scholarly journals Thermodynamic and spectroscopic study on the binding of interaction anionic phthalocyanine with calf thymus DNA

2011 ◽  
Vol 26 (6) ◽  
pp. 357-365 ◽  
Author(s):  
Hamid Dezhampanah ◽  
Termeh Darvishzad ◽  
Mehrnaz Aghazadeh
Keyword(s):  
Ground State ◽  
Deoxyribonucleic Acid ◽  
Optical Absorption Spectra ◽  
Groove Binding ◽  
Base Pairs ◽  
Static Mode ◽  
Absorption And Fluorescence Spectroscopy ◽  
Calf Thymus ◽  
Axial Bond ◽  
Ct Dna

The interaction between anionic form of copper (II) N,N',N",N'"-tetrasulfonated phthalocyanine Cu (tspc) and to calf thymus deoxyribonucleic acid (ct-DNA) is investigated by measuring UV-vis absorption and fluorescence spectroscopy in phosphate buffer. The binding constant and stoichiometry were determined by analysis of optical absorption spectra of phthalocyanine at various ct-DNA concentrations using SQUAD software. The static mode of fluorescence quenching of phthalocyanine by calf thymus deoxyribonucleic acid indicates the formation of a ground-state complex. The formation of ground-state complex is a spontaneous molecular interaction procedure in which outside groove binding through the formation of an axial bond between the base pairs of nucleotide and Cu in the central core of phthalocyanine.

Surfactant Complex Binding to DNA Interaction Study: Controlling Hydrophobicity in β-Cyclodextrin–DNA Binding Reactions

2020 ◽  
pp. 318-332
Author(s):  
Nagaraj Karuppiah ◽  
◽  
Muthukumaran Pakkirisamy ◽  
Gunasekaran Gladwin ◽  
Keyword(s):  
Dna Interaction ◽  
Spectroscopic Studies ◽  
Calf Thymus Dna ◽  
Aliphatic Chain ◽  
Groove Binding ◽  
Base Pairs ◽  
Surfactant Complex ◽  
Calf Thymus ◽  
Frame Work ◽  
Ct Dna

The interaction of cis-[Co(phen)2(TA)2](ClO4)3, a cationic surfactant complex (phen = 1-10 phenanthroline, TA= Tetradecylamine), with calf thymus DNA has been studied by physici-chemical techniques. The spectroscopic studies together with cyclic voltammetry and viscosity experiments support that the surfactant-cobalt(III) complex binds to calf thymus DNA (CT DNA) by intercalation through the aliphatic chain present in the complex into the base pairs of DNA. The presence of phenanthroline ligand with larger -frame work may also enhance intercalation. Besides the effect of binding of surfactant cobalt(III) complex to DNA in presence of -cyclodextrin has also studied. In presence of -cyclodextrin the binding occur through surface and (or) groove binding. The complex was investigated as one of the potential

scholarly journals Affinity studies of hypocrellin B and mono-cysteine substituted hypocrellin B with CT-DNA using spectroscopic methods

2005 ◽  
Vol 19 (5,6) ◽  
pp. 259-266 ◽  
Author(s):  
Shaohua Wei ◽  
Jiahong Zhou ◽  
Yuying Feng ◽  
Deyin Huang ◽  
Xuesong Wang ◽  
...  
Keyword(s):  
Deoxyribonucleic Acid ◽  
Double Helix ◽  
Cd Spectroscopy ◽  
Base Stacking ◽  
Calf Thymus ◽  
Visible Spectra ◽  
Hypocrellin B ◽  
Uv Visible ◽  
Dna Double Helix ◽  
Ct Dna

The interaction of anticancer drug hypocrellin B (HB) and mono-cysteine substituted hypocrellin B (MCHB) with calf thymus deoxyribonucleic acid (CT-DNA) has been investigated using spectral methods. The results of UV–visible spectra showed that the HB and MCHB can intercalate into the base-stacking domain of the CT-DNA double helix. Further studies based on fluorescence spectroscopy and circular dichroism (CD) spectroscopy also supported the intercalation mechanism.

scholarly journals Interaction of Duloxetine Hydrochloride with Deoxyribonucleic Acid Measured by Fluorescence Spectroscopy

