scholarly journals Antioxidant and Antinociceptive Effects ofCitrus limonEssential Oil in Mice

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Lidianne Mayra Lopes Campêlo ◽  
Antonia Amanda C. de Almeida ◽  
Rizângela L. Mendes de Freitas ◽  
Gilberto Santos Cerqueira ◽  
Geane Felix de Sousa ◽  
...  
Keyword(s):  
Essential Oil ◽  
Antioxidant Potential ◽  
Opioid System ◽  
Citrus Limon ◽  
Antinociceptive Action ◽  
Action Mechanisms ◽  
Antinociceptive Effects ◽  
Scavenger Activity

The antioxidant and antinociceptive activities ofCitrus limonessential oil (EO) were assessed in mice orin vitrotests. EO possesses a strong antioxidant potential according to the scavenging assays. Moreover, it presented scavenger activity against allin vitrotests. Orally, EO (50, 100, and 150 mg/kg) significantly reduced the number of writhes, and, at highest doses, it reduced the number of paw licks. Whereas naloxone antagonized the antinociceptive action of EO (highest doses), this suggested, at least, the participation of the opioid system. Further studies currently in progress will enable us to understand the action mechanisms of EO.

scholarly journals Anxiolytic-like effect of Citrus limon (L.) Burm f. essential oil inhalation on mice

2016 ◽  
Vol 18 (1) ◽  
pp. 96-104 ◽  
Author(s):  
M.D.M. VIANA ◽  
R.M. CARDOSO ◽  
N.K.G.T. SILVA ◽  
M.A.P. FALCÃO ◽  
A.C.S. VIEIRA ◽  
...  
Keyword(s):  
Essential Oil ◽  
Intraperitoneal Administration ◽  
Anxiolytic Activity ◽  
Control Group ◽  
Citrus Limon ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Animal Models Of Anxiety

ABSTRACT Experimental in vivo study aimed to characterize the anxiolytic-like effect of the Citrus limon fruit peel’s essential oil (CLEO) in animal models of anxiety, besides evaluating the viability J774.A1 cells in vitro through the MTT reduction method at the concentrations of 10 and 100 µg/mL. The anxiolytic behavior was evaluated in Swiss mice (n = 8) using the methodology of Elevated Plus-Maze (EPM) and Open-Field (OF). CLEO was tested by inhalation at the doses of 100, 200, and 400 µL, and as control, animals were subjected to inhalation of the vehicle (saline solution 0.9% + Tween80®) and intraperitoneal administration of diazepam (1.5 mg/kg). In the cell viability assay, it was observed that none of the concentrations showed cytotoxicity. OF test showed significant anxiolytic activity at all tested doses of OECL, compared to the control group, without changing the motor performance of the animals. Corroborating OF data, the EPM test confirmed anxiolytic activity in at least two doses of the tested oil (200 and 400 µL), justified by the number of entries and increase in the percentage of time in the open arms. The data analysis of this study evidenced that inhalation of OECL was able to induce an anxiolytic behavior in mice; however, further studies are required to ensure its safe use by the population.

scholarly journals Chemoinformatics Approach to Antibacterial Studies of Essential Oils

2015 ◽  
Vol 10 (6) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Dragoljub L. Miladinović ◽  
Budimir S. Ilić ◽  
Branislava D. Kocić
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Antibacterial Activities ◽  
Bacterial Strains ◽  
Antibacterial Studies ◽  
Action Mechanisms ◽  
In Vitro Antibacterial Activity ◽  
Checkerboard Assay ◽  
Antagonistic Interactions

The chemical composition and antibacterial activity of Nepeta nuda (Lamiaceae) essential oil were examined, as well as the association between it and standard antibiotics: tetracycline and streptomycin. The antibacterial activities of 1,8-cineole, the main constituent of N. nuda oil, individually and in combination with standard antibiotics were also determined. The interactions of the essential oil and 1,8-cineole with antibiotics toward five selected strains were evaluated using the microdilution checkerboard assay in combination with chemoinformatics methods. Oxygenated monoterpenes were the most abundant compound class in the oil (57.8%), with 1,8-cineole (46.0%) as the major compound. The essential oil exhibited in vitro antibacterial activity against all tested bacterial strains, but the activities were lower than those of the standard antibiotics. The combinations N. nuda oil-antibiotic and 1,8-cineole-antibiotic produced a predominantly antagonistic interactions. Chemoinformatics survey confirms the antagonistic interactions as a consequence of membrane potential/proton motive force dissipation. These data indicate cytochrome c oxidase as a target for 1.8-cineole toxicity action mechanisms.

scholarly journals In Vitro and In Vivo Anti-Candida spp. Activity of Plant-Derived Products

Plants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 494 ◽  
Author(s):  
Reginaldo dos Santos Pedroso ◽  
Brenda Lorena Balbino ◽  
Géssica Andrade ◽  
Maria Cecilia Pereira Sacardo Dias ◽  
Tavane Aparecida Alvarenga ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Gallic Acid ◽  
Antifungal Drugs ◽  
Citrus Limon ◽  
Cupressus Sempervirens ◽  
Candida Spp ◽  
Fungistatic Activity

