scholarly journals Biosurfactant-Producing Lactobacilli: Screening, Production Profiles, and Effect of Medium Composition

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Eduardo J. Gudiña ◽  
José A. Teixeira ◽  
Lígia R. Rodrigues
Keyword(s):  
Surface Tension ◽  
Optimization Procedure ◽  
Nitrogen Sources ◽  
Culture Broth ◽  
Medium Composition ◽  
Biosurfactant Production ◽  
Standard Medium ◽  
Meat Extract ◽  
Surface Tension Reduction ◽  
Dairy Plant

Biosurfactant production was screened in four lactobacilli strains. The highest biosurfactant production (excreted and cell-bound biosurfactants) was achieved withLactobacillus paracaseissp.paracaseiA20, a strain isolated from a Portuguese dairy plant, with a decrease in the surface tension of 6.4 mN m−1and 22.0 mN m−1, respectively. Biosurfactant production by this strain was evaluated under different culture broth compositions. The use of different nitrogen sources revealed that yeast extract is essential for bacterial growth, while peptone is crucial for biosurfactant synthesis. For biosurfactant production, the use of peptone and meat extract yielded a higher production when compared to the standard medium, with a surface tension reduction of 24.5 mN m−1Furthermore, experiments were also conducted in a reactor with pH and temperature control. Biomass and biosurfactant production in bioreactor was higher comparing with the experiments conducted in shake flaks. The optimization procedure adopted in the current work was found to improve the biosurfactant production and opened new perspectives for the use ofL. paracaseissp.paracaseiA20 as a promising biosurfactant-producer.

scholarly journals Factorial Design to Optimize Biosurfactant Production byYarrowia lipolytica

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
Gizele Cardoso Fontes ◽  
Priscilla Filomena Fonseca Amaral ◽  
Marcio Nele ◽  
Maria Alice Zarur Coelho
Keyword(s):  
Surface Tension ◽  
Factorial Design ◽  
Ammonium Sulfate ◽  
Yeast Extract ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Biosurfactant Production ◽  
Standard Process ◽  
Emulsification Index ◽  
Full Factorial

In order to improve biosurfactant production byYarrowia lipolyticaIMUFRJ 50682, a factorial design was carried out. A24full factorial design was used to investigate the effects of nitrogen sources (urea, ammonium sulfate, yeast extract, and peptone) on maximum variation of surface tension (ΔST) and emulsification index (EI). The best results (67.7% of EI and 20.9 mNm−1ofΔST) were obtained in a medium composed of 10 g 1−1of ammonium sulfate and 0.5 g 1−1of yeast extract. Then, the effects of carbon sources (glycerol, hexadecane, olive oil, and glucose) were evaluated. The most favorable medium for biosurfactant production was composed of both glucose (4% w/v) and glycerol (2% w/v), which provided an EI of 81.3% and aΔST of 19.5 mN m−1. The experimental design optimization enhancedΔEI by 110.7% andΔST by 108.1% in relation to the standard process.

scholarly journals The effect of medium composition on the production of sophorolipids and the tensiometric properties by Starmerella bombicola MTCC 1910

2010 ◽  
Vol 12 (2) ◽  
pp. 9-13 ◽  
Author(s):  
Nagarajan Vedaraman ◽  
Narayana Venkatesh
Keyword(s):  
Surface Tension ◽  
Yeast Extract ◽  
Sunflower Oil ◽  
Free Cell ◽  
Medium Composition ◽  
Candida Bombicola ◽  
Standard Medium ◽  
Substrate Composition ◽  
Starmerella Bombicola ◽  
Minimum Surface Tension

The effect of medium composition on the production of sophorolipids and the tensiometric properties by Starmerella bombicola MTCC 1910 Starmerella bombicola a teleomorph of Candida bombicola is capable of producing extracellular secondary metabolites known as sophorolipids. In the present work the performance of Starmerella in producing sophorolipids, with standard medium ingredients glucose, sunflower oil, yeast extract and urea was studied. The quantities of different medium ingredients were optimized to maximize the production of sophorolipids. Variation in tensiometric properties like surface tension and interfacial tension during the incubation period were also reported. The optimized mixed substrate composition was found to be 200 g/l, containing equal amounts of glucose and sunflower oil, 4 g/l of yeast extract and 0.6 g/l of urea. With the optimized substrate composition 38.6 g/l of sophorolipids was obtained. The minimum surface tension produced by the culture free cell broth was 36.2 mN/m. Increasing the temperature from 25°C to 35°C has shown adverse effects on sophorolipids production.

