scholarly journals Bioseparation of Four Proteins fromEuphorbia characiasLatex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid Phosphatase

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Rosaria Medda ◽  
Francesca Pintus ◽  
Delia Spanò ◽  
Giovanni Floris
Keyword(s):  
Acid Phosphatase ◽  
Amine Oxidase ◽  
Deae Cellulose ◽  
Purple Acid Phosphatase ◽  
Copper Amine Oxidase ◽  
Nucleotide Pyrophosphatase ◽  
Euphorbia Characias ◽  
High Purification ◽  
Purification Methods ◽  
Different Sources

This paper deals with the purification of four proteins fromEuphorbia characiaslatex, a copper amine oxidase, a nucleotide pyrophosphatase/phosphodiesterase, a peroxidase, and a purple acid phosphatase. These proteins, very different in molecular weight, in primary structure, and in the catalyzed reaction, are purified using identical preliminary steps of purification and by chromatographic methods. In particular, the DEAE-cellulose chromatography is used as a useful purification step for all the four enzymes. The purification methods here reported allow to obtain a high purification of all the four proteins with a good yield. This paper will give some thorough suggestions for researchers busy in separation of macromolecules from different sources.

scholarly journals DEER and RIDME Measurements of the Nitroxide-Spin Labelled Copper-Bound Amine Oxidase Homodimer from Arthrobacter Globiformis

2021 ◽  
Author(s):  
Hannah Russell ◽  
Rachel Stewart ◽  
Christopher Prior ◽  
Vasily S. Oganesyan ◽  
Thembaninkosi G. Gaule ◽  
...  
Keyword(s):  
Dipolar Coupling ◽  
Amine Oxidase ◽  
Spin Labels ◽  
Arthrobacter Globiformis ◽  
Copper Amine Oxidase ◽  
Dipole Interactions ◽  
Nitroxide Spin Labels ◽  
Double Electron Electron Resonance ◽  
Dipolar Modulation ◽  
Paramagnetic Centres

AbstractIn the study of biological structures, pulse dipolar spectroscopy (PDS) is used to elucidate spin–spin distances at nanometre-scale by measuring dipole–dipole interactions between paramagnetic centres. The PDS methods of Double Electron Electron Resonance (DEER) and Relaxation Induced Dipolar Modulation Enhancement (RIDME) are employed, and their results compared, for the measurement of the dipolar coupling between nitroxide spin labels and copper-II (Cu(II)) paramagnetic centres within the copper amine oxidase from Arthrobacter globiformis (AGAO). The distance distribution results obtained indicate that two distinct distances can be measured, with the longer of these at c.a. 5 nm. Conditions for optimising the RIDME experiment such that it may outperform DEER for these long distances are discussed. Modelling methods are used to show that the distances obtained after data analysis are consistent with the structure of AGAO.

scholarly journals Time-resolved analysis of catalytic reaction of copper amine oxidase fromArthrobacter globiformis

2011 ◽  
Vol 67 (a1) ◽  
pp. C225-C225
Author(s):  
H. Yamaguchi ◽  
M. Kataoka ◽  
H. Oya ◽  
A. Tominaga ◽  
M. Ohtsu ◽  
...  
Keyword(s):  
Catalytic Reaction ◽  
Amine Oxidase ◽  
Copper Amine Oxidase ◽  
Time Resolved ◽  
Copper Amine

Molecular cloning and characterization of copper amine oxidase from Huperzia serrata

2012 ◽  
Vol 22 (18) ◽  
pp. 5784-5790 ◽  
Author(s):  
Jieyin Sun ◽  
Hiroyuki Morita ◽  
Guoshen Chen ◽  
Hiroshi Noguchi ◽  
Ikuro Abe
Keyword(s):  
Molecular Cloning ◽  
Amine Oxidase ◽  
Copper Amine Oxidase ◽  
Huperzia Serrata ◽  
Copper Amine

Synthesis, Structural, Magnetic, and Redox Properties of Asymmetric Diiron Complexes with a Single Terminally Bound Phenolate Ligand. Relevance to the Purple Acid Phosphatase Enzymes

1997 ◽  
Vol 119 (40) ◽  
pp. 9424-9437 ◽  
Author(s):  
Elisabeth Lambert ◽  
Barbara Chabut ◽  
Sylvie Chardon-Noblat ◽  
Alain Deronzier ◽  
Geneviève Chottard ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Redox Properties ◽  
Purple Acid Phosphatase

scholarly journals The Soybean Purple Acid Phosphatase GmPAP14 Predominantly Enhances External Phytate Utilization in Plants

2018 ◽  
Vol 9 ◽  
Author(s):  
Youbin Kong ◽  
Xihuan Li ◽  
Bing Wang ◽  
Wenlong Li ◽  
Hui Du ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Purple Acid Phosphatase

scholarly journals Identification of Purple Acid Phosphatase Inhibitors by Fragment-Based Screening: Promising New Leads for Osteoporosis Therapeutics

2012 ◽  
Vol 80 (5) ◽  
pp. 665-674 ◽  
Author(s):  
Daniel Feder ◽  
Waleed M. Hussein ◽  
Daniel J. Clayton ◽  
Meng-Wei Kan ◽  
Gerhard Schenk ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Purple Acid Phosphatase ◽  
Phosphatase Inhibitors

scholarly journals The active site of purple acid phosphatase from sweet potatoes (Ipomoea batatas) . Metal content and spectroscopic characterization

1999 ◽  
Vol 260 (3) ◽  
pp. 709-716 ◽  
Author(s):  
Atila Durmus ◽  
Christoph Eicken ◽  
Bernd Horst Sift ◽  
Andreas Kratel ◽  
Reinhard Kappl ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Active Site ◽  
Metal Content ◽  
Ipomoea Batatas ◽  
Spectroscopic Characterization ◽  
Purple Acid Phosphatase ◽  
Sweet Potatoes

scholarly journals Unique protonation states of aspartate and topaquinone in the active site of copper amine oxidase

RSC Advances ◽  
2020 ◽  
Vol 10 (63) ◽  
pp. 38631-38639
Author(s):  
Mitsuo Shoji ◽  
Takeshi Murakawa ◽  
Mauro Boero ◽  
Yasuteru Shigeta ◽  
Hideyuki Hayashi ◽  
...  
Keyword(s):  
Biogenic Amines ◽  
Active Site ◽  
Amine Oxidase ◽  
First Principle ◽  
Amine Oxidases ◽  
Oxidative Deamination ◽  
Copper Amine Oxidase ◽  
First Principle Calculations ◽  
Copper Amine Oxidases ◽  
Copper Amine

Copper amine oxidases catalyze the oxidative deamination of biogenic amines. We investigated the unique protonation states in the active site using first-principle calculations.

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