scholarly journals Emodin Induces Apoptotic Death in Murine Myelomonocytic Leukemia WEHI-3 CellsIn Vitroand Enhances Phagocytosis in Leukemia MiceIn Vivo

2011 ◽  
Vol 2011 ◽  
pp. 1-13 ◽  
Author(s):  
Yuan-Chang Chang ◽  
Tung-Yuan Lai ◽  
Chun-Shu Yu ◽  
Hung-Yi Chen ◽  
Jai-Sing Yang ◽  
...  
Keyword(s):  
Chromatin Condensation ◽  
Leukemia Cell ◽  
Cell Types ◽  
Leukemia Cell Line ◽  
Apoptotic Death ◽  
Murine Leukemia Cell ◽  
Available Information ◽  
Murine Leukemia

Emodin is one of major compounds in rhubarb (Rheum palmatumL.), a plant used as herbal medicine in Chinese population. Although many reports have shown that emodin exhibits anticancer activity in many tumor cell types, there is no available information addressing emodin-affected apoptotic responses in the murine leukemia cell line (WEHI-3) and modulation of the immune response in leukemia mice. We investigated that emodin induced cytotoxic effectsin vitroand affected WEHI-3 cellsin vivo. This study showed that emodin decreased viability and induced DNA fragmentation in WEHI-3 cells. Cells after exposure to emodin for 24 h have shown chromatin condensation and DNA damage. Emodin stimulated the productions of ROS and Ca2+and reduced the level ofΔΨmby flow cytometry. Our results from Western blotting suggest that emodin triggered apoptosis of WEHI-3 cells through the endoplasmic reticulum (ER) stress, caspase cascade-dependent and -independent mitochondrial pathways. Inin vivostudy, emodin enhanced the levels of B cells and monocytes, and it also reduced the weights of liver and spleen compared with leukemia mice. Emodin promoted phagocytic activity bymonocytesandmacrophagesin comparison to the leukemia mice group. In conclusions, emodin induced apoptotic death in murine leukemia WEHI-3 cells and enhanced phagocytosis in the leukemia animal model.

Thyroid hormone responsiveness of the L1210 murine leukemia cell line

1992 ◽  
Vol 126 (4) ◽  
pp. 374-377 ◽  
Author(s):  
J Brtko ◽  
P Filipčík ◽  
J Knopp ◽  
V Sedláková ◽  
Keyword(s):  
Cell Cycle ◽  
Growth Rate ◽  
Protein Phosphorylation ◽  
Nuclear Receptors ◽  
Leukemia Cell ◽  
Leukemia Cell Line ◽  
L1210 Leukemia ◽  
Murine Leukemia Cell ◽  
Murine Leukemia

The presence of saturable and high affinity 3,5,3′-triiodothyronine (T3) binding sites was demonstrated in LI 210 murine leukemia cell nuclei. Scatchard analysis revealed one class of receptors for T3 with Ka = 2.187 × 109l/mol and a maximum binding capacity (Bmax) of 3.96 fmol/106 cells. The effects of T3 on protein phosphorylation and growth rate of L1210 cells were investigated in a medium containing T3-depleted fetal calf serum. T3 was observed to be effective in enhancing protein phosphorylation (153.06%±5.99 sd) compared to cells grown in the absence of T3 (81.49%±13.50 sd). Moreover, in the presence of high T3 concentration (11.15 nmol/l) T3 was found to significantly increase the cell growth rate. In addition, the T3 receptor-associated alterations during the cell cycle, as measured by flow cytometry, suggest that the presence of T3 receptors becomes evident during the late G3 phase of the cell cycle, and T3 receptor numbers increase during the S phase. These results suggest that in in vitro conditions representing high T3 concentration, the number of L1210 leukemia cells may be increased by T3 via nuclear receptors. The L1210 leukemia cell line may serve as a convenient tool for in vitro studies of nuclear receptors and/or mechanism of action of T3. The binding affinity of T3 receptors is similar to that found in rat hepatocytes or human lymphocytes.

scholarly journals Induction of erythroid differentiation by inhibition of Ras/ERK pathway in a Friend murine leukemia cell line

Oncogene ◽  
2000 ◽  
Vol 19 (12) ◽  
pp. 1500-1508 ◽  
Author(s):  
Tomoko Matsuzaki ◽  
Ken-ichi Aisaki ◽  
Yasuko Yamamura ◽  
Makoto Noda ◽  
Yoji Ikawa
Keyword(s):  
Cell Line ◽  
Leukemia Cell ◽  
Erythroid Differentiation ◽  
Leukemia Cell Line ◽  
Erk Pathway ◽  
Murine Leukemia Cell ◽  
Murine Leukemia

scholarly journals Curcumin and Its Analogue Induce Apoptosis in Leukemia Cells and Have Additive Effects with Bortezomib in Cellular and Xenograft Models

