scholarly journals Oligodeoxynucleotide Containing Disulphide Bond Stabilizes Triplex DNA Structure

2011 ◽  
Vol 8 (2) ◽  
pp. 507-512 ◽  
Author(s):  
Ajay Kumar
Keyword(s):  
Melting Temperature ◽  
Dna Structure ◽  
Disulphide Bond ◽  
Triplex Dna ◽  
Duplex Dna ◽  
Ph Values ◽  
Triplex Formation ◽  
The Stability ◽  
Better Than

The triplex formation of disulphide containing oligonucleotide with duplex DNA using melting temperature studies is reported. The stability of the triplex formed with disulphide containing oligonucleotide was compared with unmodified oligonucleotide and C-5 propyne deoxyuridine containing oligonucleotide. Melting temperature (Tm) values of the triplexes formed with disulphide containing oligonucleotide were found to be 37°C and 46°C at pH 7 and 6 respectively. The triplexes formed with C-5 propyne deoxyuridine substituted oligonucleotide showed Tmvalues at 27°C and 42°C at pH 7 and 6. The Tmvalues of the triplexes formed with unmodified oligonucleotide were found to be 18°C and 32°C at pH 7 and 6 respectively. This clearly demonstrates that disulphide containing oligonucleotide stabilzes triplex DNA structure better than unmodified oligonucleotide as well as C-5 propyne deoxyuridine containing oligonucleotide when targeted with duplex DNA at both the mentioned pH values.

scholarly journals Dispersion of Carbon Nanotubes with “Green” Detergents

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2908
Author(s):  
Kazuo Umemura ◽  
Ryo Hamano ◽  
Hiroaki Komatsu ◽  
Takashi Ikuno ◽  
Eko Siswoyo
Keyword(s):  
Carbon Nanotubes ◽  
Sodium Dodecyl ◽  
Detergent Solution ◽  
Absorbance Spectroscopy ◽  
Fundamental Research ◽  
The Stability ◽  
Single Walled Cnts ◽  
Better Than ◽  
Walled Cnts ◽  
Cnt Suspensions

Solubilization of carbon nanotubes (CNTs) is a fundamental technique for the use of CNTs and their conjugates as nanodevices and nanobiodevices. In this work, we demonstrate the preparation of CNT suspensions with “green” detergents made from coconuts and bamboo as fundamental research in CNT nanotechnology. Single-walled CNTs (SWNTs) with a few carboxylic acid groups (3–5%) and pristine multi-walled CNTs (MWNTs) were mixed in each detergent solution and sonicated with a bath-type sonicator. The prepared suspensions were characterized using absorbance spectroscopy, scanning electron microscopy, and Raman spectroscopy. Among the eight combinations of CNTs and detergents (two types of CNTs and four detergents, including sodium dodecyl sulfate (SDS) as the standard), SWNTs/MWNTs were well dispersed in all combinations except the combination of the MWNTs and the bamboo detergent. The stability of the suspensions prepared with coconut detergents was better than that prepared with SDS. Because the efficiency of the bamboo detergents against the MWNTs differed significantly from that against the SWNTs, the natural detergent might be useful for separating CNTs. Our results revealed that the use of the “green” detergents had the advantage of dispersing CNTs as well as SDS.

scholarly journals Measurement of the Intestinal pH in Mice under Various Conditions Reveals Alkalization Induced by Antibiotics

Antibiotics ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 180
Author(s):  
Kouki Shimizu ◽  
Issei Seiki ◽  
Yoshiyuki Goto ◽  
Takeshi Murata
Keyword(s):  
Antibiotic Treatment ◽  
High Protein Diet ◽  
Intestinal Bacteria ◽  
High Protein ◽  
Protein Diet ◽  
Germ Free ◽  
Treatment Regimens ◽  
Ph Values ◽  
Specific Pathogen ◽  
The Stability