Candidiasis therapy, especially for candidiasis caused by Candida non-albicans species, is limited by the relatively reduced number of antifungal drugs and the emergence of antifungal tolerance. This study evaluates the anticandidal activity of 41 plant-derived products against Candida species, in both planktonic and biofilm cells. This study also evaluates the toxicity and the therapeutic action of the most active compounds by using the Caenorhabditis elegans–Candida model. The planktonic cells were cultured with various concentrations of the tested agents. The Cupressus sempervirens, Citrus limon, and Litsea cubeba essential oils as well as gallic acid were the most active anticandidal compounds. Candida cell re-growth after treatment with these agents for 48 h demonstrated that the L. cubeba essential oil and gallic acid displayed fungistatic activity, whereas the C. limon and C. sempervirens essential oils exhibited fungicidal activity. The C. sempervirens essential oil was not toxic and increased the survival of C. elegans worms infected with C. glabrata or C. orthopsilosis. All the plant-derived products assayed at 250 µg/mL affected C. krusei biofilms. The tested plant-derived products proved to be potential therapeutic agents against Candida, especially Candida non-albicans species, and should be considered when developing new anticandidal agents.

scholarly journals Antioxidant potential of essential oil from Lavandula angustifolia in in vitro and ex vivo cultured liver cells

Neoplasma ◽  
2017 ◽  
Vol 64 (04) ◽  
pp. 485-493 ◽  
Author(s):  
K. Kozics ◽  
A. Srancikova ◽  
E. Sedlackova ◽  
E. Horvathova ◽  
M. Melusova ◽  
...  
Keyword(s):  
Essential Oil ◽  
Ex Vivo ◽  
Liver Cells ◽  
Antioxidant Potential ◽  
Lavandula Angustifolia ◽  
Cultured Liver Cells

Chemical Composition and Antioxidant Potential of Essential Oil and Organic Extracts of Euphorbia tithymaloides L. from Kushtia Region

2019 ◽  
Vol 18 (10) ◽  
pp. 1482-1488 ◽  
Author(s):  
Atiqur Rahman ◽  
Mahmudur Rahman ◽  
Ibrahim Demirtas
Keyword(s):  
Essential Oil ◽  
Methanol Extract ◽  
Skin Diseases ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Antioxidant Potential ◽  
Traditional System ◽  
Nitric Oxide Radical ◽  
Organic Extracts

Background: In the traditional system of medicine, leaves and stem bark of Euphorbia tithymaloides L. have been used for the treatment of asthma, persistent coughing, laryngitis, skin diseases and mouth ulcers. Some studies have reported the anti-inflammatory and antimicrobial activities of phytochemicals from the leaf; however, the analysis of essential oil and its antioxidant property is still unexplored. Methods: This study evaluates the in vitro antioxidant potential of the essential oil and organic extracts from aerial parts of Euphorbia tithymaloides L. Results: Thirty one compounds representing 96.37% of total oil were detected by GC-MS, of which eugenol (22.52%), phenyl ethyl alcohol (14.63%), 3-pentanol (9.22%), caryophyllene oxide (7.73%), isoeugenol (7.32%), pentadecanol (5.14%), spathulenol (5.11%) and α-pinene (3.32%) were the major compounds. The oil and ethyl acetate extract displayed potent DPPH (IC50 = 13.67 and 17.59 µg/mL, respectively) and superoxide (IC50 = 21.83 and 42.34 µg/mL, respectively) radical-scavenging activities among all the tested samples. The oil and methanol extract also exhibited remarkable nitric oxide radical-scavenging activities (IC50 = 90.45 and 112.63 µg/mL, respectively) among other extracts. Furthermore, the methanol extract contained the highest amount of total phenolics as compared to other samples. Conclusion: The results demonstrate that the oil and extracts of E. tithymaloides could serve as natural antioxidants for using in pharmaceutical or cosmetic industries.

scholarly journals Comparison between Citral and Pompia Essential Oil Loaded in Phospholipid Vesicles for the Treatment of Skin and Mucosal Infections

Nanomaterials ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 286 ◽  
Author(s):  
Iris Usach ◽  
Elisabetta Margarucci ◽  
Maria Letizia Manca ◽  
Carla Caddeo ◽  
Matteo Aroffu ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Human Keratinocytes ◽  
Phospholipid Vesicles ◽  
Citrus Limon ◽  
Bacterial Strains ◽  
Physico Chemical ◽  
Health Promoting

Citrus species extracts are well known sources of bio-functional compounds with health-promoting effects. In particular, essential oils are known for their antibacterial activity due to the high content of terpenes. In this work, the steam-distilled essential oil from the leaves of Citrus limon var. pompia was loaded in phospholipid vesicles. The physico-chemical characteristics of the essential oil loaded vesicles were compared with those of vesicles that were loaded with citral, which is one of the most abundant terpenes of Citrus essential oils. The biocompatibility of the vesicles was assessed in vitro in human keratinocytes. Furthermore, the antimicrobial activity of the vesicles was tested while using different bacterial strains and a yeast: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans, respectively. The vesicles were small in size (~140 nm), slightly polydispersed (PI ~ 0.31), highly negatively charged (~ −73 mV), and able to incorporate high amounts of essential oil or citral (E% ~ 86%). Pompia essential oil and citral exhibited antimicrobial activity against all of the assayed microorganisms, with P. aeruginosa being the least sensitive. Citral was slightly more effective than pompia essential oil against E. coli, S. aureus, and C. albicans. The incorporation of citral in vesicles improved its antifungal activity against C. albicans.

