scholarly journals Some Observations on Carbon Nanotubes Susceptibility to Cell Phagocytosis

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Aneta Fraczek-Szczypta ◽  
Elzbieta Menaszek ◽  
Stanislaw Blazewicz

The aim of this study was to assess the influence of different types of carbon nanotubes (CNTs) on cell phagocytosis. Three kinds of carbon nanotubes: single-walled carbon nanohorns (SWCNHs), multiwalled carbon nanotubes (MWCNTs), and ultra-long single-walled carbon nanotubes (ULSWCNTs) before and after additional chemical functionalization were seeded with macrophage cell culture. Prior to biological testing, the CNTs were subjected to dispersion process with the use of phosphate buffered solution (PBS) and PBS containing surfactant (Tween 20) or dimethyl sulfoxide (DMSO). The results indicate that the cells interaction with an individual nanotube is entirely different as compared to CNTs in the form of aggregate. The presence of the surfactant favors the CNTs dispersion in culture media and facilitates phagocytosis process, while it has disadvantageous influence on cells morphology. The cells phagocytosis is a more effective for MWCNTs and SWCNHs after their chemical functionalization. Moreover, these nanotubes were well dispersed in culture media without using DMSO or surfactant. The functionalized carbon nanotubes were easily dispersed in pure PBS and seeded with cells.

2015 ◽  
Vol 2015 ◽  
pp. 1-9
Author(s):  
Ana Cláudia C. de Paula ◽  
Gustavo A. M. Sáfar ◽  
Alfredo M. Góes ◽  
Marcelo P. Bemquerer ◽  
Marcos A. Ribeiro ◽  
...  

Human adipose-derived stem cells (hASCs) are an attractive cell source for therapeutic applicability in diverse fields for the repair and regeneration of damaged or malfunctioning tissues and organs. There is a growing number of cell therapies using stem cells due to their characteristics of modulation of immune system and reduction of acute rejection. So a challenge in stem cells therapy is the delivery of cells to the organ of interest, a specific site. The aim of this paper was to investigate the effects of a supramolecular assembly composed of single-walled carbon nanotubes (SWCNT), molecular magnets (lawsone-Co-phenanthroline), and a synthetic peptide (FWYANHYWFHNAFWYANHYWFHNA) in the hASCs cultures. The hASCs were isolated, characterized, expanded, and cultured with the SWCNT supramolecular assembly (SWCNT-MA). The assembly developed did not impair the cell characteristics, viability, or proliferation. During growth, the cells were strongly attached to the assembly and they could be dragged by an applied magnetic field of less than 0.3 T. These assemblies were narrower than their related allotropic forms, that is, multiwalled carbon nanotubes, and they could therefore be used to guide cells through thin blood capillaries within the human body. This strategy seems to be useful as noninvasive and nontoxic stem cells delivery/guidance and tracking during cell therapy.


Nanomaterials ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 818 ◽  
Author(s):  
Olga Sedelnikova ◽  
Olga Gurova ◽  
Anna Makarova ◽  
Anastasiya Fedorenko ◽  
Anton Nikolenko ◽  
...  

Filling of single-walled carbon nanotubes (SWCNTs) and extraction of the encapsulated species from their cavities are perspective treatments for tuning the functional properties of SWCNT-based materials. Here, we have investigated sulfur-modified SWCNTs synthesized by the ampoule method. The morphology and chemical states of carbon and sulfur were analyzed by transmission electron microscopy, Raman scattering, thermogravimetric analysis, X-ray photoelectron and near-edge X-ray absorption fine structure spectroscopies. Successful encapsulation of sulfur inside SWCNTs cavities was demonstrated. The peculiarities of interactions of SWCNTs with encapsulated and external sulfur species were analyzed in details. In particular, the donor–acceptor interaction between encapsulated sulfur and host SWCNT is experimentally demonstrated. The sulfur-filled SWCNTs were continuously irradiated in situ with polychromatic photon beam of high intensity. Comparison of X-ray spectra of the samples before and after the treatment revealed sulfur transport from the interior to the surface of SWCNTs bundles, in particular extraction of sulfur from the SWCNT cavity. These results show that the moderate heating of filled nanotubes could be used to de-encapsulate the guest species tuning the local composition, and hence, the functional properties of SWCNT-based materials.


Nanoscale ◽  
2012 ◽  
Vol 4 (20) ◽  
pp. 6434 ◽  
Author(s):  
Chiara Ingrosso ◽  
Giuseppe Valerio Bianco ◽  
Patrizia Lopalco ◽  
Michela Tamborra ◽  
Maria Lucia Curri ◽  
...  

2006 ◽  
Vol 326-328 ◽  
pp. 321-324 ◽  
Author(s):  
Yong Jae Kim ◽  
Sang Hwan Lee ◽  
Do Young Byun ◽  
Sang Joon Han ◽  
Ji Hye Yang ◽  
...  

We propose to use electrostatic drop-on-demand devices, with precise metering and accurate delivery capabilities, to manipulate single-walled carbon nanotubes suspended in solution. The conductivity of the solution increases significantly by the addition of anionic surfactants and nanotubes, generating favorable conditions for electrostatic ejection. This technique could find applications, combined with chemical functionalization, in the fabrication of carbon nanotube field emission devices as well as electronic circuits. [email protected]


Nanoscale ◽  
2016 ◽  
Vol 8 (38) ◽  
pp. 16916-16921 ◽  
Author(s):  
Yutaka Maeda ◽  
Shun Minami ◽  
Yuya Takehana ◽  
Jing-Shuang Dang ◽  
Shun Aota ◽  
...  

Controlling the distance of the addition site carbons is a simple method for controlling NIR PL and UCPL properties of SWNTs.


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