The intestinal pH can greatly influence the stability and absorption of oral drugs. Therefore, knowledge of intestinal pH is necessary to understand the conditions for drug delivery. This has previously been measured in humans and rats. However, information on intestinal pH in mice is insufficient despite these animals being used often in preclinical testing. In this study, 72 female ICR mice housed in SPF (specific pathogen-free) conditions were separated into nine groups to determine the intestinal pH under conditions that might cause pH fluctuations, including high-protein diet, ageing, proton pump inhibitor (PPI) treatment, several antibiotic treatment regimens and germ-free mice. pH was measured in samples collected from the ileum, cecum and colon, and compared to control animals. An electrode, 3 mm in diameter, enabled accurate pH measurements with a small amount of gastrointestinal content. Consequently, the pH values in the cecum and colon were increased by high-protein diet, and the pH in the ileum was decreased by PPI. Drastic alkalization was induced by antibiotics, especially in the cecum and colon. The alkalized pH values in germ-free mice suggested that the reduction in the intestinal bacteria caused by antibiotics led to alkalization. Alkalization of the intestinal pH caused by antibiotic treatment was verified in mice. We need further investigations in clinical settings to check whether the same phenomena occur in patients.

Study on Influence of Silicate Morphology Based on pH Value

2012 ◽  
Vol 454 ◽  
pp. 324-328
Author(s):  
Yan He ◽  
Ya Jing Liu ◽  
Yong Lin Cao ◽  
Li Xia Zhou
Keyword(s):  
Silicic Acid ◽  
Ph Value ◽  
Colorimetric Method ◽  
Absorption Spectrometry ◽  
Degree Of Polymerization ◽  
Raw Material ◽  
X Ray Diffraction ◽  
X Ray ◽  
Ph Values ◽  
The Stability

Infra-red absorption spectrometry, X-ray diffraction observations and characterization tests based on silicon molybdenum colorimetric method were used to investigate the optimal pH value controlling the stability of the silicic acid form. The experiment process was done by using sodium silicate as raw material. The results showed that the solution of silicate influenced the polymerization. The active silicic acid solution with a certain degree of polymerization was obtained by controlling the pH values.

scholarly journals Research on Generation Method of Grasp Strategy Based on DeepLab V3+ for Three-Finger Gripper

Information ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 278
Author(s):  
Sanlong Jiang ◽  
Shaobo Li ◽  
Qiang Bai ◽  
Jing Yang ◽  
Yanming Miao ◽  
...  
Keyword(s):  
Field Of View ◽  
Convolution Kernel ◽  
Parallel Connection ◽  
Multi Scale ◽  
Spatial Pyramid Pooling ◽  
The Stability ◽  
Parameter Values ◽  
Filter Layer ◽  
Complex Dataset ◽  
Better Than

A reasonable grasping strategy is a prerequisite for the successful grasping of a target, and it is also a basic condition for the wide application of robots. Presently, mainstream grippers on the market are divided into two-finger grippers and three-finger grippers. According to human grasping experience, the stability of three-finger grippers is much better than that of two-finger grippers. Therefore, this paper’s focus is on the three-finger grasping strategy generation method based on the DeepLab V3+ algorithm. DeepLab V3+ uses the atrous convolution kernel and the atrous spatial pyramid pooling (ASPP) architecture based on atrous convolution. The atrous convolution kernel can adjust the field-of-view of the filter layer by changing the convolution rate. In addition, ASPP can effectively capture multi-scale information, based on the parallel connection of multiple convolution rates of atrous convolutional layers, so that the model performs better on multi-scale objects. The article innovatively uses the DeepLab V3+ algorithm to generate the grasp strategy of a target and optimizes the atrous convolution parameter values of ASPP. This study used the Cornell Grasp dataset to train and verify the model. At the same time, a smaller and more complex dataset of 60 was produced according to the actual situation. Upon testing, good experimental results were obtained.

Attitude reconstruction from strap-down rate gyros using power series

2021 ◽  
pp. 1-19
Author(s):  
Habib Ghanbarpourasl
Keyword(s):  
Power Series ◽  
Taylor Series ◽  
Direction Cosine ◽  
Analytical Description ◽  
Double Precision ◽  
Angular Velocity Vector ◽  
Higher Order Terms ◽  
Direction Cosine Matrix ◽  
The Stability ◽  
Better Than

Abstract This paper introduces a power series based method for attitude reconstruction from triad orthogonal strap-down gyros. The method is implemented and validated using quaternions and direction cosine matrix in single and double precision implementation forms. It is supposed that data from gyros are sampled with high frequency and a fitted polynomial is used for an analytical description of the angular velocity vector. The method is compared with the well-known Taylor series approach, and the stability of the coefficients’ norm in higher-order terms for both methods is analysed. It is shown that the norm of quaternions’ derivatives in the Taylor series is bigger than the equivalent terms coefficients in the power series. In the proposed method, more terms can be used in the power series before the saturation of the coefficients and the error of the proposed method is less than that for other methods. The numerical results show that the application of the proposed method with quaternions performs better than other methods. The method is robust with respect to the noise of the sensors and has a low computational load compared with other methods.