2016 ◽  
Vol 14 (2) ◽  
pp. 199-206
Author(s):  
Raznin Akter Joly ◽  
Md Reazul Islam ◽  
Sonia Sultana ◽  
Asma Rahman ◽  
Md Zakir Sultan ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Deoxyribonucleic Acid ◽  
Antidepressant Drug ◽  
Living Organism ◽  
Binding Mode ◽  
Duloxetine Hydrochloride ◽  
Calf Thymus ◽  
Binding Forces ◽  
Ct Dna ◽  
Hypochromic Effect

Interactions with many clinically active therapeutic agents with DNA are well studied and it is necessary to decipher the structure of DNA and to investigate the pathological implications of those molecules in living organism. This study investigated the interaction of antidepressant drug Duloxetine-hydrochloride (DLX) with calf thymus DNA (ct-DNA). The interaction of DLX with ct-DNA was studied employing fluorescence spectroscopy. Hypochromic effect was found in the absorption spectra of duloxetine, and its wavelength had no shift in the presence of DNA indicating external binding mode of duloxetine to DNA. Fluorescence spectroscopic results showed the quenching of fluorescence intensity of DLX in presence of DNA indicating the interaction between DLX and DNA. Hydrophobic interaction and hydrogen bonding played the dominating role in DLX-DNA binding and binding forces also indicate the binding site of duloxetine to be at the minor groove of DNA.Dhaka Univ. J. Pharm. Sci. 14(2): 199-206, 2015 (December)

Synthesis, Characterization and Bioactivity of Three Carboxylic Arylhydrazone Compounds

2020 ◽  
Vol 42 (1) ◽  
pp. 149-149
Author(s):  
Fengying Chen Fengying Chen ◽  
Wangting Wu Wangting Wu ◽  
Zhenguo Jin Zhenguo Jin ◽  
Shuiyang He Shuiyang He ◽  
Chengfang Qiao and Fei Yuan Chengfang Qiao and Fei Yuan
Keyword(s):  
Hydrogen Bonds ◽  
Propionic Acid ◽  
Elemental Analysis ◽  
Fluorescence Spectra ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Groove Binding ◽  
Calf Thymus ◽  
Ct Dna ◽  
Wheat Rust

Three novel carboxylic arylhydrazone compounds named 2- oxo propionic acid terephthalal acyl dihydrazone (1), 2-ketoglutaric acid terephthalal acyl dihydrazone (2) and 2-ketoglutaric acid salicyl- hydrazone (3) were prepared and characterized by elemental analysis, IR and 1H NMR. The antibacterial activities of 1 and 2 against wheat rust and coliform were investigated. The results showed that 1 had more excellent antibacterial properties than 2 against both wheat rust and coliform. In addition, the title compounds interaction with calf-thymus DNA (CT-DNA) were measured by fluorescence spectra method which indicating that they combined with CT-DNA by groove binding through hydrogen bonds.

The Use Of Molecular Docking And Spectroscopic Methods For Investigation Of The Interaction Between Regorafenib With Human Serum Albumin (HSA) And Calf Thymus DNA (Ct-DNA) In The Presence Of Different Site Markers

2020 ◽  
Vol 27 ◽  
Author(s):  
Hamid Tanzadehpanah ◽  
Hanie Mahaki ◽  
Mohammadreza Moradi ◽  
Saeid Afshar ◽  
Neda Hosseinpour Moghadam ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Human Serum Albumin ◽  
Zeta Potential ◽  
Serum Albumin ◽  
Human Serum ◽  
Calf Thymus Dna ◽  
Static Mode ◽  
Calf Thymus ◽  
Site Markers ◽  
Ct Dna

Background: Interactions of drugs with DNA and proteins may modify their biological activities and conformations, which effect transport and biological metabolism of drugs. Objective: In this study the interaction of anticancer drug regorafenib (REG) with calf thymus-DNA (ct-DNA) and human serum albumin (HSA) has been investigated. Methods: Hence, for the first time, it was discovered interaction between REG with DNA and HSA using multispectroscopic, zeta potential measurements and molecular docking method. Results and Discussion: DNA displacement studies showed that REG does not have any effect on acridine orange and methylene blue bound DNA, though it was substantiated by displacement studies with Hoechst (as groove binder). Furthermore, the different concentrations of REG induce slight changes in the viscosity of ct-DNA. Zeta potential parameters indicated that hydrophobic interaction plays a major role in the DNA-REG complex. Results obtained from molecular docking demonstrate that the REG prefers to bind on the minor groove of DNAs than that of the major groove. Binding properties of HSA reveal that intrinsic fluorescence of HSA could be quenched by REG in a static mode. The competitive experiments in the presence of warfarin and ibuprofen (as site markers) suggested that the binding site of REG to HSA was most probably located in the subdomain IIA. Measurements of the zeta potential indicated that REG bound to HSA mainly by both electrostatic and hydrophobic interactions. It was found on docking procedures that REG could fit well into HSA subdomain IIA, which confirmed the experimental results. Conclusion: In conclusion, REG can be delivered by HSA in a circulatory system and affect DNA as potential target.

