scholarly journals Studies on Fungal Cultural Filtrates against AdultCulex quinquefasciatus(Diptera: Culicidae) a Vector of Filariasis

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Gavendra Singh ◽  
Soam Prakash
Keyword(s):  
Fusarium Oxysporum ◽  
Entomopathogenic Fungi ◽  
Filter Paper ◽  
Mosquito Population ◽  
Fungal Metabolites ◽  
Lagenidium Giganteum ◽  
Significant Potential ◽  
Culture Filtrates

Entomopathogenic fungi have significant potential to control mosquito population. The culture filtrates ofFusarium oxysporum, Lagenidium giganteum, Trichophyton ajelloi,andCulicinomyces clavisporuswere evaluated against adults ofCx. quinquefasciatus. The culture filtrates were obtained by filtering the broth through Whatman-1 filter paper. These culture filtrates ofC. clavisporushave been found significantly pathogenic with LC50-2.5, LC90-7.24, and LC99-8.7 ML, respectively, after exposure of 24 h. However, the culture filtrates when were combined, in ratios 1 : 1 : 1 ofFusarium oxysporum, Lagenidium giganteum, Trichophyton ajelloithe mortalities were significantly increased. The LC50-3.71, LC90-8.12, and LC99-11.48 were significantly recorded after exposure of 10 hrs. Similarly, the culture filtrates ofT. ajelloi, Culicinomyces clavisporus,andL. giganteumhave been combined in ratios 1 : 1 : 1. Similarly the LC50-1.94, LC90-4, and LC99-6.16 ML Were recorded after exposure of 10 hrs. The results of present study show promise for the use of selected fungal metabolites for control ofCx. quinquefasciatusin the Laboratory.

Effect of Buffers on the Pectolytic Activity of Culture Filtrates of Fusarium oxysporum

Nature ◽  
1964 ◽  
Vol 202 (4930) ◽  
pp. 414-415 ◽  
Author(s):  
J. A. MEYER ◽  
E. D. GARBER ◽  
SUSAN G. SHAEFFER
Keyword(s):  
Fusarium Oxysporum ◽  
Culture Filtrates ◽  
Pectolytic Activity

In vitro phytotoxicity of culture filtrates of Fusarium oxysporum f. sp. vanillae in Vanilla planifolia Jacks

2015 ◽  
Vol 197 ◽  
pp. 573-578 ◽  
Author(s):  
Marco Antonio Ramírez-Mosqueda ◽  
Lourdes Georgina Iglesias-Andreu ◽  
Mauricio Luna-Rodríguez ◽  
Alejandro Antonio Castro-Luna
Keyword(s):  
Fusarium Oxysporum ◽  
Vanilla Planifolia ◽  
Culture Filtrates

The 24-kDa protein from Fusarium oxysporum f.sp. erythroxyli: occurrence in related fungi and the effect of growth medium on its production

1997 ◽  
Vol 43 (1) ◽  
pp. 45-55 ◽  
Author(s):  
Bryan A. Bailey ◽  
James C. Jennings ◽  
James D. Anderson
Keyword(s):  
Fusarium Oxysporum ◽  
Protein Production ◽  
Fusarium Species ◽  
Infected Plant ◽  
Water Soluble ◽  
Western Blots ◽  
Fungal Isolates ◽  
Culture Filtrates ◽  
Dicotyledonous Plants ◽  
Casamino Acids

A 24-kDa protein that elicits ethylene production and necrosis in leaves of dicotyledonous plants was previously purified from culture filtrates of Fusarium oxysporum Schlechtend:Fr. f.sp. erythroxyli. Antisera to the denatured 24-kDa protein detected 2.5 ng of the 24-kDa protein on Western blots at 100 000-fold dilutions. The antisera cross-reacted with a 24-kDa protein on Western blots of culture filtrates from three other F. oxysporum formae speciales. Of seven Fusarium species, only F. oxysporum, F. acuminatum Ellis and Kellerm., and F. avenaceum (Fr.:Fr.) Sacc. isolates produced an antigenically related 24-kDa protein. Although there were differences in the profiles of proteins extracted from stems of coca (Erythroxylum coca var. coca L. Lam.) infected with F. oxysporum f.sp. erythroxyli compared with uninfected stems, antisera to the 24-kDa protein did not cross-react with any proteins from the infected coca stems. For the fungal isolates studied, the best medium tested for production of the 24-kDa protein contained 1% sucrose and 1% asparagine. Biological activity of the F. oxysporum culture filtrates on sweet basil leaves was consistently correlated with the presence of the 24-kDa protein. Production of the 24-kDa protein was limited in cultures containing pectin or cellulose as the primary carbon source, or in cultures lacking sucrose or casamino acids. Water-soluble extracts from coca stems inhibited production of the 24-kDa protein, whereas cellulose and pectin did not. Components produced by the plant may limit production of the 24-kDa protein in infected plant tissue and thereby limit the response of the plant to the fungus. These results suggest the 24-kDa protein does not function in the symptomatic phase of the F. oxysporum f.sp. erythroxyli–coca disease interaction.Key words: Fusarium oxysporum, toxin, elicitor.

