scholarly journals The Mechanosensory Role of Primary Cilia in Vascular Hypertension

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Surya M. Nauli ◽  
Xingjian Jin ◽  
Beerend P. Hierck
Keyword(s):  
Shear Stress ◽  
Primary Cilia ◽  
Fluid Shear Stress ◽  
Vascular System ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial ◽  
Fluid Shear ◽  
Cellular Functions ◽  
Continuous Problem

Local regulation of vascular tone plays an important role in cardiovascular control of blood pressure. Aside from chemical or hormonal regulations, this local homeostasis is highly regulated by fluid-shear stress. It was previously unclear how vascular endothelial cells were able to sense fluid-shear stress. The cellular functions of mechanosensory cilia within vascular system have emerged recently. In particular, hypertension is insidious and remains a continuous problem that evolves during the course of polycystic kidney disease (PKD). The basic and clinical perspectives on primary cilia are discussed with regard to the pathogenesis of hypertension in PKD.

Role of cadherins and plakoglobin in interendothelial adhesion under resting conditions and shear stress

1997 ◽  
Vol 273 (5) ◽  
pp. H2396-H2405 ◽  
Author(s):  
Hans-Joachim Schnittler ◽  
Bernd Püschel ◽  
Detlev Drenckhahn
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Time Course ◽  
Fluid Shear Stress ◽  
Vascular Endothelial ◽  
Fluid Shear ◽  
Platelet Endothelial Cell Adhesion ◽  
Cell Adhesion Molecule 1 ◽  
Arterial Endothelial Cells

The role of cadherins and the cadherin-binding cytosolic protein plakoglobin in intercellular adhesion was studied in cultured human umbilical venous endothelial cells exposed to fluid shear stress. Extracellular Ca2+depletion (<10−7 M) caused the disappearance of both cadherins and plakoglobin from junctions, whereas the distribution of platelet endothelial cell adhesion molecule 1 (PECAM-1) remained unchanged. Cells stayed fully attached to each other for several hours in low Ca2+ but began to dissociate under flow conditions. At the time of recalcification, vascular endothelial (VE) cadherin and β-catenin became first visible at junctions, followed by plakoglobin with a delay of ∼20 min. Full fluid shear stress stability of the junctions correlated with the time course of the reappearance of plakoglobin. Inhibition of plakoglobin expression by microinjection of antisense oligonucleotides did not interfere with the junctional association of VE-cadherin, PECAM-1, and β-catenin. The plakoglobin-deficient cells remained fully attached to each other under resting conditions but began to dissociate in response to flow. Shear stress-induced junctional dissociation was also observed in cultures of plakoglobin-depleted arterial endothelial cells of the porcine pulmonary trunk. These observations show that interendothelial adhesion under hydrodynamic but not resting conditions requires the junctional location of cadherins associated with plakoglobin. β-Catenin cannot functionally compensate for the junctional loss of plakoglobin, and PECAM-1-mediated adhesion is not sufficient for monolayer integrity under flow.

A device for subjecting vascular endothelial cells to both fluid shear stress and circumferential cyclic stretch

1994 ◽  
Vol 22 (4) ◽  
pp. 416-422 ◽  
Author(s):  
James E. Moore ◽  
Ernst Bürki ◽  
Andreas Suciu ◽  
Shumin Zhao ◽  
Michel Burnier ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Fluid Shear Stress ◽  
Vascular Endothelial Cells ◽  
Cyclic Stretch ◽  
Vascular Endothelial ◽  
Fluid Shear

scholarly journals Fluid Shear Stress Induces Lipocalin-Type Prostaglandin D 2 Synthase Expression in Vascular Endothelial Cells

2000 ◽  
Vol 86 (9) ◽  
pp. 967-973 ◽  
Author(s):  
Yoji Taba ◽  
Toshiyuki Sasaguri ◽  
Megumi Miyagi ◽  
Takeo Abumiya ◽  
Yoshikazu Miwa ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Fluid Shear Stress ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial ◽  
Fluid Shear
Sign in / Sign up

Export Citation Format

Share Document