FosPegⓇ on a human colon cancer cell line (HT29) was investigated via studying the cellular uptake of FosPegⓇ, FosPegⓇ PDT mediated photocytotoxicity and the cell death mechanism were triggered. FosPegⓇ PDT demonstrated its antitumor effect in a drug and light dose-dependent manner in HT-29 cells. Lethal dose (LD50) was achieved with 0.4 μg/mL of drug and 3 J/cm-2 of light dose. FosPegⓇ PDT triggered apoptotic cell death via activating caspase cascade and regulating cell cycle progression. In conclusion, FosPegⓇ-PDT is an effective measure to combat human colon cancer cells.

Leptin is a growth factor for human colon cancer cells

2001 ◽  
Vol 120 (5) ◽  
pp. A493-A493
Author(s):  
J HARDWICK ◽  
G VANDENBRINK ◽  
S VANDEVENTER ◽  
M PEPPELENBOSCH
Keyword(s):  
Colon Cancer ◽  
Growth Factor ◽  
Cancer Cells ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Human Colon Cancer Cells
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