USING MALIC ACID SOLUTIONS FOR THE HYDRATION OF VEGETABLE OILS AND LECITHIN PRODUCTION

2020 ◽  
Author(s):  
Л.А. МАРЧЕНКО ◽  
Т.Н. БОКОВИКОВА ◽  
Е.В. ЛИСОВАЯ ◽  
С.А. ИЛЬИНОВА ◽  
Е.П. ВИКТОРОВА
Keyword(s):  
Malic Acid ◽  
Complex Compounds ◽  
Point Of View ◽  
Magnesium Ions ◽  
Ph Values ◽  
Calcium And Magnesium ◽  
The Stability ◽  
The Cost ◽  
Calcium And Magnesium Ions

Исследована возможность применения яблочной кислоты (ЯК) в качестве гидратирующего агента для перевода негидратируемых форм фосфолипидов в гидратируемые. Выбор ЯК обусловлен ее стоимостью, которая в 1,5 раза меньше стоимости янтарной кислоты, широко применяемой в качестве гидратирующего агента, а также большей доступностью с точки зрения промышленного производства. Исследование процесса комплексообразования и определение состава комплексных соединений ЯК с ионами кальция и магния осуществляли методом потенциометрического титрования. Установлено, что внесение в раствор ЯК ионов кальция и магния приводит к снижению значений рН, что свидетельствует о наличии комплексообразования в указанных системах. Наиболее устойчивыми являются комплексы ионов кальция и магния с непротонированным лигандом при соотношении Ме2 : лиганд 1 : 1. В процессе комплексообразования ионы Ca2 и Mg2 вытесняют протоны только карбоксильных групп ЯК, которая с ионами кальция образует более устойчивые комплексы, чем с ионами магния. Показано, что устойчивость комплексов ЯК с ионами кальция и магния значительно выше, чем устойчивость комплексов фосфатидилсеринов и фосфатидных кислот с указанными ионами. Использование водных растворов ЯК в качестве гидратирующего агента позволит повысить эффективность процесса гидратации и увеличить выход фосфолипидов и, следовательно, готового продукта лецитина. The possibility of using malic acid (MA) as a hydrating agent for converting non-hydrated forms of phospholipids into hydrated ones has been investigated. The choice of MA is due to its cost, which is 1,5 times less than the cost of succinic acid, as well as greater availability from the point of view of industrial production. The study of the complexation process and determination of the composition of complex compounds of MA with calcium and magnesium ions was carried out by the method of potentiometric titration. It was found that the introduction of calcium and magnesium ions into the MA solution leads to a decrease in pH values, which indicates the presence of complexation in these systems. Complexes of calcium and magnesium ions with an unprotected ligand at a ratio of ME2 : ligand 1 : 1 are the most stable. In the process of complexing, Ca2 and Mg2 ions displace protons only of the carboxyl groups of MA, which forms more stable complexes with calcium ions than with magnesium ions. It is shown that the stability of MA complexes with calcium and magnesium ions is significantly higher than the stability of phosphatidylserine and phosphatidic acid complexes with these ions. Using water solutions of MA as a hydrating agent will increase the efficiency of the hydration process and increase the yield of phospholipids and, consequently, the finished product lecithin.