scholarly journals Biosurfactant production by Mucor circinelloides on waste frying oil and possible uses in crude oil remediation

2017 ◽  
Vol 76 (7) ◽  
pp. 1706-1714 ◽  
Author(s):  
Parvin Hasanizadeh ◽  
Hamid Moghimi ◽  
Javad Hamedi
Keyword(s):  
Surface Tension ◽  
Optimum Condition ◽  
Crude Oil ◽  
Fungal Growth ◽  
Mucor Circinelloides ◽  
Frying Oil ◽  
Biosurfactant Production ◽  
Waste Frying Oil ◽  
Tension Reduction ◽  
Surface Tension Reduction

Biosurfactants are biocompatible surface active agents which many microorganisms produce. This study investigated the production of biosurfactants by Mucor circinelloides. The effects of different factors on biosurfactant production, including carbon sources and concentrations, nitrogen sources, and iron (II) concentration, were studied and the optimum condition determined. Finally, the strain's ability to remove the crude oil and its relationship with biosurfactant production was evaluated. The results showed that M. circinelloides could reduce the surface tension of the culture medium to 26.6 mN/m and create a clear zone of 12.9 cm diameter in an oil-spreading test. The maximum surface tension reduction was recorded 3 days after incubation. The optimum condition for biosurfactant production was achieved in the presence of 8% waste frying oil as a carbon source, 2 g/L yeast extract as a nitrogen source, and 0.01 mM FeSO4. M. circinelloides could consume 8% waste frying oil in 5 days of incubation, and 87.6% crude oil in 12 days of incubation. A direct correlation was observed between oil degradation and surface tension reduction in the first 3 days of fungal growth. The results showed that the waste frying oil could be recommended as an inexpensive oily waste substance for biosurfactant production, and M. circinelloides could have the potential to treat waste frying oil. According to the results, the produced crude biosurfactant or fungal strain could be directly used for the mycoremediation of crude oil contamination in oil fields.

Gordonia (nocardia) amarae foaming due to biosurfactant production

2002 ◽  
Vol 46 (1-2) ◽  
pp. 519-524 ◽  
Author(s):  
K.R. Pagilla ◽  
A. Sood ◽  
H. Kim
Keyword(s):  
Surface Tension ◽  
Carbon Source ◽  
Stationary Phase ◽  
Activated Sludge ◽  
Sole Carbon Source ◽  
Wastewater Treatment Plants ◽  
Carbon Sources ◽  
Biomass Concentration ◽  
Culture Broth ◽  
Biosurfactant Production

Gordonia amarae, a filamentous actinomycete, commonly found in foaming activated sludge wastewater treatment plants was investigated for its biosurfactant production capability. Soluble acetate and sparingly soluble hexadecane were used as carbon sources for G. amarae growth and biosurfactant production in laboratory scale batch reactors. The lowest surface tension (critical micelle concentration, CMC) of the cell-free culture broth was 55 dynes/cm when 1,900 mg/L acetate was used as the sole carbon source. The lowest surface tension was less than 40 dynes/cm when either 1% (v/v) hexadecane or a mixture of 1% (v/v) hexadecane and 0.5% (w/v) acetate was used as the carbon source. The maximum biomass concentration (the stationary phase) was achieved after 4 days when acetate was used along with hexadecane, whereas it took about 8 days to achieve the stationary phase with hexadecane alone. The maximum biosurfactant production was 3 × CMC with hexadecane as the sole carbon source, and it was 5 × CMC with the mixture of hexadecane and acetate. Longer term growth studies (∼ 35 days of culture growth) indicated that G. amarae produces biosurfactant in order to solubilize hexadecane, and that adding acetate improves its biosurfactant production by providing readily degradable substrate for initial biomass growth. This research confirms that the foaming problems in activated sludge containing G. amarae in the activated sludge are due to the biosurfactant production by G. amarae when hydrophobic substrates such as hexadecane are present.

