scholarly journals Proteomic Analysis of Sera from Common Variable Immunodeficiency Patients Undergoing Replacement Intravenous Immunoglobulin Therapy

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Giuseppe Spadaro ◽  
Concetta D'Orio ◽  
Arturo Genovese ◽  
Antonella Galeotafiore ◽  
Chiara D'Ambrosio ◽  
...  
Keyword(s):  
Common Variable Immunodeficiency ◽  
Serum Proteins ◽  
Immunoglobulin Therapy ◽  
Normal Subjects ◽  
Serum Levels ◽  
Serum Samples ◽  
Intravenous Immunoglobulin Therapy ◽  
Proteomic Approach ◽  
Adults And Children ◽  
Common Variable

Common variable immunodeficiency is the most common form of symptomatic primary antibody failure in adults and children. Replacement immunoglobulin is the standard treatment of these patients. By using a differential proteomic approach based on 2D-DIGE, we examined serum samples from normal donors and from matched, naive, and immunoglobulin-treated patients. The results highlighted regulated expression of serum proteins in naive patients. Among the identified proteins, clusterin/ApoJ serum levels were lower in naive patients, compared to normal subjects. This finding was validated in a wider collection of samples from newly enrolled patients. The establishment of a cellular system, based on a human hepatocyte cell line HuH7, allowed to ascertain a potential role in the regulation ofCLUgene expression by immunoglobulins.

scholarly journals Major approaches in early diagnostics of common variable immunodeficiency in adults in Moscow

F1000Research ◽  
2012 ◽  
Vol 1 ◽  
pp. 46 ◽  
Author(s):  
Alexander V Karaulov ◽  
Irina V Sidorenko ◽  
Anna S Kapustina
Keyword(s):  
Common Variable Immunodeficiency ◽  
Structural Damage ◽  
Clinical Signs ◽  
Clinical Manifestations ◽  
Immunoglobulin Therapy ◽  
Early Diagnostics ◽  
Intravenous Immunoglobulin Therapy ◽  
Early Start ◽  
Treatment Concepts ◽  
Common Variable

Common variable immunodeficiency (CVID) is a primary immunological disease characterized predominantly by hypogammaglobulinemia. The main clinical manifestations are severe recurrent infections that often lead to structural damage of affected organs. The early start of adequate intravenous immunoglobulin therapy has significantly improved the prognosis of this serious disorder. Patients with CVID are also predisposed to autoimmune and lymphoproliferative complications. This article deals with the features of this primary immunodeficiency in adults. Clinical manifestations, immunological features and treatment concepts were gathered during 21 years of observation of such patients in Moscow. The authors suggest early predictive clinical signs of CVID in adults.

scholarly journals Differently expressed microRNA in response to the first Ig replacement therapy in common variable immunodeficiency patients

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Bruna De Felice ◽  
Ersilia Nigro ◽  
Rita Polito ◽  
Francesca Wanda Rossi ◽  
Antonio Pecoraro ◽  
...  
Keyword(s):  
Replacement Therapy ◽  
Common Variable Immunodeficiency ◽  
Antigen Processing ◽  
Serum Samples ◽  
Primary Immunodeficiency Disorder ◽  
Class I Mhc ◽  
Treatment Naïve ◽  
Delays In Diagnosis ◽  
First Time ◽  
Common Variable

AbstractCommon variable immunodeficiency (CVID) is a complex primary immunodeficiency disorder characterized by a high clinical and genetic heterogeneity. The molecular underlying causes of CVID are not still now clear and the delays in diagnosis and treatment worsen the prognosis of the patients. MicroRNAs are non-coding, endogenous small RNAs often deregulated in human diseases, such as autoimmune and other immune-based disorders. In the present study, we aimed to evaluate miRNAs associated with the CVID and, in particular, with the response to the first Ig replacement therapy. To this aim, we compared miRNA profile obtained by serum samples of treatment-naïve CVID patients before and 24 h after the first Ig replacement therapy. For the first time, using a microarray assay followed by an integrated bioinformatics/biostatistics analysis, we identified five microRNAs (hsa-miR-6742, hsa-miR-1825, hsa-miR-4769-3p, hsa-miR-1228-3p, hsa-miR-1972) differently modulated in CVID patients by Ig infusion. All of them were down-regulated, excepted miR-6742 which was up-regulated. The latter may be of particular interest, since its functions are related to pathways involving Class I MHC mediated antigen processing and adaptive as well as innate Immune System. In conclusion, this study shows for the first time the modulation of miRNAs involved in CVID patients after the first Ig replacement therapy. Further studies are needed to assess whether such miRNAs could represent novel potential biomarkers in management and therapy of CVID patients.

Increased Serum Levels of Soluble CD30 in Patients with Common Variable Immunodeficiency and Its Clinical Implications

2007 ◽  
Vol 28 (1) ◽  
pp. 78-84 ◽  
Author(s):  
Nima Rezaei ◽  
Mostafa Haji-Molla-Hoseini ◽  
Asghar Aghamohammadi ◽  
Ali Akbar Pourfathollah ◽  
Mina Moghtadaie ◽  
...  
Keyword(s):  
Common Variable Immunodeficiency ◽  
Serum Levels ◽  
Clinical Implications ◽  
Soluble Cd30 ◽  
Common Variable
Sign in / Sign up

Export Citation Format

Share Document