scholarly journals DNA Binding and Photocleavage Studies of Cobalt(III) Polypyridine Complexes: [Co(en)2PIP]3+, [Co(en)2IP]3+, and [Co(en)2phen-dione]3+

2007 ◽  
Vol 2007 ◽  
pp. 1-8 ◽  
Author(s):  
Penumaka Nagababu ◽  
S. Satyanarayana
Keyword(s):  
Plasmid Dna ◽  
Emission Spectroscopy ◽  
Spectroscopic Studies ◽  
Calf Thymus Dna ◽  
Dna Melting ◽  
Base Pairs ◽  
Viscosity Measurements ◽  
Calf Thymus ◽  
Polypyridine Complexes ◽  
Ct Dna

In this paper, three complexes of type[Co(en)2PIP]3+(PIP=2-phenylimidazo[4,5-f][1,10,] phenanthroline)(1),[Co(en)2IP]3+(IP=imidazo[4,5-f][1,10,] phenanthroline)(2), and[Co(en)2phen-dione]3+(1,10 phenanthroline 5,6,dione)(3) have been synthesized and characterized by UV/VIS, IR,1HNMR spectral methods. Absorption spectroscopy, emission spectroscopy, viscosity measurements, and DNA melting techniques have been used for investigating the binding of these two complexes with calf thymus DNA, and photocleavage studies were used for investigating these binding of these complexes with plasmid DNA. The spectroscopic studies together with viscosity measurements and DNA melting studies support that complexes 1 and 2 bind to CT DNA(=calf thymus DNA) by intercalation mode via IP or PIP into the base pairs of DNA, and complex 3 is binding as groove mode. Complex 1 binds more avidly to CT DNA than 2 and 3 which is consistent with the extended planar ringπsystem of PIP. Noticeably, the two complexes have been found to be efficient photosensitisers for strand scissions in plasmid DNA.

scholarly journals Effects of Polycyclic Aromatic Hydrocarbon Pendant-Armed Ligands on the Catecholase Activity of Dinuclear Copper(II) Complexes

2021 ◽  
Author(s):  
Marcos da Silva ◽  
Renata Heying ◽  
Letícia da Silva ◽  
Adailton Bortoluzzi ◽  
Rosely Peralta ◽  
...  
Keyword(s):  
Deoxyribonucleic Acid ◽  
Catechol Oxidase ◽  
X Ray ◽  
X Ray Crystallography ◽  
Ph Values ◽  
Biomimetic Catalyst ◽  
Calf Thymus ◽  
Polycyclic Aromatic ◽  
Ct Dna ◽  
Potential Use

Herein, we present three new binuclear copper(II) complexes containing different polycyclic aromatic hydrocarbons, able to catalyze the oxidation of 3,5-di-tert-butylcathecol, a model substrate for catechol oxidase. The ligands and complexes were successful characterized in solid and solution states. The structure of C1 was determined by X-ray crystallography and it contains a [CuII2(L1-μ-phenoxo)(OAc)(H2O)2] unit, with two coppers in a pyramidal square geometry and a large distance of 3.715 Å between the copper(II) centers. All complexes (C1, C2 and C3) were found to be effective catalysts in the oxidation of 3,5-di-tert-butylcathecol to its quinone and C1 provided the highest catalytic constants at the three pH values studied. In addition to their potential use as a biomimetic catalyst in catechol oxidation, the interaction of these complexes with deoxyribonucleic acid from calf thymus (CT-DNA) was also studied, and C3 showed the greatest affinity with nucleic acids.

scholarly journals Synthesis, Characterisation, Interaction with DNA, Cytotoxicity, and Apoptotic Studies of Ruthenium(II) Polypyridyl Complexes