scholarly journals Production of biosurfactant by hydrocarbon degrading Rhodococcus ruber and Rhodococcus erythropolis

1999 ◽  
Vol 30 (3) ◽  
pp. 231-236 ◽  
Author(s):  
Flávio Correa Bicca ◽  
Leonardo Colombo Fleck ◽  
Marco Antônio Záchia Ayub
Keyword(s):  
Surface Tension ◽  
Oil Spills ◽  
Hydrophobic Interactions ◽  
Rhodococcus Erythropolis ◽  
Medium Composition ◽  
Biosurfactant Production ◽  
Rhodococcus Ruber ◽  
Biotechnological Production ◽  
Reduce Surface Tension ◽  
Production Strain

There is world wide concern about the liberation of hydrocarbons in the environment, both from industrial activities and from accidental spills of oil and oilrelated compounds. Biosurfactants, which are natural emulsifiers of hydrocarbons, are produced by some bacteria, fungi and yeast. They are polymers, totally or partially extracellular, with an amphipathyc structure, which allows them to form micelles that accumulate at the interface between liquids of different polarities such as water and oil. This process is based upon the ability of biosurfactants to reduce surface tension, blocking the formation of hydrogen bridges and certain hydrophilic and hydrophobic interactions. The ability of biosurfactant production by five strains of Rhodococcus isolated from oil prospecting sites was evaluated. Surface tension measurement and emulsifying index were used to quantify biosurfactant production. The influence of environmental conditions was also investigated - pH, temperature, medium composition, and type of carbon source - on cell growth and biosurfactant production. Strain AC 239 was shown to be a potential producer, attaining 63% of emulsifying index for a Diesel-water binary system. It could be used, either directly on oil spills in contained environments, or for the biotechnological production of biosurfactant.

scholarly journals The potential of indigenous bacteria from oil sludge for biosurfactant production using hydrolysate of agricultural waste

2019 ◽  
Vol 20 (5) ◽  
Author(s):  
NI’MATUZAHROH NI’MATUZAHROH ◽  
SILVIA KURNIA SARI ◽  
IRINE PUSPA NINGRUM ◽  
APRILLA DILA PUSFITA ◽  
LISA MARJAYANDARI ◽  
...  
Keyword(s):  
Surface Tension ◽  
Raw Materials ◽  
Agricultural Waste ◽  
Hydrolysis Product ◽  
Biosurfactant Production ◽  
Oil Sludge ◽  
Plate Count ◽  
Indigenous Bacteria ◽  
Emulsification Activity ◽  
Surface Tension Reduction

Abstract. Ni’matuzahroh, Sari SK, Ningrum IP, Pusfita AD, Marjayandari L, Trikurniadewi N, Ibrahim SNMM, Fatimah, Nurhariyati T, Surtiningsih T, Yuliani H. 2019. The potential of indigenous bacteria from oil sludge for biosurfactant production using hydrolysate of agricultural waste. Biodiversitas 20: 1374-1379. Biosurfactants are amphipathic compounds which are useful in various fields of health, industry, and remediation. Biosurfactants are produced by bacteria that grow in hydrocarbon or sugar substrates. Hydrolysis product of agricultural waste can be used as a biosurfactant production medium. This research aims to obtain biosurfactant producing bacteria from Balongan oil sludge, Indonesia. The ability to grow and produce biosurfactant by indigenous bacteria was tested using a medium of Synthetic Mineral Water (SMW) added by 209.3 ppm of rice straw hydrolysis product (RSHP). The growth of bacteria was evaluated through Total Plate Count (TPC) and biosurfactant production was evaluated through measurement of emulsification activity and surface tension. Six indigenous bacteria were capable to produce biosurfactants in the RSHP. Emulsification activity was not detected, but surface tension reduction was founded. The best biosurfactant was indicated by surface tension value of 53.56 mN/m with TPC value of 20.07 CFU/mL at the 5th day of incubation by BP (1) 5. The indigenous bacteria were identified as Propionibacterium BP (1) 1, Propionibacterium BP (1) 3, Bacillus BP (1) 4, Corynebacterium BP (1) 5, Corynebacterium BP (1) 8, and Rothia BP (1) 6. Utilization of sugar as hydrolysis product of agricultural waste is an innovation of raw materials for biosurfactant production.