In vitro antagonism of endophytic Fusarium oxysporum isolates against the burrowing nematode Radopholus similis

Nematology ◽  
2006 ◽  
Vol 8 (4) ◽  
pp. 627-636 ◽  
Author(s):  
Bjoern Niere ◽  
Shahasi Athman ◽  
Altus Viljoen ◽  
Clifford Gold ◽  
Thomas Dubois ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Mortality Rates ◽  
Antagonistic Activity ◽  
Radopholus Similis ◽  
Biological Control Agents ◽  
Inhibitory Effects ◽  
Culture Filtrates ◽  
Burrowing Nematode ◽  
Nematode Mortality

AbstractRadopholus similis is one of the key pests of banana worldwide. In this study, nine endophytic Fusarium oxysporum isolates were screened for the production of secondary metabolites antagonistic to R. similis in culture. Undiluted and diluted culture filtrates were tested against motile stages and eggs of R. similis. All isolates tested demonstrated in vitro antagonistic activity, causing paralysis of R. similis motile stages. The percentage of paralysed nematodes increased with increase in the length of exposure time to culture filtrates. After 24 h exposure in culture filtrates up to 100% of the treated nematodes were paralysed compared to 26.5% in the control treatments. Nematode mortality rates after 24 h exposure in culture filtrates ranged from 76.4% to 100%. Paralysis was reversible at lower filtrate concentrations. Radopholus similis males were more sensitive to culture filtrates than females. Culture filtrates of all isolates demonstrated inhibitory effects on hatching of R. similis eggs. The results demonstrate the potential for using endophytic F. oxysporum as biological control agents against R. similis and for toxic derivatives from their secondary metabolism to be used as potential nematicides.

scholarly journals MANAGEMENT OF PARTHENIUM WEED USING METABOLITES OF Alternaria japonica

2017 ◽  
Vol 35 (0) ◽  
Author(s):  
A. JAVAID ◽  
T MUBEEN ◽  
U BASHIR ◽  
A SHOAIB
Keyword(s):  
Filter Paper ◽  
Foliar Spray ◽  
Fungal Species ◽  
Herbicidal Activity ◽  
Malt Extract ◽  
Fungal Culture ◽  
Invasive Weed ◽  
Potato Dextrose Broth ◽  
Culture Filtrates ◽  
Parthenium Weed

ABSTRACT Parthenium (Parthenium hysterophorus) is an alien invasive weed infesting many parts of the world including Pakistan. A number of herbicides have been recommended for control of this weed, but these herbicides have issues regarding human health and environmental safety. In the current study, the herbicidal potential of culture filtrates of a fungal species, namely Alternaria japonica, was evaluated against parthenium weed. The fungal species was grown in malt extract broth (MEB) and potato dextrose broth (PDB) for 15 days. Culture filtrates were obtained by passing the materials through muslin cloth followed by filtration through filter paper and then through Millipore filter paper. In laboratory bioassays, the effect of original (X) and diluted (1/2X) filtrates was studied on seed germination, and shoot and root growth of parthenium. In general, fungal culture filtrates prepared in either of the two growth medium significantly reduced germination and seedling growth of parthenium. However, culture filtrates prepared in potato dextrose broth showed greater herbicidal activity than those prepared in malt extract broth. Foliar spray bioassays were carried out by using culture filtrates of A. japonica prepared in potato dextrose broth. In this experiment, 1-, 2- and 3 week old parthenium seedlings were sprayed 4 times with original (X) and concentrated (2X) fungal culture filtrates, with intervals of 4 days. In general, 1 week old parthenium seedlings were highly susceptible to foliar spray of fungal metabolites. The present study concludes that culture filtrates of A. japonica prepared in potato dextrose broth contain potent herbicidal constituents for management of parthenium.

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