Empirical and Theoretical Evaluation of a Tree-Shaped DNA Nanostructure with a Looped Arm (L-DNA)

2021 ◽  
Vol 13 (8) ◽  
pp. 1452-1457
Author(s):  
Jeonghun Kim ◽  
So Yeon Ahn ◽  
Soong Ho Um
Keyword(s):  
Melting Temperature ◽  
Structural Stability ◽  
Building Block ◽  
Dna Structure ◽  
Secondary Structures ◽  
Theoretical Evaluation ◽  
Single Stranded Dna ◽  
Practical Applications ◽  
Dna Nanostructure ◽  
Specific Selectivity

Several nanostructures have been created with the advent of nanotechnology. DNA has been recognized as a new building block material in addition to its genetic coding role because of its unique features (e.g., intrinsic biocompatibility, precise tenability, specific selectivity). DNA can be organized into a variety of self-assembled nanomaterials including a sphere, a ball, and even an emoticon. In particular, a tree-shaped DNA structure possessing characteristic fractural patterns is easily controlled by size and functionality and can be exploited in various fields. Here, we report an empirical and theoretical evaluation of a Y-shaped tree DNA nanostructure with a looped arm (L-DNA). The synthesized L-DNAs were analyzed for thermal and structural stability. The melting temperature (Tm) of a Y-shaped DNA (Y-DNA) as a core unit and a model DNA nanostructure comprising of central Y-DNA and looped arm were measured individually. According to the complexity (e.g., increased length of the single stranded DNA (ssDNA) used), its yield suddenly decreased with the generation of ssDNAs with distinctive secondary structures. A complicated DNA product is predicted by considering the Tm of expected secondary structures, with increased Tm with respect to variation in salt concentrations. Therefore, the new DNA nanostructure may be utilized as a platform for various practical applications.

Magnetorheological fluid based on amorphous Fe-Si-B alloy magnetic particles

2021 ◽  
pp. 1045389X2110643
Author(s):  
Chuncheng Yang ◽  
Zhong Liu ◽  
Xiangyu Pei ◽  
Cuiling Jin ◽  
Mengchun Yu ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Field Strength ◽  
Rheological Property ◽  
Magnetic Particles ◽  
Annealing Treatment ◽  
Magnetorheological Fluid ◽  
The Stability ◽  
The Magnetic Field ◽  
Amorphous Particle ◽  
Better Than

Magnetorheological fluids (MRFs) based on amorphous Fe-Si-B alloy magnetic particles were prepared. The influence of annealing treatment on stability and rheological property of MRFs was investigated. The saturation magnetization ( Ms) of amorphous Fe-Si-B particles after annealing at 550°C is 131.5 emu/g, which is higher than that of amorphous Fe-Si-B particles without annealing. Moreover, the stability of MRF with annealed amorphous Fe-Si-B particles is better than that of MRF without annealed amorphous Fe-Si-B particles. Stearic acid at 3 wt% was added to the MRF2 to enhance the fluid stability to greater than 90%. In addition, the rheological properties demonstrate that the prepared amorphous particle MRF shows relatively strong magnetic responsiveness, especially when the magnetic field strength reaches 365 kA/m. As the magnetic field intensified, the yield stress increased dramatically and followed the Herschel-Bulkley model.

Durability of BFRP and Hybrid FRP Sheets under Freeze-Thaw Cycling

2010 ◽  
Vol 163-167 ◽  
pp. 3297-3300 ◽  
Author(s):  
Jia Wei Shi ◽  
Hong Zhu ◽  
Zhi Shen Wu ◽  
Gang Wu
Keyword(s):  
Mechanical Properties ◽  
Test Data ◽  
Mechanical Characteristics ◽  
Test Results ◽  
Freeze Thaw ◽  
Frp Composites ◽  
Test Parameters ◽  
The Stability ◽  
Better Than

Coupon tests were conducted to investigate the mechanical characteristics of basalt FRP (BFRP) sheet, basalt-carbon hybrid FRP sheets and the corresponding epoxy rein under the effect of freeze-thaw cycling. FRP sheets and epoxy rein coupons were subjected to up to 200 and 250 freeze-thaw cycles respectively. Test parameters included the number of freeze-thaw cycles and the types of FRP composites. Test results show that (1) BFRP sheet perform better than CFRP or GFRP sheets under high freeze-thaw cycles; (2) exposed hybrid FRP sheets not only show very little loss in mechanical properties, but also contribute to the stability of test data; (3) mechanical properties of rein epoxy decrease significantly with increasing freeze-thaw cycles.

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