scholarly journals Production of Biosurfactant in 2L Bioreactor Using Sludge Palm Oil as a Substrate

1970 ◽  
Vol 12 (4) ◽  
Author(s):  
P. JAMAL ◽  
Md Zahangir Alam ◽  
E. A. Zainuddin ◽  
And W. M. F. W. Nawawi
Keyword(s):  
Surface Tension ◽  
Factorial Design ◽  
Process Optimization ◽  
Palm Oil ◽  
Biosurfactant Production ◽  
Raw Material ◽  
Percentage Error ◽  
Meat Extract ◽  
Design Expert ◽  
Sludge Palm Oil

In this study, sludge palm oil was utilized as a raw material for process optimization in a stirred tank bioreactor for the biosurfactant production. Optimized parameters include temperature, agitation and aeration while the pH and media compositions such as NaNO3, NaCl, FeSO4, Meat extract, and glucose have been fixed from the previous study. The design of this research was made by using the Design-Expert software (2 level factorial design). Surface tension was used as indirect indicator for biosurfactant production. After model validation, yield of biosurfactant was found to be highest when surface tension was at its lowest value (<24 mN/m) at temperature of 300C , agitation 300 rpm and around 0.5 vvm of aeration with percentage error less than 10% between observed value and predicted value. The finding in this research can be applied to produce biosurfactant in large amount from the less expensive material such as sludge palm oil by using the same optimized model equation.ABSTRAK: Dalam kajian ini, enapcemar kelapa sawit digunakan sebagai bahan mentah untuk pengoptimuman proses dalam bioreaktor bagi penghasilan biosurfactant. Parameter yang dioptimumkan termasuk suhu, pergolakan dan pengudaraan manakala pH dan komposisi media seperti NaNO3, NaCl, FeSO4, ekstrak daging, dan glukos telah ditetapkan daripada kajian sebelumnya. Reka bentuk kajian ini telah dilakukan dengan menggunakan perisian Design Expert (2 Level factorial design). Tegangan permukaan digunakan sebagai penunjuk tidak langsung untuk penghasilan biosurfactant. Selepas pengesahan, hasil biosurfactant didapati terbanyak apabila tegangan permukaan pada nilai terendah (<24 mN/m) pada suhu 300C, pergolakan 300 rpm, dan sekitar 0.5 vvm pengudaraan dengan margin perbezaan kurang daripada 10% antara nilai sebenar cerapan dan nilai yang diramalkan. Penemuan dalam kajian ini boleh digunakan untuk menghasilkan biosurfactant dalam jumlah yang besar dari bahan yang murah seperti enapcemar kelapa sawit dengan menggunakan model optimum yang sama.KEY WORDS:  Biosurfactant, Sludge Palm Oil, Process optimization, and Factorial design.

scholarly journals Effects of Nitrogen and Carbon Sources on Biosurfactant Production by Hydrocarbon-utilizing Stenotrophomonas sp.

2019 ◽  
pp. 1-10
Author(s):  
Victor Ezebuiro ◽  
Ipeghan Jonathan Otaraku ◽  
Boma Oruwari ◽  
Gideon Chijioke Okpokwasili
Keyword(s):  
Surface Tension ◽  
Olive Oil ◽  
Yeast Extract ◽  
Emulsion Stability ◽  
Carbon Sources ◽  
Glass Slide ◽  
Biosurfactant Production ◽  
Tension Reduction ◽  
Emulsification Index ◽  
Surface Tension Reduction