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
L. I. Nagy ◽  
L. Z. Fehér ◽  
G. J. Szebeni ◽  
M. Gyuris ◽  
P. Sipos ◽  
...  
Keyword(s):  
Leukemia Cell ◽  
Leukemic Cells ◽  
Leukemia Cell Line ◽  
Leukemia Cells ◽  
Great Promise ◽  
Human Leukemia ◽  
Human Leukemia Cell Line ◽  
Apoptotic Effects

Combination therapy of bortezomib with other chemotherapeutics is an emerging treatment strategy. Since both curcumin and bortezomib inhibit NF-κB, we tested the effects of their combination on leukemia cells. To improve potency, a novel Mannich-type curcumin derivative, C-150, was synthesized. Curcumin and its analogue showed potent antiproliferative and apoptotic effects on the human leukemia cell line, HL60, with different potency but similar additive properties with bortezomib. Additive antiproliferative effects were correlated well with LPS-induced NF-κB inhibition results. Gene expression data on cell cycle and apoptosis related genes, obtained by high-throughput QPCR, showed that curcumin and its analogue act through similar signaling pathways. In correlation with in vitro results similar additive effect could be obsereved in SCID mice inoculated systemically with HL60 cells. C-150 in a liposomal formulation given intravenously in combination with bortezomib was more efficient than either of the drugs alone. As our novel curcumin analogue exerted anticancer effects in leukemic cells at submicromolar concentration in vitro and at 3 mg/kg dose in vivo, which was potentiated by bortezomib, it holds a great promise as a future therapeutic agent in the treatment of leukemia alone or in combination.

scholarly journals Cytostatic Effect of Deoxyspergualin on a Murine Leukemia Cell Line L1210

1991 ◽  
Vol 82 (10) ◽  
pp. 1065-1068 ◽  
Author(s):  
Masaharu Hiratsuka ◽  
Hiroshi Kuramochi ◽  
Katsutoshi Takahashi ◽  
Tomio Takeuchi ◽  
Mitsuo Mitsuo
Keyword(s):  
Cell Line ◽  
Leukemia Cell ◽  
Leukemia Cell Line ◽  
Cytostatic Effect ◽  
Murine Leukemia Cell ◽  
Murine Leukemia

scholarly journals Characterization of a Mutation in the Reduced Folate Carrier in a Transport Defective L1210 Murine Leukemia Cell Line

1995 ◽  
Vol 270 (39) ◽  
pp. 22974-22979 ◽  
Author(s):  
Kevin E. Brigle ◽  
Michael J. Spinella ◽  
Esteban E. Sierra ◽  
I. David Goldman
Keyword(s):  
Cell Line ◽  
Leukemia Cell ◽  
Leukemia Cell Line ◽  
Reduced Folate Carrier ◽  
Murine Leukemia Cell ◽  
Murine Leukemia

scholarly journals Proliferation and differentiation of human myeloid leukemic cells in immunodeficient mice: electron microscopy and cytochemistry

Blood ◽  
1984 ◽  
Vol 63 (5) ◽  
pp. 1015-1022 ◽  
Author(s):  
EA Machado ◽  
DA Gerard ◽  
CB Lozzio ◽  
BB Lozzio ◽  
JR Mitchell ◽  
...  
Keyword(s):  
Nude Mice ◽  
Leukemia Cell ◽  
Leukemic Cells ◽  
Leukemia Cell Line ◽  
Leukemia Cells ◽  
Human Leukemia ◽  
Immunodeficient Mice ◽  
Human Leukemia Cells

Abstract To study the influence of a biologic environment on cultured human leukemia cells, KG-1, KG-1a, and HL-60 cells were inoculated subcutaneously into newborn nude mice. The cells developed myelosarcomas at the site of inoculation and in lungs and kidneys. KG-1 and HL-60 myelosarcomas were successfully passaged through adult nude mice, whereas KG-1a tumors proliferated only after transplantation into newborn hosts. The human nature of the cells forming myelosarcomas in mice was assessed by chromosomal analyses and detection of cross- reactivity with an antibody to the human leukemia cell line K562. We undertook electron microscopic and cytochemical examinations of the cells proliferating in vitro and in the mice. The granules of KG-1 cells in vivo did not react for acid phosphatase, as observed in vitro, and the HL-60 cells proliferating in mice lost the perinuclear myeloperoxidase (MPO) demonstrated in cultured cells. Although the influence of an in vivo selection of cell subpopulations cannot be ruled out, the enzymatic changes are compatible with induced cell differentiation. Conclusive evidence of differentiation in vivo was observed in the KG-1a cell subline. The undifferentiated KG-1a blasts developed cytoplasmic granules and synthesized MPO during proliferation in vivo. These observations indicate that human leukemia cells from established cell lines proliferate in nude mice and may acquire new differentiated properties in response to the in vivo environment.

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