scholarly journals Differential Gene Expression in Sugarcane in Response to Challenge by Fungal PathogenUstilago scitamineaRevealed by cDNA-AFLP

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Que You-Xiong ◽  
Lin Jian-Wei ◽  
Song Xian-Xian ◽  
Xu Li-Ping ◽  
Chen Ru-Kai
Keyword(s):  
Gene Expression ◽  
Differential Gene Expression ◽  
Silver Staining ◽  
Molecular Basis ◽  
Pcr Analysis ◽  
Aflp Analysis ◽  
Rt Pcr ◽  
Resistant Genotype ◽  
Ustilago Scitaminea ◽  
Differential Gene

Differential gene expression in sugarcane during sugarcane-Ustilago scitamineainteraction was conducted in a smut-resistant genotype. Using cDNA-AFLP along with silver staining, a total of 136 transcript-derived fragments (TDFs) were found to be differentially expressed in response to challenge byU. scitaminea. Forty TDFs, 34 newly induced plus six with obvious upregulated expression after infection, were sequenced and validated by RT-PCR analysis. These results demonstrated that the expression of 37 out of these TDFs in RT-PCR analysis was consistent with that in cDNA-AFLP analysis. Based on BlastX in NCBI, 28 TDFs were assumed to function in sugarcane underU. scitamineastress. Analysis of expression profile of three TDFs revealed that they responded differently after infection withU. scitaminea, and the transcription was significantly enhanced. The response of two TDFs, SUC06 and SUC09, occurred before that of SUC10. This study enriches our knowledge of the molecular basis for sugarcane response toU. scitamineainfection.

cDNA-AFLP Analysis Reveals Differential Gene Expression in Wheat Adult- Plant Resistance to Stripe Rust

2010 ◽  
Vol 36 (3) ◽  
pp. 401-409 ◽  
Author(s):  
Gang ZHANG ◽  
Yan-Ling DONG ◽  
Ning XIA ◽  
Yi ZHANG ◽  
Xiao-Jie WANG ◽  
...  
Keyword(s):  
Gene Expression ◽  
Differential Gene Expression ◽  
Stripe Rust ◽  
Plant Resistance ◽  
Adult Plant Resistance ◽  
Adult Plant ◽  
Aflp Analysis ◽  
Differential Gene

Differential Gene Expression During Somatic Embryogenesis in Coffea arabica L., Revealed by RT-PCR Differential Display

2002 ◽  
Vol 21 (1) ◽  
pp. 043-050 ◽  
Author(s):  
R Rojas-Herrera ◽  
F Quiroz-Figueroa ◽  
M Monforte-González ◽  
L Sánchez-Teyer ◽  
V M Loyola-Vargas
Keyword(s):  
Gene Expression ◽  
Somatic Embryogenesis ◽  
Differential Gene Expression ◽  
Differential Display ◽  
Coffea Arabica ◽  
Rt Pcr ◽  
Differential Gene ◽  
Coffea Arabica L

scholarly journals Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: Analysis of gene expression during potato tuber development

1996 ◽  
Vol 9 (5) ◽  
pp. 745-753 ◽  
Author(s):  
Christian W.B. Bachem ◽  
Rutger S. van der Hoeven ◽  
Steef M. de Bruijn ◽  
Dick Vreugdenhil ◽  
Marc Zabeau ◽  
...  
Keyword(s):  
Gene Expression ◽  
Potato Tuber ◽  
Differential Gene Expression ◽  
Aflp Analysis ◽  
Tuber Development ◽  
Novel Method ◽  
Differential Gene ◽  
Rna Fingerprinting

cDNA-AFLP analysis of differential gene expression during the interaction between sugarcane and Puccinia melanocephala

2004 ◽  
Vol 123 (5) ◽  
pp. 499-501 ◽  
Author(s):  
E. Carmona ◽  
D. Vargas ◽  
C. J. Borroto ◽  
J. Lopez ◽  
A. I. Fernandez ◽  
...  
Keyword(s):  
Gene Expression ◽  
Differential Gene Expression ◽  
Aflp Analysis ◽  
Puccinia Melanocephala ◽  
Differential Gene
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