Aim: This study investigated effects of nitrogen and carbon sources on the production of biosurfactant by a hydrocarbon-utilizing bacterium, Stenotrophomonas sp. Methodology: The hydrocarbon-utilizing bacterium was isolated with Bushnell Haas (BH) broth using enrichment method. Biosurfactant production was screened by evaluating the following characteristics: Emulsification index (E-24), oil spreading (displacement), tilted glass slide, haemolysis on blood agar, and lipase production. Effects of combination of nitrogen sources (yeast extract and NH4NO3, yeast extract and urea, yeast extract and asparagine, yeast extract and peptone, NaNO3 and peptone, NaNO3 and asparagine, and yeast extract and NaNO3) and carbon sources (glucose, fructose, galactose, cassava peel, soya bran, olive oil, sucrose, crude oil, diesel and glycerol) on biosurfactant production were determined with emulsion stability and surface tension as responses. The bacterium was identified based on phenotypic, microscopic, and biochemical characteristics. Results: The isolate produced colonies on BH agar containing either naphthalene or hexadecane as sole source of carbon after 48-h incubation. Screening characteristics for the production of biosurfactant by the isolate were as follows: 46% emulsification index, 3.1 cm2 oil displacement, 1.8 cm zone of clearance on tributyrin agar, γ-haemolysis, and positive tilted glass slide. The best carbon source with the highest emulsion stability (51.6%) was fructose whereas the best surface tension reduction (30.85 mN/m) was observed with olive oil as carbon sources after 7 days of incubation. For nitrogen, the combination of yeast extract and NH4NO3 gave the highest emulsion stability (60.7%) and the best surface tension reduction (39.58 mN/m). The data obtained were significant at P<0.05 and the bacterial isolate identified as Stenotrophomonas sp. Conclusion: This study has demonstrated the ability of the hydrocarbon-utilizing bacterium, Stenotrophomonas sp. to produce biosurfactant, indicated by reduction of surface tension and formation of stable emulsion. This method of biosurfactant production can be further scaled up for industrial purpose. 

scholarly journals UJI PRODUKSI BIOSURFAKTAN OLEH Pseudomonas sp. PADA SUBSTRAT YANG BERBEDA

2007 ◽  
Vol 12 (2) ◽  
pp. 181-185 ◽  
Author(s):  
Fatimah Fatimah
Keyword(s):  
Surface Tension ◽  
Stationary Phase ◽  
Yeast Extract ◽  
Petroleum Hydrocarbon ◽  
Biosurfactant Production ◽  
Lubricating Oil ◽  
Pseudomonas Sp ◽  
Emulsification Activity ◽  
Microbial Metabolite ◽  
Surface Tension Reduction

Biosurfactant, microbial metabolite whose properties like surfactant, was suggested to replace chemically synthesized surfactant for take in hand environtmental pollution by petroleum hydrocarbon. This work was done to examine potency of Pseudomonas sp. isolated from Tanjung Perak Harbor to produce biosurfactant. Also, to know the effect of different substrates (glucose plus yeast extract, lubricating oil and hexadecane) toward biosurfactant production. Pseudomonas sp. grown in mineral synthetic water and biosurfactant production was measured on stationary phase. Biosurfactant production based on emulsification activity and surface tension reduction of supernatant (using Du Nouy tensiometer). Solar, lubricating oil, and hexadecane were used to examine emulsification activity. Results indicated that Pseudomonas sp. have a potency to produce biosurfactant. Surface tension of supernatant decreased up to 20 dyne/cm, when grown on hexadecane substrate. Hexadecane is the best growing substrate for biosurfactant production than others.

Biosurfactant Production Using Mixed Cultures Under Non-Aseptic Conditions

1994 ◽  
Vol 344 ◽  
Author(s):  
C. Vipulanandan ◽  
G. L. Ghurye ◽  
R. C. Willson
Keyword(s):  
Surface Tension ◽  
Yeast Extract ◽  
Substrate Concentration ◽  
Petroleum Hydrocarbons ◽  
Biomass Accumulation ◽  
Biosurfactant Production ◽  
Batch Reactors ◽  
Surface Tension Reduction ◽  
Aseptic Conditions ◽  
Reduced Surface

AbstractSurfactants increase the accessibility of adsorbed hydrocarbons and mobilize immiscible petroleum hydrocarbons for treatment. Biosurfactants have the advantage of biodegradability and non-toxicity over their synthetic counterparts, and can be produced from renewable sources. In this study the production of biosurfactant from molasses was investigated in continuously stirred batch reactors. The effects of substrate concentration, yeast extract and peptone on biomass accumulation and biosurfactant production were investigated. Biosurfactant production was quantified by surface tension reduction and critical micelle dilution (CMD). Biosurfactant production was directly correlated with biomass production, and was improved with the addition of yeast extract. Centrifugation of the whole broth reduced surface tension. The performance of the biosurfactant produced from molasses under non-aseptic condition is comparable to other published results.

Sign in / Sign up

Export Citation Format

Share Document