scholarly journals Bioactive Compounds in Aegopodium podagraria Leaf Extracts and Their Effects against Fluoride-Modulated Oxidative Stress in the THP-1 Cell Line

2021 ◽  
Vol 14 (12) ◽  
pp. 1334
Author(s):  
Karolina Jakubczyk ◽  
Agnieszka Łukomska ◽  
Sylwester Czaplicki ◽  
Anna Wajs-Bonikowska ◽  
Izabela Gutowska ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Essential Oil ◽  
Cell Line ◽  
Antioxidant Properties ◽  
Total Content ◽  
Antioxidant Potential ◽  
Leaf Extracts ◽  
Positive Effects

Aegopodium podagraria L. (goutweed), a member of the Apiaceae family, is a common perennial plant found all around the world that has been used in folk medicine since antiquity. Goutweed leaves contain polyacetylenes, essential oils, mono- and sesquiterpenes, vitamins, macro- and microelements, and phenolic compounds. In spite of its many health-promoting properties, including antioxidant effects, the plant has not been thoroughly studied. The aim of this study was to investigate the antioxidant properties of different goutweed leaf extracts and their effects on the THP-1 cell line, and also to describe the chemical characteristics of goutweed. Falcarinol and falcarindiol and essential oil were determined by gas chromatography coupled with mass spectrometry. Spectrophotometry was used to measure the total content of polyphenols and antioxidant activity–by DPPH and FRAP methods. Oxidative stress in THP-1 cells was induced via sodium fluoride. Then, goutweed leaf extracts were added to evaluate their influence on antioxidant potential (ABTS) and the activity of antioxidant enzymes. Confocal microscopy was used to visualise the production of cytoplasmic and mitochondrial reactive oxygen species (ROS) and for in vitro imaging of apoptosis. The ethanol extracts have a high total content of polyphenols, polyacetylenes, and essential oil, as well as high antioxidant potential. The main volatiles represented diverse chemical groups, which are both oxygenated derivatives of sesquiterpenes and monoterpenes. We also demonstrated positive effects of the high antioxidant potential and increased activity of antioxidant enzymes on cell cultures under severe fluoride-induced oxidative stress. Extraction at 80 ℃ and the use of ethanol as a solvent increased the antioxidant capacity of the extract. The leaves of Aegopodium podagraria may serve as a valuable source of antioxidants in the daily diet and assist in the prevention and treatment of oxidative stress-mediated conditions, e.g., inflammatory conditions, cardiovascular diseases, neurodegenerative diseases, and even obesity.

In vitro and in vivo effect of Citrus limon essential oil against sarcoptic mange in rabbits

2016 ◽  
Vol 115 (8) ◽  
pp. 3013-3020 ◽  
Author(s):  
S. M. Aboelhadid ◽  
Lilian N. Mahrous ◽  
Shimaa A. Hashem ◽  
E. M. Abdel-Kafy ◽  
Robert J. Miller
Keyword(s):  
Essential Oil ◽  
Sarcoptic Mange ◽  
Citrus Limon

scholarly journals Chemical composition and in vitro antioxidant potential of essential oil and rhizome extracts of Curcuma amada Roxb

2016 ◽  
Vol 2 (1) ◽  
pp. 1168067 ◽  
Author(s):  
Anita Tamta ◽  
Om Prakash ◽  
H. Punetha ◽  
A.K. Pant ◽  
John Spencer
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Antioxidant Potential ◽  
Curcuma Amada

scholarly journals Acetylcholinesterase Inhibition and Antioxidant Activity of Essential Oils from Schinus Areira L. and Schinus Longifolia (Lindl.) Speg

2009 ◽  
Vol 4 (6) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Ana P. Murray ◽  
María S. Vela Gurovic ◽  
Silvana A. Rodriguez ◽  
María G. Murray ◽  
Adriana A. Ferrero
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Antioxidant Activities ◽  
Acetylcholinesterase Inhibition ◽  
Radical Scavenger ◽  
Radical Scavenger Activity ◽  
Scavenger Activity

The essential oils of Schinus areira L. and S. longifolia (Lindl.) Speg. (Anacardiaceae) have been studied for their in vitro anti-acetylcholinesterase and antioxidant activities. The chemical composition of the oils obtained by hydrodistillation was determined by GC-MS. Fruit and leaf oils of S. areira were analyzed separately. The essential oil from S. longifolia elicited marked enzymatic inhibition (IC50 = 20.0 ± 1.0 μg/mL) and showed radical scavenger activity (IC50 = 25.2 ± 2.4 μg/mL). The essential oil from S. areira leaves was more active than that of the fruits in both bioassays.

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