2014 ◽  
Vol 67 (2) ◽  
pp. 225 ◽  
Author(s):  
C. Shobha Devi ◽  
Penumaka Nagababu ◽  
V. Venkat Reddy ◽  
V. Sateesh ◽  
A. Srishailam ◽  
...  
Keyword(s):  
Base Pairs ◽  
Polypyridyl Complexes ◽  
Ancillary Ligands ◽  
Spectrophotometric Methods ◽  
Dna Base ◽  
Calf Thymus ◽  
Pbr322 Dna ◽  
Dna Base Pairs ◽  
Ic50 Values ◽  
Ct Dna

We report the synthesis and characterisation of two new ruthenium(ii) polypyridyl complexes containing monodentate ancillary ligands [Ru(L)4(4HEPIP)], where L = 4-aminopyridine (1) or pyridine (2) and 4HEPIP = 2-(4-hydroxy-3-ethoxyphenyl)-1H-imidazo[4,5-f][1,10](phenanthroline). These complexes were characterised by elemental analysis and ultraviolet-visible, infrared, and 1H NMR spectroscopy. The binding properties of the two complexes towards calf thymus (CT)-DNA were investigated with different spectrophotometric methods, viscosity measurements, and salt dependent studies. Experimental results indicated that the complexes interact with CT-DNA base pairs by intercalation. Upon irradiation at 365 nm, these complexes efficiently cleave pBR322 DNA from super coiled form I to nicked form II. Their cytotoxicity on different cancer cell lines such as A549, Du145, and HeLa was investigated. The IC50 values are 39.5, 28.3, and 27.3 μM for complex 1, and 55, 67.9, and 47.9 μM for complex 2 respectively. Cellular uptake and apoptosis induced by these complexes was also studied.

scholarly journals Study of Interaction of Dextromethorphan Hydrobromide with Deoxyribonucleic Acid by Fluorescence Quenching

2016 ◽  
Vol 19 (2) ◽  
pp. 197-205
Author(s):  
Toufiq Ul Amin ◽  
Md Reazul Islam ◽  
Md Zakir Sultan ◽  
Sonia Sultana ◽  
Md Saiful Islam ◽  
...  
Keyword(s):  
Deoxyribonucleic Acid ◽  
Living Organism ◽  
Pharmaceutical Journal ◽  
Binding Mode ◽  
Spectroscopic Techniques ◽  
Calf Thymus ◽  
Different Temperatures ◽  
Binding Forces ◽  
Ct Dna ◽  
Dextromethorphan Hydrobromide

Interactions with many clinically active therapeutic agents with deoxyribonucleic acid (DNA) are well studied and it expedites deciphering the structure of DNA and to investigate the pathological implication of those molecules in a living organism. The interaction of dextromethorphan hydrobromide (DEX) with calf thymus DNA (ct DNA) was studied employing UV absorption and fluorescence spectroscopic techniques. The binding affinity of DEX to DNA was calculated at different temperatures and the stoichiometry of binding was characterized to be about 1 dextromethorphan molecule per nucleotide. Hypochromic effect was found in the absorption spectra of dextromethorphan, and its wavelength had no shift in the presence of DNA indicating external binding mode of dextromethorphan to DNA. Quenching constants 3532 L/ mol and 12446L/ mol at 298 K and 308 K respectively with correlation co-efficient of 0.974 and 0.976, using Stern-Volmer equation and the quenching mechanism was found to be dynamic. Fluorescence spectroscopic results showed the quenching of fluorescence intensity of DEX in the presence of DNA, indicating the interaction between DEX and DNA. Based on this, hydrophobic interaction were found to play the dominating role in DEX-DNA binding and those binding forces also indicate the binding site of dextromethorphan to be in the minor groove of DNA.Bangladesh Pharmaceutical Journal 19(2): 197-205, 2016

Multi-spectroscopic and molecular modelling studies on the interaction of esculetin with calf thymus DNA

2015 ◽  
Vol 11 (2) ◽  
pp. 522-531 ◽  
Author(s):  
Tarique Sarwar ◽  
Mohammed Amir Husain ◽  
Sayeed Ur Rehman ◽  
Hassan Mubarak Ishqi ◽  
Mohammad Tabish
Keyword(s):  
Molecular Modelling ◽  
In Silico ◽  
Minor Groove ◽  
Minor Groove Binding ◽  
Groove Binding ◽  
Calf Thymus ◽  
Molecular Modelling Studies ◽  
Modelling Studies ◽  
Ct Dna

Minor groove binding of esculetin with Ct-DNA was established by a series of in vitro experiments and in silico analyses.

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